Mgr. Ondřej Gahura, Ph.D.

Funkce: Odborný pracovník - Laboratoř funkční biologie protist

Kontaktní údaje

Telefon: +420 387775429
E-mail: gahura@paru.cas.cz
Místnost: PARU II, 2.05

Popis

Our research focuses on the structure, function and evolution of large macromolecular complexes in mitochondria of the parasitic protist Trypanosoma brucei.

ATP synthase

In T. brucei, the mitochondrial ATP synthase switches from the ATP producer in the insect life cycle stage to the ATP consumer maintaing the essential potential on the inner mitochondrial membrane in the infectious mammalian bloodstream form. This unique functional adaptation is accompanied by highly divergent structure. We combine structural and functional approaches to get insight into the role of ATP synthase in trypanosomal bioenergetics and mitochondrial morphology during the life cycle.

Mitochondrial ribosomes

Ribosomes, highly conserved machines fundamental for all life forms, were recently shown to undergo unexpected diversification in endosymbiotic organelles with vestigial genomes. The most divergent ribosomes are found in the mitochondria of parasitic protists from the group of Kinetoplastids, including trypanosomes. We complement the wealth of structural information on mitoribosomes and their precursors obtained by cryoEM with functional approaches to get detailed insight into lineage specific features of translation in mitochondria of trypanosomes. We aim to understand how the divergent mitoribosomes co-evolved with the peculiar context of the mitochondrial gene expression in the parasite.

Ondrej Gahura on ResearchGate

Odborný životopis

2013 - present Postdoctoral associate, Institute of Parasitology, Biology Centre CAS, Ceske Budejovice (Dr. A. Zikova)

2013-2017 Visiting scientist, Mitochondrial Biology Unit, MRC, Cambridge, UK (Sir John Walker, 18 months in total)

2012 Research associate, Faculty of Science, University of Geneva, Switzerland (Prof. F. Stutz, 3 months)

2006-2012 Research assistant, Faculty of Science, Charles University in Prague, Czech Republic (Dr. P. Folk)

2012 Ph.D. in Molecular and Cell Biology, Genetics and Virology, Charles University in Prague, Czech Republic (Dr. P. Folk)

2006 M.Sc. in Biological Sciences, Faculty of Science, Charles University in Prague, Czech Republic (Dr. P. Folk)

Výuka Epigenetika a regulace genové exprese
Přírodovědecká fakulta JČU, České Budějovice

