Dr. Michail Kotsyfakis, Ph.D.

Job Position: Head of Laboratory - Laboratory of Genomics and Proteomics of Disease Vectors

Contact details

Phone: +420 387775492
E-mail: kotsyfakis@paru.cas.cz
Room: 232

Curriculum vitae

I.  CURRICULUM VITAE

Personal details

Date and place of birth -20/01/1976 - Athens, Greece
Family status - Married (father of a son)

 

Current Role

I am an established group leader at the front-line of systems biology research into arthropod disease vectors. My current research focuses on understanding and using arthropod vectors as a model to address two fundamental practical problems: how to combat the public health threat posed by the pathogens carried by these vectors and how the mechanisms adopted by vectors can be translated into treatments for human diseases. This focus has enabled me to establish a strong publication record, attract external funding, and recruit high-quality post-doctoral scientists from abroad. I am an enthusiastic, thoughtful, and supportive teacher, advisor, and mentor to students and group members, and have a particular interest in mentoring and retaining young scientific talent.

 

Employment history

9/2009 – present: Principal Investigator (currently at Grade 5, equivalent to Full Professor) at the Laboratory of Genomics and Proteomics of Disease Vectors, Biology Centre, Czech Academy of Sciences, Czechia.  
3/2005 - 9/2009Post-doctoral research associate at the Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA.
Mentor: Professor José M. C. Ribeiro
12/2004 - 3/2005Post-doctoral fellow at the Laboratory of Insect Molecular GeneticsInstitute of Molecular Biology and Biotechnology, Foundation of Research and Technology - Hellas (F.O.R.T.H.), Heraklion Crete, Greece.
Mentor: Professor Christos (Kitsos) Louis

 

Academic record

11/2000 - 12/2004Ph.D. in Biology, Joint Graduate Program of the Departments of Medicine and Biology, University of Crete, and the Institute of Molecular Biology and Biotechnology, F.O.R.T.H., Heraklion Crete, Greece.
Thesis title: A study of the Anopheles annexin family as proteins that bind Plasmodium berghei parasites
(2004, University of Crete)
Ph.D. Advisor: Professor Christos (Kitsos) Louis

11/1998 - 11/2000M.Sc. in Molecular Biology and Biomedicine, Joint Graduate Program of the Departments of Medicine and Biology, University of Crete, and the Institute of Molecular Biology and Biotechnology, F.O.R.T.H., Heraklion Crete, Greece.
Thesis title: Studies on the interaction of Plasmodium parasites with Anopheles mosquitoes
(2000, University of Crete)
M.Sc. Advisor: Professor Christos (Kitsos) Louis

10/1994 - 11/1998B.Sc. in Biology, University of Crete, Heraklion Crete, Greece.

 

Current team composition 

– Engineers and technicians
KREMLOVA, Marketa MSc (full-time contractor)
ERHART, Jan MSc (50% part-time contractor)

- Ph.D. student
KOTAL, Jan MSc

- Post-doc
MARTINS, Larissa PhD (since March 19th 2019, country of origin Brazil)
BENSAOUD, Chaima PhD (since June 11th 2019, country of origin Tunisia)

 

Grants (since group establishment in 2009)

Selected oral presentations/talks at international conferences
(last 5 years, 41 throughout entire career)

- Annual meeting of the American Society for Exosomes and Microvesicles, 20-24 October 2018, Baltimore, USA (invited speaker).

Invited talk at the Institute of Molecular Biology and Biotechnology, 1 December 2017, Heracklion-Crete, Greece.

- 25th International Congress of Entomology, 25-30 September 2016, Orlando, USA (invited speaker).

- Joint Dutch/Belgian Parasitology Society Spring meeting, 20 May 2016, Rotterdam, The Netherlands (keynote speaker).

- 10th European Congress of Entomology, 3-8 August 2014, York, United Kingdom (invited speaker).

- National Institute on Aging, National Institutes of Health, 19 May 2014, Baltimore, USA (invited seminar).

- 8th General Meeting of the International Proteolysis Society, 20-24 October 2013, Cape Town, South Africa.

- EMBO Conference: Molecular and Population Biology of Mosquitoes and Other Disease Vectors: From Basic Vector Biology to Disease Control, 15-19 July 2013, Kolymbari, Crete, Greece.

- 5th International Conference on Exogenous Factors Affecting Thrombosis and Haemostasis, 5-6 July 2013, Amsterdam, The Netherlands (invited speaker).

 

On-going collaborations
(alphabetically)

Triantafyllos Chavakis, Professor and Division Chief, University Clinic Carl Gustav Carus, Dresden University of Technology, Dresden, Germany.
Michael Hackenberg, Assistant Professor, Dpto. de Genética, Facultad de Ciencias, Campus Fuentenueva s/n, Universidad de Granada, Granada, Spain.Joao H Pedra, Assistant Professor at the Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, USA.
José MC Ribeiro, Head of the Vector Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, USA.
Edgar Schmitt, Professor, Institute of Immunology, Johannes Gutenberg University of MainzMainz, Germany.
Stefan Tenzer, Head of Mass-Spec Core Facility, Johannes Gutenberg University of Mainz, Mainz, Germany.

 

Fellowships / Research abroad

10/2013EMBO laboratory management course for group leaders, Leimen, Germany.

8/2013: Research stay in the Department of Internal Medicine, University Clinic Carl Gustav Carus, Dresden University of Technology, Dresden, Germany (Laboratory head: Professor Triantafyllos Chavakis).

3/2013 - 4/2013, 9/2013EMBO Short-Term Fellowship (ASTF number 482-2012). 7,200 Euros in total. This fellowship supported a visit as a Special Volunteer to the Laboratory of Malaria & Vector Research, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Rockville, USA (Laboratory head: Professor Jose' MC Ribeiro). 

2/2010 - 2/2012Alexander von Humboldt fellowship to Dr Alexandra Schwarz to join my group as a post-doctoral fellow (host laboratory). 26,000 Euros per year, 52,000 Euros in total. This fellowship supported the stay of Dr. Schwarz as a post-doctoral fellow in my laboratory at the Biology Centre, Academy of Sciences of the Czech Republic, Budweis, Czech Republic.

9/2009 - 8/2014Jan Evangelista Purkyně Fellowship from the Czech Academy of Sciences (the first to receive the fellowship in South Bohemia and the first non-Czech speaker). 30,000 Euros per year, 150,000 Euros in total. The fellowship supported my salary as a group leader at the Biology Centre, Czech Academy of Sciences, Budweis, Czech Republic.

