Laboratory of Molecular Biology of Protists

Other

Research projects

• Functional analysis of mitochondrial proteins in Trypanosoma brucei. Grant Agency of the Czech Republic (204/06/1558; P.I.: J. Lukeš; 2006–2008)
• Characterization of a novel protein complex involved in RNA processing and editing in the mitochondrion of Trypanosoma brucei. Grant Agency of the Czech Republic (204/09/1667; P.I.: J. Lukeš; 2009–2012)
• The role of Trypanosoma brucei RNA binding proteins in mitochondrial transcript processing. Grant Agency of the Academy of Sciences of the Czech Republic (IAA500960705; P.I.: J. Lukeš; 2007–2010)
• Mitochondrion of Trypanosoma brucei enters the post-proteomic era. Ministry of Education, Youth and Sports (ME09005; P.I.: J. Lukeš; 2009)
• Construction of the new eukaryotic expression system. Academy of Sciences of the Czech Republic (1QS600220554; P.I.: J. Lukeš; 2005–2008)
• Model organisms as a key to the understanding of functions of disease-related human genes. Ministry of Education, Youth and Sports (2B06129; P.I.: J. Lukeš; 2006–2011)
• Genomic, transcriptomic and proteomic view of a photosynthetic algae Chromera velia, a evolutionary ‘missing link’ to the human malaria parasites. Global collaborative grant, King Abdullah University of Science and Technology, Saudi Arabia (P.I.: A. Pain and J. Lukeš; 2010-2012)

Current Events

• RNA Club 2011

 

List of publications (by date - from the newest)

2012

• Kořený L., Sobotka R., Kovářová J., Gnipová A., Flegontov P., Horváth A., Oborník M., Ayala F.J. & Lukeš J. (2012) Aerobic kinetoplastid flagellate Phytomonas does not require heme for viability. PNAS 109: 3808–3813
• Oborník M., Modrý D., Lukeš M., Černotíková-Stříbrná E., Cihlář J., Tesařová M., Kotabová E., Vancová M., Prášil O., Lukeš J. (2012) Morphology, Ultrastructure and Life Cycle of Vitrella brassicaformis n. sp., n. gen., a Novel Chromerid from the Great Barrier Reef. Protist 163: 306-323.
• Docampo R, Lukeš J. (2012) Trypanosomes and the solution to a 50-year mitochondrial calcium mystery. Trends in Parasitology 28: 31-37.
• Jirků Milan, Pomajbíková K., Petrželková K.J., Hůzová Z., Modrý D., Lukeš J. (2012) Detection of Plasmodium spp. in human feces. Emerging Infectious Diseases 18: 634–636.

2011

• Flegontov P., Gray M.W., Burger G. & Lukeš J. (2011) Gene fragmentation: a key to mitochondrial genome evolution in Euglenozoa?. Curr Genet 57: 225–232
• Long S., Changmai P., Tsaousis A.D., Skalický T., Verner Z., Wen Y.-Z., Roger A.J. & Lukeš J. (2011) Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues. Mol. Microbiol. 81: 1403–1418
• Szabová J., Růžička P., Verner Z., Hampl V. & Lukeš J. (2011) Experimental Examination of EFL and MATX Eukaryotic Horizontal Gene Transfers: Coexistence of Mutually Exclusive Transcripts Predates Functional Rescue. Mol. Biol. Evol. 28: 2371–2378
• Alfonzo J.D. & Lukeš J. (2011) Assembling Fe/S-clusters and modifying tRNAs: ancient co-factors meet ancient adaptors. Trends Parasitol. 27: 234–237
• Tanifuji G, Kim E., Onodera N.T., Gibeault R., Dlutek M., Cawthorn R.J., Fiala I., Lukeš J., Greenwood S.J. & Archibald J.M. (2011) Genomic Characterization of Neoparamoeba pemaquidensis (Amoebozoa) and Its Kinetoplastid Endosymbiont. Eukaryot. Cell 10: 1143–1146
• Lukeš J., Archibald J.M., Keeling P.J., Doolittle W.F. & Gray M.W. (2011) How a Neutral Evolutionary Ratchet Can Build Cellular Complexity. IUBMB. 63(7), 528–537
• Doolittle W.F., Lukeš J., Archibald J.M., Keeling P.J. & Gray M.W. (2011) Does constructive neutral evolution play an important role in the origin of cellular complexity? *BioEssays* 33, 427-429
• Michelle L. Ammerman, Hassan Hashimi, Lucie Novotná, Zdeňka Čičová, Sarah M. Mcevoy, Julius Lukeš & Laurie K. Read (2011) MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process. RNA. 17, 865-877
• Paris Z., Hashimi H., Lun S., Alfonzo J.D. & Lukeš J. (2011) Futile import of tRNAs and proteins into the mitochondrion of Trypanosoma brucei evansi. Mol. Biochem. Parasitol. 176, 116-120
• Verner Z., Čermáková P., Škodová I., Kriegová E., Horváth A. & Lukeš J. (2011) Complex I (NADH:ubiquinone oxidoreductase) is active in but non-essential for procyclic Trypanosoma brucei. Mol. Biochem. Parasitol. 175, 196-200
• Richterová L., Vávrová Z. & Lukeš J. (2011) DEAD-box RNA helicase is dispensable for mitochondrial translation in Trypanosoma brucei. Experimental Parasitol. 127, 300-303.
• Vlček Č., Marande W., Teijeiro S., Lukeš J. & Burger G. (2011) Systematically fragmented genes in a multipartite mitochondrial genome. Nucl. Acids Res. 39, 979-988
• Oborník M., Vancová M., Lai D.-H., Janouškovec J., Keeling P.J. & Lukeš J. (2011) Morphology and ultrastructure of multiple life cycle stages of the photosynthetic relative of Apicomplexa, Chromera velia. Protist 162, 115-130.

