doc. RNDr. Daniel Růžek, Ph.D.

Funkce: Vedoucí laboratoře - Laboratoř arbovirologie

Kontaktní údaje

Telefon: +420 387775458
E-mail: ruzekd@paru.cas.cz
Místnost: 316

Popis

Výzkumné zaměření

  • Virologie: arbovirózy, zoonózy, neurovirologie

Odborný životopis

 

born 30. 4. 1981 in Jindřichův Hradec, Czechoslovakia

Education

  • 2013: Associate Professor (docent) in the field of Medical Microbiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic
  • 2005-2008: Ph.D.(Molecular and Cellular Biology and Genetics) Academy of Sciences of the Czech Republic, Institute of Parasitology, and University of South Bohemia, Faculty of Science
  • 2007: RNDr.(Advanced Master’s degree: “Rerum Naturalium Doctor” = Doctor of Natural Sciences; Clinical Biology) University of South Bohemia, Faculty of Biological Sciences
  • 2003-2005: MSc.(Clinical Biology; graduated with the highest honours), University of South Bohemia, Faculty of Biological Sciences
  • 2000-2003: BSc.(Biology – Biomedical Laboratory Techniques) University of South Bohemia, Faculty of Biological Sciences
  • 2000: High School graduation (Czech language and literature, German language, Biology, Chemistry; graduated with the highest honours), High School in Český Krumlov

Postgraduate Courses

  • 2014: Masaryk University in Brno, Basic skills for managers (qualification course)
  • 2013: Mendel University in Brno: Basic teaching skills in university education (qualification course)
  • 2012:  Institute of Postgraduate Education in Medicine in Prague: Methods in Laboratory Medicine (accredited qualification course)
  • 2011: British Society for Immunology Summer School 2011, Atlantic College, St. Donat’s Castle, Wales, United Kingdom (postgraduate course)
  • 2011:  Société de Pathologie Infectieuse de Langue Française, Grenoble, France: “Acute Infectious Encephalitis” (postgraduate course)
  • 2010: Rollins School of Public Health, Emory University, Atlanta, USA: “BSL-4 Science and Safety” (qualification course)

Employment

  • 2013-present:      Associate Professor, Department of Medical Biology, Faculty of Science, Universtity of South Bohemia, České Budějovice, Czech Republic
  • 2012-present:      Head of the Department of Virology, Head of the Research Group on Emerging Viral Diseases, Veterinary Research Institute, Brno, Czech Republic
  • 2011-present:      Research Scientist and Head of the Laboratory of Arbovirology, Institute of Parasitology, Academy of Sciences of the Czech Republic
  • 2009-2011:            Postdoctoral Scientist, Institute of Parasitology, Academy of Sciences of the Czech Republic
  • 2008-2009:            Postdoctoral Scientist, Texas Biomedical Research Institute, Department of Virology and Immunology, San Antonio, Texas, USA
  • 2008-2013:            Assistant Professor (Medical Virology), University of South Bohemia, Faculty of Science, Department of Medical Biology
  • 2005-2009:            Research Assistant, Institute of Parasitology, Academy of Sciences of the Czech Republic
  • 2004-2008:            Assistant, University of South Bohemia, Faculty of Biological Sciences, Department of Medical Biology

Research Internships

  • 2018-2019 (3 months): Laboratoy of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan (visiting reseach scholar)
  • 2011 (2 weeks):   Laboratory of Neurovirology and Inflammation Biology, Centre for Cellular and Molecular Biology, Hyderabad, India (visiting reseach scholar)
  • 2008-2009 (1 year):        Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, USA (postdoctoral scientist)
  • 2008 (2 weeks):   Laboratoire de Physiopathologie de la Barriere Hemato-Encephalique, Faculté des Sciences Jean Perrin, Université d’Artois, Lens, France (student internship)
  • 2006 (2 weeks):   Royal (Dick) School of Veterinary Studies, Centre for Tropical Veterinary Medicine, University of Edinburgh, United Kingdom (student internship)