3/2005 - 10/2009Post-doctoral Visiting Fellowship, Laboratory of Malaria & Vector Research, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Rockville, USA. (Intramural grant number 0012117069). 45,000 US dollars per year, ~ 200,000 US dollars in total.

6/2002 - 8/2002EMBO Short-Term Fellowship (ASTF number 67-2002). The fellowship supported my stay as a graduate student in the Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France under the directorship of the Nobel laureate Professor Jules Hoffmann.

 

Memberships and awards

4/2016President’s award from the Grant Agency of the Czech Republic for excellent research performance.

10/2005 - presentInternational Proteolysis Society Member.

1/2008: American Society for Biochemistry and Molecular Biology Post-doctoral Travel Award to participate in the 2008 society meeting in San Diego, USA.

11/2007 - presentAmerican Society for Biochemistry and Molecular Biology Member (ID: 23561).

9/2007NIH/USA Fellows Award for Research Excellence in Biomedical Research.

8/2007: International Proteolysis Society (IPS) travel award to participate in the 5th IPS meeting in Patras, Greece.

1/2007: Young Scientist Award to participate in the 10th International Union of Biochemistry and Molecular Biology/Sociedade Brasileira de Bioquimica e Biologia (IUBMB/SBBq) meeting in Salvador, Brazil.

9/2006NIH/USA Fellows Award for Research Excellence in Biomedical Research.

8/2005: International Proteolysis Society travel award to participate in the 4th IPS meeting in Quebec, Canada.

 

Scientific reviewer 

Associate editor for: BMC Genomics [I.F.=3.5; (12/2014-to date: 50 manuscripts edited)], American Journal of Blood Research (02/2013-to date) and Parasites&Vectors [I.F.=3.16 at the time I stopped; (1/2016-12/2018: 25 manuscripts edited)].

External grant reviewer for: France’s Agence Nationale de la Recherche (ANR), the Swiss Secretariat for Education, Research and Innovation (SERI), the Research Foundation Flanders (FWO), the National Science Centre of Poland (NCN), and the Slovak Research and Development Agency (APVV), CINECA consortium of Italian Universities.

External reviewer for: Blood, PNAS, Trends in Biochemical Sciences, Trends in Parasitology, Journal of Proteome Research, Scientific Reports, PLoS Neglected Tropical Diseases, BMC Genomics, Journal of Proteomics, The FEBS Journal, FEBS Letters, Developmental & Comparative Immunology, PLoS One, Insect Biochemistry and Molecular Biology, Infection Genetics and Evolution, International Journal for Parasitology, Parasites and Vectors, Insect Molecular Biology, Biochimie, Peptides, Ticks and Tick-borne Diseases, Gene, Veterinary Parasitology, Expert review of Proteomics, Pest Management Science, Pesticide Biochemistry and Physiology, Sensors, Parasite, Physiological Entomology.

 

Teaching and supervisory experience (please also see the table below)

06/2019affiliation with the Faculty of Science, University of South Bohemia, Budweis, Czechia.

05, 06/2019: Supervision of Ms. Magdalena Szczotko as a graduate Erasmus visiting student.

10/2012 - 9/2015: Undergraduate student supervision (Ms. Lovelyna Eromonsele), Faculty of Science, University of South Bohemia, Budweis, Czechia.

10/2011 - 1/2014: M.Sc. supervision (Mr. Jan Kotal), Faculty of Science, University of South Bohemia, Budweis, Czechia.

09/2011 and every winter semester until 2018: Teaching ‘The Human Plasma Proteome’ course (course leader, affiliation with the Faculty of Science, University of South Bohemia, Budweis, Czechia).

1/2011 - present: Reviewer and committee member for graduate and undergraduate students of the Faculty of Science, University of South Bohemia, Budweis, Czechia.

09/2010 - 01/2011: Teaching Molecular and Biochemical Parasitology, Faculty of Science, University of South Bohemia, Budweis, Czechia.

10/2009 - 6/2011: Undergraduate supervision (Ms. Barbora Singerova and Mr. Jan Kotal) at the Faculty of Science, University of South Bohemia, Budweis, Czechia.

 

Previous PhD students

Previous PhD students

Number of Publications

Name

Start Date

Defence Date

1st author

Co-author

Jindrich Chmelar (co-supervisor)

January 2008

November 2010

2

2

Helena Horka (co-supervisor)

January 2009

May 2011

2

2

 

Current PhD student

PhD student Name

Start Date

Planned End Date

Number of Publications

1st author

Co-author

Jan Kotal

February 2014

December 2019

3

6

 

Post-Doc mentoring (1st author, co-authoring)

Post-Doc Name

Year of PhD

Start Date

End Date

 

Number of Publications

1st author

Co-author

Jindrich Chmelar (20% part time)

 

2010

 

December 2010

 

December 2013

 

2

-

Alexandra Schwarz

(coming from Germany)

2009

February 2010

February 2014

6

3

James Valdes

(coming from the USA)

2009

August 2011

September 2012

1

3

 

Honours 

● I was awarded the NIH Fellows Award for Research Excellence in Biomedical Research for two consecutive years, which places me in the top 10% of NIH intramural research fellows. I have twice been supported by an EMBO-Short-Term fellowship and selected to attend the EMBO Laboratory management course for group leaders.

● I was the first non-Czechoslovakian honoured with a Jan Evangelista Purkyně Fellowship, established by the Czech Academy of Sciences to facilitate the recruitment and integration of recognised senior investigators in the national research infrastructure. This was the first fellowship awarded to an individual at a research institution outside Prague and to a non-Czech speaker, highlighting the recognition by the Czech academia of my scientific contribution.

● The completion of my first research grant from the Grant Agency of the Czech Republic (the main independent funding agency in the Czech Republic) resulted in an award from the President of the Agency for excellent research performance (we were among the top three teams in research outcome, at the national level, in all disciplines).

● I am an internationally recognised researcher at the front-line of my research field. This has enabled me to recruit high-quality post-doctoral scientists from the USA and Germany, including one sponsored by a prestigious Alexander von Humboldt Fellowship (usually awarded to attract a scientist in Germany).

