Michalis Kotsyfakis, PhD.
Vedoucí , Laboratoř genomiky a proteomiky vektorů
Výuka
The Human Plasma Proteome
Přírodovědecká fakulta Jihočeské univerzity
Současné publikace
Anagnostopoulos A., Mouzakiti N., Ruthven S., Herod J., Kotsyfakis M. (2013) Primary cervical and uterine corpus lymphoma; a case report and literature review International journal of clinical and experimental medicine 6: 298-306.
Valdés J.J, Schwarz A., Cabeza de Vaca I., Calvo E., Pedra J.H.F., Guallar V., Kotsyfakis M. (2013) Tryptogalinin Is a Tick Kunitz Serine Protease Inhibitor with a Unique Intrinsic Disorder PloS ONE 8: e62562.
Chen G, Severo M.S., Sohail M., Sakhon O.S., Wikel S.K., Kotsyfakis M., Pedra J.H. (2012) Ixodes scapularis saliva mitigates inflammatory cytokine secretion during Anaplasma phagocytophilum stimulation of immune cells. Parasites & Vectors 5: 229. (link)
Collin N., Assumpção T.C.F., Mizurini D.M., Gilmore D., Dutra-Oliveira A., Kotsyfakis M., Sa-Nunes A., Teixeira C., Ribeiro J.M.C., Monteiro R.Q., Valenzuela J.G., Francischetti I.M.B. (2012) Lufaxin, a novel factor Xa inhibitor from the salivary gland of the sand fly Lutzomyia longipalpis, blocks protease-activated receptor 2 activation and inhibits inflammation and thrombosis in vivo. Arteriosclerosis Thrombosis and Vascular Biology 32: 2185–2198.
Ronca R., Kotsyfakis M., Lombardo F., Rizzo C., Currà C., Ponzi M., Fiorentino G., Ribeiro J.M.C., Arcà B. (2012) The Anopheles gambiae cE5, a tight- and fast-binding thrombin inhibitor with post-transcriptionally regulated salivary-restricted expression. Insect Biochemistry and Molecular Biology 42: 610–620.
Odborný životopis
Employment history
09/2009 - till now:Headof the Laboratory of Genomics and Proteomics of Disease Vectors, Institute of Parasitology, Biology Centre, Academy of Sciences ofCzechRepublic, Czech Republic.
3/2005 - 8/2009:Post-doctoral research associate at the Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA.
Mentor: Professor J. M. C. Ribeiro
12/2004 - 2/2005:Post-doctoral fellow at the Laboratory of Insect Molecular Genetics, Institute of Molecular Biology and Biotechnology, FORTH, Greece.
Mentor: Professor C. Louis
Academic record
10/2000 - 11/2004:Ph. D. degree in Molecular Biology and Biomedicine, Joint Graduate Program of the Departments of Medicine and Biology, University of Crete, and the Institute of Molecular Biology and Biotechnology, FORTH, Greece.
Thesis title: A study of the Anopheles annexin family as proteins that bind Plasmodiumberghei parasites (2004)
Advisor: Professor C. Louis
10/1998 - 9/2000:M. Sc. degree in Molecular Biology and Biomedicine, Joint Graduate Program of the Departments of Medicine and Biology, University of Crete, and the Institute of Molecular Biology and Biotechnology, FORTH, Greece.
Thesis title: Studies on the interaction of Plasmodium parasites with Anopheles mosquitoes (2000)
Advisor: Professor C. Louis
9/1994 - 9/1998:B.Sc. degree in Biology, University of Crete, Greece.
Selected oral presentations/ talks in international conferences (the last 4 years)
- Invited seminar at the University of Southern Mississippi, Department of Biological Sciences, 5 October 2012, Hattiesburg, Mississipi, USA.
- Invited symposium speaker, 24th International Congress of Entomology, 19-24 August 2012, Daegu, South Korea.
-Invited seminar at the University of California-Riverside, Department of Entomology, Center for Disease Vector Research, 12 May 2012, Riverside California, USA.
- Seventh International Conference on Ticks and Tick-borne Pathogens, 28 August-2 September 2011, Zaragoza, Spain (co-chair of a physiology, biochemistry and molecular biology session).
- Fifth International Meeting on Molecular and Population Biology of Mosquitoes and Other Disease Vectors, 24-30 July 2011, Kolymbari, Crete, Greece (co-chair of a physiology session).
