prof. RNDr. Daniel Růžek, Ph.D.

Job Position: Research Scientist - Laboratory of Arbovirology

Contact details

Phone: +420 38777 5145
E-mail: ruzekd@paru.cas.cz
Room: 316

Description

Research interests

  • virology: arboviruses, zoonoses, neurovirology
Curriculum vitae
born 30. 4. 1981 in Jindřichův Hradec, Czechoslovakia

Education

  • 2013: Associate Professor (docent) in the field of Medical Microbiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic
  • 2005-2008: Ph.D.(Molecular and Cellular Biology and Genetics) Academy of Sciences of the Czech Republic, Institute of Parasitology, and University of South Bohemia, Faculty of Science
  • 2007: RNDr.(Advanced Master’s degree: “Rerum Naturalium Doctor” = Doctor of Natural Sciences; Clinical Biology) University of South Bohemia, Faculty of Biological Sciences
  • 2003-2005: MSc.(Clinical Biology; graduated with the highest honours), University of South Bohemia, Faculty of Biological Sciences
  • 2000-2003: BSc.(Biology – Biomedical Laboratory Techniques) University of South Bohemia, Faculty of Biological Sciences
  • 2000: High School graduation (Czech language and literature, German language, Biology, Chemistry; graduated with the highest honours), High School in Český Krumlov

Postgraduate Courses

  • 2014: Masaryk University in Brno, Basic skills for managers (qualification course)
  • 2013: Mendel University in Brno: Basic teaching skills in university education (qualification course)
  • 2012:  Institute of Postgraduate Education in Medicine in Prague: Methods in Laboratory Medicine (accredited qualification course)
  • 2011: British Society for Immunology Summer School 2011, Atlantic College, St. Donat’s Castle, Wales, United Kingdom (postgraduate course)
  • 2011:  Société de Pathologie Infectieuse de Langue Française, Grenoble, France: “Acute Infectious Encephalitis” (postgraduate course)
  • 2010: Rollins School of Public Health, Emory University, Atlanta, USA: “BSL-4 Science and Safety” (qualification course)

Employment

  • 2013-present:      Associate Professor, Department of Medical Biology, Faculty of Science, Universtity of South Bohemia, České Budějovice, Czech Republic
  • 2012-present:      Head of the Department of Virology, Head of the Research Group on Emerging Viral Diseases, Veterinary Research Institute, Brno, Czech Republic
  • 2011-present:      Research Scientist and Head of the Laboratory of Arbovirology, Institute of Parasitology, Academy of Sciences of the Czech Republic
  • 2009-2011:            Postdoctoral Scientist, Institute of Parasitology, Academy of Sciences of the Czech Republic
  • 2008-2009:            Postdoctoral Scientist, Texas Biomedical Research Institute, Department of Virology and Immunology, San Antonio, Texas, USA
  • 2008-2013:            Assistant Professor (Medical Virology), University of South Bohemia, Faculty of Science, Department of Medical Biology
  • 2005-2009:            Research Assistant, Institute of Parasitology, Academy of Sciences of the Czech Republic
  • 2004-2008:            Assistant, University of South Bohemia, Faculty of Biological Sciences, Department of Medical Biology

Research Internships

  • 2018-2019 (3 months): Laboratoy of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan (visiting reseach scholar)
  • 2011 (2 weeks):   Laboratory of Neurovirology and Inflammation Biology, Centre for Cellular and Molecular Biology, Hyderabad, India (visiting reseach scholar)
  • 2008-2009 (1 year):        Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, USA (postdoctoral scientist)
  • 2008 (2 weeks):   Laboratoire de Physiopathologie de la Barriere Hemato-Encephalique, Faculté des Sciences Jean Perrin, Université d’Artois, Lens, France (student internship)
  • 2006 (2 weeks):   Royal (Dick) School of Veterinary Studies, Centre for Tropical Veterinary Medicine, University of Edinburgh, United Kingdom (student internship)