 

II. PUBLICATION LIST (Journal Impact Factor for 2018 from Web of Science, ORCID: 0000-0002-7526-1876)

IIA. 15 most significant publications 
(in chronological order out of 63 total publications,
3 book chapters and 3 patents, underlined the 5 most significant)

15. Bensaoud C, Hackenberg M, Kotsyfakis M (2019). Noncoding RNAs in parasite-vector-host interactions. (Most read article in Trends in ParasitologyTrends Parasitol. 35(9):715-724.     (I.F.: 8)

14. Kotál J, Stergiou N, Buša M, Chlastáková A, Beránková Z, Řezáčová P, Langhansová H, Schwarz A, Calvo E, Kopecký J, Mareš M, Schmitt E, Chmelař J, Kotsyfakis M (2019). The structure and function of Iristatin, a novel immunosuppressive tick salivary cystatin. Cell Mol Life Sci. 76(10):2003-2013.     (I.F.: 7)

13. Hackenberg M, Kotsyfakis M (2018). Exosome-mediated pathogen transmission by arthropod vectors. Trends Parasitol. 34(7):549-552.     (I.F.: 8)

12. Wang X, Shaw DK, Hammond HL, Sutterwala FS, Rayamajhi M, Shirey KA, Perkins DJ, Bonventre JV, Velayutham TS, Evans SM, Rodino KG, VieBrock L, Scanlon KM, Carbonetti NH, Carlyon JA, Miao EA, McBride JW, Kotsyfakis M, Pedra JHF (2016).  The prostaglandin E2-EP3 receptor axis regulates Anaplasma phagocytophilum-mediated NLRC4 inflammasome activation. PLoS Pathogens 12(8):e1005803.     (I.F.: 6.5)

11. Chmelař J, Kotál J, Kopecky J, Pedra JH, Kotsyfakis M (2016). All for οne and οne for all on the tick-host battlefield. Trends Parasitol. 32(5):368-377.     (I.F.: 8)

10. Chmelař J, Kotál J, Karim S, Kopacek P, Francischetti IMB, Pedra JH, Kotsyfakis M (2016). Sialomes and mialomes: a systems biology view of tick tissues and tick-host interactions. Trends Parasitol. 32(3):242-254.     (I.F.: 8)

 9. Kotsyfakis M, Kopacek P, Franta Z, Pedra JHF, Ribeiro JMC (2015). Deep sequencing analysis of the Ixodes ricinus haemocytome. PLoS Negl Trop Dis., 9(5):e0003754.     (I.F.:  4.5)

8. Schwarz A, Tenzer S, Hackenberg M, Erhart J, Gerhold-Ay A, Mazur J, Kuharev J, Ribeiro JMC, Kotsyfakis M (2014). A systems level analysis reveals transcriptomic and proteomic complexity in Ixodes ricinus midgut and salivary glands during early attachment and feeding. Molecular and Cellular Proteomics, 13(10):2725-2735.     (I.F.: 4.8)

7. Ma D, Mizurini DM, Assumpção TCF, Li Y, Qi Y, Kotsyfakis M, Ribeiro JMC, Monteiro RQ, Francischetti IMB (2013). Desmolaris, a novel Factor XIa anticoagulant from the salivary gland of the vampire bat (Desmodus rotundus) inhibits inflammation and  hrombosis. Blood 122(25):4094-4106.     (I.F.: 16.6)

6. Schwarz A, von Reumont BM, Erhart J, Chagas AC, Ribeiro JMC, Kotsyfakis M (2013). De novo Ixodes ricinus salivary transcriptome analysis using two different next generation sequencing methodologies. The FASEB Journal 27(12):4745-4756.     (I.F.: 5.4)

5. Chmelar J, Oliveira CJ, Rezacova P, Francischetti IMB, Kovarova Z, Pejler G, Kopacek P, Ribeiro JMC, Mares M, Kopecky J and Kotsyfakis M (2011). A tick salivary protein targets cathepsin G and chymase and inhibits host inflammation and platelet aggregation. Blood 117(3):736-744. Cover of the issue.     (I.F.: 16.6)

4.  Sá-Nunes A, Bafica A, Antonelli LRV, Choi EY, Francischetti IMB, Andersen JF, Shi GP, Chavakis T, Ribeiro JMC, Kotsyfakis M (2009). Sialostatin L inhibits antigen‑specific T cell proliferation and ameliorates experimental autoimmune encephalomyelitis. J Immunol. 182(12):7422-7429.     (I.F.: 4.7)

3.  Kotsyfakis M, Anderson JM, Andersen JF, Calvo E, Francischetti IMB, Mather TN, Valenzuela JG, Ribeiro JMC (2008). Immunity against a "silent" salivary antigen of the Lyme vector Ixodes scapularis impairs its ability to feed. J Immunol. 181(8):5209-5212. Cutting Edge Article.     (I.F.: 4.7) 

2Kotsyfakis M, Karim S, Andersen JF, Mather TN, Ribeiro JMC (2007). Selective cysteine protease inhibition contributes to blood feeding success of the tick Ixodes scapularis. J Biol Chem. 282(5):29256-29263.     (I.F.: 4.1)

1. Kotsyfakis M, Sá-Nunes A, Francischetti IMB, Mather TN, Andersen JF, Ribeiro JMC (2006). Antiinflammatory and immunosuppressive activity of sialostatin L, a salivary cystatin from the tick Ixodes scapularis. J Biol Chem. 281(36):26298-26307.     (I.F.: 4.1)


IIB. Full publication list
IIB1) Publication of research articles in international peer-reviewed journals 

Total Impact Factor of research publications: 203.9  
Mean Impact Factor per research publication: 4.5
 

Publication of research articles in international peer-reviewed journals since 2009 (since group establishment in Czech Republic) († corresponding author, * equal contribution, # equal senior author)

45. Kotál J, Stergiou N, Buša M, Chlastáková A, Beránková Z, Řezáčová P, Langhansová H, Schwarz A, Calvo E, Kopecký J, Mareš M, Schmitt E, Chmelař J, Kotsyfakis M† (2019).The structure and function of Iristatin, a novel immunosuppressive tick salivary cystatin. Cell Mol Life Sci. 76(10):2003-2013.     (I.F.: 7)

44. Assumpção TC, Mizurini DM, Ma D, Monteiro RQ, Ahlstedt S, Reyes M, Kotsyfakis M, Mather TN, Andersen JF, Lukszo J, Ribeiro JMC, Francischetti IMB (2018). Ixonnexin from tick saliva promotes fibrinolysis by interacting with plasminogen and tissue-type plasminogen activator, and prevents arterial thrombosis. Sci Rep. 8(1):4806.     (I.F.: 4)

43. Hackenberg M, Langenberger D, Schwarz A, Erhart J, Kotsyfakis M† (2017). In silico target network analysis of de novo-discovered, tick saliva-specific microRNAs reveals important combinatorial effects in their interference with vertebrate host physiology. RNA 23(8):1259-1269.     (I.F.: 3.9)

