RNDr. Eva Horáková, Ph.D.

Funkce: Vědecký pracovník - Laboratoř molekulární biologie prvoků

Kontaktní údaje

Telefon: +420 387775481
E-mail: horakova@paru.cas.cz
Místnost: PARU II, 1. patro

Odborný životopis

 

EDUCATION

2000-2005

Faculty of Biological Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic

Ph.D. In Molecular Biology

Thesis :“Functional analysis of mitochondrial proteins in Trypanosoma brucei procyclics.“

supervisor: Prof. Julius Lukes, email: jula@paru.cas.cz

1995-2000

Faculty of Science, Masaryk University, Brno, Czech Republic

M.S. In Molecular Biology and Genetics

Thesis: „Typization of HLA antigens in CVID patients“

supervisor: MUDr. Tomas Freiberger Ph.D., email: tomf@vuzd.cz

 

WORK EXPERIENCE

2010-present

Postdoctoral Fellow, Laboratory of Molecular Parasitology, Institute of Parasitology, Biology Centre, ASCR, v.v.i.

„Posttranscriptional modifications of mitochondrial and cytosolic tRNAs in Trypanosoma brucei

2009 – 2010

Postdoctoral Fellow,  McMaster laboratory, Department of Medical Genetics, Vancouver Coastal Health Research Institute,

University of British Columbia, Jack Bell Research Centre

“Proteomic Profiling of Host-Pathogen interaction: The Macrophage response to Leishmania major” Prof. Robert W. McMaster (robm@interchange.ubc.ca)

2006-2008 Maternity leave

2000-2005

Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic.

Research Scientist in Laboratory of Molecular Parasitology

2003 6 month research stay in Prof. Kenneth Stuart laboratory, Seattle, Biomedical Research Institute, Seattle, Washington, USA

2002 6 month research stay in Dr. Rob Benne laboratory, Department of Biochemistry, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands, supported by EURESCO Exchange Grant given to E.V.

1999-2000

Department of Imunogenetics, Research Institute of Child Health, Brno, Czech Republic bachelor

Student of Molecular Biology and Genetics

 

 

            Notable awards:

2007, Otto Wichterle Premium for young scientific team, AS CR

2005, honored PhD thesis “Functional analysis of mitochondrial proteins in Trypanosoma brucei procyclics”

2001, honored student grant “RNA interference in Trypanosoma brucei

 

ACADEMICAL COURSES

“Grant Writing for Funding and Research Development” University of British Columbia

             “Mass Spectrometry in Life Sciences,” University of British Columbia

“Proactive Communication & Effective Temwork Workshop” MITACS Skills

Training&Entrepreneurship Program (STEP)

 

SUPERVISION OF UNDERGRADUATE STUDENTS

2010 Melanie van Soeren: Characterization of the Leishmania protein GP63 using site direct mutagenesis

 

LABORATORY SKILLS

Wide range of general lab experience, focusing on molecular biology and proteomics:

Culture work (Leishmania ssp, Trypanosoma ssp.), Culture manipulation, Transfection studies, Macrophage infection studies, THP-1 cell cultivation, Peritoneal macrophage cell harvest.

Isolation of amastigotes from spleen/lesion, axenic cultivation of amastigotes

Leishmaniainfection rate. Limited Dilution assay. Spleen/Liver smears (H/E staining)

Viability assays (MTS assay, Alamar Blue assay)

Mass spectrometry sample preparation. Operating ABI 4800 mass spec, Protein Pilot, Statistical analysis of proteomics data.

Site-direct mutagenesis

Gelatin zymography

FACS analysis.

Isolation of DNA, RNA and mitochondrial vesicles

gel electrophoresis, SDS-PAGE,, 2D gel electrophoresis

Western and Northern blotting

Radiolabelling, Imunochemical staining

RNA interference of Trypanosoma brucei

immunofluorescent microscopy

PCR, RT-PCR, Quantitative RT-PCR, poisoned primer extension

in vitro editing

measurement of mitochondrial membrane potential

measurement of respiration rate

histochemical staining of protein complexes

NADH dehydrogenase, cytochrome creductase, and oxidase activity assays

 

Publikace
Celkem nalezeno: 13 záznamů
Haindrich A., Boudová M., Vancová M., Pena Diaz C., Horáková E., Lukeš J. (2017) The intermembrane space protein Erv1 of Trypanosoma brucei is essential for mitochondrial Fe-S cluster assembly and operates alone Molecular and Biochemical Parasitology 214 : 47-51.
DOI: 10.1016/j.molbiopara.2017.03.009

Dokumenty ke stažení:
Haindrich et al. 2017 MBP (pdf)
Horáková E., Changmai P., Vancová M., Sobotka R., Van Den Abbeele J., Vanhollebeke B., Lukeš J. (2017) The Trypanosoma brucei TbHrg protein is a heme transporter involved in regulation of stage-specific morphological transitions The Journal of Biological Chemistry 292 : 6998-7010.
DOI: 10.1074/jbc.M116.762997

