RNDr. Zdeněk Paris, Ph.D.

Funkce: Vedoucí laboratoře - Laboratoř RNA biologie prvoků

Kontaktní údaje

Telefon: +420 387775427
E-mail: parda@paru.cas.cz
Místnost: PARU II, 1. patro

Popis

Iron-sulfur clusters (ISC) are part of the active site of many enzymes and are essential for cell viability. Our study is focused on de novo synthesis of ISC in mitochondrion of Trypanosoma brucei. Additionally, thanks to wonderful collaboration with Juan D Alfonzo (Ohio State University, Columbus, Ohio, USA) we are also interested in the role of the ISC pathway on tRNA modifications and editing.

Odborný životopis

VZDĚLÁNÍ
Přírodovědecká fakulta Jihočeské univerzity v Českých Budějovicích

  • Magisterská práce: Expresní systém u Leishmania tarentolae. Obhájeno: květen 2006
  • Doktorandská práce: Identifikace nových genů, kódujících proteiny dýchacího řetězce a proteiny podílející se na tvorbě Fe-S center u Trypanosoma brucei.  Obhájeno: září 2010.

Publikace
Celkem nalezeno: 18 záznamů
Kessler A.C., Kulkarni S., Paulines M.J., Rubio M., Limbach P., Paris Z., Alfonzo J. (2017) Retrograde nuclear transport from the cytoplasm is required for tRNATyr maturation in T. brucei RNA Biology DOI: 10.1080/15476286.2017.1377878
Rubio M., Gaston K., McKenney K., Fleming I., Paris Z., Limbach P., Alfonzo J. (2017) Editing and methylation at a single site by functionally interdependent activities Nature 542 : 494-497.
DOI: 10.1038/nature21396
Fleming I., Paris Z., Gaston K., Gaston K., Balakrishnan R., Fredrick K., Rubio M., Alfonzo J. (2016) A tRNA methyltransferase paralog is important for ribosome stability and cell division in Trypanosoma brucei Scientific Reports 6 : 21438.
DOI: 10.1038/srep21438
Lopes R., Silveira G., Eitler R., Vidal R., Kessler A., Hinger S., Paris Z., Alfonzo J., Polycarpo C. (2016) The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr. RNA 22 : 1190-1199.
DOI: 10.1261/rna.056242.116
Zíková A., Hampl V., Paris Z., Týč J., Lukeš J. (2016) Aerobic mitochondria of parasitic protists: diverse genomes and complex functions. Molecular and Biochemical Parasitology 209 : 46-57.
DOI: 10.1016/j.molbiopara.2016.02.007

Dokumenty ke stažení:
Zikova et al. 2016 MBP (pdf)
Horáková E., Changmai P., Paris Z., Salmon D., Lukeš J. (2015) Simultaneous depletion of Atm and Mdl rebalances cytosolic Fe-S cluster assembly but not heme import into the mitochondrion of Trypanosoma brucei FEBS Journal 282 : 4157-4175.
DOI: 10.1111/febs.13411

Dokumenty ke stažení:
Horkova et al. 2015 FEBS Journal (pdf)
Sample P., Kořený L., Paris Z., Gaston K., Rubio M., Fleming I., Hinger S., Horáková E., Limbach P., Lukeš J., Alfonzo J. (2015) A common tRNA modification at an unusual location: the discovery of wyosine biosynthesis in mitochondria Nucleic Acid Research 43 : 4262-4273.
DOI: 10.1093/nar/gkv286

Dokumenty ke stažení:
Sample_et_al_NAR_2015 (pdf)
Verner Z., Basu S., Benz C., Dixit S., Dobáková E., Faktorová D., Hashimi H., Horáková E., Huang Z., Paris Z., Pena P., Ridlon L., Týč J., Wildridge D., Zíková A., Lukeš J. (2015) The malleable mitochondrion of Trypanosoma brucei Internation Review of Cell and Molecular Biology 315 : 73-151.
DOI: 10.1016/bs.ircmb.2014.11.001

Dokumenty ke stažení:
Verner-IRCMB-2015 (pdf)
Paris Z., Horáková E., Rubio M., Sample P., Fleming I., Armocida S., Lukeš J., Alfonzo J. (2013) The T. brucei TRM5 methyltransferase plays an essential role in mitochondrial protein synthesis and function. RNA 19 : 649-658.