2010

• Lun Z.-R., Lai D.-H., Li F.-J., Lukeš J. & Ayala F.J. (2010) Trypanosoma brucei: two steps to spread out from Africa. Trends Parasitol. 26, 434-437.
• Lukeš J., Hashimi H., Verner Z. & Čičová Z. (2010) The remarkable mitochondrion of trypanosomes and related flagellates. In: Structures and organelles in pathogenic protists (ed. De Souza W.). Springer-Verlag Berlin, Heidelberg; pp. 228-252
• Janouškovec J., Horák A., Oborník M., Lukeš J. & Keeling P.J. (2010) A common red algal origin of the apicomplexan, dinoflagellate and heterokont plastids. Proc. Natl. Acad. Sci. USA 107, 10949-10954
• Gray M.W., Lukeš J., Archibald J.M., Keeling P.J. & Doolittle W.F. (2010) Irremediable complexity? Science 330, 920-921.
• Lukeš J. & Raper J. (2010) Prophylactic antiparasitic transgenosis for human parasitic disease? Mol. Therapy 18, 1745-1747
• Gazdag M., Cirstea I., Breitling R., Lukeš J., Blankenfeldt W. & Alexandrov K. (2010) Purification and crystallization of human Cu/Zn superoxide dismutase recombinantly produced in the protozoan Leishmania tarentolae. Acta Crystalograph. F66, 871-877
• Paris Z., Changmai P., Rubio M.A.T., Zíková A., Stuart K.D., Alfonzo J.D. & Lukeš J. (2010) The Fe/S cluster assembly protein Isd11 is essential for tRNA thiolation in Trypanosoma brucei. J. Biol. Chem. 285, 22394-22402
• Týč J., Long S., Jirků M. & Lukeš J. (2010) YCF45 protein, usually associated with plastids, is targeted into the mitochondrion of Trypanosoma brucei. Mol. Biochem. Parasitol. 173, 43-47
• Kořený L., Lukeš J. & Oborník M. (2010) Evolution of the heme synthetic pathway in kinetoplastid flagellates: An essential pathway that is not essential after all? Int. J. Parasitol. 40, 149-156
• Keeling P.J., Leander B.S. & Lukeš J. (2010) Reply to Speijer et al.: Does complexity necessarily arise from selective advantage? *Proc. Natl. Acad. Sci. USA* 107, E26
• Maslov D.A., Yurchenko V.Y., Jirků M. & Lukeš J. (2010) Two new species of trypanosomatid parasites isolated from Heteroptera in Costa Rica. J. Euk. Microbiol. 57, 177-188.
• Verner Z., Paris Z. & Lukeš J.(2010) Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei. Parasitol. Res. 106, 1241-1244.
• Simpson L., Aphasizhev R., Lukeš J. & Cruz-Reyes J. (2010) Guide to the nomenclature of kinetoplastid editing: A proposal. Protist 161, 2-6.
• Poliak P., Van Hoewyk D., Oborník M., Zíková A., Stuart K.D., Tachezy J., Pilon M. & Lukeš J. (2010) Functions and cellular localization of cysteine desulfurase and selenocysteine lyase in Trypanosoma brucei. FEBS J. 277, 383-393.
• Votýpka J., Maslov D.A., Yurchenko V., Jirků M., Kment P., Lun Z.-R. & Lukeš J. (2010) Probing into the diversity of trypanosomatid flagellates parasitizing insect hosts in South-West China reveals both endemism and global distribution. Mol. Phyl. Evol. 54, 243-253
• Týč J., Faktorová D., Kriegová E., Jirků M., Vávrová Z., Maslov D.A. & Lukeš J. (2010) Probing for primary functions of prohibitin in Trypanosoma brucei. Int. J. Parasitol. 40, 73-83
• Hashimi H., Benkovičová V., Čermáková P., Lai D.-H., Horváth A. & Lukeš J. (2010) The assembly of F1FO-ATP synthase is disrupted upon interference of RNA editing in Trypanosoma brucei. Int. J. Parasitol. 40, 45-54

