Dr. Michail Kotsyfakis, Ph.D.

Job Position: Head of Laboratory - Laboratory of Genomics and Proteomics of Disease Vectors

Contact details

Phone: +420 387775492
E-mail: kotsyfakis@paru.cas.cz
Room: 232

Curriculum vitae

Employment history
 
09/2009 – present: Principal Investigator at the Laboratory of Genomics and Proteomics of Disease Vectors, Biology Centre, Czech Academy of Sciences, Czech Republic.   
 
3/2005 - 8/2009: Post-doctoral research associate at the Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA.
Mentor: Professor José M. C. Ribeiro
 
12/2004 - 2/2005: Post-doctoral fellow at the Laboratory of Insect Molecular Genetics, Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology - Hellas (F.O.R.T.H.), Heraklion Crete, Greece.
Mentor: Professor Christos (Kitsos) Louis
 
Academic record
 
11/2000 - 11/2004: Ph.D. in Biology, Joint Graduate Program of the Departments of Medicine and Biology, University of Crete, and the Institute of Molecular Biology and Biotechnology, F.O.R.T.H., Heraklion Crete, Greece.
 
Thesis title: A study of the Anopheles annexin family as proteins that bind Plasmodium berghei parasites 
(2004, University of Crete)
 
Ph.D. Advisor: Professor Christos (Kitsos) Louis
 
11/1998 - 11/2000: M.Sc. in Molecular Biology and Biomedicine, Joint Graduate Program of the Departments of Medicine and Biology, University of Crete, and the Institute of Molecular Biology and Biotechnology, F.O.R.T.H., Heraklion Crete, Greece.
 
Thesis title: Studies on the interaction of Plasmodium parasites with Anopheles mosquitoes 
(2000, University of Crete)
 
M.Sc. Advisor: Professor Christos (Kitsos) Louis
 
10/1994 - 11/1998: B.Sc. in Biology, University of Crete, Heraklion Crete, Greece.
 
 
 
 
Grants (since group establishment in 2009)
 
 
 

 

 

 

Agency & grant type

 

 

 

Coordinator name

 

 

 

Coordinator’s laboratory

 

 

 

Partner’s

Name & lab

 

 

 

Duration

 

 20xx-201y

Amount in k€

Team budget (Total to the consortium)

EU FP7 transnational facility access program TRANSVAC, Project acronym DUALVAC-ANTIRICINUS

 

 

Michail Kotsyfakis

 

Laboratory of Genomics and Proteomics of Disease Vectors

 

 

None

 

 

2010-2011

 

 

50

Marie Curie EU FP7 Reintegration grant, Grant agreement PIRG07-GA-2010-268177

 

 

Michail Kotsyfakis

 

Laboratory of Genomics and Proteomics of Disease Vectors

 

 

None

 

 

2010-2014

 

 

100

 

National Institutes of Health, USA, R01 grant, Grant Number 1R01AI093653-01A1

 

 

Joao Pedra

Department of Microbiology and Immunology

University of Maryland School of Medicine

 

 

Michail Kotsyfakis

 

 

2011-2016

 

 

100 (1000)

Grant Agency of Czech Republic, Grant number: P502/12/2409

 

Michail Kotsyfakis

Laboratory of Genomics and Proteomics of Disease Vectors

 

 

None

 

 

2012-2015

 

 

360

FP7-specific program Capacities, THEME: Research Potential, REGPOT-2008-1, Call identifier: FP7-REGPOT-2012-2013-1, Project acronym: MODBIOLIN,Grant agreement No.: 316304

 

Biology Centre of the Academy of Sciences of the Czech Republic

 

 

 

 

Biology Centre of the Academy of Sciences of the Czech Republic

 

 

 

14 principal investigators at the Biology Centre receive benefits from the actions of the program

 

 

 

 

2013-2015

 

 

 

 

(3,433)

 
 
 
Selected oral presentations/talks at international conferences 
(last 3 years, 34 throughout entire career)
 
