prof. RNDr. Libor Grubhoffer, CSc., Hon. D.Sc.

Job Position: Head of Laboratory - Laboratory of Molecular Ecology of Vectors and Pathogens

Contact details

Phone: +420 387775050, +420 387775456
E-mail: libor.grubhoffer@bc.cas.cz
Web: liborex@paru.cas.cz
Room: 212, 312

Description

Research interests

  • molecular and cellular factors involved in the mechanism of pathogen transmission by ticks and of the tick immunity
  • Lyme borreliosis spirochaetes and tick-borne encephalitis virus with respect to protein-carbohydrate interactions, and their interaction with the inner environment of the tick Ixodes ricinus
Curriculum vitae

University Education

  • 1976-1981: Department of Biochemistry, Faculty of Science, Charles University, Prague, the Czech Republic. Area of study: biochemistry of lectins, and their biological significance at the cellular level (supervisor Professor Jan Kocourek). Isolation and characterization of the lectins from the seeds of Vicia villosa.
  • 1982: RNDr. (equivalent to M.Sc.) biochemistry, Faculty of Science, Charles University, Prague, the Czech Republic.
  • 1982-1983: Research Assistant in the Laboratory of Chemical Mutagenesis at the Research Institute of Organic Syntheses, Pardubice, Czech Republic.

Postgraduate Study

  • 1983-1986: Ph.D. Research on the antigenic structures of mumps virus (Paramyxoviridae) with the respect to their physico-chemical, immunochemical, and biological properties thesis under the guidence of Professor Dimitrij Slonim in the Department of Preparative Virology at the Institute of Sera and Vaccines, Prague, Czech Republic
  • 1986: Research Associate in the Laboratory of Arboviruses at the Institute of Parasitology, Czechoslovak Academy of Sciences, Ceske Budejovice, the Czech Republic
  • 1987: C.Sc. degree (equivalent to Ph.D.) in microbiology/virology. Thesis entitled "The study of antigenic structures of mumps virus and their possibilities in the practice employment" in the Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, the Czech Republic.

Employment History

  • 1987: Junior research scientist in the Institute of Parasitology, Czechoslovak Academy of Sciences, Ceske Budejovice.
  • 1990-1994: Secretary for Science at the Institute of Parasitology, Czechoslovak Academy of Sciences, Ceske Budejovice.
  • 1991: Appointed Head of the Laboratory of Molecular Ecology of Parasites at the Institute of Parasitology, Czechoslovak Academy of Sciences, Ceske Budejovice.
  • 1993: Appointed assistant Professor in Biochemistry, Faculty of Biological Sciences, the University of South Bohemia, Česke Budějovice, the Czech republic.
  • 1994-2002: Director of the Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceske Budejovice.
  • 1997: Appointed associated Professor in Molecular and Cellular Biology, Faculty of Biological Sciences, the University of South Bohemia, Česke Budějovice. Teaches folowing courses: Biochemistry, Biochemistry and molecular biology of parasites, General Virology.
  • 2001: Appointed Professor in Molecular and Cellular Biology and Genetics
  • 2001-2004: Vice-rector of the University of Southern Bohemia for international relations.
  • 2002-: Chairman of the Scientific Council of the Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceske Budejovice.
  • 2002-: Czech Republic representative in the Central and Eastern European Countries (CEEC) forum at the International Consortium on Ticks and Tick-borne Diseases (ICTTD-2/3).
  • 2003-: Chairman of the Biomedical and molecular biological sciences panel #5 of the Grant Agency of the Academy of Sciences of the Czech Republic.
  • 2003-: Executive chairman of the National Committee of the International Union of Biological Sciences.
  • 2003-: Member of the standing committee on a D.Sc. degree defence in ´Ecological Biology´.
  • 2003-: Vice-chairman of the Committee for evaluation of the institutional research plans and results of institutes of the Academy o Sciences of the Czech Republic in Life sciences and Chemistry.

Scientific Background, Research Experience

Biochemistry and molecular biology of disease vectors; molecular factors in mechanisms of pathogens transmission with the respect to the role of protein-carbohydrate as well as other non-covalent interactions, immune reactions in arthropods. At present, he is concerned with the investigation of structural and functional components of Borrelia burgdorfferi and B. recurrentis spirochetes. He is also interested in tick-borne encephalitis virus, its virulence, separation, purification, isolation of the envelope glycoprotein E, and in the study on structural properties of this antigen. He also deals with the interaction of borreliae and TBE virus with internal environment of ixodid ticks. The other domain of his interest is the immunity of invertebrates and molecular biological factors influencing it. He has worked on the isolation and functional characterization of structures important for physiology of recognition and defense reaction in invertebrates. Till now, he has been a principal investigator of 4 grants of GA ASCR, 3 grants of GA CR, 3 grants of the Ministry of Education, one of those is VS 96066 ("Strengthening of Research at Universities"), and a co-principal investigator of two internatinal projects (IAAE; Fogarty Foundation/N.I.H.). A joint research project with NRC-IBS Ottawa within S&T Cooperation/Canada´(2003-2006).