2009

• Yurchenko V.Y., Lukeš J., Jirků M. & Maslov D.A. (2009) Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera. Int. J. Syst. Evol. Microbiol. 59, 893–909
• Wohlgamuth-Benedum J.M., Rubio M.A.T., Paris Z., Long S., Poliak P., Lukeš J. & Alfonzo J.D. (2009) Thiolation controls cytoplasmic tRNA stability and acts as a negative determinant for tRNA editing in mitochondria. J. Biol. Chem. 284, 23947-23953
• Paris Z., Rubio, M.A.T., Lukeš J. & Alfonzo J.D. (2009) Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein. RNA 15, 1398-1406
• Lukeš J., Leander B.S. & Keeling P.J. (2009) Cascades of convergent evolution: The corresponding evolutionary histories of euglenozoans and dinoflagellates. Proc. Natl. Acad. Sci. USA 106, 9963-9970
• Gile G.H., Faktorová D., Castlejohn C.A., Burger G., Lang B.F., Farmer M.A., Lukeš J. & Keeling P.J. (2009) Distribution and Phylogeny of EFL and EF-1α in Euglenozoa Suggest Ancestral Co-Occurrence Followed by Differential Loss. PLoS ONE. 4, e5162
• Hashimi H., Čičová Z., Novotná L., Wen Y.-Z. & Lukeš J. (2009) Kinetoplastid guide RNA biogenesis is dependent on subunits of the mitochondrial RNA binding complex 1 and mitochondrial RNA polymerase. RNA. 15, 588-599
• Lai D.-H., Wang Q.-P., Li Z., Lukeš J. & Lun Z.-R. (2009) Evolution of the serum resistance-associated SRA gene in African trypanosomes. Chinese Science Bulletin. 54, 1275-1278
• Foldynová-Trantírková S., Matulová J., Dötsch V., Löhr F., Cirstea I., Alexandov K., Breitling R., Lukeš J. & Trantírek L. (2009) A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae. Journal of Biomolecular Structure & Dynamics. 26, 755-761
• Jirků M., Jirků M., Oborník M., Lukeš J. & Modrý D. (2009) A Model for Taxonomic Work on Homoxenous Coccidia: Redescription, Host Specificity, and Molecular Phylogeny of Eimeria ranae Dobell, 1909, with a Review of Anuran-Host Eimeria (Apicomplexa: Eimeriorina). J. Euk. Microbiol. 56, 39-51
• Jirků M., Jirků M., Oborník M., Lukeš J. & Modrý D. (2009) Goussia Labbé, 1896 (Apicomplexa, Eimeriorina) in Amphibia: Diversity, Biology, Molecular Phylogeny and Comments on the Status of the Genus. Protist 160, 123-136
• Oborník M., Janouškovec J., Chrudimský T. & Lukeš J. (2009) Evolution of the apicoplast and its hosts: From heterotrophy to autotrophy and back again. Int. J. Parasitol. 39, 1-12.

2008

• Long S., Vávrová Z. & Lukeš J. (2008) The import and function of diatom and plant frataxins in the mitochondrion of Trypanosoma brucei. Mol. Biochem. Parasitol. 162, 100-104
• Long S., Jirků M., Ayala F.J. & Lukeš J. (2008) Mitochondrial localization but not processing of human frataxin is essential for a rescue of frataxin deficiency in the excavate flagellate Trypanosoma brucei. Proc. Natl. Acad. Sci. USA 105, 13468-13473
• Long S., Jirků M., Mach J., Ginger M.L., Sutak R., Richardson D., Tachezy J. & Lukeš J. (2008) Ancestral roles of eukaryotic frataxin: mitochondrial frataxin function and heterologous expression of hydrogenosomal /Trichomonas/ homologs in trypanosomes. Mol. Microbiol. 69, 94-109
• Hashimi H., Zíková A., Panigrahi A.K., Stuart K.D. & Lukeš J. (2008) TbRGG1, a component of a novel multi-protein komplex involved in kinetoplastid RNA editing. RNA 14, 970-980
• Lai D.-H., Hashimi H., Lun Z.-R., Ayala F.J. & Lukeš J. (2008) Adaptation of Trypanosoma brucei to gradual loss of kinetoplast DNA: T. equiperdum and T. evansi are petite mutants of T. brucei. Proc. Natl. Acad. Sci. USA 105, 1999-2004
• Zíková A., Kopečná J., Schumacher M.A., Stuart K.D., Trantírek L. & Lukeš J. (2008) structure and function of the native and recombinant mitochondrial MRP1/MRP2 complex from Trypanosoma brucei. Int. J. Parasitol. 38, 901-912
• Guler J., Kriegová E., Smith T.K., Lukeš J. & Englund P.T. (2008) Mitochondrial fatty acid synthesis is required for normal mitochondrial morphology and function in Trypanosoma brucei. Mol. Microbiol. 67, 1125-1142
• Chocholová E, Jirků M. & Lukeš J. (2008) A diagnostic assay based on variable intergenic region distinguishes between Leishmania donovani and Leishmania infantum. Folia Parasitol. 55, 75-78