42. Cabezas-Cruz A, Tonk M, Bouchut A, Pierrot C, Pierce RJ, Kotsyfakis M, Rahnamaeian M, Vilcinskas A, Khalife J, Valdes JJ (2016). Antiplasmodial activity is an ancient and conserved feature of tick defensins. Frontiers in Microbiology 7:1682.     (I.F.: 4.3)

41. Wang X, Shaw DK, Hammond HL, Sutterwala FS, Rayamajhi M, Shirey KA, Perkins DJ, Bonventre JV, Velayutham TS, Evans SM, Rodino KG, VieBrock L, Scanlon KM, Carbonetti NH, Carlyon JA, Miao EA, McBride JW, Kotsyfakis M, Pedra JHF (2016). The prostaglandin E2-EP3 receptor axis regulates Anaplasma phagocytophilum-mediated NLRC4 inflammasome activation. PLoS Pathogens 12(8):e1005803.     (I.F.: 6.5)

40. Wang X, Shaw DK, Sakhon OS, Snyder GA, Sundberg EJ, Santambrogio L, Sutterwala FS, Dumler S, Shirey KA, Perkins DJ, Richard K, Chagas AC, Calvo E, Kopecky J, Kotsyfakis M, Pedra JHF (2016). The tick protein Sialostatin L2 binds to Annexin A2 and inhibits NLRC4-mediated inflammasome activation. Infect Immun. 84(6):1796-1805.     (I.F.: 3.2)

39. Ferreira VP, Vale VF, Pangburn MK, Abdeladhim M, Mendes-Sousa AF, Coutinho-Abreu IV, Rasouli M, Brandt EA, Meneses C, Lima III KF, Araújo RN, Pereira MH, Kotsyfakis M, Oliveira F, Kamhawi S, Ribeiro JMC, Gontijo NF, Collin N, Valenzuela JG (2016). SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis. Sci Rep. 6:19300.     (I.F.: 4)

38. Francischetti IMB, Assumpção TCF, Ma D, Mizurini DM, Kini RM, Ribeiro JMC, Kotsyfakis M, Monteiro RQ (2016). In vitro mode of action and anti-thrombotic activity of Boophilin, a multifunctional Kunitz protease inhibitor from the midgut of a tick vector of Babesiosis, Rhipicephalus microplus. PLoS Neglected Tropical Diseases, 10(1):e0004298.     (I.F.:  4.5)

37. Tonk M, Cabezas-Cruz A, Valdés JJ, Rego RO, Grubhoffer L, Estrada-Peña A, Vilcinskas A, Kotsyfakis M,  Rahnamaeian M (2015). Ixodes ricinus defensins attack distantly-related pathogens. Developmental & Comparative Immunology, 53(2):358-365.     (I.F.: 3.1)

36. Jablonka W, Kotsyfakis M, Mizurini DM, Monteiro RQ, Lukszo J, Drake SK, Ribeiro JMC, Andersen JF (2015). Identification and mechanistic analysis of a novel tick-derived inhibitor of thrombin. PLoS One, 10(8):e0133991.     (I.F.: 2.8)

35. Klein M*, Brühl TJ*, Staudt V*, Reuter S, Grebe N, Gerlitzki B, Hoffmann M, Bohn T, Ulges A, Stergiou N, de Graaf J, Löwer M, Taube C, Becker M, Stassen M, Huber M, Lohoff M, Chagas AC, Andersen JF, Kotal J, Langhansová H, Kopecky J, Schild H, Kotsyfakis M, Schmitt E#, Bopp T# (2015). Tick salivary Sialostatin L represses the initiation of immune responses by targeting IRF4-dependent transcription in murine mast cells.The Journal of Immunology, 195(2):621-631.    (I.F.:  4.7)

34. Lieskovská J, Palenikova J, Langhansova H, Chagas AC, Calvo E, Kotsyfakis M, Kopecky J (2015). Tick sialostatins L and L2 differentially influence dendritic cell responses to Borrelia spirochetes. Parasit Vectors 8(1):275.     (I.F.: 3)

33. Kotsyfakis M*†, Kopacek P*, Franta Z, Pedra JHF, Ribeiro JMC (2015). Deep sequencing analysis of the Ixodes ricinus haemocytome. PLoS Negl Trop Dis., 9(5):e0003754.     (I.F.:  4.5) 

32. Kotsyfakis M†, Schwarz A, Erhart J, Ribeiro JMC (2015). Tissue- and time-dependent transcription in Ixodes ricinus salivary glands and midguts when blood feeding on the vertebrate host. Sci Rep. 5:9103.     (I.F.: 4)

31. Palenikova J, Lieskovska J, Langhansova H, Kotsyfakis M, Chmelar J, Kopecky J (2015).Ixodes ricinus salivary serpin IRS-2 affects Th17 differentiation via inhibition of IL-6/STAT-3 signaling pathway.Infect Immun. 83(5):1949-1956.     (I.F.: 3.2)

30. Lieskovska J, Palenikova J, Sirmarova J, Elsterova J, Kotsyfakis M, Campos Chagas A, Calvo E, Ruzek D, Kopecky J (2015). Tick salivary cystatin sialostatin L2 suppresses IFN responses in mouse dendritic cells. Parasite Immunol. 37(2):70-78.     (I.F.: 2.8)

29. Waisberg M, Molina-Cruz A, Mizurini DM, Gera N, Sousa BC, Ma D, Leal AC, Gomes T, Kotsyfakis M, Ribeiro JMC, Lukszo J, Reiter K, Porcella S, Oliveira CJ, Monteiro RQ, Barillas-Mury, Pierce SK, Francischetti IMB (2014).Plasmodium sp infection induces expression of a mosquito salivary protein (Agaphelin) that targets neutrophil function and inhibits thrombosis without impairing hemostasis.PLoS Pathogens, 10(9):e1004338.     (I.F.: 6.5)

28. Schwarz A*, Tenzer S*, Hackenberg M, Erhart J, Gerhold-Ay A, Mazur J, Kuharev J, Ribeiro JMC#, Kotsyfakis M#† (2014).A systems level analysis reveals transcriptomic and proteomic complexity in Ixodes ricinus midgut and salivary glands during early attachment and feeding.Molecular and Cellular Proteomics, 13(10):2725-2735.     (I.F.: 4.8)

27. Chen G*, Wang X*, Severo MS, Sakhon OS, Sohail M, Brown L, Sircar M, Snyder G, Sundberg E, Ulland TK, Olivier AK, Andersen JF, Zhou Y, Shi GP, Sutterwala FS , Kotsyfakis M, Pedra JHF (2014). Sialostatin L2 inhibits caspase-1-mediated inflammation during Anaplasma phagocytophilum infection. Infect Immun. 82(6):2553-2564. Selected by the editors as a Spotlight of the issue.     (I.F.: 3.2)

