Laboratoř genomiky a proteomiky vektorů

In our group, we exploit the Ixodes ricinus tick as a model organism to:

  • Apply a systems-based and multimodal approach to discover tick effectors determining blood feeding success.
  • Understand which vertebrate host proteolytic cascades tick salivary secretions regulate at sites of tick infestation and how they facilitate blood meal uptake and successful completion of the tick lifecycle.
  • Test the pharmacological action(s) of recombinant tick salivary proteins and non-coding RNAs (long non coding and microRNAs) and their mechanism of action in the vertebrate host at the molecular, cellular, and organismal level to expedite drug development.

 
Our research projects aim to:

  • Explore basic mechanisms of tick blood feeding success as the conceptual basis for the future development of novel methods/tools (e.g., anti-tick vaccines and diagnostic kits to monitor exposure of vertebrate hosts to arthropod disease vectors) to control tick populations.
  • Demonstrate the pharmacological action of tick salivary proteins and non-coding RNAs in the regulation of vertebrate host homeostasis (haemostasis, vascular biology, immunity, neuromodulation) and/or as anti-microbial peptides and test their potential for drug development in the near future.

Research topics:

  • Gene overexpression/protein purification
  • Biochemistry: enzyme inhibition kinetics-protease inhibitors
  • Structural Biology
  • Proteomics/Transcriptomics/Non-coding RNAs
  • Immune assays: cell-culture assays /animal models
  • Tick colony maintenance/tick saliva production
Vybrané publikace:
Bensaoud C., Hackenberg M., Kotsyfakis M. (2019) Noncoding RNAs in Parasite-Vector-Host Interactions Trends in Parasitology 35: 715–724.
DOI: 10.1016/j.pt.2019.06.012
Kotál J., Stergiou N., Buša M., Chlastáková A., Beránková Z., Řezáčová P., Langhansová H., Schwarz A., Calvo E., Kopecký J., Mareš M., Schmitt E., Chmelař J., Kotsyfakis M. (2019) The structure and function of Iristatin, a novel immunosuppressive tick salivary cystatin Cellular and Molecular Life Sciences 76: 2003–2013.
DOI: 10.1007/s00018-019-03034-3
Hackenberg M., Kotsyfakis M. (2018) Exosome-Mediated Pathogen Transmission by Arthropod Vectors Trends in Parasitology 34: 549–552.
DOI: 10.1016/j.pt.2018.04.001
Schwarz A., Von Reumont B., Erhart J., Chagas A., Ribeiro J., Kotsyfakis M. (2013) De novo Ixodes ricinus salivary gland transcriptome analysis using two next-generation sequencing methodologies FASEB JOURNAL 27: 4745-4756.
DOI: 10.1096/fj.13-232140
Chmelař J., Oliveira C., Řezáčová P., Francischetti I., Kovářová Z., Pejler G., Kopáček P., Ribeiro J., Mareš M., Kopecký J., Kotsyfakis M. (2011) A tick salivary protein targets cathepsin G and chymase and inhibits host inflammation and platelet aggregation. Blood 117: 736–744.
DOI: 10.1182/blood-2010-06-293241

Všechny publikace (76)

KONTAKT

Biologické centrum AV ČR, v.v.i.
Parazitologický ústav
Branišovská 1160/31
370 05 České Budějovice

NAJÍT PRACOVNÍKA