- German Society for Parasitology Annual Meeting, 16-20 March 2010, Düsseldorf, Germany (co-chair of a biochemical session).
- Research Seminars in a) the Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, USA
and b) Technische Universität Dresden, Dresden, Germany, November 2009 (Invited speaker).
- Research seminars in a) the Institute of Parasitology, National Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
and b) the Department of Molecular Biology and Genetics, Alexandroupolis, Greece
and c) the Institute of Biology, National Center for Scientific Research “DEMOKRITOS”, Athens, Greece, March 2009 (Invited speaker).
- VI International Conference on Ticks and Tick-borne Pathogens, 21-26 September 2008, Buenos Aires, Argentina.
Fellowships
- 2/2010 - 2/2012:Alexander von Humboldt fellowship for Dr. Alexandra Schwarz to join my group as a post doctoral fellow (host laboratory), 26,000 Euros per year - 52,000 Euros total.
- 9/2009 - 8/2014:Jan Evangelista Purkyně Fellowship from the Academy of Sciences of the Czech Republic (the first to receive the fellowship in South Bohemia and the first non-Czech speaker), 30,000 Euros per year - 150,000 Euros total.
- 3/2005 - 10/2009:Post-doctoral Visiting Fellowship, National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA (Intramural grant number 0012117069).
- 6/2002 - 8/2002:EMBO Short Term Fellowship (STF number 67-2002).
Memberships and awards
- 1/2008:American Society for Biochemistry and Molecular Biology Postdoctoral Travel Award to participate in 2008 meeting of the society in San Diego, USA.
- 11/2007 - till now:American Society for Biochemistry and Molecular Biology Member.
- 9/2007:NIH Fellows Award for Research Excellence in Biomedical Research.
- 8/2007:International Proteolysis Society travel award to participate in the 5th IPS meeting in Patras, Greece.
- 1/2007:Young Scientist Award to participate in the 10th International Union of Biochemistry and Molecular Biology/Sociedade Brasileira de Bioquimica e Biologia (IUBMB/SBBq) meeting in Salvador, Brazil, May 21-25, 2007.
- 9/2006:NIH Fellows Award for Research Excellence in Biomedical Research.
-10/2005 - 10/2009:International Proteolysis Society Member.
- 8/2005:International Proteolysis Society travel award to participate in the 4th IPS meeting in Quebec, Canada.
Scientific reviewers
- Associate editor in the American Journal of Blood Research.
- External grant reviewer for France’s Agence Nationale de la Recherche (ANR).
- External reviewer for the journals: PNAS, PLoS One, PLoS Neglected Tropical Diseases, Journal of Proteomics, The FEBS Journal, BMC Genomics, Insect Biochemistry and Molecular Biology, Parasites and Vectors, Gene, Sensors.
Teaching experience
- 10/2011 – to date:Supervisor of a MSc. student (Jan Kotal) at the Molecular Biology Department, Faculty of Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic.
- Since January 2011:Co-supervisor of a PhD student (Veronika Dornakova), Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic.
- 10/2009 - 6/2011:Supervisor of two undergraduate students (Barbora Singerova and Jan Kotal) and one MSc. student (Lubos Kapusta) at the Faculty of Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic.
- 10/2009 - 5/2011:Co-supervisor in the PhD thesis of Dr. Helena Horka, Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic.
- 2/2008 - 11/2010:Co-supervisor in the PhD thesis of Dr. Jindrich Chmelar, Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic.
- 3/2003 - 6/2003:Assistant in the supervision of two graduate students during their practical exercise at the Insect Molecular Genetics Lab., Institute of Molecular Biology and Biotechnology, FORTH, Greece.
- 9/2000 - 1/2001:Teaching Assistant during the practical exercise in Microbiology laboratories of first year undergraduate students, Department of Biology, University of Crete, Greece.
- 9/1999 - 1/2000:Teaching Assistant during the practical exercise in Immunobiology laboratories of third year undergraduate students, Department of Biology, University of Crete, Greece.
Publication of research papers in international peer-reviewed journals
21.Collin N*, Assumpção TCF*, Mizurini DM, Gilmore D, Dutra-Oliveira A, Kotsyfakis M, Sá-Nunes A, Teixeira C, Ribeiro JMC, Monteiro RQ, Valenzuela JG, Francischetti IMB.
Lufaxin, a novel Factor Xa inhibitor from the salivary gland of the sand fly Lutzomyia longipalpis, blocks PAR2 activation and inhibits inflammation and thrombosis in vivo.
Arterioscler Thromb Vasc Biol. Accepted manuscript in press, available online 12 July 2012.
PMID: 22796577.
(I.F. : 6.4)
20.Chen G, Severo MS, Sakhon OS, Choy A, Herron MJ, Felsheim RF, Wiryawan H, Liao J, Johns JL, Munderloh UG, Sutterwala FS, Kotsyfakis M, Pedra JHF.
Anaplasma phagocytophilum LPDA1 affects host-derived immunopathology during microbial colonization.
Infect Immun.Accepted manuscript in press, available online2 July 2012.
PMID: 22753375.
(I.F. : 4.2)
19. Ronca R*, Kotsyfakis M*, Lombardo F*, Rizzo C, Curra C, Ponzi M, Fiorentino G, Ribeiro JMC and Arca B (2012).
The Anopheles gambiaecE5, a tight- and fast-binding thrombin inhibitor with post-transcriptionally regulated salivary-restricted expression.
Insect Biochem Mol Biol.Accepted manuscript in press, available online 14 May 2012. PMID:22647725.
(I.F. : 3.2)
18. Rodrigues J, Oliveira GA, Kotsyfakis M, Dixit R, Molina-Cruz A, Jochim R, Barillas-Mury C (2012).
An epithelial serine protease, AgESP, is required for Plasmodium invasion in the mosquito Anopheles gambiae.
PLoS One.7(4):e35210.
(I.F. : 4.1)
17.Tsujimoto H, Kotsyfakis M, Francischetti IM, Eum JH, Strand MR, Champagne DE (2012).
Simukunin from the salivary glands of the black fly Simulium vittatum inhibits enzymes that regulate clotting and inflammatory responses.
PLoS One.7(2):e29964.
(I.F. : 4.1)
16.Horka H*, Staudt V*, Klein M*, Taube C, Reuter S, Dehzad N, Andersen JF, Kopecky J, Schild H, Kotsyfakis M, Hoffmann M, Gerlitzki B, Stassen M, Bopp T, Schmitt E (2012).
The tick salivary protein sialostatin L inhibits the Th9-derived production of the asthma-promoting cytokine IL-9 and is effective in the prevention of experimental asthma.
J Immunol.188(6):2669-2676.
(I.F. : 5.8)
15.Francischetti IM, Oliveira CJ, Ostera GR, Yager SB, Debierre-Grockiego F, Carregaro V, Jaramillo-Gutierrez G, Hume JC, Jiang L, Moretz SE, Lin CK, Ribeiro JM, Long CA, Vickers BK, Schwarz RT, Seydel KB, Iacobelli M, Ackerman HC, Srinivasan P, Gomes RB, Wang X, Monteiro RQ, Kotsyfakis M, Sá-Nunes A, Waisberg M (2012).
Defibrotide interferes with several steps of the coagulation inflammation cycle and exhibits therapeutic potential to treat severe malaria.
Arterioscler Thromb Vasc Biol. 32(3):786-798.
(I.F. : 6.4)
14. Calvo E, Mizurini DM, Sá-Nunes A, Ribeiro JMC, Andersen JF, Mans BJ, Monteiro RQ, Kotsyfakis M, Francischetti IMB (2011).
Alboserpin, a Factor Xa inhibitor from the mosquito vector of Yellow Fever, binds heparin and membrane phospholipids and exhibits antithrombotic activity.
J Biol Chem.286(32):27998-28010.
(I.F. : 4.8)
13. Chmelar J, Oliveira CJ, Rezacova P, Francischetti IMB,Kovarova Z, Pejler G, Kopacek P, Ribeiro JMC, Mares M,Kopecky J and Kotsyfakis M† (2011).
A tick salivary protein targets cathepsin G and chymase and inhibits host inflammation and platelet aggregation.
Blood.117(3):736-744. Cover of the issue.
(I.F. : 9.9)
12. Kotsyfakis M, Horka H, Salat J, Andersen JF (2010).
The crystal structures of two salivary cystatins from the tick Ixodes scapularis and the effect of these inhibitors on the establishment of Borrelia burgdorferi infection in a murine model.
Mol Microbiol.77(2):456-470.
(I.F. : 5)
11. Salat J, Paesen GC, Rezacova P, Kotsyfakis M, Kovarova Z, Sanda M, Majtan J, Grunclova L, Horka H, Andersen JF, Brynda J, Horn M, Nunn MA, Kopacek P, Kopecky J, Mares M (2010).
Crystal structure and functional characterisation of an immunomodulatory salivary cystatin from the soft tick Ornithodoros moubata.
Biochem J.429(1):103-112.
(I.F. : 4.9)
10. Francischetti IMB, Kotsyfakis M, Andersen JF, Lukszo J (2010).
Cyr61/CCN1 Displays High-Affinity Binding to the Somatomedin B 1–44 Domain of Vitronectin.
PLoS One.5(2):e9356.
(I.F. : 4.1)
9. Calvo E, Sanchez-Vargas I, Kotsyfakis M, Favreau AJ, Barbian KD, Pham VM, Olson KE, Ribeiro JMC (2010).
The salivary gland transcriptome of the eastern tree hole mosquito,Ochlerotatus triseriatus.
J Med Entomol.47(3):376-386.
(I.F. : 1.8)
8. Sá-Nunes A, Bafica A, Antonelli LRV, Choi EY, Francischetti IMB, Andersen JF, Shi GP, Chavakis T, Ribeiro JMC, Kotsyfakis M†(2009).
Sialostatin L inhibits antigen‑specific T cell proliferation and ameliorates experimental autoimmune encephalomyelitis.
J Immunol.182(12):7422-7429.
(I.F. : 5.8)
7. Francischetti IMB, Meng Z, Mans BJ, Gudderra N, Hall M, Veenstra TD, Pham VM, Kotsyfakis M, Ribeiro JMC (2008).
An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus.
J Proteomics.71(5):493-512.
(I.F. : 4.9)
6. Kotsyfakis M†, Anderson JM, Andersen JF, Calvo E, Francischetti IMB, Mather TN, Valenzuela JG, Ribeiro JMC (2008).
Immunity against a "silent" salivary antigen of the Lyme vector Ixodes scapularis impairs its ability to feed.
J Immunol.181(8):5209-5212. Cutting Edge Article.
(I.F. : 5.8)
5. Kotsyfakis M, Karim S, Andersen JF, Mather TN, Ribeiro JMC (2007).
Selective cysteine protease inhibition contributes to blood feeding success of the tick Ixodes scapularis.
J Biol Chem. 282(5):29256-29263.
(I.F. : 4.8)
4.Kotsyfakis M, Sá-Nunes A, Francischetti IMB, Mather TN, Andersen JF, Ribeiro JMC (2006).
Antiinflammatory and immunosuppressive activity of sialostatin L, a salivary cystatin from the tick Ixodes scapularis.
J Biol Chem.281(36):26298-26307.
(I.F. : 4.8)
3. Kotsyfakis M,Vontas J, Siden-Kiamos I, Louis C (2005).
The annexin gene family in the malaria mosquito Anopheles gambiae.
Insect Mol Biol.14(5):555-562.
(I.F. : 2.5)
2.Kotsyfakis M, Ehret-Sabatier L, Siden-Kiamos I, Mendoza J, Sinden RE, Louis C (2005).
Plasmodium bergheiookinetes bind to Anopheles gambiae and Drosophila melanogaster annexins.
Mol Microbiol.57(1):171-179.
(I.F. : 5)
1. Spanos L, Koutroumbas G, Kotsyfakis M, Louis C (2000).
The mitochondrial genome of the Mediterranean fruit fly, Ceratitis capitata.
Insect Mol Biol. 9(4):139-144.
(I.F. : 2.5)
† corresponding author, * equal contribution
Total Impact Factor of research publications: 100.8
Average Impact Factor per research publication: 4.8
Publication of review papers in international peer-reviewed journals
3. Schwarz A, Valdes J, Kotsyfakis M†(2012).
The role of cystatins in tick physiology and blood feeding.
Ticks Tick Borne Dis.3(3):117-127.
(I.F. : 2.4)
2. Severo MS, Stephens KD, Kotsyfakis M, Pedra JH (2012).
Anaplasma phagocytophilum: deceptively simple or simply deceptive?
Future Microbiol.7(6):719-731.
(I.F. : 3.8)
1. Chmelar J, Calvo E, Pedra JH, Francischetti IM, Kotsyfakis M†(2012).
Tick salivary secretion as a source of antihemostatics.
J Proteomics.75(13):3842-3854.
(I.F. : 4.9)
† corresponding author
Total Impact Factor of review publications: 11.1
Average Impact Factor per review publication: 3.3
Book chapters
3. Horka H, Chagas AC, Andersen JF, Kopecky J, Kotsyfakis M†(2012).
Tick saliva-mediated immunomodulation of the vertebrate host.
In Woldemeskel M. (Ed.) Ticks: Management, Disease Control and the Environment, Nova Science Publishers, Inc., 2012, p. 19-36. ISBN:978-1-62081-136-8.
2. Sojka D, Francischetti IMB, Calvo E, Kotsyfakis M† (2011).
Cysteine proteases from bloodfeeding arthropod ectoparasites.
In: Robinson MW, Dalton JP (Eds.). Advances in Experimental Medicine and Biology 712: Cysteine proteases of pathogenic organisms. Landes Bioscience and Springer, 2011, p. 177-187.
ISBN:978-1-4419-8413-5.
1. Chmelar J, Francischetti IMB, Kotsyfakis M† (2010).
Salivary protease inhibitors with non anti-hemostatic functions.
In: Kini RM, McLane MA, Clemetson K, Markland FS, Morita T (Eds.). Toxins and Hemostasis: From Bench to Bedside. Springer, 2010, p. 153-164. ISBN:978-90-481-9294-6.
† corresponding author
Patents
2. Donald E. Champagne, Hitoshi Tsujimoto, Michail Kotsyfakis, Ivo Francischetti, Michael R. Strand.
Title: Simukunin
US Provisional Patent Application Serial No. 61/490,149.
1.Michail Kotsyfakis†, Jennifer Anderson, John Andersen, Shahid Karim, Jesus Valenzuela, Thomas Mather, José Ribeiro.
Title: Methods for Detection and Prevention of Tick Infestation and Pathogen Transmission.
US Patent Application 200809075.
† corresponding author
External Funding
1. Marie Curie EU-Reintegration grant, Grant agreement PIRG07-GA-2010-268177.
Role: PI
Project duration: 01 September 2010- 31 August 2014
Budget: 25,000 Euros annually - 100,000 Euros total.
Title: Exploring the salivary transcriptome of Ixodes ricinus, the Lyme disease vector in Europe, and the potential role of its cystatins in pathogen transmission.
2. Bilateral collaborative grant between German and Czech Research Institutions, Grant Agency of Czech Republic, Grant number: P302/11/J029.
Role: co-PI, (10%).
Project duration: 01 January 2011- 31 December 2013.
Total budget: 40,000 Euros annually- 120,000 Euros total.
Title: Effect of tick saliva cystatins on Th9 cells and the development of experimental asthma.
3. National Institutes of Health, USA, R01 grant, Grant Number 1R01AI093653-01A1
Role: co-PI
Project duration: 01 July 2011- 31 May 2016.
Budget: 25000 USD annually for my lab - 125,000 USD total.
Title: Rickettsial Immunity During Tick Transmission.
4.Grant Agency of Czech Republic, Grant number: P502/12/2409.
Role: PI.
Project duration: 01 January 2012 - 31 December 2015.
Total budget: 90000 Euros annually - 360,000 Euros total.
Title: Investigating the role of tick salivary protease inhibitors at the interface between ticks, pathogens and the vertebrate host.
Ongoing collaborations (alphabetically)
Bruno Arca, Professor at the Department of Structural and Functional Biology, University Federico II, Naples, Italy and Dipartimento di Scienze di Sanità Pubblica, Sezione di Parassitologia, Università “La Sapienza”, Italy.
Triantafyllos Chavakis,Professor and Chief of the Division for Vascular Inflammation, Diabetes and Kidney, Department of Internal Medicine, University Clinic Carl Gustav Carus, Dresden University of Technology, Germany.
Joao H Pedra, Assistant Professor at the University of California Riverside, USA.
José MC Ribeiro, Head of the Vector Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA.
Jesus G Valenzuela, Head of the Vector Molecular Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA.
Pro porovnávací studie jsou helmintologům z různých zemí k dispozici rozsáhlé sbírky...