Professional Associations

  • Member of the Expert Committee for Biological Sciences of the Research, Development and Innovation Council, Office of the Government of the Czech Republic, 2014-2017 (in 2015, Chairman of the Committee)
  • National representative at the International Committee for Taxonomy of Viruses (ICTV)
  • Member of the International Scientific Working Group on Tick-borne Encephalitis
  • Member of the Scientific Committee of the Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
  • Member of the Institutional Board, Veterinary Research Institute, Brno, Czech Republic
  • Member of the PhD Advisory Board in the field of Medical Microbiology and Immunology, and Pathology, Faculty of Medicine, Masaryk University in Brno, Czech Republic
  • Member of the PhD Advisory Board in the field of Microbiology, Faculty of Science, Masaryk University in Brno, Czech Republic
  • Member of the Czechoslovak Society for Microbiology
  • Member of the American Society for Microbiology
  • Member and Member of the Membership Review Committee, World Society of Virology
  • Editorial Board Member: World Journal of Virology (since 2010), Medical Virology (Russia) (Медицинская вирусология) (since 2013), Epidemiology and Vaccinal Prevention (Russia, since 2014), Clinical and Vaccine Immunology (since 2016), Scientific Reports (since 2017), Antiviral Chemistry and Chemotherapy, Acta Virologica (since 2017), Antiviral Research (since 2019)
  • Associate Editor for Ticks and Tick-borne Diseases (Elsevier; since 2017)
  • Guest Editor: Emerging Viruses Series, Antiviral Research (Elsevier)
  • Guest Editor: Czech and Slovak Virology, Acta Virologica

Honors

  • 2019:  Certificate of Appreciation by the Dean of the College of Veterinary Medicine, National Chung Hsing University in Taichung, Taiwan.
  • 2011: Travel Grant, Société de Pathologie Infectieuse de Langue Française, France
  • 2009: Sinnecker-Kunz Award for young researchers in the field of tick-borne pathogens (Weimar, Germany)
  • 2009: Memory Medal of the city Vyšší Brod (Czech Republic)
  • 2009:  Extraordinary Prize by the Dean for Excellent Scientific Results during Ph.D. Studies. Faculty of Science, University of South Bohemia
  • 2006:  1st Prize, Student Scientific Work 2006, Student Grant Agency of the Faculty of Biological Sciences, University of South Bohemia
  • 2006: Travel Grant for young researches, European Society for Clinical Virology (Birmingham, UK)
  • 2000: Special Award, Czech National Round of the Student Scientific Work 2000, Rožnov pod Radhoštem, Czech Republic
  • 2000: 1st Prize, Student Scientific Work 2000, South Bohemia Region, Czech Republic

Books

  • Růžek, D. et al. 2015. Klíšťová encefalitida (Tick-borne encephalitis), Grada Publ., Prague, 200 pp.
  • Růžek, D. (editor): 2011. Flavivirus Encephalitis. InTech Publ., Rijeka. 478 pp. (ISBN: 978-953-307-669-0)
  • Singh, S.K. & Růžek, D. (editors): 2013. Neuroviral Infections. General Principles and DNA Viruses. Taylor and Francis CRC Publ.,Boca Raton, 390 pp. (ISBN: 9781466567191)
  • Singh, S.K. & Růžek, D. (editors): 2013. Neuroviral Infections. RNA Viruses and Retroviruses. Taylor and Francis CRC Publ., Boca Raton, 519 pp. (ISBN: 9781466567207)
  • Singh, S.K. & Růžek, D. (editors): 2014. Viral hemorrhagic fevers. Taylor and Francis CRC Publ., Boca Raton, 596 pp. (ISBN: 143-988-429-3)

