RNDr. Helena Langhansová, Ph.D.
Job Position: Postdoc - Laboratory of Arbovirology
- Ph.D. Biology 2007 – 2011; University of South Bohemia, Faculty of Science, Field of Molecular and Cell Biology and Genetics; PhD. thesis: The effect of tick saliva factors on the transmission of Borrelia burgdorferi spirochetes and host immune response
- M.Sc. Biology 2004 – 2007; University of South Bohemia, Biological Faculty, Field of Clinical Biology; Master thesis: The real-time PCR usage in monitoring the saliva-activated transmission of Borrelia spirochetes
- B.Sc. Biology 2001 – 2004; University of South Bohemia, Biological Faculty, Field of Biomedical Laboratory Techniques; Bachelor thesis: Role of T cell subpopulations during infection with the microsporidian Encephalitozoon cuniculi
- Postdoc 2011–now; Biology Centre, Academy of the Sciences of the Czech Republic, Institute of Parasitology, Dept. of Parasite Immunology
- Research assistant 2007 – 2011; Biology Centre, Academy of the Sciences of the Czech Republic, Institute of Parasitology, Dept. of Parasite Immunology (part-time job)
- Laboratory worker 2004 – 2007; Biology Centre, Academy of the Sciences of the Czech Republic, Institute of Parasitology, Dept. of Parasite Immunology (part-time job)
Laboratory skills: in vivo experiments with laboratory mice (experimental infection, immunisation, harvesting of blood, bone marrow and tissue samples, manipulation in isolators), tick processing (collecting, artificial infection, saliva and salivary gland extract, experimental feeding), nucleic acid isolation, conventional and real-time PCR, ex vivo cell cultures (lymphocytes, mast cells, dendritic cells, neutrophils, bone marrow), specific antibody and cytokine detection (ELISA, immunofluorescence), mast cell degranulation, immunomagnetic separation of cell subsets, flow cytometry analysis
- Horká H., Černá K., Skallová A., Kopecký J.: Tick saliva affects both proliferation and distribution of Lyme disease spirochetes in mouse organs (poster). VI. International Conference on Ticks and Tick-borne Pathogens, Buenos Aires, Argentina, September 21-26, 2008.
- Horká H., Kýčková K., Fišerová L., Kopecký J.: Experimental infection of Ixodes ricinus ticks with Borrelia burgdorferi spirochetes (poster). IX. International Jena Symposium on Tick-borne Diseases, Jena, Germany, March 15-17, 2007.
- Kýčková K., Horká H., Skallová A., Kopecký J.: The effect of tick saliva on the distribution and proliferation of Borrelia spirochetes in murine tissues (poster). IX. International Jena Symposium on Tick-borne Diseases, Jena, Germany, March 15-17, 2007.
- Salát J., Horká H., Kopecký J.: Role of T cell subsets in the immune response against Encephalitozoon cuniculi infection (poster). Emergent Pathogens in the 21st Century: First United Workshop on Microsporidia from Invertebrate and Vertebrate Hosts, České Budějovice, Czech Republic, July 12-15, 2004.
- Universitätsmedizin der Johanes Guttenberg-Universität, Institut für Immunologie, Mainz, Germany, October-November, 2009.
- Summer School on Vector Molecular Biology, České Budějovice, Czech Republic, June 20-24, 2005.
- Certificate of competency according to § 17 of the Act No. 246/1992 coll. on Protection Animals against Cruelty in present statues at large. University of Veterinary and Pharmaceutical Sciences Brno and Central commission for Animal Welfare (CCAW), 2005.
- Languages - English (upper intermediate), German (elementary), Spanish (elementary)
- Computer skills - www, MS Office, CorelDRAW
- Driving skills - passenger car