Publikace

Celkem nalezeno: 23 záznamů
Sheikh S., Pánek T., Gahura O., Týč J., Záhonová K., Lukeš J., Eliáš M., Hashimi H. (2023) A novel group of dynamin-related proteins shared by eukaryotes and giant viruses is able to remodel mitochondria from within the matrix Molecular Biology and Evolution 40 : msad134. DOI: 10.1093/molbev/msad134 Dokumenty ke stažení: Sheikh_2023_MolBiolEvol (pdf)
Valach M., Benz C., Aguilar L.C., Gahura O., Faktorová D., Zíková A., Oeffinger M., Burger G., Gray M., Lukeš J. (2023) Miniature RNAs are embedded in an exceptionally protein-rich mitoribosome via an elaborate assembly pathway Nucleic Acids Research 51 : 6443–6460. DOI: 10.1093/nar/gkad422 Dokumenty ke stažení: Valach_2023_NucAcidRes (pdf)
Wong J., Panicucci Zíková A., Gahura O. (2023) The ancestral shape of the access proton path of mitochondrial ATP synthases revealed by a split subunit-a Molecular Biology and Evolution 40 : msad146. DOI: 10.1093/molbev/msad146
Zakharova A., Saura A., Morales J., Tashyreva D., Butenko A., Svobodová M., Poschmann G., Nandipati S., Zakharovová A., Noyvert D., Gahura O., Týč J., Stühler K., Kostygov A., Nowack E.C.M., Lukeš J., Yurchenko V. (2023) A neo-functionalized homolog of host transmembrane protein controls localization of bacterial endosymbionts in the trypanosomatid Novymonas esmeraldas Current Biology 33 : 2690-2701. DOI: 10.1016/j.cub.2023.04.060 Dokumenty ke stažení: Zakharova_2023_CB (pdf)
Gahura O., Chauhan P., Panicucci Zíková A. (2022) Mechanisms and players of mitoribosomal biogenesis revealed in trypanosomatids Trends in Parasitology 38 : 1053-1067. DOI: 10.1016/j.pt.2022.08.010
Gahura O., Mühleip A., Hierro-Yap C., Panicucci B., Jain M., Hollaus D., Slapničková M., Panicucci Zíková A., Amunts A. (2022) An ancestral interaction module promotes oligomerization in divergent mitochondrial ATP synthases Nature Communications 13 : 5989. DOI: 10.1038/s41467-022-33588-z
Cadena L., Gahura O., Panicucci B., Panicucci Zíková A., Hashimi H. (2021) Mitochondrial Contact Site and Cristae Organization System and F1FO-ATP Synthase Crosstalk Is a Fundamental Property of Mitochondrial Cristae mSphere 16 : e0032721. DOI: 10.1128/mSphere.00327-21 Dokumenty ke stažení: Cadena_2021_mSphere (pdf)
Gahura O., Hierro-Yap C., Zíková A. (2021) Redesigned and reversed: architectural and functional oddities of the trypanosomal ATP synthase Parasitology 148 : 1151–1160. DOI: 10.1017/S0031182021000202
Hierro-Yap C., Šubrtová , Gahura O., Panicucci B., Dewar C., Chinopoulos C., Schnaufer A., Zíková A. (2021) Bioenergetic consequences of FoF1-ATP synthase/ATPase deficiency in two life cycle stages of Trypanosoma brucei Journal of Biological Chemistry 296 : 100357. DOI: 10.1016/j.jbc.2021.100357
Tobiasson V., Gahura O., Aibara S., Baradaran R., Panicucci Zíková A., Amunts A. (2021) Interconnected assembly factors regulate the biogenesis of mitoribosomal large subunit Embo Journal e106292. DOI: 10.1101/2020.06.28.176446
Lukeš J., Danek P., Alejo-Valle O., Potůčková E., Gahura O., Heckl D., Stárková J., Starý J., Mejstříková E., Alberich-Jorda M., Zuna J., Trka J., Klusmann J.-H., Zaliová M. (2020) Chromosome 21 gain is dispensable for transient myeloproliferative disorder driven by a novel GATA1 mutation Leukemia 34 : 2503-2508. DOI: 10.1038/s41375-020-0769-1
Gahura O., Panicucci Zíková A. (2019) Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei Journal of visualized experiments : JoVE 22 : e58334. DOI: 10.3791/58334
Nenarokova A., Záhonová K., Krasilnikova M., Gahura O., McCulloch R., Zíková A., Yurchenko V., Lukeš J. (2019) Causes and effects of loss of classical nonhomologous end joining pathway in parasitic eukaryotes mBio 10 : e01541-19. DOI: 10.1128/mBio.01541-19 Dokumenty ke stažení: Nenarokova_et al._2019 (pdf)
Doleželová E., Terán D., Gahura O., Kotrbová Z., Procházková M., Keough D., Špaček P., Hocková D., Guddat L., Zíková A. (2018) Evaluation of the Trypanosoma brucei 6-oxopurine salvage pathway as a potential target for drug discovery PLOS Neglected Tropical Diseases DOI: 10.1371/journal.pntd.0006301
Gahura O., Panicucci B., Váchová Hana, Walker John E., Zíková A. (2018) Inhibition of F1 -ATPase from Trypanosoma brucei by its regulatory protein inhibitor TbIF1 FEBS Journal DOI: 10.1111/febs.14672
Gahura O., Šubrtová , Váchová Hana, Panicucci B., Fearnley Ian M., Harbour Michael E., Walker John E., Zíková A. (2018) The F1 -ATPase from Trypanosoma brucei is elaborated by three copies of an additional p18-subunit FEBS Journal 285 : 614-628. DOI: 10.1111/febs.14364
Montgomery M.G., Gahura O., Leslie A.G., Zíková A., Walker J.E. (2018) ATP synthase from Trypanosoma brucei has an elaborated canonical F1-domain and conventional catalytic sites. Proceedings of the National Academy of Sciences of the United States of America 115 : 2102-2107. DOI: 10.1073/pnas.1720940115
Halova Martina, Gahura O., Převorovský Martin, Cit Zdeněk, Novotný Marian, Valentova Anna, Abrhamova Katerina, Půta František, Folk Petr (2017) Nineteen complex-related factor Prp45 is required for the early stages of cotranscriptional spliceosome assembly RNA DOI: 10.1261/rna.061986.117
Panicucci B., Gahura O., Zíková A. (2017) Trypanosoma brucei TbIF1 inhibits the essential F1-ATPase in the infectious form of the parasite PloS Neglected Tropical Diseases 11 : e0005552. DOI: 10.1371/journal.pntd.0005552
Musilova Z., Rican O., Ricanova S., Jansta P., Gahura O., Novak J. (2015) Phylogeny and historical biogeography of trans-Andean cichlid fishes (Teleostei: Cichlidae) Vertebrate Zoology

Více publikací

Mgr. Ondřej Gahura, Ph.D.

KONTAKT

NAJÍT PRACOVNÍKA