Date: 19.06.2023

Biological Specimens in Electron Microscopes

19.–23. 6. 2023 České Budějovice

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Description: This practical course is focused on the advanced methods of biological specimen preparation for electron microscopy. The main goal is to make participants familiar with the theoretical background and the latest practical developments in this field. The programme includes state of the art fixation, including cryo-methods, cutting of ultrathin sections from embedded or frozen samples, methods of negative staining and immunolabeling, specimen preparation for transmission and scanning electron microscopy and methods of specimen preparation for 3D electron microscopy (single particle analysis, electron tomography, array tomography and serial block-face). The results will be evaluated using electron microscopes. The course is limited to 15 participants. It is intended for Master and PhD students, young researchers, technicians, and new EM users. This course is supported by the program for large research infrastructures of the Ministry of Education, Youth and Sports within the project “National Infrastructure for Biological and Medical Imaging (Czech-BioImaging)“.

OrganisersMarie Vancová and Jana Nebesářová (Laboratory of EM, Biology Centre CAS, České Budějovice)

Invited speakers:
Vladislav Krzyžánek
 (Institute of Scientific Instruments CAS, Brno)
Jiří Týč (Laboratory of EM, Biology Centre CAS, České Budějovice)
Zdeno Gardian (Laboratory of EM, Biology Centre CAS, České Budějovice)
Tomáš Bílý  (Macrocomplex group, South Bohemia University & Biology Centre of the CAS,České Budějovice)

Mgr. František Kitzberger, Mgr. Martina Tesařová, Mgr. Eva Ďurinová, Petra Masařová, Jiří Vaněček, Jana Kopecká


13:00 Welcome Address - Marie Vancová
13:10 Principles of transmission electron microscopy - Tomáš Bílý
14:00 Overview of TEM sample preparation methods - Jana Nebesářová
14:45 Coffee break
15:05 How to visualise macromolecules in TEM - Zdeno Gardian
16:00 Room temperature sectioning - Jana Nebesářová
8:30 Cryo methods in sample preparation - Marie Vancová
9:15 Principles of scanning electron microscopy - Vladislav Krzyžánek
10:00 Coffee break
10:20 Immunogold labeling - Marie Vancová
11:15 Specimen preparation for SEM - Jana Nebesářová
12:00 Lunch
13:00 Practicals
19:00 Course dinner
8:30 Electron tomography - Tomáš Bílý
9:30 Serial block-face SEM, array tomography - Jiří Týč
10:20 Coffee break
10:40 Introduction to image processing - Zdeno Gardian
11:25 Cellular ultrastructure and interpretation of images - Marie Vancová
12:10 Lunch
13:00 Practicals
8:30 Practicals
12:00 Lunch
13:00 Practicals
8:30 Practicals
12:00 Lunch
13:00 Round table discussion

Registration Fees
CSMS member: 6000 CZK
CSMS non-member: 7000 CZK
Industry: 10000 CZK
The course fee includes admission, the course materials, meals and coffee breaks
For registration click here
Contact: Martina Tesařová (

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Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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