Long-term Study Stays Abroad and International Collaborations

1989 University of Uppsala (Prof. K. Soderhall), 1992-1993, 1995 University of Arizona (Prof. M. Wells, Prof. J.Law). Develops a tight collaboration with the University of Arizona (within Biomedical Research Abroad: Vistas Open! programme), the University of Glasgow (1997-Prof. I. Maudlin; 2000-Dr. R. A. Smith: work placement of undergraduate students, Socrates/Erasmus), Karel Grote Hogeschol in Antwerpen (student mobility within Erasmus/Socrates, teaching a class on Biochemistry of Parasites). 2003 Georgia Southern University, Statesboro (USA), Institute of Parasitology and Arthropodology, (Prof. J. H. Oliver, Jr.) within NATO Senior Research Fellowship on molecular factors in tick-borrelia interactions.

Published a total of 70 scientific articles, 62 papers at conferences and 16 invited lectures. Member of the editorial board of Folia Parasitologica and of several scientific societies, and scienticic counciles of the University of South Bohemia in České Budějovice (USB), the Institute of Entomology AS CR, Institute of Plant Molecular Biology AS CR, Institute of Landscape Ecology AS CR, the Faculty of Biological Sciences USB, and the Institute of Physical Biology USB.

Teaching Biochemistry
Faculty of Science, University of South Bohemia

Basic Laboratory Course in Biochemistry
Faculty of Science, University of South Bohemia

Biochemistry I
Faculty of Science, University of South Bohemia

Biochemistry 1
Faculty of Science, University of South Bohemia

Biochemistry 2
Faculty of Science, University of South Bohemia

Biological Chemistry - Bachelor Seminar
Faculty of Science, University of South Bohemia

Developmental and Comparative Biochemistry (for Biological Chemistry)
Faculty of Science, University of South Bohemia

Glycobiochemistry (for Biological Chemistry)
Faculty of Science, University of South Bohemia

Laboratory practise from Biochemistry
Faculty of Science, University of South Bohemia

Laboratory Course in Biochemistry
Faculty of Science, University of South Bohemia

Advanced Laboratory Course in Biochemistry
Faculty of Science, University of South Bohemia

Seminar in Advanced Biological Chemistry
Faculty of Science, University of South Bohemia

Seminar in Advanced Biological Chemistry II
Faculty of Science, University of South Bohemia