2007

• Yurchenko V.A., Lukeš J., Tesařová M., Jirků M. & Maslov D.A. (2007) Morphological discordance of the new trypanosomatid species phylogenetically associated with the genus Crithidia. Protist 159, 99-114
• Zamudio J.R., Mittra B., Foldynová-Trantírková S., Zeiner G.M., Lukeš J., Bujnicky J.M., Sturm N.R. & Campbell D.A. (2007) The 2’-O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma brucei. Mol. Cell. Biol. 27, 6084-6092
• Herder S., Votýpka J., Jirků M., Rádrová J., Janzen C.J.& Lukeš J. (2007) Trypanosoma brucei 29-13 strain is inducible in but not permissive for the tsetse fly vector. Exp. Parasitol.
• Lukeš J., Mauricio I.L., Schönian G., Dujardin J.-C., Soteriadou K., Dedet J.-P., Kuhls K., Quispe Tintaya W., Jirků M., Chocholová E., Haralambous C., Pratlong F., Oborník M., Horák A., Ayala F.J. & Miles M.A. (2007) Evolutionary and geographical history of the Leishmania donovani complex with a revision of current taxonomy. Proc. Natl. Acad. Sci. USA 104, 9375-9380
• Čermáková P., Verner Z., Man P., Lukeš J. & Horváth A. (2007) Characterization of the NADH: ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida). FEBS J. 274, 3150-3158
• Roy J., Faktorová D., Lukeš J. & Burger G. (2007) Unusual mitochondrial genome structure throughout Euglenozoa. Protist 158, 385-396
• Sturm N.R., Dollet M., Lukeš J. & Campbell D.A. (2007) Meaningful sub-division of plant trypanosomes (Phytomonas spp.) based on minicircle conserved region analysis. Infect., Genet. & Evol. 7, 570-576
• Svobodová M., Zídková L., Čepička I., Oborník M., Lukeš J. & Votýpka J. (2007) Sergeia podlipaevi gen. nov., sp. nov. (Trypanosomatidae, Kinetoplastida): a new parasite of the biting midges (Ceratopogonidae, Diptera). Int. J. Syst. Evol. Microbiol. 57, 423-432
• Roy J., Faktorová D., Benada O., Lukeš J. & Burger G. (2007) Description of Rhynchopus euleeides n. sp. (Diplonemea), a free-living, marine euglenozoan. J. Euk. Microbiol. 54, 137-145
• Zemanová E., Jirků M., Mauricio I.L., Horák A., Miles M.A. & Lukeš J. (2007) The Leishmania donovani complex: Genotypes of five metabolic enzymes (ICD, ME, MPI, G6PDH, FH), new targets for multilocus sequence typing. Int. J. Parasitol. 37, 149-160

2006

• Vávra J., Horák A., Modrý D., Lukeš J. & Koudela B. (2006) Trachipleistophora extenrec, n. sp., a new microsporidian (Fungi: Microsporidia) infecting mammals. J. Euk. Microbiol. 3, 464-476
• Yurchenko V.A., Lukeš J., Jirků M., Zeledón R. & Maslov D.A. (2006) Leptomonas costaricensis sp.n. (Kinetoplastea: Trypanosomatidae), a member of the novel phylogenetic group of insect trypanosomatids closely related to the genus Leishmania. Parasitology 133, 537-546
• Šmíd O., Horáková E., Vilímová V., Hrdý I., Cammack R., Horváth A., Lukeš J. & Tachezy J. (2006) Knock-downs of mitochondrial iron-sulfur cluster assembly proteins IscS and IscU
• Schumacher M.A., Karamooz E., Zíková A., Trantírek L. & Lukeš J. (2006) Crystal structures of Trypanosoma brucei MRP1/MRP2 guide-RNA-binding complex reveals RNA matchmaking mechanism. Cell 126, 701-711
• Zíková A., Horáková E., Jirků M., Dunajčíková P. & Lukeš J. (2006) The effect of down- regulation of mitochondrial RNA-binding proteins MRP1 and MRP2 on respiratory complexes in procyclic Trypanosoma brucei. Mol. Biochem. Parasitol. 149, 65-73
• Wijová M., Moravec F., Horák A. & Lukeš J. (2006) Evolutionary relationships of Spirurina (Nematoda: Chromadorea: Rhabditida) with special emphasis on dracunculoid nematodes inferred from SSU rRNA gene sequences. Int. J. Parasitol. 36, 1067-1075
• Lukeš J., Paris Z., Regmi S., Breitling R., Mureev S., Kushnir S., Pyatkov K., Jirků M. & Alexandrov K.A. (2006) Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5‘ sequence context. Mol. Biochem. Parasitol. 148, 125-132
• Mauricio I.L., Yeo M., Baghaei M., Doto D., Silk R., Pratlong F., Zemanová E., Dedet J.-P., Lukeš J. & Miles M.A. (2006) Towards multi locus sequence typing in the Leishmania donovani complex: resolving genotypes and haplotypes for five polymorphic metabolic enzymes (ASAT, GP1, NH1, NH2, PGD). Int. J. Parasitol. 36, 757-769
• Simpson A.G.B., Stevens J.R. & Lukeš J. (2006) The evolution and diversity of kinetoplastid flagellates. Trends Parasitol. 22, 168-174
• Jirků M., Zemanová E., Al-Jawabreh A., Schönian G. & Lukeš J. (2006) Development of a direct species-specific PCR assay for differential diagnosis of Leishmania tropica.
• Kopečná J., Jirků M., Oborník M., Tokarev Z.S., Lukeš J. & Modrý D. (2006) Phylogenetic analysis of coccidian parasites from invertebrates: Search for missing links. Protist 157, 173-183
• Li F.-J., Lai D.-H., Lukeš J., Chen X.-G. & Lun Z.-R. (2006) Doubts about Trypanosoma equiperdum strains classed as Trypanosoma brucei or Trypanosoma evansi.
• Yurchenko V., Lukeš J., Xu X. & Maslov D.A. (2006) An integrated morphological and molecular approach to a new species description in the Trypanosomatidae: the case of Leptomonas podlipaevi n.sp., a parasite of Boisea rubrolineata (Hemiptera: Rhopalidae). J. Euk. Microbiol. 53, 1-9

