RNDr. Ján Štěrba, Ph.D.
Job Position: PostDoc - Laboratory of Molecular Ecology of Vectors and Pathogens
Phone:
+420 387776220
E-mail:
sterbaj@paru.cas.cz
Room:
PrF C 01 008
Postdoctoral research fellow
Institute of Parasitology
Biology Centre of the Academy of Sciences of the Czech Republic
&
Assistant professor, Deputy head
Institute of Chemistry and Biochemistry
Faculty of Science, University of South Bohemia
Research interests:
Glycobiology of ticks and tick-borne pathogens, allergenogenic arthropod glycans, mass spectrometry, bioorthogonal chemistry
Education and employment:
2002: BSc. in Chemistry, Faculty of Sciences, University of P. J. Šafárik, Košice, Slovak Republic
2003: studies at Laboratory of Biochemistry, Faculty of Chemistry, Aristoteles University in Thessaloniki within Socrates-Erasmus exchange program
2004: graduated with honors from chemistry (specialization biochemistry), Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, University of P. J. Šafárik, Košice, Slovak Republic; award of the dean of Faculty of Natural Sciences, University of P. J. Šafárik.
2004: Laboratory of Clinical Biochemistry, Health Centre Veľké Kapušany, Veľké Kapušany, Slovak Republic
2004-2012: Ph.D. studies in Molecular and Cellular Biology and Genetics at the Faculty of Biological Sciences (now Faculty of Science), University of South Bohemia, České Budějovice, Czech Republic
2004-2006: research assistant, Biology Centre of Academy of Sciences of the Czech Republic v.v.i., Institute of Parasitology, České Budějovice, Czech Republic
2006: assistant at Faculty of Biological Sciences, USB, České Budějovice
2006: visiting scientist, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada
2007-: assistant professor at Faculty of Biological Sciences, USB, České Budějovice; now Faculty of Science, USB, České Budějovice
2007: visiting scientist, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada
2009, 2010: visiting scientist, National Center for Glycomics and Glycoproteomics, Department of Chemistry, Indiana University, Bloomington, Indiana, USA
2010-: postdoctoral research fellow, Institute of Parasitology, Biology Centre of ASCR, České Budějovice
2014/2015: research stay, Genomics group, SaBio (Sanidad y Biotecnología) research group, National Wildlife Research Institute IREC (CSIC-Universidad de Castilla – La Mancha), Ciudad Real, Spain.
Teaching(at Faculty of Science, University of South Bohemia):
Pharmacology and Toxicology (lectures + practical course, in Czech) and Introduction to Toxicology (lectures + practical course, in Czech);
Xenobiochemistry and Toxicology (lectures + practical course, in English);
Laboratory Course in Biochemistry (in Czech and in English), and Adnavced Course in Bicohemistry (in Czech and in English);
supervisor and co-supervisor of 20 bachelor and masters students, co-supervisor of a student from University of Glasgow (work placement at Faculty of Science, University of South Bohemia)
Published scientific papers:
Růžek D., Kopecký J., Štěrba J., Golovchenko M., Rudenko N., Grubhoffer L.: Non-virulent strains of TBE virus circulating in the Czech Republic. Journal of Clinical Virology 36 (suppl. 2): S41, 2006 (P117).
Růžek D., Štěrba J., Kopecký J., Grubhoffer L.: The supposedly attenuated Hy-HK variant of highly virulent Hypr strain of tick-borne encephalitis virus is obviously a stain of Langat virus. Acta Virologica 50(4), 227-278, 2006.
Štěrba J., Vancová M., Rudenko N., Golovchenko M., Tremblay T.-L., Kelly J.F., MacKenzie R.C., Logan S.M., Grubhoffer L.: Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated. Journal of Bacteriology 190: 2619-2623, 2008.
Man P., Kovář V., Štěrba J., Strohalm M., Kavan D., Kopáček P., Grubhoffer L., Havlíček V.: Deciphering Dorin M glycosylation by mass spectrometry. European Journal of Mass Spectrometry 14: 345-354, 2008.
Štěrba J., Dupejová J., Fišer M., Golovchenko M., Rudenko N., Grubhoffer L.: Identification and characterisation of lectins in several tick species. FEBS Journal, 276 (Suppl. 1): 88, 2009.
Grubhoffer L., Hajdušek O., Vancová M., Štěrba J., Rudenko N.: Glycobiochemistry of ticks, vectors of infectious diseases: carbohydrate-binding proteins and glycans. FEBS Journal, 276 (Suppl. 1): 141, 2009.
Dupejova J., Sterba J., Vancova M., Grubhoffer L.: Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization. Parasites&Vectors, 4:4, 2011.
Sterba J., Dupejova J., Fiser M., Vancova M., Grubhoffer L.: Fibrinogen-related proteins in ixodid ticks. Parasites&Vectors, 4:127, 2011.
Vancova M., Sterba J., Dupejova J., Simonova Z., Nebesarova J., Novotny M.V., Grubhoffer L.: Uptake and incorporation of sialic acid by the tick Ixodes ricinus. Journal of Insect Physiology, 2012, 58, 1277-1287.
Grubhoffer L., Rudenko N., Vancova M., Golovchenko M., Sterba J.: Circulatory system and hemolymph. In: Biology of ticks. Vol. 1. Daniel E. Sonenshine (Editor), R. Michael Roe (Editor). Oxford University Press Inc; 2013, 2nd Revised edition. pp. 258-286.
Sterba J., Vancova M., Sterbova J., Bell-Sakyi L., Grubhoffer L.: The majority of sialylated glycoproteins in adult Ixodes ricinus ticks originate in the host, not the tick. Carbohydr Res, 2014, 389, 93-99.
Hönig V, Svec P, Halas P, Vavruskova Z, Tykalova H, Kilian P, Vetiskova V, Dornakova V, Sterbova J, Simonova Z, Erhart J, Sterba J, Golovchenko M, Rudenko N, Grubhoffer L.: Ticks and tick-borne pathogens in South Bohemia (Czech Republic) - Spatial variability in Ixodes ricinus abundance, Borrelia burgdorferi and tick-borne encephalitis virus prevalence. Ticks Tick Borne Dis, 2015, in press.
Attended conferences:
more than 25 poster presentations and 6 lectures on international conferences
Projects:
2006: Glycosylation of tick-borne encephalitis virus envelope protein and its influence on virus virulence – Grantová agentura JU (PI),
2007: Glycosylation of membrane proteins of Lyme borreliosis spirochetes – Grantová agentura JU (PI),
2009-2011: Glycoprotein analysis of Ixodes ricinus salivary glands – GA AVČR (PI: Dr. Marie Vancová, Institute of Parasitology, BC ASCR, České Budějovice, co-PI: Ján Štěrba, Faculty of Science, University of South Bohemia, České Budějovice),
2016-2022: Research infrastructure C4SYS – MŠMT (PI: R. Ettrich, J. Štěrba – head of one of the research units, member of the Steering Committee).
Team member:
2008-2010: Molecular pathogenesis of tick-borne encephalitis – GAČR,
2009-2011: Interaction of Lyme borreliosis spirochetes and salivary glands of Ixodes ricinus tick: the role of borrelia OspC protein – GAČR,
2005-2011: Centre of molecular ecology of vectors and pathogens – MŠMT,
2010: Distribution and diversity of B. bissettii in the southeastern USA and in Europe – U. S. Civilian Research & Development Foundation,
2015-2017: Novel functions of viral and cellular proteins in tick-borne encephalitis virus infection – GAČR.