Professional Associations

  • Member of the Expert Committee for Biological Sciences of the Research, Development and Innovation Council, Office of the Government of the Czech Republic, 2014-2017 (in 2015, Chairman of the Committee)
  • National representative at the International Committee for Taxonomy of Viruses (ICTV)
  • Member of the International Scientific Working Group on Tick-borne Encephalitis
  • Member of the Scientific Committee of the Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
  • Member of the Institutional Board, Veterinary Research Institute, Brno, Czech Republic
  • Member of the PhD Advisory Board in the field of Medical Microbiology and Immunology, and Pathology, Faculty of Medicine, Masaryk University in Brno, Czech Republic
  • Member of the PhD Advisory Board in the field of Microbiology, Faculty of Science, Masaryk University in Brno, Czech Republic
  • Member of the Czechoslovak Society for Microbiology
  • Member of the American Society for Microbiology
  • Member and Member of the Membership Review Committee, World Society of Virology
  • Editorial Board Member: World Journal of Virology (since 2010), Medical Virology (Russia) (Медицинская вирусология) (since 2013), Epidemiology and Vaccinal Prevention (Russia, since 2014), Clinical and Vaccine Immunology (since 2016), Scientific Reports (since 2017), Antiviral Chemistry and Chemotherapy, Acta Virologica (since 2017), Antiviral Research (since 2019)
  • Associate Editor for Ticks and Tick-borne Diseases (Elsevier; since 2017)
  • Guest Editor: Emerging Viruses Series, Antiviral Research (Elsevier)
  • Guest Editor: Czech and Slovak Virology, Acta Virologica

Honors

  • 2019:  Certificate of Appreciation by the Dean of the College of Veterinary Medicine, National Chung Hsing University in Taichung, Taiwan.
  • 2011: Travel Grant, Société de Pathologie Infectieuse de Langue Française, France
  • 2009: Sinnecker-Kunz Award for young researchers in the field of tick-borne pathogens (Weimar, Germany)
  • 2009: Memory Medal of the city Vyšší Brod (Czech Republic)
  • 2009:  Extraordinary Prize by the Dean for Excellent Scientific Results during Ph.D. Studies. Faculty of Science, University of South Bohemia
  • 2006:  1st Prize, Student Scientific Work 2006, Student Grant Agency of the Faculty of Biological Sciences, University of South Bohemia
  • 2006: Travel Grant for young researches, European Society for Clinical Virology (Birmingham, UK)
  • 2000: Special Award, Czech National Round of the Student Scientific Work 2000, Rožnov pod Radhoštem, Czech Republic
  • 2000: 1st Prize, Student Scientific Work 2000, South Bohemia Region, Czech Republic

Books

  • Růžek, D. et al. 2015. Klíšťová encefalitida (Tick-borne encephalitis), Grada Publ., Prague, 200 pp.
  • Růžek, D. (editor): 2011. Flavivirus Encephalitis. InTech Publ., Rijeka. 478 pp. (ISBN: 978-953-307-669-0)
  • Singh, S.K. & Růžek, D. (editors): 2013. Neuroviral Infections. General Principles and DNA Viruses. Taylor and Francis CRC Publ.,Boca Raton, 390 pp. (ISBN: 9781466567191)
  • Singh, S.K. & Růžek, D. (editors): 2013. Neuroviral Infections. RNA Viruses and Retroviruses. Taylor and Francis CRC Publ., Boca Raton, 519 pp. (ISBN: 9781466567207)
  • Singh, S.K. & Růžek, D. (editors): 2014. Viral hemorrhagic fevers. Taylor and Francis CRC Publ., Boca Raton, 596 pp. (ISBN: 143-988-429-3)