Book Chapters

  • Růžek, D., Tkachev, S.E., Yakimenko, V.V., Karan, L.S., Grubhoffer, L. Omsk hemorrhagic fever virus. In: Molecular detection of human viral pathogens, edited by: D. Liu; Taylor and Francis CRC Publ., Boca Raton, USA,231-239.
  • Donoso Mantke, O., Karan, L.S., Růžek, D. Tick-borne encephalitis viruses: a general overview. In: Flavivirus Encephalitis, edited by: Daniel Růžek; InTech, Rijeka,133-156.
  • Růžek, D., Bilski, B., Günther, G. Tick-borne encephalitis. In: Neuroviral Infections, edited by S.K. Singh and D. Růžek; Taylor and Francis CRC Publ., Boca Raton, 211-237.
  • Kilian, P., Danielová, V., Růžek, D. Bunyaviruses. In: Neuroviral Infections, edited by S.K. Singh and D. Růžek; Taylor and Francis CRC Publ., Boca Raton, 67-91.
  • Fajfr, M., Růžek, D. Laboratory diagnosis of viral hemorrhagic fever. In: Viral hemorrhagic fevers, edited by S.K. Singh and D. Růžek, Taylor and Francis CRC Publ., Boca Raton, 183-206.
  • Růžek, D., Yakimenko, V.V., Karan, S.K., Tkachev, S.E. Omsk hemorrhagic fever. In: Viral hemorrhagic fevers, edited by S.K. Singh and D. Růžek, Taylor and Francis CRC Publ., Boca Raton, 553-562.
  • Růžek, D., Holbrook, M.R., Yakimenko, V.V., Karan, S.K., Tkachev, S.E. Omsk hemorrhagic fever virus. In: Manual of security sensitive microbes and toxins, edited by D. Liu, Taylor and Francis CRC Press., Boca Raton, 193-200.
  • Růžek, D., Yoshii, K., Bloom, M. Gould, E.A. Tick-borne encephalitis virus – virology. In: Dobler G, Erber, W., Schmitt, H.J. Tick-borne encephalitis – an update. Global Health Press, Singapore, 2017.
  • Růžek, D., Yoshii, K., Bloom, M. Gould, E.A. Tick-borne encephalitis virus – virology. In: Dobler G, Erber, W., Bröker, M. Schmitt, H.J. (Eds.) The TBE Book. Second Edition. Global Health Press, Singapore, 2019. 19-41. ISBN: 978-981-14-0914-1.
  • Černý, J., Elsterová, J., Růžek, D., Grubhoffer, L. Vertebrate viruses in polar ecosystems. In: di Prisco G, Elster J, Huiskes A, Edwards H (editors): Life in Extreme Environments: Insights in Biological Capability. Cambridge University Press. in press.

5 most important impacted publications:

  • Füzik T, Formanová P, Růžek D, Yoshii K, Niedrig M, Plevka P. Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody. Nature Communications 2018 Jan 30;9(1):436.
  • Růžek, D., Yakimenko, V.V., Karan, L.S., Tkachev, S.E. (2010). Omsk haemorrhagic fever. The Lancet 376(9758):2104-13.
  • Eyer L, Kondo H, Zouharova D, Hirano M, Valdés JJ, Muto M, Kastl T, Kobayashi S, Haviernik J, Igarashi M, Kariwa H, Vaculovicova M, Cerny J, Kizek R, Kröger A, Lienenklaus S, Dejmek M, Nencka R, Palus M, Salat J, De Clercq E, Yoshii K, Ruzek D. Escape of tick-borne flavivirus from 2' -C-methylated nucleoside antivirals is mediated by a single conservative mutation in NS5 that has a dramatic effect on viral fitness. Journal of Virology 2017 Aug 16. pii: JVI.01028-17. doi: 10.1128/JVI.01028-17. (Editors‘ Choice)
  • Eyer L, Nencka R, Huvarová I, Palus M, Joao Alves M, Gould EA, De Clercq E, Růžek D. Nucleoside Inhibitors of Zika Virus. Journal of Infectious Diseases 2016 Sep 1;214(5):707-11. (Editors Choice; Highly cited according to WoS)
  • Eyer L, Nougairède A, Uhlířová M, Driouich JS, Zouharová D, Valdés JJ, Haviernik J, Gould EA, De Clercq E, de Lamballerie X, Ruzek D. An E460D Substitution in the NS5 Protein of Tick-Borne Encephalitis Virus Confers Resistance to the Inhibitor Galidesivir (BCX4430) and Also Attenuates the Virus for Mice. Journal of Virology. 2019 Jul 30;93(16). pii: e00367-19. (Editors Choice)

