prof. RNDr. Daniel Růžek, Ph.D.

Funkce: Vědecký pracovník - Laboratoř arbovirologie

Kontaktní údaje

Telefon: +420 38777 5145
E-mail: ruzekd@paru.cas.cz
Místnost: 316

Popis

Výzkumné zaměření

Virologie: arbovirózy, zoonózy, neurovirologie

Odborný životopis

born 30. 4. 1981 in Jindřichův Hradec, Czechoslovakia

Education

2013: Associate Professor (docent) in the field of Medical Microbiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic
2005-2008: Ph.D.(Molecular and Cellular Biology and Genetics) Academy of Sciences of the Czech Republic, Institute of Parasitology, and University of South Bohemia, Faculty of Science
2007: RNDr.(Advanced Master’s degree: “Rerum Naturalium Doctor” = Doctor of Natural Sciences; Clinical Biology) University of South Bohemia, Faculty of Biological Sciences
2003-2005: MSc.(Clinical Biology; graduated with the highest honours), University of South Bohemia, Faculty of Biological Sciences
2000-2003: BSc.(Biology – Biomedical Laboratory Techniques) University of South Bohemia, Faculty of Biological Sciences
2000: High School graduation (Czech language and literature, German language, Biology, Chemistry; graduated with the highest honours), High School in Český Krumlov

Postgraduate Courses

2014: Masaryk University in Brno, Basic skills for managers (qualification course)
2013: Mendel University in Brno: Basic teaching skills in university education (qualification course)
2012: Institute of Postgraduate Education in Medicine in Prague: Methods in Laboratory Medicine (accredited qualification course)
2011: British Society for Immunology Summer School 2011, Atlantic College, St. Donat’s Castle, Wales, United Kingdom (postgraduate course)
2011: Société de Pathologie Infectieuse de Langue Française, Grenoble, France: “Acute Infectious Encephalitis” (postgraduate course)
2010: Rollins School of Public Health, Emory University, Atlanta, USA: “BSL-4 Science and Safety” (qualification course)

Employment

2013-present: Associate Professor, Department of Medical Biology, Faculty of Science, Universtity of South Bohemia, České Budějovice, Czech Republic
2012-present: Head of the Department of Virology, Head of the Research Group on Emerging Viral Diseases, Veterinary Research Institute, Brno, Czech Republic
2011-present: Research Scientist and Head of the Laboratory of Arbovirology, Institute of Parasitology, Academy of Sciences of the Czech Republic
2009-2011: Postdoctoral Scientist, Institute of Parasitology, Academy of Sciences of the Czech Republic
2008-2009: Postdoctoral Scientist, Texas Biomedical Research Institute, Department of Virology and Immunology, San Antonio, Texas, USA
2008-2013: Assistant Professor (Medical Virology), University of South Bohemia, Faculty of Science, Department of Medical Biology
2005-2009: Research Assistant, Institute of Parasitology, Academy of Sciences of the Czech Republic
2004-2008: Assistant, University of South Bohemia, Faculty of Biological Sciences, Department of Medical Biology

Research Internships

2018-2019 (3 months): Laboratoy of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan (visiting reseach scholar)
2011 (2 weeks): Laboratory of Neurovirology and Inflammation Biology, Centre for Cellular and Molecular Biology, Hyderabad, India (visiting reseach scholar)
2008-2009 (1 year): Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, USA (postdoctoral scientist)
2008 (2 weeks): Laboratoire de Physiopathologie de la Barriere Hemato-Encephalique, Faculté des Sciences Jean Perrin, Université d’Artois, Lens, France (student internship)
2006 (2 weeks): Royal (Dick) School of Veterinary Studies, Centre for Tropical Veterinary Medicine, University of Edinburgh, United Kingdom (student internship)