 
- 10th European Congress of Entomology, 3-8 August 2014, York, United Kingdom (invited speaker).
- National Institute on Aging, National Institutes of Health, 19 May 2014, Baltimore, USA (invited seminar).
- 8th General Meeting of the International Proteolysis Society, 20-24 October 2013, Cape Town, South Africa.
- EMBO Conference: Molecular and Population Biology of Mosquitoes and Other Disease Vectors: From Basic Vector Biology to Disease Control, 15-19 July 2013, Kolymbari, Crete, Greece.
- 5th International Conference on Exogenous Factors Affecting Thrombosis and Haemostasis, 5-6 July 2013, Amsterdam, The Netherlands (invited speaker). 
- 24th International Congress of Entomology, 19-24 August 2012, Daegu, South Korea (invited speaker). 
- University of California-Riverside, Department of Entomology, Center for Disease Vector Research, 12 May 2012, Riverside, California, USA (invited seminar).
- Seventh International Conference on Ticks and Tick-borne Pathogens, 28 August-2 September 2011, Zaragoza, Spain (co-chair of a session).
- Fifth International Meeting on Molecular and Population Biology of Mosquitoes and Other Disease Vectors, 24-30 July 2011, Kolymbari, Crete, Greece (co-chair of session). 
 
 
On-going collaborations (alphabetically)
 
 
Bruno Arca, Professor at the Dipartimento di Scienze di Sanità Pubblica, Università “La Sapienza”, Rome, Italy.
Triantafyllos Chavakis, Professor and Division Chief, University Clinic Carl Gustav Carus, Dresden University of Technology, Dresden, Germany.
Michael Hackenberg, Assistant Professor, Dpto. de Genética, Facultad de Ciencias, Campus Fuentenueva s/n, Universidad de Granada, Granada, Spain.
Shahid Karim, Associate Professor at the Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, Mississippi, USA.
Joao H Pedra, Assistant Professor at the Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, USA.
José MC Ribeiro, Head of the Vector Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, USA.
Edgar Schmitt, Professor, Institute of Immunology, Johannes Gutenberg University of Mainz, Mainz, Germany.
Stefan Tenzer, Head of Mass-Spec Core Facility, Johannes Gutenberg University of Mainz, Mainz, Germany.
 
 
Fellowships / Research abroad
 
10/2013: EMBO laboratory management course for group leaders, Leimen, Germany.
8/2013: Research stay in the Department of Internal Medicine, University Clinic Carl Gustav Carus, Dresden University of Technology, Dresden, Germany (Laboratory head: Professor Triantafyllos Chavakis).
3/2013 - 4/2013, 9/2013: EMBO Short-Term Fellowship (ASTF number 482-2012). 7,200 Euros in total. This fellowship supported a visit as a Special Volunteer to the Laboratory of Malaria & Vector Research, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Rockville, USA (Laboratory head: Professor Jose' MC Ribeiro).  
2/2010 - 2/2012: Alexander von Humboldt fellowship to Dr Alexandra Schwarz to join my group as a post-doctoral fellow (host laboratory). 26,000 Euros per year, 52,000 Euros in total. This fellowship supported the stay of Dr. Schwarz as a post-doctoral fellow in my laboratory at the Biology Centre, Academy of Sciences of the Czech Republic, Budweis, Czech Republic.
9/2009 - 8/2014: Jan Evangelista Purkyně Fellowship from the Czech Academy of Sciences (the first to receive the fellowship in South Bohemia and the first non-Czech speaker). 30,000 Euros per year, 150,000 Euros in total. The fellowship supported my salary as a group leader at the Biology Centre, Czech Academy of Sciences, Budweis, Czech Republic.
3/2005 - 10/2009: Post-doctoral Visiting Fellowship, Laboratory of Malaria & Vector Research, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Rockville, USA. (Intramural grant number 0012117069). 45,000 US dollars per year, ~ 200,000 US dollars in total.
6/2002 - 8/2002: EMBO Short-Term Fellowship (ASTF number 67-2002). The fellowship supported my stay as a graduate student in the Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France under the directorship of the Nobel laureate Professor Jules Hoffmann.
 