Book Chapters

  • Růžek, D., Tkachev, S.E., Yakimenko, V.V., Karan, L.S., Grubhoffer, L. Omsk hemorrhagic fever virus. In: Molecular detection of human viral pathogens, edited by: D. Liu; Taylor and Francis CRC Publ., Boca Raton, USA,231-239.
  • Donoso Mantke, O., Karan, L.S., Růžek, D. Tick-borne encephalitis viruses: a general overview. In: Flavivirus Encephalitis, edited by: Daniel Růžek; InTech, Rijeka,133-156.
  • Růžek, D., Bilski, B., Günther, G. Tick-borne encephalitis. In: Neuroviral Infections, edited by S.K. Singh and D. Růžek; Taylor and Francis CRC Publ., Boca Raton, 211-237.
  • Kilian, P., Danielová, V., Růžek, D. Bunyaviruses. In: Neuroviral Infections, edited by S.K. Singh and D. Růžek; Taylor and Francis CRC Publ., Boca Raton, 67-91.
  • Fajfr, M., Růžek, D. Laboratory diagnosis of viral hemorrhagic fever. In: Viral hemorrhagic fevers, edited by S.K. Singh and D. Růžek, Taylor and Francis CRC Publ., Boca Raton, 183-206.
  • Růžek, D., Yakimenko, V.V., Karan, S.K., Tkachev, S.E. Omsk hemorrhagic fever. In: Viral hemorrhagic fevers, edited by S.K. Singh and D. Růžek, Taylor and Francis CRC Publ., Boca Raton, 553-562.
  • Růžek, D., Holbrook, M.R., Yakimenko, V.V., Karan, S.K., Tkachev, S.E. Omsk hemorrhagic fever virus. In: Manual of security sensitive microbes and toxins, edited by D. Liu, Taylor and Francis CRC Press., Boca Raton, 193-200.
  • Růžek, D., Yoshii, K., Bloom, M. Gould, E.A. Tick-borne encephalitis virus – virology. In: Dobler G, Erber, W., Schmitt, H.J. Tick-borne encephalitis – an update. Global Health Press, Singapore, 2017.
  • Růžek, D., Yoshii, K., Bloom, M. Gould, E.A. Tick-borne encephalitis virus – virology. In: Dobler G, Erber, W., Bröker, M. Schmitt, H.J. (Eds.) The TBE Book. Second Edition. Global Health Press, Singapore, 2019. 19-41. ISBN: 978-981-14-0914-1.
  • Černý, J., Elsterová, J., Růžek, D., Grubhoffer, L. Vertebrate viruses in polar ecosystems. In: di Prisco G, Elster J, Huiskes A, Edwards H (editors): Life in Extreme Environments: Insights in Biological Capability. Cambridge University Press. in press.

5 most important impacted publications:

  • Füzik T, Formanová P, Růžek D, Yoshii K, Niedrig M, Plevka P. Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody. Nature Communications 2018 Jan 30;9(1):436.
  • Růžek, D., Yakimenko, V.V., Karan, L.S., Tkachev, S.E. (2010). Omsk haemorrhagic fever. The Lancet 376(9758):2104-13.
  • Eyer L, Kondo H, Zouharova D, Hirano M, Valdés JJ, Muto M, Kastl T, Kobayashi S, Haviernik J, Igarashi M, Kariwa H, Vaculovicova M, Cerny J, Kizek R, Kröger A, Lienenklaus S, Dejmek M, Nencka R, Palus M, Salat J, De Clercq E, Yoshii K, Ruzek D. Escape of tick-borne flavivirus from 2' -C-methylated nucleoside antivirals is mediated by a single conservative mutation in NS5 that has a dramatic effect on viral fitness. Journal of Virology 2017 Aug 16. pii: JVI.01028-17. doi: 10.1128/JVI.01028-17. (Editors‘ Choice)
  • Eyer L, Nencka R, Huvarová I, Palus M, Joao Alves M, Gould EA, De Clercq E, Růžek D. Nucleoside Inhibitors of Zika Virus. Journal of Infectious Diseases 2016 Sep 1;214(5):707-11. (Editors Choice; Highly cited according to WoS)
  • Eyer L, Nougairède A, Uhlířová M, Driouich JS, Zouharová D, Valdés JJ, Haviernik J, Gould EA, De Clercq E, de Lamballerie X, Ruzek D. An E460D Substitution in the NS5 Protein of Tick-Borne Encephalitis Virus Confers Resistance to the Inhibitor Galidesivir (BCX4430) and Also Attenuates the Virus for Mice. Journal of Virology. 2019 Jul 30;93(16). pii: e00367-19. (Editors Choice)