Professional Associations

Member of the Expert Committee for Biological Sciences of the Research, Development and Innovation Council, Office of the Government of the Czech Republic, 2014-2017 (in 2015, Chairman of the Committee)
National representative at the International Committee for Taxonomy of Viruses (ICTV)
Member of the International Scientific Working Group on Tick-borne Encephalitis
Member of the Scientific Committee of the Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
Member of the Institutional Board, Veterinary Research Institute, Brno, Czech Republic
Member of the PhD Advisory Board in the field of Medical Microbiology and Immunology, and Pathology, Faculty of Medicine, Masaryk University in Brno, Czech Republic
Member of the PhD Advisory Board in the field of Microbiology, Faculty of Science, Masaryk University in Brno, Czech Republic
Member of the Czechoslovak Society for Microbiology
Member of the American Society for Microbiology
Member and Member of the Membership Review Committee, World Society of Virology
Editorial Board Member: World Journal of Virology (since 2010), Medical Virology (Russia) (Медицинская вирусология) (since 2013), Epidemiology and Vaccinal Prevention (Russia, since 2014), Clinical and Vaccine Immunology (since 2016), Scientific Reports (since 2017), Antiviral Chemistry and Chemotherapy, Acta Virologica (since 2017), Antiviral Research (since 2019)
Associate Editor for Ticks and Tick-borne Diseases (Elsevier; since 2017)
Guest Editor: Emerging Viruses Series, Antiviral Research (Elsevier)
Guest Editor: Czech and Slovak Virology, Acta Virologica

Honors

2019: Certificate of Appreciation by the Dean of the College of Veterinary Medicine, National Chung Hsing University in Taichung, Taiwan.
2011: Travel Grant, Société de Pathologie Infectieuse de Langue Française, France
2009: Sinnecker-Kunz Award for young researchers in the field of tick-borne pathogens (Weimar, Germany)
2009: Memory Medal of the city Vyšší Brod (Czech Republic)
2009: Extraordinary Prize by the Dean for Excellent Scientific Results during Ph.D. Studies. Faculty of Science, University of South Bohemia
2006: 1st Prize, Student Scientific Work 2006, Student Grant Agency of the Faculty of Biological Sciences, University of South Bohemia
2006: Travel Grant for young researches, European Society for Clinical Virology (Birmingham, UK)
2000: Special Award, Czech National Round of the Student Scientific Work 2000, Rožnov pod Radhoštem, Czech Republic
2000: 1st Prize, Student Scientific Work 2000, South Bohemia Region, Czech Republic

Books

Růžek, D. et al. 2015. Klíšťová encefalitida (Tick-borne encephalitis), Grada Publ., Prague, 200 pp.
Růžek, D. (editor): 2011. Flavivirus Encephalitis. InTech Publ., Rijeka. 478 pp. (ISBN: 978-953-307-669-0)
Singh, S.K. & Růžek, D. (editors): 2013. Neuroviral Infections. General Principles and DNA Viruses. Taylor and Francis CRC Publ.,Boca Raton, 390 pp. (ISBN: 9781466567191)
Singh, S.K. & Růžek, D. (editors): 2013. Neuroviral Infections. RNA Viruses and Retroviruses. Taylor and Francis CRC Publ., Boca Raton, 519 pp. (ISBN: 9781466567207)
Singh, S.K. & Růžek, D. (editors): 2014. Viral hemorrhagic fevers. Taylor and Francis CRC Publ., Boca Raton, 596 pp. (ISBN: 143-988-429-3)

Book Chapters

Růžek, D., Tkachev, S.E., Yakimenko, V.V., Karan, L.S., Grubhoffer, L. Omsk hemorrhagic fever virus. In: Molecular detection of human viral pathogens, edited by: D. Liu; Taylor and Francis CRC Publ., Boca Raton, USA,231-239.
Donoso Mantke, O., Karan, L.S., Růžek, D. Tick-borne encephalitis viruses: a general overview. In: Flavivirus Encephalitis, edited by: Daniel Růžek; InTech, Rijeka,133-156.
Růžek, D., Bilski, B., Günther, G. Tick-borne encephalitis. In: Neuroviral Infections, edited by S.K. Singh and D. Růžek; Taylor and Francis CRC Publ., Boca Raton, 211-237.
Kilian, P., Danielová, V., Růžek, D. Bunyaviruses. In: Neuroviral Infections, edited by S.K. Singh and D. Růžek; Taylor and Francis CRC Publ., Boca Raton, 67-91.
Fajfr, M., Růžek, D. Laboratory diagnosis of viral hemorrhagic fever. In: Viral hemorrhagic fevers, edited by S.K. Singh and D. Růžek, Taylor and Francis CRC Publ., Boca Raton, 183-206.
Růžek, D., Yakimenko, V.V., Karan, S.K., Tkachev, S.E. Omsk hemorrhagic fever. In: Viral hemorrhagic fevers, edited by S.K. Singh and D. Růžek, Taylor and Francis CRC Publ., Boca Raton, 553-562.
Růžek, D., Holbrook, M.R., Yakimenko, V.V., Karan, S.K., Tkachev, S.E. Omsk hemorrhagic fever virus. In: Manual of security sensitive microbes and toxins, edited by D. Liu, Taylor and Francis CRC Press., Boca Raton, 193-200.
Růžek, D., Yoshii, K., Bloom, M. Gould, E.A. Tick-borne encephalitis virus – virology. In: Dobler G, Erber, W., Schmitt, H.J. Tick-borne encephalitis – an update. Global Health Press, Singapore, 2017.
Růžek, D., Yoshii, K., Bloom, M. Gould, E.A. Tick-borne encephalitis virus – virology. In: Dobler G, Erber, W., Bröker, M. Schmitt, H.J. (Eds.) The TBE Book. Second Edition. Global Health Press, Singapore, 2019. 19-41. ISBN: 978-981-14-0914-1.
Černý, J., Elsterová, J., Růžek, D., Grubhoffer, L. Vertebrate viruses in polar ecosystems. In: di Prisco G, Elster J, Huiskes A, Edwards H (editors): Life in Extreme Environments: Insights in Biological Capability. Cambridge University Press. in press.