26. Bizzarro B, Barros MS, Maciel C, Gueroni DI, Campopiano J, Kotsyfakis M, Amarante-Mendes GP, Calvo E, Capurro ML, Sá-Nunes A (2013).Effects of Aedes aegypti salivary components on dendritic cell and lymphocyte biology.Parasit Vectors 6(1):329.     (I.F.: 3)

25. Ma D, Mizurini DM, Assumpção TCF, Li Y, Qi Y, Kotsyfakis M, Ribeiro JMC, Monteiro RQ, Francischetti IMB (2013).Desmolaris, a novel Factor XIa anticoagulant from the salivary gland of the vampire bat (Desmodus rotundus) inhibits inflammation and thrombosis.Blood 122(25):4094-4106.     (I.F.: 16.6)

24. Schwarz A, von Reumont BM, Erhart J, Chagas AC, Ribeiro JMC#, Kotsyfakis M# (2013).De novo Ixodes ricinus salivary transcriptome analysis using two different next generation sequencing methodologies.The FASEB Journal 27(12):4745-4756.     (I.F.: 5.4)

23. Valdes JJ, Schwarz A, Cabeza de Vaca I, Calvo E, Pedra JHF, Guallar V#, Kotsyfakis M# (2013). Tryptogalinin is a tick Kunitz serine protease inhibitor with a unique intrinsic disorder.PLoS One 8(5):e62562.     (I.F.: 2.8)

22. Chen G, Severo MS, Sohail M, Sakhon OS, Wikel SK, Kotsyfakis M, Pedra JH (2012).Ixodes scapularis saliva mitigates inflammatory cytokine secretion during Anaplasma phagocytophilum stimulation of immune cells.Parasit Vectors 5(1):229.     (I.F.: 3)

21. Collin N*, Assumpção TCF*, Mizurini DM, Gilmore D, Dutra-Oliveira A, Kotsyfakis M, Sá-Nunes A, Teixeira C, Ribeiro JMC, Monteiro RQ, Valenzuela JG, Francischetti IMB (2012). Lufaxin, a novel Factor Xa inhibitor from the salivary gland of the sand fly Lutzomyia longipalpis, blocks PAR2 activation and inhibits inflammation and thrombosis in vivo. Arterioscler Thromb Vasc Biol. 32(9):2185-2198.     (I.F.: 6.6)

20. Chen G, Severo MS, Sakhon OS, Choy A, Herron MJ, Felsheim RF, Wiryawan H, Liao J, Johns JL, Munderloh UG, Sutterwala FS, Kotsyfakis M, Pedra JHF (2012).Anaplasma phagocytophilum LPDA1 affects host-derived immunopathology during microbial colonization.Infect Immun. 80(9):3194-3205.     (I.F.: 3.2)

19. Ronca R*, Kotsyfakis M*, Lombardo F*, Rizzo C, Curra C, Ponzi M, Fiorentino G, Ribeiro JMC and Arca B (2012). The Anopheles gambiae cE5, a tight- and fast-binding thrombin inhibitor with post-transcriptionally regulated salivary-restricted expression.Insect Biochem Mol Biol. 42(9):610-620.     (I.F.: 3.6)

18. Rodrigues J, Oliveira GA, Kotsyfakis M, Dixit R, Molina-Cruz A, Jochim R, Barillas-Mury C (2012). An epithelial serine protease, AgESP, is required for Plasmodium invasion in the mosquito Anopheles gambiae.PLoS One 7(4):e35210.     (I.F.: 2.8)

17. Tsujimoto H, Kotsyfakis M, Francischetti IM, Eum JH, Strand MR, Champagne DE (2012). Simukunin from the salivary glands of the black fly Simulium vittatum inhibits enzymes that regulate clotting and inflammatory responses.PLoS One 7(2):e29964.     (I.F.: 2.8)

16. Horka H*, Staudt V*, Klein M*, Taube C, Reuter S, Dehzad N, Andersen JF, Kopecky J, Schild H, Kotsyfakis M, Hoffmann M, Gerlitzki B, Stassen M, Bopp T, Schmitt E (2012). The tick salivary protein sialostatin L inhibits the Th9-derived production of the asthma-promoting cytokine IL-9 and is effective in the prevention of experimental asthma.J Immunol. 188(6):2669-2676.     (I.F.: 4.7)

15. Francischetti IM, Oliveira CJ, Ostera GR, Yager SB, Debierre-Grockiego F, Carregaro V, Jaramillo-Gutierrez G, Hume JC, Jiang L, Moretz SE, Lin CK, Ribeiro JM, Long CA, Vickers BK, Schwarz RT, Seydel KB, Iacobelli M, Ackerman HC, Srinivasan P, Gomes RB, Wang X, Monteiro RQ, Kotsyfakis M, Sá-Nunes A, Waisberg M (2012). Defibrotide interferes with several steps of the coagulation inflammation cycle and exhibits therapeutic potential to treat severe malaria.Arterioscler Thromb Vasc Biol. 32(3):786-798.     (I.F.: 6.6)

14. Calvo E, Mizurini DM, Sá-Nunes A, Ribeiro JMC, Andersen JF, Mans BJ, Monteiro RQ, Kotsyfakis M, Francischetti IMB (2011). Alboserpin, a Factor Xa inhibitor from the mosquito vector of Yellow Fever, binds heparin and membrane phospholipids and exhibits antithrombotic activity.J Biol Chem. 286(32):27998-28010.     (I.F.: 4.1)

13. Chmelar J, Oliveira CJ, Rezacova P, Francischetti IMB, Kovarova Z, Pejler G, Kopacek P, Ribeiro JMC, Mares M, Kopecky J and Kotsyfakis M† (2011).  A tick salivary protein targets cathepsin G and chymase and inhibits host inflammation and platelet aggregation.Blood 117(3):736-744. Cover of the issue.     (I.F.: 16.6)

12Kotsyfakis M, Horka H, Salat J, Andersen JF (2010). The crystal structures of two salivary cystatins from the tick Ixodes scapularis and the effect of these inhibitors on the establishment of Borrelia burgdorferi infection in a murine model.Mol Microbiol. 77(2):456-470.     (I.F.: 3.6)

