RNDr. Ján Štěrba, Ph.D.
Job Position: Postdoc - Laboratory of Molecular Ecology of Vectors and Pathogens
Phone:
+420 387776220
E-mail:
sterbaj@paru.cas.cz
Room:
PrF C 01 008
Postdoctoral research fellow
Institute of Parasitology
Biology Centre of the Academy of Sciences of the Czech Republic
&
Assistant professor, Deputy head
Institute of Chemistry and Biochemistry
Faculty of Science, University of South Bohemia
Research interests:
Glycobiology of ticks and tick-borne pathogens, allergenogenic arthropod glycans, mass spectrometry, bioorthogonal chemistry
Education and employment:
2002: BSc. in Chemistry, Faculty of Sciences, University of P. J. Šafárik, Košice, Slovak Republic
2003: studies at Laboratory of Biochemistry, Faculty of Chemistry, Aristoteles University in Thessaloniki within Socrates-Erasmus exchange program
2004: graduated with honors from chemistry (specialization biochemistry), Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, University of P. J. Šafárik, Košice, Slovak Republic; award of the dean of Faculty of Natural Sciences, University of P. J. Šafárik.
2004: Laboratory of Clinical Biochemistry, Health Centre Veľké Kapušany, Veľké Kapušany, Slovak Republic
2004-2012: Ph.D. studies in Molecular and Cellular Biology and Genetics at the Faculty of Biological Sciences (now Faculty of Science), University of South Bohemia, České Budějovice, Czech Republic
2004-2006: research assistant, Biology Centre of Academy of Sciences of the Czech Republic v.v.i., Institute of Parasitology, České Budějovice, Czech Republic
2006: assistant at Faculty of Biological Sciences, USB, České Budějovice
2006: visiting scientist, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada
2007-: assistant professor at Faculty of Biological Sciences, USB, České Budějovice; now Faculty of Science, USB, České Budějovice
2007: visiting scientist, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada
2009, 2010: visiting scientist, National Center for Glycomics and Glycoproteomics, Department of Chemistry, Indiana University, Bloomington, Indiana, USA
2010-: postdoctoral research fellow, Institute of Parasitology, Biology Centre of ASCR, České Budějovice
2014/2015: research stay, Genomics group, SaBio (Sanidad y Biotecnología) research group, National Wildlife Research Institute IREC (CSIC-Universidad de Castilla – La Mancha), Ciudad Real, Spain.
Teaching(at Faculty of Science, University of South Bohemia):
Pharmacology and Toxicology (lectures + practical course, in Czech) and Introduction to Toxicology (lectures + practical course, in Czech);
Xenobiochemistry and Toxicology (lectures + practical course, in English);
Laboratory Course in Biochemistry (in Czech and in English), and Adnavced Course in Bicohemistry (in Czech and in English);
supervisor and co-supervisor of 20 bachelor and masters students, co-supervisor of a student from University of Glasgow (work placement at Faculty of Science, University of South Bohemia)
Published scientific papers:
Růžek D., Kopecký J., Štěrba J., Golovchenko M., Rudenko N., Grubhoffer L.: Non-virulent strains of TBE virus circulating in the Czech Republic. Journal of Clinical Virology 36 (suppl. 2): S41, 2006 (P117).
Růžek D., Štěrba J., Kopecký J., Grubhoffer L.: The supposedly attenuated Hy-HK variant of highly virulent Hypr strain of tick-borne encephalitis virus is obviously a stain of Langat virus. Acta Virologica 50(4), 227-278, 2006.
Štěrba J., Vancová M., Rudenko N., Golovchenko M., Tremblay T.-L., Kelly J.F., MacKenzie R.C., Logan S.M., Grubhoffer L.: Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated. Journal of Bacteriology 190: 2619-2623, 2008.
Man P., Kovář V., Štěrba J., Strohalm M., Kavan D., Kopáček P., Grubhoffer L., Havlíček V.: Deciphering Dorin M glycosylation by mass spectrometry. European Journal of Mass Spectrometry 14: 345-354, 2008.
Štěrba J., Dupejová J., Fišer M., Golovchenko M., Rudenko N., Grubhoffer L.: Identification and characterisation of lectins in several tick species. FEBS Journal, 276 (Suppl. 1): 88, 2009.
Grubhoffer L., Hajdušek O., Vancová M., Štěrba J., Rudenko N.: Glycobiochemistry of ticks, vectors of infectious diseases: carbohydrate-binding proteins and glycans. FEBS Journal, 276 (Suppl. 1): 141, 2009.
Dupejova J., Sterba J., Vancova M., Grubhoffer L.: Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization. Parasites&Vectors, 4:4, 2011.
Sterba J., Dupejova J., Fiser M., Vancova M., Grubhoffer L.: Fibrinogen-related proteins in ixodid ticks. Parasites&Vectors, 4:127, 2011.
Vancova M., Sterba J., Dupejova J., Simonova Z., Nebesarova J., Novotny M.V., Grubhoffer L.: Uptake and incorporation of sialic acid by the tick Ixodes ricinus. Journal of Insect Physiology, 2012, 58, 1277-1287.