2005

• Lukeš J., Hashimi H. & Zíková A. (2005) Unexplained complexity of the mitochondrial genome and transcriptome in kinetoplastid flagellates. Curr. Genet. 48, 277-299
• Horváth A., Horáková E., Dunajčíková P., Verner Z., Pravdová E., Šlapetová I., Cuninková L. & Lukeš J. (2005) Down-regulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei. Mol. Microbiol. 58, 116-130
• Marande W., Lukeš J. & Burger G. (2005) Unique mitochondrial genome structure in diplonemids, the sister group of kinetoplastids. Eukaryot. Cell 4, 1137-1146
• Lukeš J., Horák A. & Scholz T. (2005) Helminth genome projects: all or nothing.
• Foldynová-Trantírková S., Paris Z., Sturm N.R., Campbell D.A. & Lukeš J. (2005) The Trypanosoma brucei La protein is a candidate poly(U) shield protein that impacts spliced leader RNA maturation and tRNA intron removal. Int. J. Parasitol. 35, 359-366
• Wijová M., Moravec F., Horák A., Modrý D. & Lukeš J. (2005) Phylogenetic position of Dracunculus medinensis and some related nematodes inferred from 18S rRNA. Parasitol. Res. 96, 133-135
• Vondrušková E., van den Burg J., Zíková A., Ernst N.L., Stuart K., Benne R. & Lukeš J. (2005) RNA interference analyses suggest a transcript-specific regulatory role for MRP1 and MRP2 in RNA editing and other RNA processing in Trypanosoma brucei. J. Biol. Chem. 280, 2429-2438

2004

• Votýpka J., Lukeš J. & Oborník M. (2004) Phylogenetic relationship of Trypanosoma corvi and other Old World avian trypanosomes. Acta Protozool. 43, 225-231
• Zemanová E., Jirků M., Mauricio I.L., Miles M.A. & Lukeš J. (2004) Genetic polymorphism within the Leishmania donovani complex: correlation with geographical origin.
• Podlipaev S.A., Sturm N.R., Fiala I., Fernandes O., Westenberger S.J., Dollet M., Campbell D.A. & Lukeš J. (2004) Diversity of insect trypanosomatids assessed from the spliced leader RNA and 5S rRNA genes and intergenic regions. J. Euk. Microbiol. 51, 283-290
• Avliyakulov N.K., Lukeš J. & Ray D.S. (2004). Mitochondrial histone-like DNA-binding proteins are essential for normal cell growth and mitochondrial function in Crithidia fasciculata. Eukaryot. Cell 3, 518-526
• Podlipaev S.A., Votýpka J., Jirků M., Svobodová M. & Lukeš J. (2004). Herpetomonas ztiplika n.sp. (Kinetoplastida: Trypanosomatidae) – parasite of the blood-sucking biting midge Culicoides kibunensis Tokunaga, 1937 (Diptera: Ceratopogonidae). J. Parasitol. 90, 342-347
• Zeiner G., Foldynová S., Sturm N.R., Lukeš J. & Campbell D.A. (2004). SmD1 is required for spliced leader RNA biogenesis. Eukaryot. Cell 3, 241-244