11. Salat J, Paesen GC, Rezacova P, Kotsyfakis M, Kovarova Z, Sanda M, Majtan J, Grunclova L, Horka H, Andersen JF, Brynda J, Horn M, Nunn MA, Kopacek P, Kopecky J, Mares M (2010). Crystal structure and functional characterisation of an immunomodulatory salivary cystatin from the soft tick Ornithodoros moubata.Biochem J. 429(1):103-112.     (I.F.: 4.3)

10. Francischetti IMB, Kotsyfakis M, Andersen JF, Lukszo J (2010). Cyr61/CCN1 displays high-affinity binding to the Somatomedin B 1–44 domain of Vitronectin. PLoS One 5(2):e9356.     (I.F.: 2.8)

9. Calvo E, Sanchez-Vargas I, Kotsyfakis M, Favreau AJ, Barbian KD, Pham VM, Olson KE, Ribeiro JMC (2010). The salivary gland transcriptome of the eastern treehole mosquito, Ochlerotatus triseriatus.J Med Entomol. 47(3):376-386.     (I.F.: 1.9)

8.  Sá-Nunes A, Bafica A, Antonelli LRV, Choi EY, Francischetti IMB,  Andersen JF, Shi GP, Chavakis T, Ribeiro JMC, Kotsyfakis M† (2009). Sialostatin L inhibits antigen‑specific T cell proliferation and ameliorates experimental autoimmune encephalomyelitis.J Immunol. 182(12):7422-7429.     (I.F.: 4.7)Publication of research articles in international peer-reviewed journals prior to 2009 (as a postdoc and PhD student, † corresponding author)

7.  Francischetti IMB, Meng Z, Mans BJ, Gudderra N, Hall M, Veenstra TD, Pham VM, Kotsyfakis M, Ribeiro JMC (2008). An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus.J Proteomics. 71(5):493-512.     (I.F.: 3.5)

6.  Kotsyfakis M†, Anderson JM, Andersen JF, Calvo E, Francischetti IMB, Mather TN, Valenzuela JG, Ribeiro JMC (2008). Immunity against a "silent" salivary antigen of the Lyme vector Ixodes scapularis impairs its ability to feed.J Immunol. 181(8):5209-5212. Cutting Edge Article.     (I.F.: 4.7)

5Kotsyfakis M, Karim S, Andersen JF, Mather TN, Ribeiro JMC (2007). Selective cysteine protease inhibition contributes to blood feeding success of the tick Ixodes scapularis.J Biol Chem. 282(5):29256-29263.     (I.F.: 4.1)

4. Kotsyfakis M, Sá-Nunes A, Francischetti IMB, Mather TN, Andersen JF, Ribeiro JMC (2006). Antiinflammatory and immunosuppressive activity of sialostatin L, a salivary cystatin from the tick Ixodes scapularis.J Biol Chem. 281(36):26298-26307.     (I.F.: 4.1)

3.  Kotsyfakis M, Vontas J, Siden-Kiamos I, Louis C (2005). The annexin gene family in the malaria mosquito Anopheles gambiae. Insect Mol Biol. 14(5):555-562.     (I.F.: 2.4)

2. Kotsyfakis M, Ehret-Sabatier L, Siden-Kiamos I, Mendoza J, Sinden RE, Louis C (2005). Plasmodium berghei ookinetes bind to Anopheles gambiae and Drosophila melanogaster annexins. Mol Microbiol. 57(1):171-179.     (I.F.: 3.6)

1.  Spanos L, Koutroumbas G, Kotsyfakis M, Louis C (2000). The mitochondrial genome of the Mediterranean fruit fly, Ceratitis capitata.Insect Mol Biol. 9(4):139-144.     (I.F.: 2.4)

IIB2) Publication of review articles in international peer-reviewed journals 
(* equal contribution, † corresponding author)

14. Martins LA, Kotál J, Bensaoud C, Chmelař J, Kotsyfakis M† (2020). Small protease inhibitors in tick saliva and salivary glands and their role in tick-host-pathogen interactions. Biochim Biophys Acta Proteins Proteom. 1868(2):140336.     (I.F.: 2.5)

13. Bensaoud C, Hackenberg M, Kotsyfakis M† (2019). Noncoding RNAs in parasite-vector-host interactions. (Most read article in Trends in Parasitology)Trends Parasitol. 35(9):715-724.     (I.F.: 8)

12. Chmelař J, Kotál J, Kovaříková A, Kotsyfakis M† (2019). The use of tick salivary proteins as novel therapeutics. Front Physiol. 10:812.     (I.F.: 3.2)

11. Oliva Chavez AS, O’Neal AJ, Santambrogio L, Kotsyfakis M, Pedra JH (2019).Message in a vesicle: transkingdom communication at the vector-host Interface.J Cell Sci. 132(6). pii: jcs224212.     (I.F.: 4.5)

10. Hackenberg M, Kotsyfakis M† (2018). Exosome-mediated pathogen transmission by arthropod vectors. Trends Parasitol. 34(7):549-552.     (I.F.: 8)

9. Chmelař J, Kotál J, Langhansová H, Kotsyfakis M† (2017). Protease inhibitors in tick saliva: the role of serpins and cystatins in tick-host-pathogen Interaction.Front Cell Infect Microbiol. 7:216.     (I.F.: 3.5)

8. Chmelař J, Kotál J, Kopecky J, Pedra JH, Kotsyfakis M† (2016).All for one and one for all on the tick-host battlefield. Trends Parasitol. 32(5):368-377.     (I.F.: 8)

7. Shaw D, Kotsyfakis M, Pedra JH (2016).For whom the bell Tolls (and Nods): Spit-acular saliva.Curr Trop Med Rep. 3(2):40-50.     (I.F.: pending)

6. Chmelař J, Kotál J, Karim S, Kopacek P, Francischetti IMB, Pedra JH, Kotsyfakis M† (2016).Sialomes and mialomes: a systems biology view of tick tissues and tick-host interactions.Trends Parasitol. 32(3):242-254.     (I.F.: 8)

5. Kotál J, Langhansová H, Lieskovská J, Andersen JF, Francischetti IM, Chavakis T, Kopecký J, Pedra JH, Kotsyfakis M*†, Chmelař J* (2015). Modulation of host immunity by tick saliva. J Proteomics 128:58-68.     (I.F.: 3.5)

4. Sakhon OS, Severo MS, Kotsyfakis M, Pedra JHF (2013).A Nod to disease vectors: mitigation of pathogen sensing by arthropod saliva.Frontiers in Microbiology 4, Article 308:1-14.     (I.F.: 4.3)

3. Schwarz A, Valdes J, Kotsyfakis M† (2012). The role of cystatins in tick physiology and blood feeding. Ticks Tick Borne Dis. 3(3):117-127.     (I.F.: 3.1)

