Biological Specimens in Electron Microscopes
For more information click here: https://www.bc.cas.cz/
Description: This practical course is focused on the advanced methods of biological specimen preparation for electron microscopy. The main goal is to make participants familiar with the theoretical background and the latest practical developments in this field. The programme includes state of the art fixation, including cryo-methods, cutting of ultrathin sections from embedded or frozen samples, methods of negative staining and immunolabeling, specimen preparation for transmission and scanning electron microscopy and methods of specimen preparation for 3D electron microscopy (single particle analysis, electron tomography, array tomography and serial block-face). The results will be evaluated using electron microscopes. The course is limited to 15 participants. It is intended for Master and PhD students, young researchers, technicians, and new EM users. This course is supported by the program for large research infrastructures of the Ministry of Education, Youth and Sports within the project “National Infrastructure for Biological and Medical Imaging (Czech-BioImaging)“.
Organisers: Marie Vancová and Jana Nebesářová (Laboratory of EM, Biology Centre CAS, České Budějovice)
Invited speakers:
Vladislav Krzyžánek (Institute of Scientific Instruments CAS, Brno)
Jiří Týč (Laboratory of EM, Biology Centre CAS, České Budějovice)
Zdeno Gardian (Laboratory of EM, Biology Centre CAS, České Budějovice)
Tomáš Bílý (Macrocomplex group, South Bohemia University & Biology Centre of the CAS,České Budějovice)
Instructors:
Mgr. František Kitzberger, Mgr. Martina Tesařová, Mgr. Eva Ďurinová, Petra Masařová, Jiří Vaněček, Jana Kopecká
Programme:
Monday |
13:00 Welcome Address - Marie Vancová |
13:10 Principles of transmission electron microscopy - Tomáš Bílý |
14:00 Overview of TEM sample preparation methods - Jana Nebesářová |
14:45 Coffee break |
15:05 How to visualise macromolecules in TEM - Zdeno Gardian |
16:00 Room temperature sectioning - Jana Nebesářová |
Tuesday |
8:30 Cryo methods in sample preparation - Marie Vancová |
9:15 Principles of scanning electron microscopy - Vladislav Krzyžánek |
10:00 Coffee break |
10:20 Immunogold labeling - Marie Vancová |
11:15 Specimen preparation for SEM - Jana Nebesářová |
12:00 Lunch |
13:00 Practicals |
19:00 Course dinner |
Wednesday |
8:30 Electron tomography - Tomáš Bílý |
9:30 Serial block-face SEM, array tomography - Jiří Týč |
10:20 Coffee break |
10:40 Introduction to image processing - Zdeno Gardian |
11:25 Cellular ultrastructure and interpretation of images - Marie Vancová |
12:10 Lunch |
13:00 Practicals |
Thursday |
8:30 Practicals |
12:00 Lunch |
13:00 Practicals |
Friday |
8:30 Practicals |
12:00 Lunch |
13:00 Round table discussion |
Registration
Registration Fees
CSMS member: 6000 CZK
CSMS non-member: 7000 CZK
Industry: 10000 CZK
The course fee includes admission, the course materials, meals and coffee breaks
For registration click here
Contact: Martina Tesařová (holland@paru.cas.cz)