 
Memberships and awards
 
10/2005 - present: International Proteolysis Society Member.
1/2008: American Society for Biochemistry and Molecular Biology Post-doctoral Travel Award to participate in the 2008 society meeting in San Diego, USA.
11/2007 - present: American Society for Biochemistry and Molecular Biology Member (ID: 23561).
9/2007: NIH Fellows Award for Research Excellence in Biomedical Research.
8/2007: International Proteolysis Society (IPS) travel award to participate in the 5th IPS meeting in Patras, Greece.
1/2007: Young Scientist Award to participate in the 10th International Union of Biochemistry and Molecular Biology/Sociedade Brasileira de Bioquimica e Biologia (IUBMB/SBBq) meeting in Salvador, Brazil.
9/2006: NIH Fellows Award for Research Excellence in Biomedical Research.
8/2005: International Proteolysis Society travel award to participate in the 4th IPS meeting in Quebec, Canada.
 
Scientific reviewer 
 
- Associate editor for BMC Genomics and the American Journal of Blood Research. 
- External grant reviewer for France’s Agence Nationale de la Recherche (ANR), the Swiss Secretariat for Education, Research and Innovation (SERI), the National Science Centre of Poland (NCN), and the Slovak Research and Development Agency (APVV).
- External reviewer for: Blood, PNAS, Journal of Proteome Research, PLoS Neglected Tropical Diseases, BMC Genomics, Journal of Proteomics, The FEBS Journal, FEBS Letters, Developmental & Comparative Immunology, PLoS One, Insect Biochemistry and Molecular Biology, International Journal for Parasitology, Parasites and Vectors, Biochimie, Ticks and Tick-borne Diseases, Gene, Veterinary Parasitology, Pesticide Biochemistry and Physiology, Sensors, Physiological Entomology.
 
Teaching and supervisory experience 
 
10/2012 - present: Undergraduate supervision (Ms. Lovelyna Eromonsele), Faculty of Science, University of South Bohemia, Budweis, Czech Republic.
10/2011 - 1/2014: M.Sc. supervision (Mr. Jan Kotal), Faculty of Science, University of South Bohemia, Budweis, Czech Republic.
09/2010 - 01/2011: Teaching Molecular and Biochemical Parasitology, Faculty of Science, University of South Bohemia, Budweis, Czech Republic (affiliation with Faculty of Science, University of South Bohemia, Budweis, Czech Republic). 
10/2009 - 6/2011: Undergraduate supervision (Ms. Barbora Singerova and Mr. Jan Kotal) at the Faculty of Science, University of South Bohemia, Budweis, Czech Republic.

                     

Previous PhD students

Name

Start Date

Defence Date

Jindrich Chmelar (co-supervisor)

January 2008

November 2010

Helena Horka (co-supervisor)

January 2009

May 2011

Veronika Dornakova (co-supervisor)

January 2011

Found a job

 

Current PhD  student

 

PhD student Name

Start Date

Planned End Date

 
 

Jan Kotal

February 2014

June 2017

 

 

Post-Doc mentoring

 

Post-Doc Name

Year of PhD

Start Date

End Date

 

 
 

Jindrich Chmelar

2010

December 2010

December 2011

 

Alexandra Schwarz

2009

February 2010

February 2014

 

James Valdes

2009

August 2011

September 2012

 

 
 
Honours 
 
● I was awarded the NIH Fellows Award for Research Excellence in Biomedical Research for two consecutive years, which places me in the top 10% of NIH intramural research fellows. I have twice been supported by an EMBO-Short-Term fellowship and selected to attend the EMBO Laboratory management course for group leaders.
● I was the first non-Czechoslovakian honoured with a Jan Evangelista Purkyně Fellowship, established by the Czech Academy of Sciences to facilitate the recruitment and integration of recognised senior investigators in the national research infrastructure. This was the first fellowship awarded to an individual at a research institution outside Prague and to a non-Czech speaker, highlighting the recognition by the Czech academia of my scientific contribution to tick-borne diseases. 