Výuka Lékařská virologie
Přírodovědecká fakulta Jihočeské univerzity

Patogeneze virových nákaz
Přírodovědecká fakulta Masarykovy univerzity

Publikace
Celkem nalezeno: 88 záznamů
Berčič R.L., Bányai K., Růžek D., Fehér E., Domán M., Danielová V., Bakonyi T., Nowotny N. (2019) Phylogenetic analysis of Lednice Orthobunyavirus Microorganisms 7 : 447.
DOI: 10.3390/microorganisms7100447
Dufková P., Širmarová J., Salát J., Hönig V., Palus M., Růžek D., Fooks A., Mansfield K.L., Tordo N., Jochmans D., Neyts J., Byron M., Koraka P., Osterhaus A.D.M.E., Asklepios consortium (2019) Mannitol treatment is not effective in therapy of rabies virus infection in mice Vaccine 37 : 4710–4714.
DOI: 10.1016/j.vaccine.2017.12.028
Eyer L., Fojtikova M., Nencka R., Rudolf I., Hubálek Z., Růžek D. (2019) Viral RNA-Dependent RNA Polymerase Inhibitor 7-Deaza-2'-C-Methyladenosine Prevents Death in a Mouse Model of West Nile Virus Infection Antimicrobial Agents and Chemotherapy 63 : e02093-18.
DOI: 10.1128/AAC.02093-18
Eyer L., Nougairède A., Uhlířová M., Driouich J.S., Zouharova D., Valdés J. J., Haviernik J., Gould E., De Clercq E., de Lamballerie X., Růžek D. (2019) An E460D Substitution in the NS5 Protein of Tick-Borne Encephalitis Virus Confers Resistance to the Inhibitor Galidesivir (BCX4430) and Also Attenuates the Virus for Mice. Journal of Virology 93 : pii: e00367-19.
DOI: 10.1128/JVI.00367-19
Hönig V., Palus M., Kašpar T., Majerová K., Zemanová M., Hofmannová L., Papežík P. , Šikutová S., Rettich F., Hubálek Z., Rudolf I., Votýpka J., Modrý D., Růžek D. (2019) Multiple Lineages of Usutu Virus (Flaviviridae, Flavivirus) in Blackbirds (Turdus merula) and Mosquitoes (Culex pipiens, Cx. modestus) in the Czech Republic (2016–2019) Microorganisms 7 : 568.
DOI: 10.3390/microorganisms7110568
Hönig V., Švec P., Marek L., Mrkvička T., Zubrikova D., Wittmann M., Masař O., Szturcová D., Růžek D., Pfister K., Grubhoffer L. (2019) Model of Risk of Exposure to Lyme Borreliosis and Tick-Borne Encephalitis Virus-Infected Ticks in the Border Area of the Czech Republic (South Bohemia) and Germany (Lower Bavaria and Upper Palatinate) International Journal of Environmental Research and Public Health 16 : 1173.
DOI: 10.3390/ijerph16071173
Krol E., Wandzik I., Brzuska G., Eyer L., Růžek D., Szewczyk B. (2019) Antiviral Activity of Uridine Derivatives of 2-Deoxy Sugars against Tick-Borne Encephalitis Virus Molecules 24 : 1129.
DOI: 10.3390/molecules24061129
Marosi A., Dufkova L., Forró B., Felde O., Erdélyi K., Širmarová J., Palus M., Hönig V., Salát J., Tikos R., Gyuranecz M., Růžek D., Byron M., Koraka P., Osterhaus A.D.M.E., Bakonyi T. (2019) Combination therapy of rabies-infected mice with inhibitors of pro-inflammatory host response, antiviral compounds and human rabies immunoglobulin Vaccine 37 : 4724–4735.
DOI: 10.1016/j.vaccine.2018.05.066
Pokorná Formanová P., Palus M., Salát J., Hönig V., Štefánik M., Svoboda P., Růžek D. (2019) Changes in cytokine and chemokine profiles in mouse serum and brain, and in human neural cells, upon tick-borne encephalitis virus infection Journal of neuroinflammation 16 : 205.
