RNDr. Ján Štěrba, Ph.D.

Job Position: PostDoc - Laboratory of Molecular Ecology of Vectors and Pathogens

Contact details

Phone: +420 387776220
E-mail: sterbaj@paru.cas.cz
Room: PrF C 01 008

Curriculum vitae

Postdoctoral research fellow

Institute of Parasitology

Biology Centre of the Academy of Sciences of the Czech Republic

&

Assistant professor, Deputy head

Institute of Chemistry and Biochemistry

Faculty of Science, University of South Bohemia

Research interests:

Glycobiology of ticks and tick-borne pathogens, allergenogenic arthropod glycans, mass spectrometry, bioorthogonal chemistry

Education and employment:

2002: BSc. in Chemistry, Faculty of Sciences, University of P. J. Šafárik, Košice, Slovak Republic

2003: studies at Laboratory of Biochemistry, Faculty of Chemistry, Aristoteles University in Thessaloniki within Socrates-Erasmus exchange program

2004: graduated with honors from chemistry (specialization biochemistry), Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, University of P. J. Šafárik, Košice, Slovak Republic; award of the dean of Faculty of Natural Sciences, University of P. J. Šafárik.

2004: Laboratory of Clinical Biochemistry, Health Centre Veľké Kapušany, Veľké Kapušany, Slovak Republic

2004-2012: Ph.D. studies in Molecular and Cellular Biology and Genetics at the Faculty of Biological Sciences (now Faculty of Science), University of South Bohemia, České Budějovice, Czech Republic

2004-2006: research assistant, Biology Centre of Academy of Sciences of the Czech Republic v.v.i., Institute of Parasitology, České Budějovice, Czech Republic

2006: assistant at Faculty of Biological Sciences, USB, České Budějovice

2006: visiting scientist, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada 

2007-: assistant professor at Faculty of Biological Sciences, USB, České Budějovice; now Faculty of Science, USB, České Budějovice

2007: visiting scientist, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada 

2009, 2010: visiting scientist, National Center for Glycomics and Glycoproteomics, Department of Chemistry, Indiana University, Bloomington, Indiana, USA

2010-: postdoctoral research fellow, Institute of Parasitology, Biology Centre of ASCR, České Budějovice

2014/2015: research stay, Genomics group, SaBio (Sanidad y Biotecnología) research group, National Wildlife Research Institute IREC (CSIC-Universidad de Castilla – La Mancha), Ciudad Real, Spain.

Teaching(at Faculty of Science, University of South Bohemia):

Pharmacology and Toxicology (lectures + practical course, in Czech) and Introduction to Toxicology (lectures + practical course, in Czech);

Xenobiochemistry and Toxicology (lectures + practical course, in English);

Laboratory Course in Biochemistry (in Czech and in English), and Adnavced Course in Bicohemistry (in Czech and in English);

supervisor and co-supervisor of 20 bachelor and masters students, co-supervisor of a student from University of Glasgow (work placement at Faculty of Science, University of South Bohemia)

Published scientific papers:

Růžek D., Kopecký J., Štěrba J., Golovchenko M., Rudenko N., Grubhoffer L.: Non-virulent strains of TBE virus circulating in the Czech Republic. Journal of Clinical Virology 36 (suppl. 2): S41, 2006 (P117).

Růžek D., Štěrba J., Kopecký J., Grubhoffer L.: The supposedly attenuated Hy-HK variant of highly virulent Hypr strain of tick-borne encephalitis virus is obviously a stain of Langat virus. Acta Virologica 50(4), 227-278, 2006.

Štěrba J., Vancová M., Rudenko N., Golovchenko M., Tremblay T.-L., Kelly J.F., MacKenzie R.C., Logan S.M., Grubhoffer L.: Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated. Journal of Bacteriology 190: 2619-2623, 2008.

Man P., Kovář V., Štěrba J., Strohalm M., Kavan D., Kopáček P., Grubhoffer L., Havlíček V.: Deciphering Dorin M glycosylation by mass spectrometry. European Journal of Mass Spectrometry 14: 345-354, 2008.