Teaching The Human Plasma Proteome
Faculty of Science, University of South Bohemia

Publications
Total found: 47 records
Chmelař J., Kotál J., Langhansová H., Kotsyfakis M. (2017) Protease inhibitors in tick saliva: the role of serpins and cystatins in tick-host-pathogen interaction Frontiers in Cellular and Infection Microbiology 7 : 216.
DOI: 10.3389/fcimb.2017.00216
Hackenberg M., Langenberger D., Schwarz A., Erhart J., Kotsyfakis M. (2017) In silico target network analysis of de novo-discovered, tick saliva-specific microRNAs reveals important combinatorial effects in their interference with vertebrate host physiology RNA 23 : 1259-1269.
DOI: 10.1261/rna.061168
Shaw D., McClure E.E., Kotsyfakis M., Pedra J. (2017) Tick saliva and microbial effector molecules: two sides of the same coin Arthropod Vector: Controller of Disease Transmission (Volume 2): Vector Saliva-Host-Pathogen Interactions, S.K. Wikel, S. Aksoy and G. Dimopoulos (Eds.) 169-194.
DOI: 10.1016/B978-0-12-805360-7.00010-1
Assumpção T., Ma D., Mizurini D., Kini R., Ribeiro J., Kotsyfakis M., Monteiro R., Francischetti I. (2016) In Vitro Mode of Action and Anti-thrombotic Activity of Boophilin, a Multifunctional Kunitz Protease Inhibitor from the Midgut of a Tick Vector of Babesiosis, Rhipicephalus microplus PLoS Neglected Tropical Diseases 10 : e0004298.
DOI: 10.1371/journal.pntd.0004298
Cabezas-Cruz A., Tonk M., Bouchut A., Pierrot C., Pierce R., Kotsyfakis M., Rahnamaeian M., Vilcinskas A., Khalife J., Valdés J. J. (2016) Antiplasmodial activity is an ancient and conserved feature of tick defensins Frontiers in Microbiology 7 : 1682.
DOI: 10.3389/fmicb.2016.01682
Chmelař J., Kotál J., Karim S., Kopáček P., Francischetti I., Pedra J., Kotsyfakis M. (2016) Sialomes and Mialomes: A Systems-Biology View of Tick Tissues and Tick-Host Interactions Trends in Parasitology 32 : 242-254.
DOI: 10.1016/j.pt.2015.10.002
Chmelař J., Kotál J., Kopecký J., Pedra J., Kotsyfakis M. (2016) All for one and one for all on the tick–host battlefield Trends in Parasitology 32 : 368-377.
DOI: 10.1016/j.pt.2016.01.004
Ferreira V., Vale V., Pangburn M., Abdeladhim M., Mendes-Sousa A., Coutinho-Abreu I., Rasouli M., Brandt E., Meneses C., Lima III K., Araújo R., Pereira M., Kotsyfakis M., Oliveira F., Kamhawi S., Ribeiro J., Gontijo N., Collin N., Valenzuela J.G. (2016) SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis Scientific Reports 6 : 19300.
DOI: 10.1038/srep19300
Shaw D., Kotsyfakis M., Pedra J. (2016) For Whom the Bell Tolls (and Nods): Spit-acular Saliva Current Tropical Medicine Reports 3 : 40-50.
DOI: 10.1007/s40475-016-0072-4
Wang X., Shaw D., Hammond H., Sutterwala F., Rayamajhi M., Shirey K., Perkins D., Bonventre J., Velayutham T., Evans S., Rodino K., VieBrock L., Scanlon K., Carbonetti N., Carlyon J., Miao E., McBride J., Kotsyfakis M., Pedra J. (2016) The prostaglandin E2-EP3 receptor axis regulates Anaplasma phagocytophilum-mediated NLRC4 inflammasome activation PLoS Pathogens 12 : e1005803.
DOI: 10.1371/journal.ppat.1005803