Dokumenty ke stažení:
Horakova et al. 2017 (pdf)
Kraeva N., Horáková E., Kostygov A., Kořený L., Butenko A., Yurchenko V., Lukeš J. (2017) Catalase in Leishmaniinae: With me or against me? Infection, Genetics and Evolution 50 : 121-127.
DOI: 10.1016/j.meegid.2016.06.054

Dokumenty ke stažení:
Kraeva et al. 2017 JBCh (pdf)
Basu S., Horáková E., Lukeš J. (2016) Iron-associated biology of Trypanosoma brucei. Biochimica et Biophysica Acta 1860 : 368-370.
DOI: 10.1016/j.bbagen.2015.10.027

Dokumenty ke stažení:
Basu et al. 2016 B et B Acta (pdf)
Horáková E., Changmai P., Paris Z., Salmon D., Lukeš J. (2015) Simultaneous depletion of Atm and Mdl rebalances cytosolic Fe-S cluster assembly but not heme import into the mitochondrion of Trypanosoma brucei FEBS Journal 282 : 4157-4175.
DOI: 10.1111/febs.13411

Dokumenty ke stažení:
Horkova et al. 2015 FEBS Journal (pdf)
Sample P., Kořený L., Paris Z., Gaston K., Rubio M., Fleming I., Hinger S., Horáková E., Limbach P., Lukeš J., Alfonzo J. (2015) A common tRNA modification at an unusual location: the discovery of wyosine biosynthesis in mitochondria Nucleic Acid Research 43 : 4262-4273.
DOI: 10.1093/nar/gkv286

Dokumenty ke stažení:
Sample_et_al_NAR_2015 (pdf)
Verner Z., Basu S., Benz C., Dixit S., Dobáková E., Faktorová D., Hashimi H., Horáková E., Huang Z., Paris Z., Pena P., Ridlon L., Týč J., Wildridge D., Zíková A., Lukeš J. (2015) The malleable mitochondrion of Trypanosoma brucei Internation Review of Cell and Molecular Biology 315 : 73-151.
DOI: 10.1016/bs.ircmb.2014.11.001

Dokumenty ke stažení:
Verner-IRCMB-2015 (pdf)
Kovářová J., Horáková E., Changmai P., Vancová M., Lukeš J. (2014) Mitochondrial and nucleolar localization of cysteine desulfurase Nfs and the scaffold protein Isu in Trypanosoma brucei Eukaryotic Cell 13 : 353-362.
DOI: 10.1128/EC.00235-13

Dokumenty ke stažení:
Kovarova-EC-2014 (pdf)
Changmai P., Horáková E., Long S., Černotíková-Stříbrná E., Mcdonald L., Bontempi E., Lukeš J. (2013) Both human ferredoxins equally efficiently rescue ferredoxin deficiency in Trypanosoma brucei Molecular microbiology 89 : 135-151.
DOI: 10.1111/mmi.12264

Dokumenty ke stažení:
2013_Changmai_MM_135 (pdf)
Paris Z., Horáková E., Rubio M., Sample P., Fleming I., Armocida S., Lukeš J., Alfonzo J. (2013) The T. brucei TRM5 methyltransferase plays an essential role in mitochondrial protein synthesis and function. RNA 19 : 649-658.


Dokumenty ke stažení:
2013_Paris_RNA_649 (pdf)
Šmíd O., Horáková E., Vilímová V., Hrdý I., Cammack R., Horváth A., Lukeš J., Tachezy J. (2006) Knock-downs of mitochondrial iron-sulfur cluster assembly proteins IscS and IscU down-regulate the active mitochondrion of procyclic Trypanosoma brucei. Journal of Biological Chemistry 281 : 28679-28686.
DOI: 10.1074/jbc.M513781200

Dokumenty ke stažení:
Smid et al. 2006 JBCh (pdf)
Zíková A., Horáková E., Jirků M., Dunajčíková P., Lukeš J. (2006) The effect of down-regulation of mitochondrial RNA-binding proteins MRP1 and MRP2 on respiratory complexes in procyclic Trypanosoma brucei. Molecular and Biochemical Parasitology 149 : 65-73.
DOI: 10.1016/j.molbiopara.2006.04.007

Dokumenty ke stažení:
Zikova et al. 2006 MBP (pdf)
Horváth A., Horáková E., Dunajčíková P., Verner Z., Pravdová E., Šlapetová I., Cuninková Ľ., Lukeš J. (2005) Down-regulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei. Molecular Microbiology 58 : 116-130.
DOI: 10.1111/j.1365-2958.2005.04813.x

Dokumenty ke stažení:
Horvath et al. 2005 MM (pdf)

KONTAKT

Biologické centrum AV ČR, v.v.i.
Parazitologický ústav
Branišovská 1160/31
370 05 České Budějovice

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