Grubhoffer L., Rudenko N., Vancova M., Golovchenko M., Sterba J.: Circulatory system and hemolymph. In: Biology of ticks. Vol. 1. Daniel E. Sonenshine (Editor), R. Michael Roe (Editor). Oxford University Press Inc; 2013, 2nd Revised edition. pp. 258-286.
Sterba J., Vancova M., Sterbova J., Bell-Sakyi L., Grubhoffer L.: The majority of sialylated glycoproteins in adult Ixodes ricinus ticks originate in the host, not the tick. Carbohydr Res, 2014, 389, 93-99.
Hönig V, Svec P, Halas P, Vavruskova Z, Tykalova H, Kilian P, Vetiskova V, Dornakova V, Sterbova J, Simonova Z, Erhart J, Sterba J, Golovchenko M, Rudenko N, Grubhoffer L.: Ticks and tick-borne pathogens in South Bohemia (Czech Republic) - Spatial variability in Ixodes ricinus abundance, Borrelia burgdorferi and tick-borne encephalitis virus prevalence. Ticks Tick Borne Dis, 2015, in press.
Attended conferences:
more than 25 poster presentations and 6 lectures on international conferences
Projects:
2006: Glycosylation of tick-borne encephalitis virus envelope protein and its influence on virus virulence – Grantová agentura JU (PI),
2007: Glycosylation of membrane proteins of Lyme borreliosis spirochetes – Grantová agentura JU (PI),
2009-2011: Glycoprotein analysis of Ixodes ricinus salivary glands – GA AVČR (PI: Dr. Marie Vancová, Institute of Parasitology, BC ASCR, České Budějovice, co-PI: Ján Štěrba, Faculty of Science, University of South Bohemia, České Budějovice),
2016-2022: Research infrastructure C4SYS – MŠMT (PI: R. Ettrich, J. Štěrba – head of one of the research units, member of the Steering Committee).
Team member:
2008-2010: Molecular pathogenesis of tick-borne encephalitis – GAČR,
2009-2011: Interaction of Lyme borreliosis spirochetes and salivary glands of Ixodes ricinus tick: the role of borrelia OspC protein – GAČR,
2005-2011: Centre of molecular ecology of vectors and pathogens – MŠMT,
2010: Distribution and diversity of B. bissettii in the southeastern USA and in Europe – U. S. Civilian Research & Development Foundation,
2015-2017: Novel functions of viral and cellular proteins in tick-borne encephalitis virus infection – GAČR.
| Total found: 31 records
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Kotsarenko K.,
Věchtová P., Hammerová Z., Langová N., Malinovská L., Wimmerová M., Štěrba J.,
Grubhoffer L. (2020) Newly identified DNA methyltransferases of Ixodes ricinus ticks Ticks and Tick-borne Diseases
11
: 101348. DOI: 10.1016/j.ttbdis.2019.101348 |
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Kotsarenko K.,
Věchtová P., Lieskovská J., Fussy Z., Cabral-de-Mello D.C.,
Rego R. O. M., Alberdi P., Collins M., Bell-Sakyi L., Štěrba J.,
Grubhoffer L. (2020) Karyotype changes in long-term cultured tick cell lines Scientific Reports
10
: 13443. DOI: 10.1038/s41598-020-70330-5 |
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Loginov D., Böttinger K., Loginova Y.F., Dycka F.,
Věchtová P., Štěrba J. (2020) Biotyping of IRE/CTVM19 tick cell line infected by tick-borne encephalitis virus. Ticks and Tick-borne Diseases
11
: 101420. DOI: 10.1016/j.ttbdis.2020.101420 |
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Prysiazhnyi V., Dycka F., Kratochvíl J., Štěrba J., Straňák V. (2020) Gas-aggregated Ag nanoparticles for detection of small molecules using LDI MS. Analytical and Bioanalytical Chemistry
412
: 1037-1047. DOI: 10.1007/s00216-019-02329-5 |
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Rusanov A.L., Stepanov A.A., Zgoda V.G., Kaysheva A.L.,
Selinger M.,
Mašková H.,
Loginov D., Štěrba J.,
Grubhoffer L., Luzgina N.G. (2020) Proteome dataset of mouse macrophage cell line infected with tick-borne encephalitis virus. Data in Brief
28
: 105029. DOI: 10.1016/j.dib.2019.105029 |
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Vaidulych M., Pleskunov P., Kratochvíl J.,
Mašková H.,
Kočová_LMEVP P., Nikitin D., Hanuš J., Kylián O., Štěrba J., Biederman H. (2020) Convex vs concave surface nano-curvature of Ta2O5thin films for tailoringthe osteoblast adhesion Surface & Coatings Technology
393
: 125805. DOI: 10.1016/j.surfcoat.2020.125805 |
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Věchtová P., Fussy Z., Štěrba J.,
Erhart J., Beneš V.,
Grubhoffer L. (2020) Catalogue of stage-specifc transcripts in Ixodes ricinus and their potential functions during the tick life-cycle Parasites & Vectors
13
: 311. DOI: 10.1186/s13071-020-04173-4 |