Teaching Medical Virology
Faculty of Science, University of South Bohemia

Pathogenesis of Viral Infections
Faculty of Science, Masaryk University

Publications
Total found: 157 records
Fajfr M., Pajer P., Růžek D., Sleha R., Janovska S., Bohonek M., Kabickova H., Kubíčková P., Štefánik M., Straková P., Bostik P. (2024) Multicentric evaluation of sensitivity of eight commercial anti-SARS-CoV-2 antibody assays and their correlation to virus neutralization titers in seropositive subjects Scientific Reports 14 : 1421.
DOI: 10.1038/s41598-024-51968-x
Holoubek J., Salát J., Kotouček J., Kastl T., Vancová M., Huvarová I., Bednář P., Bednářová K., Růžek D., Renčiuk D., Eyer L. (2024) Antiviral activity of porphyrins and porphyrin-like compounds against tick-borne encephalitis virus: blockage of the viral entry/fusion machinery by photosensitization-mediated destruction of the viral envelope Antiviral Research 221 : 105767.
DOI: 10.1016/j.antiviral.2023.105767
Růžek D., Kaucká K. (2024) A brief tale of two pioneering moments: Europe's first discovery of Tick-Borne Encephalitis (TBE) virus beyond the Soviet Union and the largest alimentary TBE outbreak in history Ticks and Tick-borne Diseases 15 : 102314.
DOI: 10.1016/j.ttbdis.2024.102314
Vojnikova M., Sukupová M., Štefánik M., Straková P., Haviernik J., Kapolkova K., Gruberova E., Raskova K., Michalkova H., Švec P., Kudlickova M.P., Huvarová I., Růžek D., Salát J., Pekarik V., Eyer L., Heger Z. (2024) Nanoformulation of the Broad-Spectrum Hydrophobic Antiviral Vacuolar ATPase Inhibitor Diphyllin in Human Recombinant H-ferritin International Journal of Nanomedicine 19 : 3907–3917.
DOI: 10.2147/IJN.S452119
Bianchini F., Crivelli V., Abernathy M.E., Guerra C., Palus M., Muri J., Marcotte H., Piralla A., Pedotti M. , de Gasparo R., Simonelli L., Matkovic M., Toscano C., Biggiogero M., Calvaruso V., Svoboda P., Cervantes Rincón T., Fava T., Podešvová L., Shanbhag A.A., Celoria A., Sgrignani J., Štefánik M., Hönig V., Prančlová V., Michalcikova T., Procházka J., Guerrini G., Mehn D., Ciabattini A., Abolhassani H., Jarrossay D., Uguccioni M., Medaglini D., Pan-Hammarström Q., Calzolai L., Fernandez D. , Baldanti F., Franzetti-Pellanda A., Garzoni C., Sedlacek R., Růžek D., Varani L., Cavalli A., Barnes C.O., Robbiani D.F. (2023) Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein Science Immunology 8 : eade0958.
DOI: 10.1126/sciimmunol.ade0958
Brzuska G., Zimna M., Baranska K., Szewczyk B., Straková P., Růžek D. (2023) The influence of adjuvant type on the immunogenicity of RBD/N cocktail antigens as a vaccine candidate against SARS-CoV-2 Virus Microbiology Spectrum 18 : e0256422.
DOI: 10.1128/spectrum.02564-22
Chiffi G., Grandgirard D., Leib S.L., Chrdle A., Růžek D. (2023) Tick-borne encephalitis: a comprehensive review of the epidemiology, virology, and clinical picture Reviews in Medical Virology 33 : e2470.
DOI: 10.1002/rmv.2470
Eyer L., Seley-Radtke K., Růžek D. (2023) New directions in the experimental therapy of tick-borne encephalitis Antiviral Research 210 : 105504.
DOI: 10.1016/j.antiviral.2022.105504
Fořtová A., Barkhash A.V., Pýchová M., Krbková L., Palus M., Salát J., Růžek D. (2023) Genetic polymorphisms in innate immunity genes influence predisposition to tick-borne encephalitis Journal of NeuroVirology 29 : 699–705.
DOI: 10.1007/s13365-023-01182-8
Fořtová A., Hönig V., Salát J., Palus M., Pýchová M., Krbková L., Barkhash A.V., Kříha M.F., Chrdle A., Lipoldová M., Růžek D. (2023) Serum matrix metalloproteinase-9 (MMP-9) as a biomarker in paediatric and adult tick-borne encephalitis patients Virus Research 324 : 199020.