2. Severo MS, Stephens KD, Kotsyfakis M, Pedra JH (2012). Anaplasma phagocytophilum: deceptively simple or simply deceptive? Future Microbiol. 7(6):719-731.     (I.F.: 2.7)

1. Chmelar J, Calvo E, Pedra JH, Francischetti IM, Kotsyfakis M† (2012). Tick salivary secretion as a source of antihemostatics.J Proteomics 75(13):3842-3854.     (I.F.: 3.5)

IIB3) Book chapters
(† corresponding author)

3. Horka H, Chagas AC, Andersen JF, Kopecky J, Kotsyfakis M†(2012). Tick saliva-mediated immunomodulation of the vertebrate host. In Woldemeskel M. (Ed.) Ticks: Management, Disease Control and the Environment, Nova Science Publishers, Inc., 2012, p. 19-36. ISBN:978-1-62081-136-8

2. Sojka D, Francischetti IMB, Calvo E, Kotsyfakis M† (2011). Cysteine proteases from bloodfeeding arthropod ectoparasites. In: Robinson MW, Dalton JP (Eds.). Advances in Experimental Medicine and Biology 712: Cysteine proteases of pathogenic organisms. Landes Bioscience and Springer, 2011, p. 177-187. ISBN:978-1-4419-8413-5.

1. Chmelar J, Francischetti IMB, Kotsyfakis M† (2010). Salivary protease inhibitors with non anti-hemostatic functions.In: Kini RM, McLane MA, Clemetson K, Markland FS, Morita T (Eds.). Toxins and Hemostasis: From Bench to Bedside. Springer, 2010, p. 153-164. ISBN:978-90-481-9294-6.

IIB4) Miscellaneous publication of research articles in international peer-reviewed journals
(† corresponding author)

4. Kotsyfakis M, Patelarou E (2019).MicroRNAs as biomarkers of harmful environmental and occupational exposures: a systematic review. Biomarkers 24(7): 623-630.      (I.F.: 1.7)

3. Kotsyfakis M, Sarogiannis S, Patelarou E (2019).A systematic review of the health impact of Saharan dust exposure.International Journal of Occupational Medicine and Environmental Health 32(6):749-760.     (I.F.: 1.3)

2. Barturen G, Rueda A, Hamberg M, Alganza A, Lebron R, Kotsyfakis M, Shi BJ, Koppers-Lalic D, Hackenberg M (2014). sRNAbench: profiling of small RNAs and its sequence variants in single or multi-species high-throughput experiments. Methods in Next Generation Sequencing 1:21-31.     (I.F.: pending)

1. Anagnostopoulos A, Mouzakiti N, Ruthven S, Herod J, Kotsyfakis M† (2013). Primary cervical and uterine corpus lymphoma; a case report and literature review. Int J Clin Exp Med. 6(4):298-306.     (I.F.: 0.2)

IIB5) Patents
(† corresponding author)

3. Title:  Composition comprising mirnas for use as a drug
Patent Number:  WO2018197736A2, (PCT/ES2018/070325)
Patent Assignee: UNIVERSIDAD DE GRANADA, BIOLOGY CENTRE OF THE CZECH ACADEMY OF SCIENCES.
Inventors: Michail Kotsyfakis, Michael Hackenberg, Juan Antonio Marchal Corrales, Gonzalo Martinez Navaja, Houria Boulaiz Tassi.

2. Title: Inhibiting plasma coagulation and inhibiting elastase and/or cathepsin G in a subject, comprises administering an isolated simukunin polypeptide to the subject 
Patent Number:  WO2012162611-A1
Patent Assignee: UNIV GEORGIA RES FOUND INC; US DEPT HEALTH&HUMAN SERVICES
Inventors: Donald E. Champagne, Hitoshi Tsujimoto, Michail Kotsyfakis, Ivo Francischetti, Michael R. Strand.

1. Title:  Detection of tick infestation comprises administering cystatin antigens and monitoring for inflammatory responses or pain responses indicating tick infestation, where cystatin polypeptide comprises specified peptide sequences 
Patent Number:  WO2009017689-A2; WO2009017689-A3; US2010278752-A1
Patent Assignee: US DEPT HEALTH&HUMAN SERVICES; RHODE ISLAND HIGHER EDUCATION
Inventors: Michail Kotsyfakis†, Jennifer Anderson, John Andersen, Shahid Karim, Jesus Valenzuela, Thomas Mather, José Ribeiro. 

III. CITATION REPORT

As of July 24th, 2019 (source: Web of Science)
All citations: 1562 (1341 without self-citations). 
H-Index: 22 (source: Web of Science).

IV. REFEREES (alphabetically)

A) Triantafyllos Chavakis: Professor and Chief of the Division for Vascular Inflammation, Diabetes and Kidney, Department of Internal Medicine, University Clinic Carl Gustav Carus, Dresden University of Technology, Dresden, Germany.
Email: Triantafyllos.Chavakis@uniklinikum-dresden.de
Telephone: +49 (0)351 458 6007
B) Joao H Pedra: Associate Professor at the Department of Microbiology and ImmunologyUniversity of Maryland School of Medicine, Baltimore, USA.
Email: JPedra@som.umaryland.edu
Telephone: +1 (410)706-7343 (office)
C) JoseMC Ribeiro: Head of the Vector Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA.
E-mail: jribeiro@mail.nih.gov
Telephone: +1 (301)496-9389 (office)

V. CONTACT INFORMATION

Michail Kotsyfakis 
Biology Centre, Czech Academy of Sciences
Branisovska 31
37005 Budweis
Czech Republic

Email: kotsyfakis@paru.cas.cz
Work Phone: (00420)387775491 (office)
(00420)387775492 (laboratory)

Fax: (00420)385310388
Web pages: https://www.paru.cas.cz/Laboratory of genomics and proteomics of disease vectors

Home address: Branisovska 31
37005 Budweis
Czech Republic

Mobile phone: (00420)775656850
Alternate (personal) Email address: mich_kotsyfakis@yahoo.com

 

 

 

Teaching The Human Plasma Proteome
Faculty of Science, University of South Bohemia