2003

• Dávila A.M.R. & Lukeš J. (2003) Towards a framework for the evolutionary genomics of Kinetoplastids: what kind of data and how much? Kinet. Biol. & Dis. 2, 16-19
• Zíková A., Vancová M., Jirků M. & Lukeš J. (2003). Cruzella marina (Bodonina, Kinetoplastida): non-catenated structure of poly-kinetoplast DNA. Exp. Parasitol. 104: 159-161
• Gažiová I. & Lukeš J. (2003). Mitochondrial and nuclear localization of topoisomerase II in the flagellate Bodo saltans (Kinetoplastida), a species with non-catenated kinetoplast DNA. J. Biol. Chem. 278: 10900-10907
• Šlapeta J.R., Modrý D., Votýpka J., Jirků M., Lukeš J. & Koudela B. (2003). Evolutionary relationships among cyst-forming coccidia Sarcocystis spp. (Alveolata: Apicomplexa: Coccidea) in endemic African tree vipers and perspective for evolution of heteroxenous life cycle. Mol. Phyl. Evol. 27: 464-475
• Dyková I., Fiala I., Lom J. & Lukeš J. (2003). Perkinsiella amoebae-like endosymbionts of Neoparamoeba spp., relatives of the kinetoplastid Ichthyobodo. Europ. J. Protistol. 39: 37-52

2002

• Maslov D.A., Zíková A., Kyselová I. & Lukeš J. (2002). A novel nuclear-encoded putative subunit of the cytochrome c oxidase complex in trypanosomatids. Mol. Biochem. Parasitol. 125: 113-125
• Lukeš J., Guilbride D.L., Votýpka J., Zíková A., Benne R. & Englund P.T. (2002). The kinetoplast DNA network: Evolution of an improbable structure. Eukaryot. Cell 1: 495-502
• Modrý D., Šlapeta J.R., Kyselová I., Hořejš R., Lukeš J., Koudela B. (2002) Dog shedding oocysts of Neospora caninum: diagnostic and phylogenetic approach to the taxonomy of related organisms. Vet. Parasitol. 109: 157-167
• Simpson A.G.B., Lukeš J., Roger A.J. (2002). The evolutionary history of kinetoplastids and their kinetoplast. Mol. Biol. Evol. 19: 2071-2083
• Votýpka J., Oborník M., Volf P., Svobodová M. & Lukeš J. (2002) Trypanosoma avium of raptors (Falconiformes): Phylogeny and identification of vectors. Parasitology 125: 253-263
• Šlapeta J.R., Kyselová I., Richardson A.O., Modrý D., Lukeš J. (2002). Phylogeny and sequence variability of the Sarcocystis singaporensis Zaman and Colley (1975) 1976 ssrDNA. Parasitol. Res. 88: 810-815
• Šlapeta J. R., Koudela B., Votýpka J., Modrý D., Hořejš R. & Lukeš J. (2002). Coprodiagnosis of Hammondia heydorni in dogs by PCR based on ITS 1 rRNA: Differentiation from morphologically indistinguishable oocysts of Neospora caninum. Vet. J. 163: 147-154
• Horváth A., Neboháčová M., Lukeš J. & Maslov D.A. (2002). Unusual polypeptide synthesis in the kinetoplast-mitochondria from Leishmania tarentolae. Identification of individual de novo translation products. J. Biol. Chem. 277: 7222-7230
• Oborník M., Van de Peer Y., Hypša V., Frickey T., Šlapeta J.R., Meyer A. & Lukeš J. (2002) Phylogenetic analyses suggest a possible lateral gene transfer from the mitochondrion to the apicoplast. Gene 285: 109-118
• Oborník M., Jirků M., Šlapeta J.R., Modrý D., Koudela B. & Lukeš J. (2002). Notes on coccidian phylogeny based on the apicoplast small subunit ribosomal RNA. Parasitol. Res. 88: 360-363
• Jirků M., Modrý D., Šlapeta J.R., Koudela B. & Lukeš J. (2002). The phylogeny of Goussia and Choleoeimeria (Apicomplexa; Eimeriorina) and the evolution of excystation structures in coccidia. Protist 153: 380-389