Publications
Total found: 114 records
de la Fuente J., Antunes S., Bonnet S., Cabezas-Cruz A., Domingos A., Estrada-Peňa A., Johnson N., Kocan K., Mansfield K., Nijhof A., Papa A., Rudenko N., Villar M., Alberdi P., Torina A., Ayllón N., Vancová M., Golovchenko M., Grubhoffer L., Caracappa S., Fooks A., Gortazar C., Rego R. O. M. (2017) Tick-pathogen interactions and vector competence: identification of molecular drivers for tick-borne diseases Frontiers in Cellular and Infection Microbiology 7 : 114.
DOI: 10.3389/fcimb.2017.00114
Selinger M., Wilkie G., Tong L., Gu Q., Schnettler E., Grubhoffer L., Kohl A. (2017) Analysis of tick-borne encephalitis virus-induced host responses in human cells of neuronal origin and interferon-mediated protection Journal of General Virology 98 : 2043-2060.
DOI: 10.1099/jgv.0.000853
Strnad M., Hönig V., Růžek D., Grubhoffer L., Rego R. O. M. (2017) Europe-wide meta-analysis of Borrelia burgdorferi sensu lato prevalence in questing Ixodes ricinus ticks Applied and Environmental Microbiology 83 : e00609-17.
DOI: 10.1128/AEM.00609-17
Vancová M., Rudenko N., Vaněček J., Golovchenko M., Strnad M., Rego R. O. M., Tichá L., Grubhoffer L., Nebesářová J. (2017) Pleomorphism and viability of the Lyme disease pathogen Borrelia burgdorferi exposed to physiological stress conditions: a correlative cryo-fluorescence and cryo-scanning electron microscopy study Frontiers in Microbiology 8 : 596.
DOI: 10.3389/fmicb.2017.00596
Cabezas-Cruz A., Zweygarth E., Vancová M., Broniszewska M., Grubhoffer L., Passos L., Ribeiro M., Alberdi P., de (2016) Ehrlichia minasensis sp. nov., a new species within the genus Ehrlichia isolated from the tick Rhipicephalus microplus International Journal of Systematic and Evolutionary Microbiology 66 : 1426-1430.
DOI: 10.1099/ijsem.0.000895
Černý J., Selinger M., Palus M., Vavrušková Z., Tykalová H., Bell-Sakyi L., Štěrba J., Grubhoffer L., Růžek D. (2016) Expression of a second open reading frame present in the genome of tick-borne encephalitis virus strain Neudoerfl is not detectable in infected cells Virus Genes 52 : 309-316.
DOI: 10.1007/s11262-015-1273-y
Golovchenko M., Vancová M., Clark K., Oliver J., Grubhoffer L., Rudenko N. (2016) A divergent spirochete strain isolated from a resident of the southeastern United States was identified by multilocus sequence typing as Borrelia bissettii Parasites & Vectors 9 : 68.
DOI: 10.1186/s13071-016-1353-4
Rudenko N., Golovchenko M., Clark K., Oliver J., Grubhoffer L. (2016) Detection of Borrelia burgdorferi sensu stricto in Amblyomma americanum ticks in the southeastern United States: the case of selective compatibility. Emerging Microbes and Infection 5 : e48.
DOI: 10.1038/emi.2016.45
Rudenko N., Golovchenko M., Vancová M., Clark K., Grubhoffer L., Oliver J. (2016) Isolation of live Borrelia burgdorferi sensu lato spirochetes from patients with undefined disorders and symptoms not typical for Lyme diseases Clinical Microbiology and Infection 22 : 267.e9-267.e15.
DOI: 10.1016/j.cmi.2015.11.009
Tichá L., Golovchenko M., Oliver J., Grubhoffer L., Rudenko N. (2016) Sensitivity of Lyme Borreliosis Spirochetes to Serum Complement of Regular Zoo Animals: Potential Reservoir Competence of Some Exotic Vertebrates Vector-Borne and Zoonotic Diseases 16 : 13-19.
DOI: 10.1089/vbz.2015.1847
Černý J., Černá Bolfíková B., De A. Zanotto P., Grubhoffer L., Růžek D. (2015) A deep phylogeny of viral and cellular right-hand polymerases Infection, Genetics and Evolution 36 : 275-286.
DOI: 10.1016/j.meegid.2015.09.026
Elsterová J., Černý J., Müllerová J., Šíma R., Coulson S., Lorentzen E., Strom H., Grubhoffer L. (2015) Search for tick-borne pathogens in the Svalbard archipelago and Jan Mayen Polar Research 34 : 27466.
DOI: 10.3402/polar.v34.27466
Hönig V., Švec P., Halas P., Vavrušková Z., Šťastná H., Kilian P., Vrtišková V., Dorňáková V., Dupejová J., Simonová Z., Mondeková H., Erhart J., Štěrba J., Golovchenko M., Grubhoffer L., Rudenko N. (2015) Ticks and tick-borne pathogens in South Bohemia (Czech Republic) - spatial variability in Ixodes ricinus abundance, Borrelia burgdorferi and tick-borne encephalitis virus prevalence Ticks and Tick-borne diseases 6 : 559-567.
DOI: 10.1016/j.ttbdis.2015.04.010
Strnad M., Elsterová J., Schrenková J., Vancová M., Rego R. O. M., Grubhoffer L., Nebesářová J. (2015) Correlative cryo-fluorescence and cryo-scanning electron microscopy as a straightforward tool to study host-pathogen interactions Scientific Reports 5 : 18029.
DOI: 10.1038/srep18029
Tonk M., Cabezas-Cruz A., Valdés J. J., Grubhoffer L., Rego R. O. M., Estrada-Peňa A., Vilcinskas A., Kotsyfakis M., Rahnamaeian M. (2015) Ixodes ricinus defensins attack distantly-related pathogens Developmental & Comparative Immunology 53 : 358-365.
DOI: 10.1016/j.dci.2015.08.001
Villar M., Ayllón N., Kocan K., Bonzón-Kulichenko E., Alberdi P., Blouin E., Weisheit S., Mateos-Hernández L., Cabezas-Cruz A., Bell-Sakyi L., Vancová M., Bílý T., Meyer D., Štěrba J., Contreras M., Rudenko N., Grubhoffer L., Vázquez J., de la Fuente J. (2015) Identification and Characterization of Anaplasma phagocytophilum Proteins Involved in Infection of the Tick Vector, Ixodes scapularis PLoS ONE 10 : e0137237.
DOI: 10.1371/journal.pone.0137237
Weisheit S., Villar M., Tykalová H., Popara M., Loecherbach J., Watson M., Růžek D., Grubhoffer L., de la , Fazakerley J., Bell-Sakyi L. (2015) Ixodes scapularis and Ixodes ricinus tick cell lines respond to infection with tick-borne encephalitis virus: transcriptomic and proteomic analysis Parasites & Vectors 8 : 599.
DOI: 10.1186/s13071-015-1210-x
Černý J., Černá Bolfíková B., Valdés J. J., Grubhoffer L., Růžek D. (2014) Evolution of Tertiary Structure of Viral RNA Dependent Polymerases PLoS ONE 9 : e96070.
DOI: 10.1371/journal.pone.0096070
Chrudimská T., Čeřovský V., Slaninová J., Rego R. O. M., Grubhoffer L. (2014) Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 46 : 165-170.
DOI: 10.1016/j.dci.2014.04.005
Crowder C., Carolan H., Rounds M., Honig V., Mothes B., Haag H., Nolte O., Luft B., Grubhoffer L., Ecker D., Schutzer S., Eshoo M. (2014) Prevalence of Borrelia miyamotoi in Ixodes Ticks in Europe and the United States Emerging Infectious Diseases 20 : 1678-1682.
DOI: 10.3201/eid2010.131583

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CONTACT

Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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