RNDr. Aleš Tomčala, Ph.D.

Job Position: Research Scientist - Laboratory of Evolutionary Protistology

Contact details

Phone: +420 387775486
E-mail: tomcala@paru.cas.cz
Room: 238

Description

Research interests

  • analysis of lipidic compounds by HPLC and UPLC ESI MS/MS and GC MS and GC FID in biological samples - tissues and body fluids
  • membrane dynamics and its component analysis 
  • study ESI MS fragmentation of lipidic compounds of membrane
  • metabolomics and biochemical pathway tracking
Curriculum vitae

Education :

  • 2009 Faculty of Science, The University of South Bohemia in České Budějovice. PhD Thesis: Determination of membrane lipidic components in several biological matrixes by HPLC ESI MS/MS method
  • 2008 9. school of HPLC/MS take place in Seč set up by University of Pardubice and by Spectroscopic society
  • 2007 8. school of HPLC/MS take place in Lednice set up by University of Pardubice and by Spectroscopic society
  • 2007 Faculty of Science, The University of South Bohemia in České Budějovice. 
  • RNDr. Thesis: Seasonal acquisition of chill tolerance and restructuring of membrane
  • glycerophospholipids in an overwintering insect: triggering by low temperature, desiccation and diapause progression
  • 2005 6. school of HPLC/MS take place in Doubice set up by University of Pardubice and by Spectroscopic society. 
  • 2003-2005 Biological faculty, The University of South Bohemia in České Budějovice. Msc Thesis: Quantification and characterization of phospholipids in cell membrane of Pyrrhocoris apters (Heteroptera) and its remodulation during temperature changes and desiccation. 
  • 1999-2003 Biological faculty, The University of South Bohemia in České Budějovice. Bc. Thesis: Melatonine Function in Living Systems and Its Determination in Animals. 

Research projects:

  • Studium biologicky aktivních metabolitů technikou UPLC/MS - příprava nových metodik pro stanovení titru ekdysteroidů a juvenilních hormonů a pro separaci a identifikaci jednotlivých lipidických složek v komplexních biologických matricích. (Grant Agency of the University of South Bohemia; 2007).

Employment history:

  • 2012 –  Postdoct position in Laboratory of Evolution Protistology, Institute of Parasitology, Biological center, v.i.i., Academy of Sciences of Czech Republic, České Budějovice.    
  • 2010 – 2012 - Postdoct position in Laboratory of natural products, Institute of Organic Chemistry and Biochemistry, v.v.i., Academy of Sciences of Czech Republic, Prague.
  • 2005 – 2009 - Laboratory of Analytical Biochemistry, Institute of Entomology, Biological center, v.i.i., Academy of Sciences of Czech Republic, České Budějovice.

Recent publication: 

  • Goncalves GB, Silva CE, Mendonca AD, Vanickova L, Tomcala A, Do Nascimento RR. PHEROMONE COMMUNICATION IN ANASTREPHA OBLIQUA (DIPTERA: TEPHRITIDAE): A COMPARISON OF THE VOLATILES AND SALIVARY GLAND EXTRACTS OF TWO WILD POPULATIONS. Fla Entomol. 2013;96(4):1365-74.
  • Brirzova R, Mendonca AL, Vanickova L, Da Silva CE, Tomcala A, Paranhos BAJ, et al. PHEROMONE ANALYSES OF THE ANASTREPHA FRATERCULUS (DIPTERA: TEPHRITIDAE) CRYPTIC SPECIES COMPLEX. Fla Entomol. 2013;96(3):1107-15.
  • Vanickova L, do Nascimento RR, Hoskovec M, Jezkova Z, Brizova R, Tomcala A, et al. Are the Wild and Laboratory Insect Populations Different in Semiochemical Emission? The Case of the Medfly Sex Pheromone. J Agric Food Chem. 2012;60(29):7168-76.
  • Kodrik D, Vinokurov K, Tomcala A, Socha R. The effect of adipokinetic hormone on midgut characteristics in Pyrrhocoris apterus L. (Heteroptera). J Insect Physiol. 2012;58(1):194-204.
  • Cvacka J, Jiros P, Kalinova B, Straka J, Cerna K, Sebesta P, Tomcala A,Vasickova S, Jahn U, Sobotnik J. Stylopsal: The First Identified Female-produced Sex Pheromone of Strepsiptera. J Chem Ecol. 2012;38(12):1483-91.
  • Kostal V, Korbelova J, Rozsypal J, Zahradnickova H, Cimlova J, Tomcala A, et al. Long-Term Cold Acclimation Extends Survival Time at 0 degrees C and Modifies the Metabolomic Profiles of the Larvae of the Fruit Fly Drosophila melanogaster. PLoS One. 2011;6(9).
  • A.Tomcala, I.Bartu, P.Simek, D.Kodrik: Locust adipokinetic hormones mobilize diacylglycerols selectively. Comparative Biochemistry and Physiology, Part B 156, 26-32, 2010.  
  • Bártů I., Tomčala A., Socha R., Šimek P., Kodrík D.: Analysis of lipids mobilized by adipokinetic hormones in the firebug Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae) Eur. J. Entomol. 107: 509-520, 2010. 