2001

• Santana D.M., Lukeš, J., Sturm N.R. & Campbell D.A. (2001). Two sequence classes of kinetoplastid 5S ribosomal RNA gene revealed among bodonid spliced leader RNA gene arrays. FEMS Lett. 204: 33-237
• Votýpka J., Ray D.S. & Lukeš J. (2001). Crithidia fasciculata: a test for genetic exchange. Exp. Parasitol. 99: 104-107
• Lukeš J., Hines J.C., Evans C.J., Avliyakulov N.K., Prabhu V.P., Chen J. & Ray D.S. (2001). Disruption of the Crithidia fasciculata KAP1 gene results in extensive structural rearrangement of the kinetoplast disc. Mol. Biochem. Parasitol. 117:179-186
• Dyková I., Kyselová I., Pecková H., Oborník M. & Lukeš J. (2001). Identity of Naegleria strains isolated from organs of freshwater fishes. Dis. aquat. Org. 46:115-121
• Modrý D., Šlapeta J. R., Jirků M., Oborník M., Lukeš J. & Koudela B. (2001) Phylogentic position of a renal coccidium of the European green frog, Isospora lieberkuehni Labbe, 1894 (Apicomplexa: Sarcocystidae: Toxoplasmatinae) and the taxonomic implications. Int. J. Syst. Evol. Microbiol. 51:767-772
• Šlapeta J. R., Modrý D., Votýpka J., Jirků M., Oborník M., Lukeš J. & Koudela B. (2001) Eimeria telekii n. sp. (Apicomplexa: Coccidia) from Lemniscomys striatus (Rodentia: Muridae): morphology, pathology and phylogeny. Parasitology 122: 133-145
• Maslov D.A., Podlipaev S.A. & Lukeš J. (2001). Phylogeny of the Kinetoplastida: Taxonomic problems and insights into the evolution of parasitism. Mem. Inst. Osw. Cruz 96: 397-402
• Štolba P., Jirků M. & Lukeš J. (2001) Polykinetoplast DNA structure in Dimastigella trypaniformis and Dimastigella mimosa (Kinetoplastida). Mol. Biochem. Parasitol. 13: 323-326
• Šlapeta J.R., Modrý D., Votýpka J., Jirků M., Koudela B. & Lukeš J. (2001). Multiple origin of the dihomoxenous life cycle in sarcosporidia. Int. J. Parasitol. 31: 413-417

2000

• Lukeš J. & Votýpka J. (2000). Trypanosoma avium: Novel features of the kinetoplast structure. Exp. Parasitol. 96: 178-181
• Kodedová I., Doležel D., Broučková M., Jirků M., Hypša V., Lukeš J. & Scholz T. (2000). On the phylogenetic position of the Caryophyllidea, Pseudophyllidea and Proteocephalidea (Eucestoda) inferred from 18S rRNA. Int. J. Parasitol. 30: 1109-1113
• Doležel D., Jirků M., Maslov D.A. & Lukeš J. (2000) Phylogeny of the bodonid flagellates (Kinetoplastida) based on small subunit rRNA gene sequences. Int. J. Syst. Evol. Microbiol. 50: 1943-1951
• Yurchenko V., Kolesnikov A.A. & Lukeš J. (2000) Phylogenetic analysis of Trypanosomatina (Protozoa: Kinetoplastida) based on minicircle conserved regions. Folia Parasitol. 47:1-5
• Avliyakulov N., Lukeš J., Kajava A.V., Liedberg B., Lundstrom I. & Svensson S.P.S. (2000) Suramin blocks nucleotide triphosphate binding to ribosomal protein L3 from Trypanoplasma borreli. Europ. J. Biochem. 267: 1723-1731
• Lukeš J. & Maslov D.A. (2000) Unexpectedly high variability of histone H4 gene in Leishmania. Parasitol. Res. 86: 259-261
• Blom D., de Haan A., van den Burg J., van den Berg M., Sloof P., Jirků M., Lukeš J. & Benne R. (2000) Mitochondrial minicircles in the free-living bodonid Bodo saltans contain two gRNA gene cassettes and are not found in large networks. RNA 6: 1-15

1999

• Doležel D., Koudela B., Jirků M., Hypša V., Oborník M., Votýpka J.,Modrý D., Šlapeta J.R. & Lukeš J. (1999) Phylogenetic analysis of Sarcocystis spp. of mammals and reptiles support the coevolution of Sarcocystis spp. with their final hosts. Int. J. Parasitol.. 29: 795-798
• Yurchenko V., Hobza R., Benada O. & Lukeš J. (1999) Trypanosoma avium: Large minicircles in the kinetoplast DNA. Exp. Parasitol. 92: 215-218

1998

• Maslov D.A. & Lukeš J. (1998) Searching for a tree which can be trusted. Parasitol. Today 14: 334
• Hollar L., Lukeš J. & Maslov D.A. (1998) Monophyly of endosymbiont-containing trypanosomatids: Phylogeny versus Taxonomy. J. Euk. Microbiol. 45: 293-297
• Votýpka J., Hypša V., Jirků M., Flegr J., Vávra J. & Lukeš J. (1998) Molecular phylogenetic relatedness of Frenkelia spp. (Protozoa, Apicomplexa) to Sarcocystis falcatula Stiles 1893: Is the genus Sarcocystis paraphyletic? J. Euk. Microbiol. 45: 137-141
• Lukeš J., Jirků M., Avliyakulov N. & Benada O. (1998) Pankinetoplast DNA structure in a primitive bodonid flagellate, Cryptobia helicis. EMBO J. 17: 838-846
• Blom D., de Haan A., van den Berg M., Sloof P., Jirků M., Lukeš J. & Benne R. (1998) RNA editing in the free-living bodonid Bodo saltans. Nucleic Acids Res. 26: 1205-1213

1997

• Král’ová I., van de Peer Y., Jirků M., van Ranst M., Scholz T. & Lukeš J. (1997) Phylogenetic analysis of a fish tapeworm, Proteocephalus exiguus, based on the small subunit rRNA gene. Mol. Biochem. Parasitol. 84: 263-266
• Lukeš J., Jirků M., Doležel D., Král’ová I., Hollar L. & Maslov D.A. (1997) Analysis of ribosomal RNA genes suggests that trypanosomes are monophyletic. J. Mol. Evol. 44: 521-527

