prof. RNDr. Daniel Růžek, Ph.D.

Job Position: Research Scientist - Laboratory of Arbovirology

Contact details

Phone: +420 38777 5145
Room: 316


Research interests

  • virology: arboviruses, zoonoses, neurovirology
Curriculum vitae
born 30. 4. 1981 in Jindřichův Hradec, Czechoslovakia


  • 2013: Associate Professor (docent) in the field of Medical Microbiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic
  • 2005-2008: Ph.D.(Molecular and Cellular Biology and Genetics) Academy of Sciences of the Czech Republic, Institute of Parasitology, and University of South Bohemia, Faculty of Science
  • 2007: RNDr.(Advanced Master’s degree: “Rerum Naturalium Doctor” = Doctor of Natural Sciences; Clinical Biology) University of South Bohemia, Faculty of Biological Sciences
  • 2003-2005: MSc.(Clinical Biology; graduated with the highest honours), University of South Bohemia, Faculty of Biological Sciences
  • 2000-2003: BSc.(Biology – Biomedical Laboratory Techniques) University of South Bohemia, Faculty of Biological Sciences
  • 2000: High School graduation (Czech language and literature, German language, Biology, Chemistry; graduated with the highest honours), High School in Český Krumlov

Postgraduate Courses

  • 2014: Masaryk University in Brno, Basic skills for managers (qualification course)
  • 2013: Mendel University in Brno: Basic teaching skills in university education (qualification course)
  • 2012:  Institute of Postgraduate Education in Medicine in Prague: Methods in Laboratory Medicine (accredited qualification course)
  • 2011: British Society for Immunology Summer School 2011, Atlantic College, St. Donat’s Castle, Wales, United Kingdom (postgraduate course)
  • 2011:  Société de Pathologie Infectieuse de Langue Française, Grenoble, France: “Acute Infectious Encephalitis” (postgraduate course)
  • 2010: Rollins School of Public Health, Emory University, Atlanta, USA: “BSL-4 Science and Safety” (qualification course)


  • 2013-present:      Associate Professor, Department of Medical Biology, Faculty of Science, Universtity of South Bohemia, České Budějovice, Czech Republic
  • 2012-present:      Head of the Department of Virology, Head of the Research Group on Emerging Viral Diseases, Veterinary Research Institute, Brno, Czech Republic
  • 2011-present:      Research Scientist and Head of the Laboratory of Arbovirology, Institute of Parasitology, Academy of Sciences of the Czech Republic
  • 2009-2011:            Postdoctoral Scientist, Institute of Parasitology, Academy of Sciences of the Czech Republic
  • 2008-2009:            Postdoctoral Scientist, Texas Biomedical Research Institute, Department of Virology and Immunology, San Antonio, Texas, USA
  • 2008-2013:            Assistant Professor (Medical Virology), University of South Bohemia, Faculty of Science, Department of Medical Biology
  • 2005-2009:            Research Assistant, Institute of Parasitology, Academy of Sciences of the Czech Republic
  • 2004-2008:            Assistant, University of South Bohemia, Faculty of Biological Sciences, Department of Medical Biology

Research Internships

  • 2018-2019 (3 months): Laboratoy of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan (visiting reseach scholar)
  • 2011 (2 weeks):   Laboratory of Neurovirology and Inflammation Biology, