Date: 10.06.2019

Symposium “Volume Microscopy of Sensitive Samples: Advantages and Drawbacks”

10–11 June 2019 - The shared campus of Biology Centre and University of South Bohemia, Branisovska Street 31, Ceske Budejovice

Preliminary program:  
 
Monday, 10th June 2019
  8:30–9:30 Registration
  9:30          Welcome: Libor Grubhoffer (Biology Centre, České Budějovice)
  9:40          Keynote 1: Yannick Schwab (EMBL Heidelberg): Targeting strategies for FIBSEM imaging in multicellular specimens - from brain metastasis to plankton symbionts.
10:10          Keynote 2: Christel Genoud (FMI for Biomedical Research, Basel): Optimization of SBEM for acquisition of large volume: challenges from sample preparation to segmentation.
10:40          Lecture: Réza Shahidi (University of Exeter): Resolving connectomic studies by ssSEM
11:00          Lecture: Marie Vancova (Biology Centre, České Budějovice): Applications of volume electron microscopy in research on ticks and tick-borne pathogens

11:20          Coffe break
11:40          Keynote 3: Irina Kolotueva (University of Lausanne): TBA
12:10      Joint lecture: Gareth Griffiths (University of Oslo): Visualising nanoparticles for drug therapy in the zebrafish embryos: by fluorescence microscopy. Jens Wohlmann (University of Oslo): Visualising nanoparticles for drug therapy in the zebrafish embryos: by EM.
12:50         Lecture: Eva Ďurinová (Biology Centre, České Budějovice): New approaches to SBF SEM specimen preparation.

13:05          Lunch
13:50          Keynote 4: Miroslav Šlouf (Institute of Macromolecular Chemistry, Prague): Synthesis and characterization of novel embedding resins for 3D-SEM microscopy applications
14:20          Lecture: Markéta Dalecká (Biocev, Prague): Bubble-assisted workflow for 3D targeting of cells in correlative confocal and FIB-SEM microscopy
14:40          Company Lecture: Helmut Gnägi (Diatome): Serial sectioning for 3D reconstruction
15:00          Company lecture: Emine Korkmaz (Thermo Fischer Scientific): 3D large volume imaging- a powerful approach for cell and tissue studies
15:20          Company lecture: Jan Weert (Leica Microsystems): Artos 3D simplify your sample preparation workflow for Array tomography.

15:40        ​  Coffe break
16:00          Demonstration of Apreo Volume Scope (Thermo Fisher Scientific)/Artos system (Leica Microsystems)/ ATUMtome (RMC Boeckeler/ LEM tour

18:00          End
19:00        ​  Social evening with dinner
 
Tuesday, 11th June 2019:
  9:00        Demonstration of Apreo Volume Scope (Thermo Fisher Scientific)/Artos system (Leica Microsystems)/ ATUMtome (RMC Boeckeler/ LEM tour
11:00        Round table discussion – Specimen preparation for 3D SEM
12:00        End of symposium
 
Registration: through e-mail to Martina Tesarova (holland@paru.cas.cz)
Questions: Martina Tesarova (holland@paru.cas.cz)/ Jana Nebesarova (nebe@paru.cas.cz)
 
The symposium is organized with the support of the project “The Strategy AV21 Czech Academy of Sciences”, the research program “Diagnostic methods and techniques”
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CONTACT

Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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