5 most important impacted publications:

Füzik T, Formanová P, Růžek D, Yoshii K, Niedrig M, Plevka P. Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody. Nature Communications 2018 Jan 30;9(1):436.
Růžek, D., Yakimenko, V.V., Karan, L.S., Tkachev, S.E. (2010). Omsk haemorrhagic fever. The Lancet 376(9758):2104-13.
Eyer L, Kondo H, Zouharova D, Hirano M, Valdés JJ, Muto M, Kastl T, Kobayashi S, Haviernik J, Igarashi M, Kariwa H, Vaculovicova M, Cerny J, Kizek R, Kröger A, Lienenklaus S, Dejmek M, Nencka R, Palus M, Salat J, De Clercq E, Yoshii K, Ruzek D. Escape of tick-borne flavivirus from 2' -C-methylated nucleoside antivirals is mediated by a single conservative mutation in NS5 that has a dramatic effect on viral fitness. Journal of Virology 2017 Aug 16. pii: JVI.01028-17. doi: 10.1128/JVI.01028-17. (Editors‘ Choice)
Eyer L, Nencka R, Huvarová I, Palus M, Joao Alves M, Gould EA, De Clercq E, Růžek D. Nucleoside Inhibitors of Zika Virus. Journal of Infectious Diseases 2016 Sep 1;214(5):707-11. (Editors’ Choice; Highly cited according to WoS)
Eyer L, Nougairède A, Uhlířová M, Driouich JS, Zouharová D, Valdés JJ, Haviernik J, Gould EA, De Clercq E, de Lamballerie X, Ruzek D. An E460D Substitution in the NS5 Protein of Tick-Borne Encephalitis Virus Confers Resistance to the Inhibitor Galidesivir (BCX4430) and Also Attenuates the Virus for Mice. Journal of Virology. 2019 Jul 30;93(16). pii: e00367-19. (Editors’ Choice)

