RNDr. Zdeněk Paris, Ph.D.

Funkce: Vedoucí laboratoře - Laboratoř RNA biologie prvoků

Kontaktní údaje

Telefon: +420 387775427
E-mail: parda@paru.cas.cz
Místnost: PARU II, 1. patro

Popis

Iron-sulfur clusters (ISC) are part of the active site of many enzymes and are essential for cell viability. Our study is focused on de novo synthesis of ISC in mitochondrion of Trypanosoma brucei. Additionally, thanks to wonderful collaboration with Juan D Alfonzo (Ohio State University, Columbus, Ohio, USA) we are also interested in the role of the ISC pathway on tRNA modifications and editing.

Odborný životopis

VZDĚLÁNÍ
Přírodovědecká fakulta Jihočeské univerzity v Českých Budějovicích

  • Magisterská práce: Expresní systém u Leishmania tarentolae. Obhájeno: květen 2006
  • Doktorandská práce: Identifikace nových genů, kódujících proteiny dýchacího řetězce a proteiny podílející se na tvorbě Fe-S center u Trypanosoma brucei.  Obhájeno: září 2010.

Publikace
Celkem nalezeno: 25 záznamů
Dixit S., Kessler A.C., Henderson J., Pan X., Zhao R., D'Almeida G.S., Kulkarni S., Rubio M., Hegedüsová E., Ross R.L., Limbach P., Green B.D., Paris Z., Alfonzo J. (2021) Dynamic queuosine changes in tRNA couple nutrient levels to codon choice in Trypanosoma brucei Nucleic Acids Research 49 : 12986–12999.
DOI: 10.1093/nar/gkab1204
Kulkarni S., Rubio M., Hegedüsová E., Ross R.L., Limbach P., Alfonzo J., Paris Z. (2021) Preferential import of queuosine-modified tRNAs into Trypanosoma brucei mitochondrion is critical for organellar protein synthesis Nucleic Acids Research 49 : 8247–8260.
DOI: 10.1093/nar/gkab567
Mishra A., Kaur J.N., McSkimming D.I., Hegedüsová E., Dubey A.P., Ciganda M., Paris Z., Read L. (2021) Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei. Molecular Microbiology 116 : 827–840.
DOI: 10.1111/mmi.14773
Paris Z. (2021) Nuclear tRNA export in trypanosomes: a journey full of twists and turns guided by tRNA modifications Parasitology 148 : 1219–1222.
DOI: 10.1017/S0031182021000482
Paris Z., Svobodová M., Kachale A., Horáková E., Nenarokova A., Lukeš J. (2021) A mitochondrial cytidine deaminase is responsible for C to U editing of tRNATrp to decode the UGA codon in Trypanosoma brucei RNA Biology 14 : 278–286.
DOI: 10.1080/15476286.2021.1940445

Dokumenty ke stažení:
Paris_2021_RNA (pdf)
Hegedüsová E., Kulkarni S., Burgman B., Alfonzo J., Paris Z. (2019) The general mRNA exporters Mex67 and Mtr2 play distinct roles in nuclear export of tRNAs in Trypanosoma brucei Nucleic Acids Research 47 : 8620–8631.
DOI: 31392978
Kessler A.C., Kulkarni S., Paulines M.J., Rubio M., Limbach P., Paris Z., Alfonzo J. (2018) Retrograde nuclear transport from the cytoplasm is required for tRNATyr maturation in T. brucei RNA Biology 15 : 528–536.
DOI: 10.1080/15476286.2017.1377878
Paris Z., Alfonzo J. (2018) How the intracellular partitioning of tRNA and tRNA modification enzymes affects mitochondrial function. IUBMB Life 70 : 1207–1213.
DOI: 10.1002/iub.1957
Rubio M., Gaston K., McKenney K., Fleming I., Paris Z., Limbach P., Alfonzo J. (2017) Editing and methylation at a single site by functionally interdependent activities Nature 542 : 494-497.
DOI: 10.1038/nature21396
Fleming I., Paris Z., Gaston K., Gaston K., Balakrishnan R., Fredrick K., Rubio M., Alfonzo J. (2016) A tRNA methyltransferase paralog is important for ribosome stability and cell division in Trypanosoma brucei Scientific Reports 6 : 21438.
DOI: 10.1038/srep21438
Lopes R., Silveira G., Eitler R., Vidal R., Kessler A., Hinger S., Paris Z., Alfonzo J., Polycarpo C. (2016) The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr. RNA 22 : 1190-1199.
DOI: 10.1261/rna.056242.116
Zíková A., Hampl V., Paris Z., Týč J., Lukeš J. (2016) Aerobic mitochondria of parasitic protists: diverse genomes and complex functions. Molecular and Biochemical Parasitology 209 : 46-57.
DOI: 10.1016/j.molbiopara.2016.02.007