Publications
Total found: 56 records
Kotál J., Bensaoud C., Chmelař J., Kotsyfakis M., Martins L. (2020) Small protease inhibitors in tick saliva and salivary glands and their role in tick-host-pathogen interactions BBA - Proteins and Proteomics 1868 : 140336.
DOI: 10.1016/j.bbapap.2019.140336
Bensaoud C., Hackenberg M., Kotsyfakis M. (2019) Noncoding RNAs in Parasite-Vector-Host Interactions Trends in Parasitology 35 : 715–724.
DOI: 10.1016/j.pt.2019.06.012
Chávez A.S.O., O´Neal A., Santambrogio L., Kotsyfakis M., Pedra J. (2019) Message in a Vesicle: Transkingdom Intercommunication at the Vector-Host Interface Journal of Cell Science 132 : jcs224212.
DOI: 10.1242/jcs.224212
Chmelař J., Kotál J., Kovaříková A., Kotsyfakis M. (2019) The Use of Tick Salivary Proteins as Novel Therapeutics Frontiers in Physiology 10 : 812.
DOI: 10.3389/fphys.2019.00812
Kotál J., Stergiou N., Buša M., Chlastáková A., Beránková Z., Řezáčová P., Langhansová H., Schwarz A., Calvo E., Kopecký J., Mareš M., Schmitt E., Chmelař J., Kotsyfakis M. (2019) The structure and function of Iristatin, a novel immunosuppressive tick salivary cystatin Cellular and Molecular Life Sciences 76 : 2003–2013.
DOI: 10.1007/s00018-019-03034-3
Kotsyfakis M., Patelarou E. (2019) MicroRNAs as biomarkers of harmful environmental and occupational exposures: a systematic review. Biomarkers 24 : 623-630.
DOI: 10.1080/1354750X.2019.1652348
Kotsyfakis M., Zarogiannis S.G., Patelarou E. (2019) A systematic review of the health impact of Saharan dust exposure International Journal of Occupational Medicine and Environmental Health 32 : 749–760.
DOI: 10.13075/ijomeh.1896.01466
Assumpção T., Mizurini D., Ma D., Monteiro R., Ahlstedt S., Reyes M., Kotsyfakis M., Mather T.N., Andersen J., Lukszo J., Ribeiro J., Francischetti I. (2018) Ixonnexin from tick saliva promotes fibrinolysis by interacting with plasminogen and tissue-type plasminogen activator, and prevents arterial thrombosis Scientific Reports 8 : 4806.
DOI: 10.1038/s41598-018-22780-1
Hackenberg M., Kotsyfakis M. (2018) Exosome-Mediated Pathogen Transmission by Arthropod Vectors Trends in Parasitology 34 : 549–552.
DOI: 10.1016/j.pt.2018.04.001
Chmelař J., Kotál J., Langhansová H., Kotsyfakis M. (2017) Protease inhibitors in tick saliva: the role of serpins and cystatins in tick-host-pathogen interaction Frontiers in Cellular and Infection Microbiology 7 : 216.
DOI: 10.3389/fcimb.2017.00216
Hackenberg M., Langenberger D., Schwarz A., Erhart J., Kotsyfakis M. (2017) In silico target network analysis of de novo-discovered, tick saliva-specific microRNAs reveals important combinatorial effects in their interference with vertebrate host physiology RNA 23 : 1259-1269.
DOI: 10.1261/rna.061168
Shaw D., McClure E.E., Kotsyfakis M., Pedra J. (2017) Tick saliva and microbial effector molecules: two sides of the same coin Arthropod Vector: Controller of Disease Transmission (Volume 2): Vector Saliva-Host-Pathogen Interactions, S.K. Wikel, S. Aksoy and G. Dimopoulos (Eds.) 169-194.
DOI: 10.1016/B978-0-12-805360-7.00010-1
Assumpção T., Ma D., Mizurini D., Kini R., Ribeiro J., Kotsyfakis M., Monteiro R., Francischetti I. (2016) In Vitro Mode of Action and Anti-thrombotic Activity of Boophilin, a Multifunctional Kunitz Protease Inhibitor from the Midgut of a Tick Vector of Babesiosis, Rhipicephalus microplus PLoS Neglected Tropical Diseases 10 : e0004298.
DOI: 10.1371/journal.pntd.0004298
Chmelař J., Kotál J., Karim S., Kopáček P., Francischetti I., Pedra J., Kotsyfakis M. (2016) Sialomes and Mialomes: A Systems-Biology View of Tick Tissues and Tick-Host Interactions Trends in Parasitology 32 : 242-254.
DOI: 10.1016/j.pt.2015.10.002
Chmelař J., Kotál J., Kopecký J., Pedra J., Kotsyfakis M. (2016) All for one and one for all on the tick–host battlefield Trends in Parasitology 32 : 368-377.
DOI: 10.1016/j.pt.2016.01.004
Ferreira V., Vale V., Pangburn M., Abdeladhim M., Mendes-Sousa A., Coutinho-Abreu I., Rasouli M., Brandt E., Meneses C., Lima III K., Araújo R., Pereira M., Kotsyfakis M., Oliveira F., Kamhawi S., Ribeiro J., Gontijo N., Collin N., Valenzuela J.G. (2016) SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis Scientific Reports 6 : 19300.
DOI: 10.1038/srep19300
Shaw D., Kotsyfakis M., Pedra J. (2016) For Whom the Bell Tolls (and Nods): Spit-acular Saliva Current Tropical Medicine Reports 3 : 40-50.
DOI: 10.1007/s40475-016-0072-4
Tonk M., Bouchut A., Pierrot C., Pierce R., Kotsyfakis M., Rahnamaeian M., Vilcinskas A., Khalife J., Valdés J. J. (2016) Antiplasmodial activity is an ancient and conserved feature of tick defensins Frontiers in Microbiology 7 : 1682.
DOI: 10.3389/fmicb.2016.01682
Wang X., Shaw D., Hammond H., Sutterwala F., Rayamajhi M., Shirey K., Perkins D., Bonventre J., Velayutham T., Evans S., Rodino K., VieBrock L., Scanlon K., Carbonetti N., Carlyon J., Miao E., McBride J., Kotsyfakis M., Pedra J. (2016) The prostaglandin E2-EP3 receptor axis regulates Anaplasma phagocytophilum-mediated NLRC4 inflammasome activation PLoS Pathogens 12 : e1005803.
DOI: 10.1371/journal.ppat.1005803
Wang X., Shaw D., Sakhon O., Snyder G., Sundberg E., Santambrogio L., Sutterwala F., Dumler J., Shirey K., Perkins D., Richard K., Chagas A., Calvo E., Kopecký J., Kotsyfakis M., Pedra J. (2016) The tick protein sialostatin L2 binds to annexin A2 and inhibits NLRC4-mediated inflammasome activation Infection and Immunity 84 : 1796-1805.
DOI: 10.1128/IAI.01526-15

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Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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