Výuka Lékařská virologie
Přírodovědecká fakulta Jihočeské univerzity

Patogeneze virových nákaz
Přírodovědecká fakulta Masarykovy univerzity

Publikace

Celkem nalezeno: 174 záznamů
Beránková M., Holoubek J., Hönig V., Matúšová Z., Palus M., Salát J., Krayem I., Vojtíšková J. , Svoboda P., Prančlová V., Valihrach L., Demant P., Lipoldová M., Růžek D. (2025) Genotype-driven sensitivity of mice to tick-borne encephalitis virus correlates with differential host responses in peripheral macrophages and brain Journal of Neuroinflammation 22 : 22. DOI: 10.1186/s12974-025-03354-1
Brêchet A., Kohler P., Dörr T., Grässli F., Vock M., Salát J., Růžek D., Friedl A., Vuichard-Gysin D., Croxatto A., Lienhard R., Ackermann-Gäumann R. (2025) Tick-borne encephalitis virus seroprevalence and infection incidence in Switzerland, 2020-2021. Scientific Reports 15 : 8346. DOI: 10.1038/s41598-025-92560-1
Krishnamurthy H.K., Jayaraman V., Krishna K., Wang T., Bei K., Changalath C., Matilda S., Rai A.J., Welc-Falęciak R., Pawełczyk A., Blanton L.S., Chrdle A., Fořtová A., Růžek D., Nasrallah G.K., Abu-Raddadi L.J., Al-Sadeq D.W., Abdallah M.A.A., Lilleri D., Fornara C., D'Angelo P., Furione M., Söderlund-Venermo M., Hedman K., Chochlakis D., Makridaki E., Ntoula A., Psaroulaki A., Escárcega-Ávila A., Rajasekaran J.J. (2025) A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases Scientific Reports 15 : 2527. DOI: 10.1038/s41598-024-84467-0
Kulmann M.I.R., Taborska E., Benköova B., Palus M., Drobek A., Horvat F., Pasulka J., Malik R., Salyova E., Hönig V., Pellerova M., Borsanyiova M., Nedvědová L., Štěpánek O., Bopegamage S., Růžek D., Svoboda P. (2025) Enhanced RNAi does not provide efficient innate antiviral immunity in mice Nucleic Acids Research 53 : gkae1288. DOI: 10.1093/nar/gkae1288
Lamsal A., Paulsen K.M., Diekmann M.J., Hungnes O., Alfsnes K., Quist-Paulsen E., Růžek D., Salát J., Straková P., Krogfelt K.A., Soleng A., Vikse R., Andreassen Å.K. (2025) A retrospective sero-surveillance study for antibodies against tick-borne encephalitis virus in Norway. Viruses-Basel 17 : 291. DOI: 10.3390/v17030291
Maryewski X.A., Krasilnikov M.S., Straková P., Holoubek J., Frčková T., Panina I.S., Krylov N.A., Gvozdev D.A., Denisov V.S., Semenov A. N., Lotosh N.Y., Selishcheva A.A., Gulyak E.L., Kozhemyakin G.L., Korshun V.A., Efremov R.G., Ustinov A.V., Růžek D., Eyer L., Alferova V.A. (2025) Membranea-active singlet oxygen photogenerators as a paradigm for broad-spectrum antivirals: the case of halogenated (BOron)-DIPYrromethenes ACC Applied Materials and Interfaces 17 : 4502–4528. DOI: 10.1021/acsami.4c17482
Anastasina M., Füzik T., Domanska A., Pulkkinen L.I.A., Šmerdová L., Formanová P.P., Straková P., Nováček J., Růžek D., Plevka P., Butcher S.J. (2024) The structure of immature tick-borne encephalitis virus supports the collapse model of flavivirus maturation Science Advances 10 : eadl1888. DOI: 10.1126/sciadv.adl1888
Bae J., Bednář P., Zhu R., Bong C., Bak M.S., Stainer S., Kim K., Lee J., Yoon C., Lee Y., Ojowa O.T., Lehner M., Hinterdorfer P., Růžek D., Park S., Oh Y.J. (2024) Mechanisms of plasma ozone and UV-C sterilization of SARS-CoV-2 explored through atomic force microscopy ACS Applied Materials & Interfaces 16 : 49176-49185. DOI: 10.1021/acsami.4c11057
Cervantes Rincón T., Kapoor T., Keeffe J.R., Simonelli L., Hoffmann H.H., Agudelo M., Jurado A., Peace A., Lee Y.E., Gazumyan A., Guidetti F., Cantergiani J., Cena B., Bianchini F., Tamagnini E., Moro S.G., Svoboda P., Costa F., Reis M.G., Ko A.I., Fallon B.A., Avila-Rios S., Reyes-Téran G., Rice C.M., Nussenzweig M.C., Bjorkman P.J., Růžek D., Varani L., MacDonald M.R. (2024) Human antibodies in Mexico and Brazil neutralizing tick-borne flaviviruses Cell Reports 143 : 114298. DOI: 10.1016/j.celrep.2024.114298