Dokumenty ke stažení:
2013_Paris_RNA_649 (pdf)
Rubio M., Paris Z., Gaston K., Fleming I., Sample P., Trotta C., Alfonzo J. (2013) Unusual Noncanonical Intron Editing Is Important for tRNA Splicing in Trypanosoma brucei Molecular cell 52 : 184-192.
DOI: 10.1016/j.molcel.2013.08.042
Gnipová A., Panicucci B., Paris Z., Verner Z., Horváth A., Lukeš J., Zíková A. (2012) Disparate phenotypic effects from the knockdown of various Trypanosoma brucei cytochrome c oxidase subunits. Molecular and Biochemical Parasitology 184 : 90-98.
DOI: 10.1016/j.molbiopara.2012.04.013

Dokumenty ke stažení:
2012_Gnipova_MBP_90 (pdf)
Paris Z., Hashimi H., Lun S., Alfonzo J., Lukeš J. (2011) Futile import of tRNAs and proteins into the mitochondrion of Trypanosoma brucei evansi. Molecular and Biochemical Parasitology 176 : 116–120.
DOI: 10.1016/j.molbiopara.2010.12.010

Dokumenty ke stažení:
Paris et al. 2011 MBP (pdf)
Paris Z., Changmai P., Rubio M., Zíková A., Stuart K., Alfonzo J., Lukeš J. (2010) The Fe/S Cluster assembly protein Isd11 is essential for tRNA thiolation in Trypanosoma brucei. Journal of Biological Chemistry 285 : 22394–22402.
DOI: 10.1074/jbc.M109.083774

Dokumenty ke stažení:
Paris et al. 2010 JBCh (pdf)
Verner Z., Paris Z., Lukeš J. (2010) Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei. Parasitology Research 106 : 1241–1244.
DOI: 10.1007/s00436-010-1754-9

Dokumenty ke stažení:
Verner et al. 2010 PR (pdf)
Paris Z., Rubio M., Lukeš J., Alfonzo J. (2009) Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein. RNA 15 : 1398-1406.
DOI: 10.1261/rna.1589109

Dokumenty ke stažení:
Paris et al. 2009 RNA (pdf)
Wohlgamuth-Benedum J., Rubio M., Paris Z., Long S., Poliak P., Lukeš J., Alfonzo J. (2009) Thiolation controls cytoplasmic tRNA stability and acts as a negative determinant for tRNA editing in mitochondria. Journal of Biological Chemistry 284 : 23974-23953.
DOI: 10.1074/jbc.M109.029421

Dokumenty ke stažení:
Wolgamuth-Benedum et al. 2009 JBCh (pdf)
Lukeš J., Paris Z., Regmi S., Breitling R., Mureev S., Kushnir S., Pyatkov K., Jirků M., Alexandrov K. (2006) Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5‘ sequence context. Molecular and Biochemical Parasitology 148 : 125-132.
DOI: 10.1016/j.molbiopara.2006.03.008

Dokumenty ke stažení:
Lukes et al. 2006 MBP (pdf)
Foldynová-Trantírková S., Paris Z., Sturm N., Campbell D., Lukeš J. (2005) The Trypanosoma brucei La protein is a candidate poly(U) shield protein that impacts spliced leader RNA maturation and tRNA intron removal. International Journal for Parasitology 35 : 359-366.
DOI: 10.1016/j.ijpara.2004.12.012

Dokumenty ke stažení:
Foldynova-Trantirkova et al. 2005 IJP (pdf)

KONTAKT

Biologické centrum AV ČR, v.v.i.
Parazitologický ústav
Branišovská 1160/31
370 05 České Budějovice

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