Funkce: Odborný pracovník
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Laboratoř funkční biologie protist
Telefon:
+420 387775429
E-mail:
gahura@paru.cas.cz
Místnost:
PARU II, 2.05
Our research focuses on the structure, function and evolution of large macromolecular complexes in mitochondria of the parasitic protist Trypanosoma brucei.
ATP synthase
In T. brucei, the mitochondrial ATP synthase switches from the ATP producer in the insect life cycle stage to the ATP consumer maintaing the essential potential on the inner mitochondrial membrane in the infectious mammalian bloodstream form. This unique functional adaptation is accompanied by highly divergent structure. We combine structural and functional approaches to get insight into the role of ATP synthase in trypanosomal bioenergetics and mitochondrial morphology during the life cycle.
Mitochondrial ribosomes
Ribosomes, highly conserved machines fundamental for all life forms, were recently shown to undergo unexpected diversification in endosymbiotic organelles with vestigial genomes. The most divergent ribosomes are found in the mitochondria of parasitic protists from the group of Kinetoplastids, including trypanosomes. We complement the wealth of structural information on mitoribosomes and their precursors obtained by cryoEM with functional approaches to get detailed insight into lineage specific features of translation in mitochondria of trypanosomes. We aim to understand how the divergent mitoribosomes co-evolved with the peculiar context of the mitochondrial gene expression in the parasite.
Ondrej Gahura on ResearchGate
2013 - present Postdoctoral associate, Institute of Parasitology, Biology Centre CAS, Ceske Budejovice (Dr. A. Zikova)
2013-2017 Visiting scientist, Mitochondrial Biology Unit, MRC, Cambridge, UK (Sir John Walker, 18 months in total)
2012 Research associate, Faculty of Science, University of Geneva, Switzerland (Prof. F. Stutz, 3 months)
2006-2012 Research assistant, Faculty of Science, Charles University in Prague, Czech Republic (Dr. P. Folk)
2012 Ph.D. in Molecular and Cell Biology, Genetics and Virology, Charles University in Prague, Czech Republic (Dr. P. Folk)
2006 M.Sc. in Biological Sciences, Faculty of Science, Charles University in Prague, Czech Republic (Dr. P. Folk)
Epigenetika a regulace genové exprese
Přírodovědecká fakulta JČU, České Budějovice
Gahura O.,
Chauhan P.,
Panicucci Zíková A. (2022) Mechanisms and players of mitoribosomal biogenesis revealed in trypanosomatids Trends in Parasitology
38
: 1053-1067.
DOI: 10.1016/j.pt.2022.08.010
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Gahura O., Mühleip A.,
Hierro-Yap C.,
Panicucci B.,
Jain M., Hollaus D.,
Slapničková M.,
Panicucci Zíková A., Amunts A. (2022) An ancestral interaction module promotes oligomerization in divergent mitochondrial ATP synthases Nature Communications
13
: 5989.
DOI: 10.1038/s41467-022-33588-z
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Cadena L., Gahura O.,
Panicucci B.,
Panicucci Zíková A.,
Hashimi H. (2021) Mitochondrial Contact Site and Cristae Organization System and F1FO-ATP Synthase Crosstalk Is a Fundamental Property of Mitochondrial Cristae mSphere
16
: e0032721.
DOI: 10.1128/mSphere.00327-21
Dokumenty ke stažení:
Cadena_2021_mSphere (pdf)
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Gahura O.,
Hierro-Yap C.,
Zíková A. (2021) Redesigned and reversed: architectural and functional oddities of the trypanosomal ATP synthase Parasitology
148
: 1151–1160.
DOI: 10.1017/S0031182021000202
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Hierro-Yap C., Šubrtová , Gahura O.,
Panicucci B., Dewar C., Chinopoulos C., Schnaufer A.,
Zíková A. (2021) Bioenergetic consequences of FoF1-ATP synthase/ATPase deficiency in two life cycle stages of Trypanosoma brucei Journal of Biological Chemistry
296
: 100357.
DOI: 10.1016/j.jbc.2021.100357
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Tobiasson V., Gahura O., Aibara S., Baradaran R.,
Panicucci Zíková A., Amunts A. (2021) Interconnected assembly factors regulate the biogenesis of mitoribosomal large subunit Embo Journal
e106292.