Fajfr M., Pajer P., Růžek D., Sleha R., Janovska S., Bohonek M., Kabickova H., Kubíčková P., Štefánik M., Straková P., Bostik P. (2024) Multicentric evaluation of sensitivity of eight commercial anti-SARS-CoV-2 antibody assays and their correlation to virus neutralization titers in seropositive subjects Scientific Reports 14 : 1421. DOI: 10.1038/s41598-024-51968-x
Fialová T., Vaculovicova M., Štefánik M., Mravec F., Burešová M., Vodova M., Rypar T., Růžek D., Hynek D., Cihalova K., Bacovsky J., Ferus M., Nejdl L. (2024) Light-triggered reactions in a new "light" of nanoparticles engineering Journal of Photochemistry and Photobiology A-Chemistry 454 : 115667. DOI: 10.1016/j.jphotochem.2024.115667
Fořtová A., Straková P., Haviernik J., Svoboda P., Bartonička T., Kvičerová J., Růžek D., Salát J. (2024) Brno loanvirus (BRNV) in bats inhabiting the urban area of Brno, Czech Republic Infection, Genetics and Evolution 121 : 105604. DOI: 10.1016/j.meegid.2024.105604
Gervais A., Marchal A., Fořtová A., Beránková M., Krbková L., Pýchová M., Salát J., Zhao S., Kerrouche N., Le Voyer T., Stiasny K., Raffl S., Schieber Pachart A., Fafi-Kremer S., Gravier S., Robbiani D.F., Abel L., MacDonald M.R., Rice C.M., Weissmann G., Kamal Eldin T., Robatscher E., Erne E.M., Pagani E., Borghesi A., Puel A., Bastard P., Velay A., Martinot M., Hansmann Y., Aberle J.H., Růžek D., Cobat A., Zhang S.Y., Casanova J.L. (2024) Autoantibodies neutralizing type I IFNs underlie severe tick-borne encephalitis in ∼10% of patients Journal of Experimental Medicine 221 : e20240637. DOI: 10.1084/jem.20240637
Holoubek J., Salát J., Kotouček J., Kastl T., Vancová M., Huvarová I., Bednář P., Bednářová K., Růžek D., Renčiuk D., Eyer L. (2024) Antiviral activity of porphyrins and porphyrin-like compounds against tick-borne encephalitis virus: blockage of the viral entry/fusion machinery by photosensitization-mediated destruction of the viral envelope Antiviral Research 221 : 105767. DOI: 10.1016/j.antiviral.2023.105767
Prančlová V., Hönig V., Zemanová M., Růžek D., Palus M. (2024) Robust CXCL10/IP-10 and CCL5/RANTES production induced by tick-borne Encephalitis virus in human brain pericytes despite weak infection International Journal of Molecular Sciences 25 : 7892. DOI: 10.3390/ijms25147892
Prančlová V., Nedvědová L., Kotounová E., Hönig V., Dvořáková M., Davídková M., Bílý T., Vancová M., Růžek D., Palus M. (2024) Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis Microbes and Infection 26 : 105383. DOI: 10.1016/j.micinf.2024.105383
Růžek D., Kaucká K. (2024) A brief tale of two pioneering moments: Europe's first discovery of Tick-Borne Encephalitis (TBE) virus beyond the Soviet Union and the largest alimentary TBE outbreak in history Ticks and Tick-borne Diseases 15 : 102314. DOI: 10.1016/j.ttbdis.2024.102314
Růžek D., Yoshii K., Bloom M.E., Gould E. (2024) TBE virology In: G. Dobler, W. Erber, M. Bröker, L. Chitimia-Dobler, H.J. Schmitt (Eds.), The TBE Book, Seventh edition. Chapter 4 . DOI: 10.33442/26613980_4-7
Šimečková P., Slavík J., Fořtová A., Huvarová I., Králiková L., Štefánik M., Svoboda P., Růžek D., Machala M. (2024) Tick-borne encephalitis virus modulates sphingolipid and phospholipid metabolism in infected human neuronal cells Microbes and Infection 26 : 105303. DOI: 10.1016/j.micinf.2024.105303
Vojnikova M., Sukupová M., Štefánik M., Straková P., Haviernik J., Kapolkova K., Gruberova E., Raskova K., Michalkova H., Švec P., Kudlickova M.P., Huvarová I., Růžek D., Salát J., Pekarik V., Eyer L., Heger Z. (2024) Nanoformulation of the Broad-Spectrum Hydrophobic Antiviral Vacuolar ATPase Inhibitor Diphyllin in Human Recombinant H-ferritin. International Journal of Nanomedicine 19 : 3907–3917. DOI: 10.2147/IJN.S452119