Štěrba J., Dupejová J., Fišer M., Golovchenko M., Rudenko N., Grubhoffer L.: Identification and characterisation of lectins in several tick species. FEBS Journal, 276 (Suppl. 1): 88, 2009.

Grubhoffer L., Hajdušek O., Vancová M., Štěrba J., Rudenko N.: Glycobiochemistry of ticks, vectors of infectious diseases: carbohydrate-binding proteins and glycans. FEBS Journal, 276 (Suppl. 1): 141, 2009.

Dupejova J., Sterba J., Vancova M., Grubhoffer L.: Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization. Parasites&Vectors, 4:4, 2011.

Sterba J., Dupejova J., Fiser M., Vancova M., Grubhoffer L.: Fibrinogen-related proteins in ixodid ticks. Parasites&Vectors, 4:127, 2011.

Vancova M., Sterba J., Dupejova J., Simonova Z., Nebesarova J., Novotny M.V., Grubhoffer L.: Uptake and incorporation of sialic acid by the tick Ixodes ricinus. Journal of Insect Physiology, 2012, 58, 1277-1287.

Grubhoffer L., Rudenko N., Vancova M., Golovchenko M., Sterba J.: Circulatory system and hemolymph. In: Biology of ticks. Vol. 1. Daniel E. Sonenshine (Editor), R. Michael Roe (Editor).  Oxford University Press Inc; 2013, 2nd Revised edition. pp. 258-286.

Sterba J., Vancova M., Sterbova J., Bell-Sakyi L., Grubhoffer L.: The majority of sialylated glycoproteins in adult Ixodes ricinus ticks originate in the host, not the tick. Carbohydr Res, 2014, 389, 93-99.

Hönig V, Svec P, Halas P, Vavruskova Z, Tykalova H, Kilian P, Vetiskova V, Dornakova V, Sterbova J, Simonova Z, Erhart J, Sterba J, Golovchenko M, Rudenko N, Grubhoffer L.: Ticks and tick-borne pathogens in South Bohemia (Czech Republic) - Spatial variability in Ixodes ricinus abundance, Borrelia burgdorferi and tick-borne encephalitis virus prevalence. Ticks Tick Borne Dis,  2015, in press.

Attended conferences:

more than 25 poster presentations and 6 lectures on international conferences

Projects:

2006: Glycosylation of tick-borne encephalitis virus envelope protein and its influence on virus virulence – Grantová agentura JU (PI),

2007: Glycosylation of membrane proteins of Lyme borreliosis spirochetes – Grantová agentura JU (PI),

2009-2011: Glycoprotein analysis of Ixodes ricinus salivary glands – GA AVČR (PI: Dr. Marie Vancová, Institute of Parasitology, BC ASCR, České Budějovice, co-PI: Ján Štěrba, Faculty of Science, University of South Bohemia, České Budějovice),

2016-2022: Research infrastructure C4SYS – MŠMT (PI: R. Ettrich, J. Štěrba – head of one of the research units, member of the Steering Committee).

Team member:

2008-2010: Molecular pathogenesis of tick-borne encephalitis – GAČR,

2009-2011: Interaction of Lyme borreliosis spirochetes and salivary glands of Ixodes ricinus tick: the role of borrelia OspC protein – GAČR,

2005-2011: Centre of molecular ecology of vectors and pathogens – MŠMT,

2010: Distribution and diversity of B. bissettii in the southeastern USA and in Europe – U. S. Civilian Research & Development Foundation,

2015-2017: Novel functions of viral and cellular proteins in tick-borne encephalitis virus infection – GAČR.

