Serial block-face scanning electron microscopy (SBF-SEM or SBEM) is a state-of-the-art groundbreaking technique that offers volume imaging of ~1 mm3 large biological objects at nanometer-scale resolution. We have successfully applied the minimal resin (MR) embedding method to the sample preparation protocol and showed that this simple approach tackles simultaneously two major problems of...
Summary We have generated a high-confidence mitochondrial proteome (MitoTag) of the Trypanosoma brucei procyclic stage containing 1,239 proteins. For 337 of these, a mitochondrial localization had not been described before. We use the TrypTag dataset as a foundation and take advantage of the properties of the fluorescent protein tag that causes...
Wu-Chuang A., Mateos-Hernandez L., Maitre A., Rego R.O.M., Šíma R. , Porcelli S., Rakotobe S., Foucault-Simonin A., Moutailler S., Palinauskas V., Aželytė J., Šimo L., Obregon D., Cabezas-Cruz A. 2023: Microbiota perturbation by anti-microbiota vaccine reduces the colonization of Borrelia afzelii in Ixodes ricinus. Microbiome 11: 151. [IF=15.500] DOI: 10.1186/s40168-023-01599-7...
Summary Mechanistic studies on how eukaryotes ensure vertical inheritance of beneficial intracellular prokaryotes have focused mostly on highly integrated relationships. A new study by Zakharova, Tashyreva et al. reveals how a duplicated host gene impacts symbiont inheritance in a young mutualism. Main text Symbiotic relationships between...
Lyme disease (Lyme borreliosis) is a tick-borne bacterial infection that occurs in the temperate climates of North America and Eurasia. Treatment with antibiotics is effective only in the early stages of infection. Although the first human Lyme disease vaccine (LYMErix by SmithKline Beecham, Pittsburgh, PA, USA) was marketed and distributed in the USA from 1998 until 2002, it was discontinued...
Was macht Parasiten so interessant? Warum haben die Menschen Angst vor ihnen? Gibt es überhaupt einen Grund, sich vor Parasiten zu fürchten? Über- oder unterschätzen wir sie? Antworten auf diese und andere Fragen finden Sie in diesem Buch. Parasiten und ihre Lebensweise werden aus verschiedenen Blickwinkeln – medizinisch, veterinärmedizinisch, biologisch, historisch und künstlerisch –...
Ambar is at the meeting of young scientists with Nobel prize winners in Lindau and he will be talking about Short tRNA Anticodon Stem and Mutant eRF1 Allow Stop Codon Reassignment.