DOI: 10.1186/s12974-019-1596-z
Růžek D., Avšič Županc T., Borde J., Chrdle A., Eyer L., Karganova G. , Kholodilov I., Knap N., Kozlovskya L., Matveev A., Miller A., Osoldkin D.I., Överby A.K., Titukonova N. , Tkachev S., Zajkowska J. (2019) Tick-borne encephalitis in Europe and Russia: Review of pathogenesis, clinical features, therapy, and vaccines Antiviral Research 164 : 23–51.
DOI: 10.1016/j.antiviral.2019.01.014
Růžek D., Yoshii K., Bloom M.E., Gould E. (2019) Tick-borne encephalitis virus – virology. In: G. Dobler, W. Erber, H.J. Schmitt (Eds.), The TBE Book. Global Health Press, Singapore 19–41.
DOI: 10.33442/978-981-14-0914-1_2a
Zelená H., Straková P., Heroldová M., Mrázek J., Kastl T., Žákovská A., Růžek D., Smetana J., Rudolf I. (2019) Molecular epidemiology of hantaviruses in the Czech Republic Emerging Infectious Diseases 25 : 11.
DOI: 10.3201/eid2511.190449
Eyer L., Nencka R., De Clercq E., Seley-Radtke K., Růžek D. (2018) Nucleoside analogs as a rich source of antiviral agents active against arthropod-borne flaviviruses. vAntiviral Chemistry and Chemotherapy 26 : 1-28.
DOI: 10.1177/2040206618761299
Füzik T., Formanová P., Růžek D., Yoshii K., Niedrig M., Plevka P. (2018) Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody Nature Communications 9 : 436.
DOI: 10.1038/s41467-018-02882-0
Haviernik J., Štefánik M., Fojtikova M., Kali S., Noël T., Rudolf I., Hubálek Z., Růžek D., Eyer L. (2018) Arbidol (Umifenovir): A Broad-Spectrum Antiviral Drug That Inhibits Medically Important Arthropod-Borne Flaviviruses. Viruses 10 : 184.
DOI: 10.3390/v10040184
Müllerová J., Elsterová J., Černý J., Ditrich O., Žárský J., Culler L.E., Kampen H., Walther D., Coulson S., Růžek D., Grubhoffer L. (2018) No indication of arthropod-vectored viruses in mosquitoes (Diptera: Culicidae) collected on Greenland and Svalbard Polar Biology 11 : 1581–1586.
DOI: 10.1007/s00300-017-2242-9
Palus M., Sohrabi Y., Broman K.W., Strnad H., Šíma M., Růžek D., Volkova V., Slapničková M., Vojtíšková J. , Mrázková L., Salát J., Lipoldová M. (2018) A novel locus on mouse chromosome 7 that influences survival after infection with tick-borne encephalitis virus. BMC Neuroscience 19 : 39.
DOI: 10.1186/s12868-018-0438-8
Růžek D., Yoshii K., Bloom M.E., Gould E. (2018) Tick-borne encephalitis virus - Virology. In: G. Dobler, W. Erber and H.-J. Schmitt (Eds.), Tick-Borne Encephalitis (TBE) Springer 12–26.
Salát J., Formanová P., Huňady M., Eyer L., Palus M., Růžek D. (2018) Development and testing of a new tick-borne encephalitis virus vaccine candidate for veterinary use Vaccine 36 : 7257–7261.
DOI: 10.1016/j.vaccine.2018.10.034
Šebera J., Dubánková A., Sychrovský V., Růžek D., Boura E., Nencka R. (2018) The structural model of Zika virus RNA-dependent RNA polymerase in complex with RNA for rational design of novel nucleotide inhibitors Scientific Reports 8 : 11132.
DOI: 10.1038/s41598-018-29459-7

Více publikací

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