Publications
Total found: 13 records
Černý J., Selinger M., Palus M., Vavrušková Z., Tykalová H., Bell-Sakyi L., Štěrba J., Grubhoffer L., Růžek D. (2016) Expression of a second open reading frame present in the genome of tick-borne encephalitis virus strain Neudoerfl is not detectable in infected cells Virus Genes 52 : 309-316.
DOI: 10.1007/s11262-015-1273-y
Dzyuba V., Słowińska M., Cosson J., Ciereszko A., Boryshpolets S., Štěrba J., Rodina M., Linhart O., Dzyuba B. (2016) Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation Fish Physiology and Biochemistry 42 : 1755-1766.
DOI: 10.1007/s10695-016-0255-x
Shaliutina-Kolešová A., Kotas P., Štěrba J., Rodina M., Dzyuba B., Cosson J., Linhart O. (2016) Protein Profile of Seminal Plasma and Functionality of Spermatozoa During the Reproductive Season in the Common Carp (Cyprinus carpio) and Rainbow trout (Oncorhynchus mykiss) Molecular Reproduction and Development 83 : 968-982.
DOI: 10.1002/mrd.22737
Hönig V., Švec P., Halas P., Vavrušková Z., Šťastná H., Kilian P., Vrtišková V., Dorňáková V., Dupejová J., Simonová Z., Mondeková H., Erhart J., Štěrba J., Golovchenko M., Grubhoffer L., Rudenko N. (2015) Ticks and tick-borne pathogens in South Bohemia (Czech Republic) - spatial variability in Ixodes ricinus abundance, Borrelia burgdorferi and tick-borne encephalitis virus prevalence Ticks and Tick-borne diseases 6 : 559-567.
DOI: 10.1016/j.ttbdis.2015.04.010
Villar M., Ayllón N., Kocan K., Bonzón-Kulichenko E., Alberdi P., Blouin E., Weisheit S., Mateos-Hernández L., Cabezas-Cruz A., Bell-Sakyi L., Vancová M., Bílý T., Meyer D., Štěrba J., Contreras M., Rudenko N., Grubhoffer L., Vázquez J., de la Fuente J. (2015) Identification and Characterization of Anaplasma phagocytophilum Proteins Involved in Infection of the Tick Vector, Ixodes scapularis PLoS ONE 10 : e0137237.
DOI: 10.1371/journal.pone.0137237
Štěrba J., Vancová M., Dupejová J., Bell-Sakyi L., Grubhoffer L. (2014) The majority of sialylated glycoproteins in adult Ixodes ricinus ticks originate in the host, not the tick Carbohydrate Research 389 : 93-99.
DOI: 10.1016/j.carres.2014.02.017
Vancová M., Štěrba J., Dupejová J., Simonová Z., Nebesářová J., Novotný M., Grubhoffer L. (2012) Uptake and incorporation of sialic acid by the tick Ixodes ricinus. Journal of Insect Physiology 58 : 1277–1287.
DOI: 10.1016/j.jinsphys.2012.06.016
Dupejová J., Štěrba J., Vancová M., Grubhoffer L. (2011) Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization. Parasites & Vectors 4 : art. no. 4.
DOI: 10.1186/1756-3305-4-4
Štěrba J., Dupejová J., Fišer M., Vancová M., Grubhoffer L. (2011) Fibrinogen-related proteins in ixodid ticks. Parasites & Vectors 4 : e127.
DOI: 10.1186/1756-3305-4-127
Man P., Kovář V., Štěrba J., Strohalm M., Kavan D., Kopáček P., Grubhoffer L., Havlíček V. (2008) Deciphering Dorin M glycosylation by mass spectrometry. European Journal of Mass Spectrometry 14 : 345-354.
Štěrba J., Vancová M., Rudenko N., Golovchenko M., Tremblay T., Kelly J., Mackenzie R., Logan S., Grubhoffer L. (2008) Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated. Journal of Bacteriology 190 : 2619-2623.
DOI: 10.1128/JB.01885-07
Růžek D., Štěrba J., Kopecký J., Grubhoffer L. (2006) The supposedly attenuated Hy-HK variant of highly virulent Hypr strain of tick-borne encephalitis virus is obviously a stain of Langat virus. Acta Virologica 50 : 277-278.
Starý P., Štěrba J., Soldán T. (1988) Perilitus areolaris G. & H. (Hymenoptera, Braconidae), a parasitoid of the large pine weevil Hylobius abietis (L.) in Czechoslovakia. Acta ent. bohemoslov. 85 : 428-433.

CONTACT

Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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