DOI: 10.1016/j.virusres.2022.199020
Holoubek J., Salát J., Kotouček J., Kastl T., Vancová M., Huvarová I., Bednář P., Bednářová K., Růžek D., Renčiuk D., Eyer L. (2023) Antiviral activity of porphyrins and porphyrin-like compounds against tick-borne encephalitis virus: blockage of the viral entry/fusion machinery by photosensitization-mediated destruction of the viral envelope Antiviral Research 221 : 105767.
DOI: 10.1016/j.antiviral.2023.105767
Kakuk B., Dörmő Á., Csabai Z., Kemenesi G., Holoubek J., Růžek D., Dani V.É., Dénes B., Torma G., Jakab F., Tóth G.E., Földes F.V., Zana B., Lanszki Z., Harangozó Á., Fülöp Á., Gulyás G., Mizik M., Kiss A.A., Tombácz D., Boldogkői Z. (2023) In-depth temporal transcriptome profiling of monkeypox and host cells using Nanopore sequencing Scientific Data 10 : 262.
DOI: 10.1038/s41597-023-02149-4
Kevély Á., Prančlová V., Sláviková M., Haviernik J., Hönig V., Nováková E., Palus M., Růžek D., Klempa B., Koči J. (2023) Fitness of mCherry reporter tick-borne encephalitis virus in tick experimental models Viruses-Basel 14 : 2673.
DOI: 10.3390/v14122673
Kobayashi S., Fukuda Y., Yoshii K., Thammahakin P., Maezono K., Eyer L., Růžek D., Kariwa H. (2023) Development of recombinant West Nile virus expressing mCherry reporter protein Journal of Virological Methods 37 : 114744.
DOI: 10.1016/j.jviromet.2023.114744
Mariewskaya K.A., Gvozdev D.A., Chistov A.A., Straková P., Huvarová I., Svoboda P., Kotouček J., Ivanov N.M., Krasilnikov M.S., Zhitlov M.Y., Pak A.M., Mikhnovets I.E., Nikitin T.D., Korshun V.A., Alferova V.A., Mašek J., Růžek D., Eyer L., Ustinov A.V. (2023) Membrane-targeting perylenylethynylphenols inactivate medically important coronaviruses via the singlet oxygen photogeneration mechanism Molecules 28 : 6278.
DOI: 10.3390/molecules28176278
Mikhnovets I.E., Holoubek J., Panina I.S., Kotouček J., Gvozdev D.A., Chumakov D.A., Krasilnikov M.S., Zhitlov M.Y., Gulyak E.L., Chistov A.A., Nikitin T.D., Korshun V.A., Efremov R.G., Alferova V.A., Růžek D., Eyer L., Ustinov A.V. (2023) Alkyl derivatives of perylene photosensitizing antivirals: towards understanding the influence of lipophilicity International Journal of Molecular Sciences 24 : 16483.
DOI: 10.3390/ijms242216483
Mulgaonkar N., Wang H., Mallawarachchi S., Růžek D., Martina B., Fernando S. (2023) In silico and in vitro evaluation of imatinib as an inhibitor for SARS-CoV-2 Journal of Biomolecular Structure and Dynamics 41 : 3052-3061.
DOI: 10.1080/07391102.2022.2045221
Oral C.M., Ussia M., Urso M., Salát J., Novobilský A., Štefánik M., Růžek D., Pumera M. (2023) Radiopaque nanorobots as magnetically navigable contrast agents for localized In vivo imaging of the gastrointestinal tract Advanced Healthcare Materials 12 : 2202682.
DOI: 10.1002/adhm.202202682
Penjarla S., Reddy P.Y., Penta S., Sanghvi Y., Eyer L., Štefánik M., Krátká Z., Růžek D., Banerjee S. (2023) Synthesis and antiviral activity of 2′-modified L-nucleoside analogues ChemistrySelect 8 : e202303585.
DOI: 10.1002/slct.202303585
Penjarla S., Reddy P.Y., Penta S., Sanghvi Y.S., Eyer L., Štefánik M., Růžek D., Banerjee S. (2023) Efficient synthesis of novel 2-deoxy-C-nucleosides containing oxa and thiadiazole derivatives and their biological activity Journal of Molecular Structure 1292 : 136099.
DOI: 10.1016/j.molstruc.2023.136099

More publications

CONTACT

Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

Staff search