1996

• Maslov D.A., Lukeš J., Jirků M. & Simpson L. (1996) Phylogeny of trypanosomes as inferred from the small and large subunit rRNAs: Implications for the evolution of parasitism in the trypanosomatid protozoa. Mol. Biochem. Parasitol. 75:197-205

1995

• Jirků M., Kolesnikov A.A., Benada O. & Lukeš J. (1995) Marine fish and ray trypanosomes have large kinetoplast minicircle DNA. Mol. Biochem. Parasitol. 73: 279-283
• Lukeš J. (1995) Goussia chalupskyi n.sp. (Apicomplexa: Eimeriidae) from the chub Leuciscus cephalus (Cyprinidae). Syst. Parasitol. 30:27-30
• Kolesnikov A.A., Jirků M., Pecková H., Polák A., Maslov D.A., Lukeš J. (1995) Analysis of kinetoplast DNA of freshwater fish trypanosomes. Folia Parasitol. 40: 424-428

1994

• Lukeš J., Arts G-J., van den Burg J., de Haan A., Opperdoes F., Sloof P. & Benne R. (1994) Novel pattern of editing regions in mitochondrial transcripts of the cryptobiid Trypanoplasma borreli. EMBO J. 13: 5086-5098
• Lukeš J. (1994) A giant merogonial stage (Apicomplexa, Coccidia) in the intestine of Blicca bjoerkna (L.)(Cyprinidae). Parasite 1: 393-395
• Lukeš J. (1994) Morphology of life cycle of Goussia bohemica n.sp. (Apicomplexa, Eimeriidae), a parasite of the goblet and epithelial cells of the gudgeon (Gobio gobio L.). Europ. J. Protistol. 30: 68-74

1993

• Lukeš J., Volf P. & Lom J. (1993) Detection of chitin in spores of Myxobolus muelleri and M. subepithelialis (Myxosporea, Myxozoa). Parasitol. Res. 79: 439-440
• Lukeš J. (1993) A coccidian (Apicomplexa, Eimeriidae) with extracytoplasmally located stages in kidney tubules of golden carp (Carassius auratus gibelio L.)(Cyprinidae). Folia Parasitol. 40: 1-7

1992

• Lukeš J. & Starý V. (1992) Ultrastructure of life cycle stages of Goussia janae (Apicomplexa, Eimeriidae) and X-ray microanalysis of accompanying precipitates. Can. J. Zool. 70: 2382-2397
• Zajíček P. & Lukeš J. (1992) Differentiation of freshwater and marine fish trypanosomes by lectin agglutinability. Folia Parasitol. 39: 195-200
• Lukeš J. (1992) Life cycle of Goussia pannonica (Molnár, 1989) (Apicomplexa, Eimeriorina), an extracytoplasmic coccidium from the white bream, Blicca bjoerkna. J. Protozool. 39: 484-494
• Lukeš J. & Kepr T. (1992) Eimeria lydiae n.sp.(Apicomplexa, Eimeriidae), a parasite of the brown trout, Salmo trutta m. fario (Salmonidae). Arch. Protistenkd. 141: 219-222

1991

• Lukeš J. & Vávra J. (1991) Microsporidium aedium n.sp., from the mosquito Aedes cantans. J. Invertebr. Pathol. 58: 274-276
• Lukeš J., Steinhagen D. & Körting W. (1991) Goussia carpelli (Apicomplexa, Eimeriorina) from cyprinid fish: Field observations and infection experiments. Angew. Parasitol. 32:149-153

1990

• Steinhagen D., Lukeš J. & Körting W. (1990) Ultrastructural observations on gamogonic stages of Goussia subepithelialis (Apicomplexa, Coccidia) from common carp Cyprinus carpio. Dis. aquat. Org. 9: 31-36
• Lukeš J. & Dyková I. (1990) Goussia janae n.sp. (Apicomplexa,Eimeriorina) in dace Leuciscus leuciscus and chub L. cephalus. Dis. aquat. Org. 8: 85-90
• Lukeš J. & Vávra J. (1990) Life cycle of Amblyospora weiseri n.sp. (Microsporidia) in Aedes cantans (Diptera, Culicidae). Europ. J. Protistol. 25: 200-208
• Volf P., Lukeš J. & Srp V. (1990) Study on the population of the warble fly, Oestromyia leporina (Pallas 1778)(Diptera, Hypodermatidae) in Bohemia. Folia Parasitol. 37: 187-190
• Lukeš J. & Vávra J. (1990) Life cycle of Amblyospora weiseri n.sp. (Microsporidia) in Aedes cantans (Diptera, Culicidae). Europ. J. Protistol. 25: 200-208

Sections > Molecular Parasitology > Laboratory of Molecular Biology of Protists > Other