Location
Faculty of Science and Biology Centre - Institute of Parasitology,
Branisovska 31, 370 05 Ceske Budejovice, Czech Republic
Branisovska 31, 370 05 Ceske Budejovice, Czech Republic
Date
August 20 - 29th 2008
Organiser
Jana Nebesarova, Biology centre - Institute of Parasitology, Branisovska 31, 370 05 Ceske Budejovice
Phone: +420387775402, fax: +420385300388 (nebe@paru.cas.cz)
Phone: +420387775402, fax: +420385300388 (nebe@paru.cas.cz)
Co-organisers
Gareth Griffiths, EMBL, Postfach 10.2209, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
Ivan Raska, First Faculty of Medicine, Charles University in Prague and Institute of Physiology, Academy of Sciences of the Czech Republic v.v.i., Albertov 4, 12800 Prague 2, Czech Republic
Paul Webster, House Ear Institute, 2100 W. 3rd. St., CA 90057 Los Angeles, US
About the Course
This is a course about the production of thin sections of biological material and their use in studying ultrastructure in the context of molecular cell biology and in obtaining quantitative 3D information about objects from 2D measurements by means of stereology. The course will introduce the techniques of cryosectioning, rapid freezing methods as an alternative to chemical fixation, freeze substitution, resin embedding and stereologic methods for determining volumes of objects, surface areas, a number of particles and mean particle volumes. It will instruct participants in the sectioning of suitably prepared material and will then concentrate on the use of these sections for localizing specific molecules within cells. Ample time will be given to hands on practical work as well as formal and informal discussion of available techniques.
Partnership
