I. Workplace for applications of low voltage electron microscopy on biological specimens

II. New nanoparticles for ultrastructural diagnostics

The project aims to allow for the first time a sensitive simultaneous detection of at least four antigens.
It has three main aims:

1 Development of a set of nanoparticles sized 5-15 nm with various shapes or elemental composition,
suitable for ultrastructural cytochemistry. The result will be a set of at least four distinguishable electron-stable nanoparticles for simultaneous detection.
2 Preparation of a stable detection set of nanoparticles conjugated with antibodies for multiple ultrastructural labelings
in biomedicine, and a description of their properties for TEM and SEM. The detection set will be commercially used and distributed to the scientific community.
3 Development of a diagnostic tool and a standardized procedure for ultrastructural characterization of antigen spectra
in viral vaccines/fractions in mumps vaccine. The result will allow for a vaccine improvement and a control of presence of required immunization antigens; it will be commercially used.