RNDr. Helena Langhansová, Ph.D.

Job Position: Postdoc - Laboratory of Arbovirology

Contact details

Phone: +420 387775463
E-mail: helca@paru.cas.cz

Curriculum vitae

Education

Ph.D. Biology 2007 – 2011; University of South Bohemia, Faculty of Science, Field of Molecular and Cell Biology and Genetics; PhD. thesis: The effect of tick saliva factors on the transmission of Borrelia burgdorferi spirochetes and host immune response
M.Sc. Biology 2004 – 2007; University of South Bohemia, Biological Faculty, Field of Clinical Biology; Master thesis: The real-time PCR usage in monitoring the saliva-activated transmission of Borrelia spirochetes
B.Sc. Biology 2001 – 2004; University of South Bohemia, Biological Faculty, Field of Biomedical Laboratory Techniques; Bachelor thesis: Role of T cell subpopulations during infection with the microsporidian Encephalitozoon cuniculi

Work experiences

Postdoc 2011–now; Biology Centre, Academy of the Sciences of the Czech Republic, Institute of Parasitology, Dept. of Parasite Immunology
Research assistant 2007 – 2011; Biology Centre, Academy of the Sciences of the Czech Republic, Institute of Parasitology, Dept. of Parasite Immunology (part-time job)
Laboratory worker 2004 – 2007; Biology Centre, Academy of the Sciences of the Czech Republic, Institute of Parasitology, Dept. of Parasite Immunology (part-time job)

Practical experiences
Laboratory skills: in vivo experiments with laboratory mice (experimental infection, immunisation, harvesting of blood, bone marrow and tissue samples, manipulation in isolators), tick processing (collecting, artificial infection, saliva and salivary gland extract, experimental feeding), nucleic acid isolation, conventional and real-time PCR, ex vivo cell cultures (lymphocytes, mast cells, dendritic cells, neutrophils, bone marrow), specific antibody and cytokine detection (ELISA, immunofluorescence), mast cell degranulation, immunomagnetic separation of cell subsets, flow cytometry analysis

Conferences

Horká H., Černá K., Skallová A., Kopecký J.: Tick saliva affects both proliferation and distribution of Lyme disease spirochetes in mouse organs (poster). VI. International Conference on Ticks and Tick-borne Pathogens, Buenos Aires, Argentina, September 21-26, 2008.
Horká H., Kýčková K., Fišerová L., Kopecký J.: Experimental infection of Ixodes ricinus ticks with Borrelia burgdorferi spirochetes (poster). IX. International Jena Symposium on Tick-borne Diseases, Jena, Germany, March 15-17, 2007.
Kýčková K., Horká H., Skallová A., Kopecký J.: The effect of tick saliva on the distribution and proliferation of Borrelia spirochetes in murine tissues (poster). IX. International Jena Symposium on Tick-borne Diseases, Jena, Germany, March 15-17, 2007.
Salát J., Horká H., Kopecký J.: Role of T cell subsets in the immune response against Encephalitozoon cuniculi infection (poster). Emergent Pathogens in the 21st Century: First United Workshop on Microsporidia from Invertebrate and Vertebrate Hosts, České Budějovice, Czech Republic, July 12-15, 2004.

Training programs

Universitätsmedizin der Johanes Guttenberg-Universität, Institut für Immunologie, Mainz, Germany, October-November, 2009.

Courses

Summer School on Vector Molecular Biology, České Budějovice, Czech Republic, June 20-24, 2005.
Certificate of competency according to § 17 of the Act No. 246/1992 coll. on Protection Animals against Cruelty in present statues at large. University of Veterinary and Pharmaceutical Sciences Brno and Central commission for Animal Welfare (CCAW), 2005.

Additional Skills

Languages - English (upper intermediate), German (elementary), Spanish (elementary)
Computer skills - www, MS Office, CorelDRAW
Driving skills - passenger car