DOI: 10.1101/2020.06.28.176446
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Lukeš J., Danek P., Alejo-Valle O., Potůčková E., Gahura O., Heckl D., Stárková J., Starý J., Mejstříková E., Alberich-Jorda M., Zuna J., Trka J., Klusmann J.-H., Zaliová M. (2020) Chromosome 21 gain is dispensable for transient myeloproliferative disorder driven by a novel GATA1 mutation Leukemia
34
: 2503-2508.
DOI: 10.1038/s41375-020-0769-1
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Gahura O.,
Panicucci Zíková A. (2019) Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei Journal of visualized experiments : JoVE
22
: e58334.
DOI: 10.3791/58334
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Nenarokova A., Záhonová K., Krasilnikova M., Gahura O., McCulloch R.,
Zíková A.,
Yurchenko V.,
Lukeš J. (2019) Causes and effects of loss of classical nonhomologous end joining pathway in parasitic eukaryotes mBio
10
: e01541-19.
DOI: 10.1128/mBio.01541-19
Dokumenty ke stažení:
Nenarokova_et al._2019 (pdf)
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Doleželová E., Terán D., Gahura O., Kotrbová Z., Procházková M., Keough D., Špaček P., Hocková D., Guddat L.,
Zíková A. (2018) Evaluation of the Trypanosoma brucei 6-oxopurine salvage pathway as a potential target for drug discovery PLOS Neglected Tropical Diseases
DOI: 10.1371/journal.pntd.0006301
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Gahura O.,
Panicucci B., Váchová Hana, Walker John E.,
Zíková A. (2018) Inhibition of F1 -ATPase from Trypanosoma brucei by its regulatory protein inhibitor TbIF1 FEBS Journal
DOI: 10.1111/febs.14672
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Gahura O., Šubrtová , Váchová Hana,
Panicucci B., Fearnley Ian M., Harbour Michael E., Walker John E.,
Zíková A. (2018) The F1 -ATPase from Trypanosoma brucei is elaborated by three copies of an additional p18-subunit FEBS Journal
285
: 614-628.
DOI: 10.1111/febs.14364
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Montgomery M.G., Gahura O., Leslie A.G.,
Zíková A., Walker J.E. (2018) ATP synthase from Trypanosoma brucei has an elaborated canonical F1-domain and conventional catalytic sites. Proceedings of the National Academy of Sciences of the United States of America
115
: 2102-2107.
DOI: 10.1073/pnas.1720940115
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Halova Martina, Gahura O., Převorovský Martin, Cit Zdeněk, Novotný Marian, Valentova Anna, Abrhamova Katerina, Půta František, Folk Petr (2017) Nineteen complex-related factor Prp45 is required for the early stages of cotranscriptional spliceosome assembly RNA
DOI: 10.1261/rna.061986.117
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Panicucci B., Gahura O.,
Zíková A. (2017) Trypanosoma brucei TbIF1 inhibits the essential F1-ATPase in the infectious form of the parasite PloS Neglected Tropical Diseases
11
: e0005552.
DOI: 10.1371/journal.pntd.0005552
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Musilova Z., Rican O., Ricanova S., Jansta P., Gahura O., Novak J. (2015) Phylogeny and historical biogeography of trans-Andean cichlid fishes (Teleostei: Cichlidae) Vertebrate Zoology
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Wang X., Wu F., Xie Q., Wang H., Yue Y., Gahura O., Ma S., Liu L., Cao Y., Jiao Y., Puta F., McClung CR, Xu X., Ma Ligeng (2012) SKIP is a component of the spliceosome linking alternative splicing and the circadian clock in Arabidopsis Plant Cell
DOI: 10.1105/tpc.112.100081
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Gahura O., Hammann Christian, Valentova Anna, Puta Frantisek, Folk Petr (2011) Secondary structure is required for 3' splice site recognition in yeast Nucleic Acids Research
DOI: 10.1093/nar/gkr662
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Gahura O., Abrhamova Katerina, Skurzny Michal, Valentova Anna, Valentova Anna, Munzarova Vanda, Folk Petr, Puta Frantisek (2009) Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates Journal of Cellular Biochemistry
DOI: 10.1002/jcb.21989
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