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Dzyuba V., Sampels S., Ninhaus-Silveira A., Kahanec M., Veríssimo-Silveira R., Rodina M., Cosson J., Boryshpolets S.,
Selinger M., Štěrba J., Dzyuba B. (2019) Sperm motility and lipid composition in internally fertilizing ocellate river stingray Potamotrygon motoro. Theriogenology
130
: 26–35. DOI: 10.1016/j.theriogenology.2019.02.029 |
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Kratochvíl J., Kahoun D., Kylián O., Štěrba J., Kretková T., Kousal J., Hanuš J., Vaclová J., Prysiazhnyi V., Sezemský P., Fojtíková P.,
Lieskovská J.,
Langhansová H., Krakovský I., Straňák V. (2019) Nitrogen enriched C:H:N:O thin films for improved antibiotics doping Applied Surface Science
494
: 301–308. DOI: 10.1016/j.apsusc.2019.07.135 |
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Loginov D.S., Loginova Y.F., Dycka F., Böttinger K.,
Věchtová P., Štěrba J. (2019) Tissue-specific signatures in tick cell line MS profiles Parasites & Vectors
12
: 212. DOI: 10.1186/s13071-019-3460-5 |
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Selinger M.,
Tykalová H., Štěrba J.,
Věchtová P.,
Vavrušková Z.,
Lieskovská J., Kohl A., Schnettler E.,
Grubhoffer L. (2019) Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. Plos Neglected Tropical Diseases
27
: e0007745. DOI: 10.1371/journal.pntd.0007745 |
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Vaidulych M., Shelemin A., Hanuš J., Khalakhan I., Krakovský I., Kočová P., Mašková H., Kratochvíl J., Pleskunov P., Štěrba J., Kylián O., Choukourov A., Biderman H.,
Kočová_LMEVP P. (2019) Superwettable antibacterial textiles for versatile oil/water separation Plasma Processes and Polymers
16
: e1900003. DOI: 10.1002/ppap.201900003 |
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Xin M.,
Věchtová P., Shaliutina-Kolešová A., Fussy Z.,
Loginov D., Dzyuba B., Linhart O., Boryshpolets S., Rodina M., Li P., Loginova Y., Štěrba J. (2019) Transferrin Identification in Sterlet (Acipenser ruthenus) Reproductive System Animals
9
: 753. DOI: 10.3390/ani9100753 |
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Kratochvíl J., Kahoun D., Štěrba J.,
Langhansová H.,
Lieskovská J., Fojtíková P., Hanuš J., Kousal J., Kylián O., Straňák V. (2018) Plasma polymerized C:H:N:O thin films for controlled release of antibiotic substances Plasma Processes and Polymers
15
: 1700160. DOI: 10.1002/ppap.201700160 |
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Kratochvíl J., Štěrba J.,
Lieskovská J.,
Langhansová H., Kuzminova A., Khalakhan I., Kylián O., Straňák V. (2018) Antibacterial effect of Cu/C:F nanocomposites deposited on PEEK substrates Materials Letters
230
: 96–99. DOI: 10.1016/j.matlet.2018.07.082 |
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Věchtová P.,
Štěrbová J., Štěrba J.,
Vancová M.,
Rego R. O. M.,
Selinger M.,
Strnad M.,
Golovchenko M.,
Rudenko N.,
Grubhoffer L. (2018) A bite so sweet: the glycobiology interface of tick-host-pathogen interactions Parasites & Vectors
11
: 594. DOI: 10.1186/s13071-018-3062-7 |
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Xin M., Shaliutina-Kolešová A., Štěrba J.,
Koník P., Boryshpolets S., Rodina M., Li P., Nian R., Linhart O. (2018) Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome. Animal Reproduction Science
192
: 280–289. DOI: 10.1016/j.anireprosci.2018.03.025 |
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Xin M., Štěrba J., Shaliutina-Kolešová A., Dzyuba B.,
Lieskovská J., Boryshpolets S., Siddique M.A.M., Kholodnyy V., Lebeda I., Linhart O. (2018) Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation Fish Physiology and Biochemistry
44
: 1527-1533. DOI: 10.1007/s10695-018-0538-5 |
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Černý J.,
Selinger M.,
Palus M.,
Vavrušková Z.,
Tykalová H., Bell-Sakyi L., Štěrba J.,
Grubhoffer L.,
Růžek D. (2016) Expression of a second open reading frame present in the genome of tick-borne encephalitis virus strain Neudoerfl is not detectable in infected cells Virus Genes
52
: 309-316. DOI: 10.1007/s11262-015-1273-y |
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Dzyuba V., Słowińska M., Cosson J., Ciereszko A., Boryshpolets S., Štěrba J., Rodina M., Linhart O., Dzyuba B. (2016) Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation Fish Physiology and Biochemistry
42
: 1755-1766. DOI: 10.1007/s10695-016-0255-x |