Publications
Total found: 31 records
Morgantini C., Jager J., Li X., Levi L., Azzimato V., Sulen A., Barreby E., Xu C., Tencerová M., Näslund E., Kumar Ch., Verdeguer F., Straniero S., Hultenby K., Björkström N., Ellis E., Rydén M., Kutter C., Hurrell T., Lauschke V.M., Boucher J., Tomčala A., Krejčová G., Bajgar A., Aouadi M. (2019) Liver macrophages regulate systemic metabolism through non-inflammatory factors Nature Metabolism 1 : 445–459.
DOI: 10.1038/s42255-019-0044-9
Bednářová A., Tomčala A., Mochanová M., Kodrík D., Krishnan N. (2018) Disruption of adipokinetic hormone mediated energy homeostasis has subtle effects on physiology, behaviour and lipid status during aging in Drosophila. Frontiers in Physiology 9 : article no. 949.
DOI: 10.3389/fphys.2018.00949
Mochanová M., Tomčala A., Svobodová Z., Kodrík D. (2018) Role of adipokinetic hormone during starvation in Drosophila. Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology 226 : 26–35.
DOI: 10.1016/j.cbpb.2018.08.004
Tomčala A., Jirošová A., Žáček P., Kaušková M., Hovorka O., Koutek B. (2017) Species specificity of aldehyde and fatty acid profiles of four family group representatives within the insect infraorder Pentatomomorpha (Hemiptera: Heteroptera) Chemistry & Biodiversity 14 : e1600420.
DOI: 10.1002/cbdv.201600420
Tomčala A., Kyselová V., Schneedorferová I., Opekarová I., Moos M., Urajová P., Kručinská J., Oborník M. (2017) Separation and identification of lipids in the photosynthetic cousins of Apicomplexa Chromera velia and Vitrella brassicaformis. Journal of Separation Science 40 : 3402-3413.
DOI: 10.1002/jssc.201700171
Bajgar A., Kučerová K., Jonátová L., Tomčala A., Schneedorferová I., Okrouhlík J., Doležal T. (2015) Extracellular Adenosine Mediates a Systemic Metabolic Switch during Immune Response PLOS Biology 13 : e1002135.
DOI: 10.1371/journal.pbio.1002135
Flegontov P., Janouškovec J., Lai H., Jirků M., Hajdušková E., Tomčala A., Otto T.D., Keeling P., Pain A., Oborník M., Lukeš J. (2015) Divergent Mitochondrial Respiratory Chains in Phototrophic Relatives of Apicomplexan Parasites. Molecular Biology and Evolution 32 : 1115-1131.
DOI: 10.1093/molbev/msv021

