Mgr. Jindřich Chmelař, Ph.D.

Job Position: Research Scientist - Laboratory of Genomics and Proteomics of Disease Vectors

Contact details

Phone: 420387775491
E-mail: chmelar@paru.cas.cz

Curriculum vitae

 

Since 1.9. 2005: Ph.D. student at the Department of Parasitology, Faculty of Sciences, University of South Bohemia. The topic of my thesis was the description of the salivary transcripts from the tick Ixodes ricinus and the characterization of salivary serine protese inhibitors.
1. project: An insight into the sialome of Ixodes ricinus
2. project: Characterization of salivary serpin IRS-2 (I. ricinus serpin-2)

EDUCATION

1997: Finished high school, Gymnasium in Český Brod
1997 – 2001: Undergraduate studies in biology at the Faculty of Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic. Specialization in the phylogenetics of parasites. Finished with Bc. degree in biology in February 2002.
2003 – 2005: M.Sc. studies in parasitology at the Faculty of Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic. Specialization in the molecular biology and proteomics of ticks. Finished with Mgr. (M.Sc. equivalent) degree in parasitology.
2008: Succesful defense of RNDr. thesis on the Department of Parasitology, Faculty of Sciences of the University of South Bohemia with thesis „An Insight into the sialome of the castor bean tick, Ixodes ricinus“.

PROFESSIONAL EXPERIENCE

June 2005: Summer school on molecular vector biology, České Budějovice
30.09. – 01.11. 2006: Research visit in the Laboratory of Malaria and Vector Research, Vector Molecular Biology Unit ( the lab of Dr. Jesus G. Valenzuela, National Institute of Allergy and Infections Disease, Rockville, Maryland, U.S.A.); High-throughput approach to study of Ixodes ricinus salivary glands. Training in cDNA library construction, high-throughput approach in transcriptomics.
29.02. – 12.04. 2008: Research visit in the Laboratory of Malaria and Vector Research, Vector Biology Unit (the laboratory of Dr. J.M.C. Ribeiro, NIH/NIAID). Training in the preparation of recombinant serpins and their biochemical characterization
June 2008: ICTTD Bioinformatics Workshop, České Budějovice,
01.09.– 25.10. 2009: Research visit in Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry, the laboratory of Prof. Gunnar Pejler. Training in the isolation and cultivation of mouse peritoneum-derived and bone marrow-derived mast cells.

SELECTED CONFERENCES

21.09. – 26.09. 2008: 6th International Conference on Ticks and Tick-Borne Pathogens (Buenos Aires, Argentina), poster presentation „Functional and structural description of four new serpins from the tick Ixodes ricinus“
02.05-07.0502.05-07.05. 2010: Gordon Conference on Proteolytic Enzymes and Their Inhibitors (Il Ciocco, Barga, Italy). Poster presentation „IRS-2, a novel anti-inflammatory serpin from Ixodes ricinus

Publications
Total found: 12 records
Cabezas-Cruz A., Mateos-Hernández L., Chmelař J., Villar M., de la Fuente J. (2017) Salivary Prostaglandin E2: Role in Tick-Induced Allergy to Red Meat Trends in Parasitology 33 : 495-498.
DOI: 10.1016/j.pt.2017.03.004
Chmelař J., Kotál J., Langhansová H., Kotsyfakis M. (2017) Protease inhibitors in tick saliva: the role of serpins and cystatins in tick-host-pathogen interaction Frontiers in Cellular and Infection Microbiology 7 : 216.
DOI: 10.3389/fcimb.2017.00216
Caisová V., Vieru A., Kumžáková Z., Glaserová S., Husníková H., Vácová N., Krejčová G., Paďouková L., Jochmanová I., Wolf K.I., Chmelař J., Kopecký J., Ženka J. (2016) Innate immunity based cancer immunotherapy: B16-F10 murine melanoma model BMC Cancer 16 : 940.
DOI: 10.1186/s12885-016-2982-x
Chmelař J., Kotál J., Kopecký J., Pedra J., Kotsyfakis M. (2016) All for one and one for all on the tick–host battlefield Trends in Parasitology 32 : 368-377.
DOI: 10.1016/j.pt.2016.01.004
Kotál J., Langhansová H., Lieskovská J., Andersen J., Francischetti I., Chavakis T., Kopecký J., Pedra J., Kotsyfakis M., Chmelař J. (2015) Modulation of host immunity by tick saliva Journal of Proteomics 128 : 58-68.
DOI: 10.1016/j.jprot.2015.07.005
Páleníková J., Lieskovská J., Langhansová H., Kotsyfakis M., Chmelař J., Kopecký J. (2015) Ixodes ricinus Salivary Serpin IRS-2 Affects Th17 Differentiation via Inhibition of the Interleukin-6/STAT-3 Signaling Pathway Infection and Immunity 83 : 1949-1956.
DOI: 10.1128/IAI.03065-14
Chmelař J., Chung K., Chavakis T. (2013) The role of innate immune cells in obese adipose tissue inflammation and development of insulin resistance Thrombosis and Haemostasis 109 : 399-406.
DOI: 10.1160/TH12-09-0703
Chmelař J., Calvo E., Pedra J., Francischetti I., Kotsyfakis M. (2012) Tick salivary secretion as a source of antihemostatics. Journal of Proteomics 75 : 3842–3854.
DOI: 10.1016/j.jprot.2012.04.026
Chmelař J., Oliveira C., Řezáčová P., Francischetti I., Kovářová Z., Pejler G., Kopáček P., Ribeiro J., Mareš M., Kopecký J., Kotsyfakis M. (2011) A tick salivary protein targets cathepsin G and chymase and inhibits host inflammation and platelet aggregation. Blood 117 : 736–744.
DOI: 10.1182/blood-2010-06-293241
Kovářová Z., Chmelař J., Šanda M., Brynda J., Mareš M., Řezáčová P. (2010) Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus. Acta Crystallographica Section F 66 : 1453–1457.
DOI: 10.1107/S1744309110032343
Chmelař J., Kopecký J., Anderson J., Valenzuela J., Jochim R., Mu J. (2008) Insight into the sialome of the castor bean tick, Ixodes ricinus. BMC Genomics 20 : 233-0.
DOI: 10.1186/1471-2164-9-233
Salát J., Jelínek J., Chmelař J., Kopecký J. (2008) Efficacy of gamma interferon and specific antibody for treatment of microsporidiosis caused by Encephalitozoon cuniculi in SCID mice. Antimicrobial Agents and Chemotherapy 52 : 2169-2174.
DOI: 10.1128/AAC.01506-07

CONTACT

Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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