Dokumenty ke stažení:
Zikova et al. 2016 MBP (pdf)
Horáková E., Changmai P., Paris Z., Salmon D., Lukeš J. (2015) Simultaneous depletion of Atm and Mdl rebalances cytosolic Fe-S cluster assembly but not heme import into the mitochondrion of Trypanosoma brucei FEBS Journal 282 : 4157-4175.
DOI: 10.1111/febs.13411

Dokumenty ke stažení:
Horkova et al. 2015 FEBS Journal (pdf)
Sample P., Kořený L., Paris Z., Gaston K., Rubio M., Fleming I., Hinger S., Horáková E., Limbach P., Lukeš J., Alfonzo J. (2015) A common tRNA modification at an unusual location: the discovery of wyosine biosynthesis in mitochondria Nucleic Acid Research 43 : 4262-4273.
DOI: 10.1093/nar/gkv286

Dokumenty ke stažení:
Sample_et_al_NAR_2015 (pdf)
Verner Z., Basu S., Benz C., Dixit S., Dobáková E., Faktorová D., Hashimi H., Horáková E., Huang Z., Paris Z., Pena P., Ridlon L., Týč J., Wildridge D., Zíková A., Lukeš J. (2015) The malleable mitochondrion of Trypanosoma brucei Internation Review of Cell and Molecular Biology 315 : 73-151.
DOI: 10.1016/bs.ircmb.2014.11.001

Dokumenty ke stažení:
Verner-IRCMB-2015 (pdf)
Paris Z., Horáková E., Rubio M., Sample P., Fleming I., Armocida S., Lukeš J., Alfonzo J. (2013) The T. brucei TRM5 methyltransferase plays an essential role in mitochondrial protein synthesis and function. RNA 19 : 649-658.


Dokumenty ke stažení:
2013_Paris_RNA_649 (pdf)
Rubio M., Paris Z., Gaston K., Fleming I., Sample P., Trotta C., Alfonzo J. (2013) Unusual Noncanonical Intron Editing Is Important for tRNA Splicing in Trypanosoma brucei Molecular cell 52 : 184-192.
DOI: 10.1016/j.molcel.2013.08.042
Gnipová A., Panicucci B., Paris Z., Verner Z., Horváth A., Lukeš J., Zíková A. (2012) Disparate phenotypic effects from the knockdown of various Trypanosoma brucei cytochrome c oxidase subunits. Molecular and Biochemical Parasitology 184 : 90-98.
DOI: 10.1016/j.molbiopara.2012.04.013

Dokumenty ke stažení:
2012_Gnipova_MBP_90 (pdf)
Paris Z., Hashimi H., Lun S., Alfonzo J., Lukeš J. (2011) Futile import of tRNAs and proteins into the mitochondrion of Trypanosoma brucei evansi. Molecular and Biochemical Parasitology 176 : 116–120.
DOI: 10.1016/j.molbiopara.2010.12.010

Dokumenty ke stažení:
Paris et al. 2011 MBP (pdf)
Paris Z., Changmai P., Rubio M., Zíková A., Stuart K., Alfonzo J., Lukeš J. (2010) The Fe/S Cluster assembly protein Isd11 is essential for tRNA thiolation in Trypanosoma brucei. Journal of Biological Chemistry 285 : 22394–22402.
DOI: 10.1074/jbc.M109.083774

Dokumenty ke stažení:
Paris et al. 2010 JBCh (pdf)

Více publikací

KONTAKT

Biologické centrum AV ČR, v.v.i.
Parazitologický ústav
Branišovská 1160/31
370 05 České Budějovice

NAJÍT PRACOVNÍKA