Documents to download:
Flegontov et al. 2015 MBE (pdf)
Kopečná J., Pilný J., Krynická V., Tomčala A., Kis M., Gombos Z., Komenda J., Sobotka R. (2015) Lack of Phosphatidylglycerol Inhibits Chlorophyll Biosynthesis at Multiple Sites and Limits Chlorophyllide Reutilization in Synechocystis sp. Strain PCC 6803 Plant Physiology 169 : 1307-1317.
DOI: 10.1104/pp.15.01150
Schneedorferová I., Tomčala A., Valterová I. (2015) Effect of heat treatment on the n-3/n-6 ratio and content of polyunsaturated fatty acids in fish tissues Food Chemistry 176 : 205-211.
DOI: 10.1016/j.foodchem.2014.12.058
Votavová A., Tomčala A., Kofroňová E., Kudzejová M., Šobotník J., Jiroš P., Komzáková O., Valterová I. (2015) Seasonal Dynamics in the Chemistry and Structure of the Fat Bodies of Bumblebee Queens PLoS ONE 10 : e0142261.
DOI: 10.1371/journal.pone.0142261
Weyda F., Pflegerová J., Stašková T., Tomčala A., Prenerová E., Zemek R., Volter L., Kodrík D. (2015) Ultrastructural and biochemical comparison of summer active and summer diapausing pupae of the horse chestnut leaf miner Cameraria ohridella (Lepidoptera: Gracillariidae). Eur. J. Entomol. 112 : 197-203.
DOI: 10.14411/eje.2015.014
Woo Y., Ansari H., Otto T.D., Klinger C., Kolísko M., Saxena A., Shanmugam D., Tayyrov A., Veluchamy A., Ali S., Bernal A., Del C., Cihlář J., Flegontov P., Gornik S., Hajdušková E., Horák A., Janouškovec J., Katris N., Mast F., Miranda-Saavedra D., Mourier T., Naeem R., Nair M., Panigrahi A., Rawlings N., Regelado E., Ramaprasad A., Samad N., Tomčala A., Wilkes J., Neafsey D., Doerig C., Bowler C., Keeling P., Roos D., Dacks J., Templeton T., Waller R., Lukeš J., Oborník M., Pain A. (2015) "Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites " eLife 4 : e06974.
DOI: 10.7554/eLife.06974

Documents to download:
Woo_et_al_eLife_2015 (pdf)
Vinokurov K., Bednářová A., Tomčala A., Stašková T., Krishnan N., Kodrík D. (2014) Role of adipokinetic hormone in stimulation of salivary gland activities: the fire bug Pyrrhocoris apterus L. (Heteroptera) as a model species. J. Insect Physiol. 60 : 58-67.
DOI: 10.1016/j.jinsphys.2013.11.005
Zahradníčková H., Tomčala A., Berková P., Schneedorferová I., Okrouhlík J., Šimek P., Hodková M. (2014) Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: Application to insects. Journal of Separation Science 37 : 2062-2068.
DOI: 10.1002/jssc.201400113
Kodrík D., Tomčala A., Bártů I., Socha R. (2012) Insect lipids mobilized by adipokinetic hormones. In: Langella J.P. (Ed.), Saturated Fats: Metabolism, Disease Risks and Public Awareness, Nova Science Publishers, Inc., New York, ISBN: 978-1-61209-566-0 p. 99-121.
Kodrík D., Vinokurov K., Tomčala A., Socha R. (2012) The effect of adipokinetic hormone on midgut characteristics in Pyrrhocoris apterus L. (Heteroptera) J. Insect. Physiol. 58 : 194-204.
DOI: 10.1016/j.jinsphys.2011.11.010
Kodrík D., Vinokurov K., Tomčala A., Socha R. (2012) Digestive enzymes and AKH control of their activities in Pyrrhocoris apterus (Heteroptera). 26th Conference of European Comparative Endocrinologists, Zurich, Switzerland, August 21 - 25, Abstract No. OR 14-6 p. 79.
Kodrík D., Vinokurov K., Tomčala A., Socha R. (2011) Control of insect digestion: Are adipokinetic hormones involved? In: VIIth International Conference on Arthropods: Chemical, Physiological, Biotechnological and Environmental Aspects (Bialka Tatrzanska, Poland, September 18-23) Abstract Book, Eds. D. Konopinska and M. Kuczer, ISBN: 978-83-60043-16-5, O-34 p. 46.
Košťál V., Korbelová J., Rozsypal J., Zahradníčková H., Cimlová J., Tomčala A., Šimek P. (2011) Long-term cold acclimation extends survival time at 0°C and modifies the metabolomic profiles of the larvae of the fruit fly Drosophila melanogaster. PLoS ONE 6 : e25025.
DOI: 10.1371/journal.pone.0025025
Tomčala A., Kindl J., Schneedorferová I., Kodrík D., Valterová I. (2011) LC/MS and GC FID techniques employed in the analyses of lipids involved in insect metabolic and adaptation processes In: VIIth International Conference on Arthropods: Chemical, Physiological, Biotechnological and Environmental Aspects (Bialka Tatrzanska, Poland, September 18-23) Abstract Book, Eds. D. Konopinska and M. Kuczer, ISBN: 978-83-60043-16-5, P-5 p. 65.

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CONTACT

Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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