Publications

Total found: 19 records
Frutos E. , Karlík M. , Jiménez J., Langhansová H., Lieskovská J., Polcar T. (2018) Development of new beta/alpha ''-Ti-Nb-Zr biocompatible coating with low Young's modulus and high toughness for medical applications Materials and Design 142 : 44–55. DOI: 10.1016/j.matdes.2018.01.014Get
Kratochvíl J., Kahoun D., Štěrba J., Langhansová H., Lieskovská J., Fojtíková P., Hanuš J., Kousal J., Kylián O., Straňák V. (2018) Plasma polymerized C:H:N:O thin films for controlled release of antibiotic substances Plasma Processes and Polymers 15 : 1700160. DOI: 10.1002/ppap.201700160
Kratochvíl J., Štěrba J., Lieskovská J., Langhansová H., Kuzminova A., Khalakhan I., Kylián O., Straňák V. (2018) Antibacterial effect of Cu/C:F nanocomposites deposited on PEEK substrates Materials Letters 230 : 96–99. DOI: 10.1016/j.matlet.2018.07.082
Lieskovská J., Páleníková J., Langhansová H., Chmelař J., Kopecký J. (2018) Saliva of Ixodes ricinus enhances TBE virus replication in dendritic cells by modulation of pro-survival Akt pathway Virology 514 : 98–105. DOI: 10.1016/j.virol.2017.11.008
Širmarová J., Salát J., Palus M., Hönig V., Langhansová H., Holbrook M., Růžek D. (2018) Kyasanur Forest disease virus infection activates human vascular endothelial cells and monocyte-derived dendritic cells Emerging Microbes & Infections 7 : 175. DOI: 10.1038/s41426-018-0177-z
Chmelař J., Kotál J., Langhansová H., Kotsyfakis M. (2017) Protease inhibitors in tick saliva: the role of serpins and cystatins in tick-host-pathogen interaction Frontiers in Cellular and Infection Microbiology 7 : 216. DOI: 10.3389/fcimb.2017.00216
Klein M., Brühl T., Staudt V., Reuter S., Grebe N., Gerlitzki B., Hoffmann M., Bohn T., Ulges A., Stergiou N., De Graaf J., Löwer M., Taube C., Becker M., Hain T., Dietzen S., Stassen M., Huber M., Lohoff M., Campos Chagas A., Andersen J., Kotál J., Langhansová H., Kopecký J., Schild H., Kotsyfakis M., Schmitt E., Bopp T. (2015) Tick Salivary Sialostatin L Represses the Initiation of Immune Responses by Targeting IRF4-Dependent Transcription in Murine Mast Cells The Journal of Immunology 195 : 621-631. DOI: 10.4049/jimmunol.1401823
Kotál J., Langhansová H., Lieskovská J., Andersen J., Francischetti I., Chavakis T., Kopecký J., Pedra J., Kotsyfakis M., Chmelař J. (2015) Modulation of host immunity by tick saliva Journal of Proteomics 128 : 58-68. DOI: 10.1016/j.jprot.2015.07.005
Lieskovská J., Páleníková J., Langhansová H., Chagas A., Calvo E., Kotsyfakis M., Kopecký J. (2015) Tick sialostatins L and L2 differentially influence dendritic cell responses to Borrelia spirochetes Parasites & Vectors 8 : 275. DOI: 10.1186/s13071-015-0887-1
Páleníková J., Lieskovská J., Langhansová H., Kotsyfakis M., Chmelař J., Kopecký J. (2015) Ixodes ricinus Salivary Serpin IRS-2 Affects Th17 Differentiation via Inhibition of the Interleukin-6/STAT-3 Signaling Pathway Infection and Immunity 83 : 1949-1956. DOI: 10.1128/IAI.03065-14
Palus M., Bílý T., Elsterová J., Langhansová H., Salát J., Vancová M., Růžek D. (2014) Infection and injury of human astrocytes by tick-borne encephalitis virus JOURNAL OF GENERAL VIROLOGY 95 : 2411-2426. DOI: 10.1099/vir.0.068411-0
Horká H., Staudt V., Klein M., Taube C., Reuter S., Dehzad N., Andersen J., Kopecký J., Schild H., Kotsyfakis M., Hoffmann M., Gerlitzki B., Stassen M., Bopp T., Schmitt E. (2012) The tick salivary protein Sialostatin L inhibits the Th9-derived production of the asthma-promoting cytokine IL-9 and is effective in the prevention of experimental asthma. Journal of Immunology 188 : 2669–2676. DOI: 10.4049/jimmunol.1100529
Kotsyfakis M., Horká H., Salát J., Andersen J. (2010) The crystal structures of two salivary cystatins from the tick Ixodes scapularis and the effect of these inhibitors on the establishment of Borrelia burgdorferi infection in a murine model. Molecular Microbiology 77 : 456–470. DOI: 10.1111/j.1365-2958.2010.07220.x
Salát J., Paesen G., Řezáčová P., Kotsyfakis M., Kovářová Z., Šanda M., Majtán J., Grunclová L., Horká H., Andersen J., Brynda J., Horn M., Nunn M., Kopáček P., Kopecký J., Mareš M. (2010) Crystal structure and functional characterization of an immunomodulatory salivary cystatin from the soft tick Ornithodoros moubata. Biochemical Journal 429 : 103–112. DOI: 10.1042/BJ20100280
Horká H., Černá-Kýčková K., Skallová A., Kopecký J. (2009) Tick saliva affects both proliferation and distribution of Borrelia burgdorferi spirochetes in mouse organs and increases transmission of spirochetes to ticks. International Journal of Medical Microbiology 299 : 373-380. DOI: 10.1016/j.ijmm.2008.10.009
Fišerová L., Černá K., Horká H., Kopecký J. (2008) Two ways of experimental infection of Ixodes ricinus ticks (Acari: Ixodidae) with spirochetes of Borrelia burgdorferi sensu lato complex. Folia Parasitologica 55 : 150-154. Documents to download: 2008_Fiserova_Fp_150 (pdf)
Sak B., Salát J., Horká H., Saková K., Ditrich O. (2006) Antibodies enhance the protective effect of CD4+ T lymphocytes in SCID mice perorally infected with Encephalitozoon cuniculi. Parasite Immunology 28 : 95-99. DOI: 10.1111/j.1365-3024.2005.00813.x
Salát J., Horká H., Sak B., Kopecký J. (2006) Pure CD4+ T lymphocytes fail to protect perorally infected SCID mice from lethal microsporidiosis caused by Encephalitozoon cuniculi. Parasitology Research 99 : 682-686. DOI: 10.1007/s00436-006-0208-x
Severinová J., Salát J., Kročová Z., Řezníčková J., Demová H., Horká H., Kopecký J. (2005) Coinoculation of Borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice. Folia Microbiologica 50 : 457–463. DOI: 10.1007/BF02931430

RNDr. Helena Langhansová, Ph.D.

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