Wang X., Shaw D., Sakhon O., Snyder G., Sundberg E., Santambrogio L., Sutterwala F., Dumler J., Shirey K., Perkins D., Richard K., Chagas A., Calvo E., Kopecký J., Kotsyfakis M., Pedra J. (2016) The tick protein sialostatin L2 binds to annexin A2 and inhibits NLRC4-mediated inflammasome activation Infection and Immunity 84 : 1796-1805.
DOI: 10.1128/IAI.01526-15
Jablonka W., Kotsyfakis M., Mizurini D., Monteiro R., Lukszo J., Drake S., Ribeiro J., Andersen J. (2015) Identification and Mechanistic Analysis of a Novel Tick-Derived Inhibitor of Thrombin PLoS One 10 : e0133991.
DOI: 10.1371/journal.pone.0133991
Klein M., Brühl T., Staudt V., Reuter S., Grebe N., Gerlitzki B., Hoffmann M., Bohn T., Ulges A., Stergiou N., De Graaf J., Löwer M., Taube C., Becker M., Hain T., Dietzen S., Stassen M., Huber M., Lohoff M., Campos Chagas A., Andersen J., Kotál J., Langhansová H., Kopecký J., Schild H., Kotsyfakis M., Schmitt E., Bopp T. (2015) Tick Salivary Sialostatin L Represses the Initiation of Immune Responses by Targeting IRF4-Dependent Transcription in Murine Mast Cells The Journal of Immunology 195 : 621-631.
DOI: 10.4049/jimmunol.1401823
Kotál J., Langhansová H., Lieskovská J., Andersen J., Francischetti I., Chavakis T., Kopecký J., Pedra J., Kotsyfakis M., Chmelař J. (2015) Modulation of host immunity by tick saliva Journal of Proteomics 128 : 58-68.
DOI: 10.1016/j.jprot.2015.07.005
Kotsyfakis M., Kopáček P., Franta Z., Pedra J., Ribeiro J. (2015) Deep Sequencing Analysis of the Ixodes ricinus Haemocytome PLOS Neglected Tropical Diseases 9 : e0003754.
DOI: 10.1371/journal.pntd.0003754
Kotsyfakis M., Schwarz A., Erhart J., Ribeiro J. (2015) Tissue- and time-dependent transcription in Ixodes ricinus salivary glands and midguts when blood feeding on the vertebrate host Scientific reports 5 : 9103.
DOI: 10.1038/srep09103
Lieskovská J., Páleníková J., Langhansová H., Chagas A., Calvo E., Kotsyfakis M., Kopecký J. (2015) Tick sialostatins L and L2 differentially influence dendritic cell responses to Borrelia spirochetes Parasites & Vectors 8 : 275.
DOI: 10.1186/s13071-015-0887-1
Lieskovská J., Páleníková J., Širmarová J., Elsterová J., Kotsyfakis M., Campos-Chagas A., Calvo E., Růžek D., Kopecký J. (2015) Tick salivary cystatin sialostatin L2 suppresses IFN responses in mouse dendritic cells Parasite immunology 37 : 70-78.
DOI: 10.1111/pim.12162
Páleníková J., Lieskovská J., Langhansová H., Kotsyfakis M., Chmelař J., Kopecký J. (2015) Ixodes ricinus Salivary Serpin IRS-2 Affects Th17 Differentiation via Inhibition of the Interleukin-6/STAT-3 Signaling Pathway Infection and Immunity 83 : 1949-1956.
DOI: 10.1128/IAI.03065-14
Tonk M., Cabezas-Cruz A., Valdés J. J., Grubhoffer L., Rego R. O. M., Estrada-Peňa A., Vilcinskas A., Kotsyfakis M., Rahnamaeian M. (2015) Ixodes ricinus defensins attack distantly-related pathogens Developmental & Comparative Immunology 53 : 358-365.
DOI: 10.1016/j.dci.2015.08.001

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Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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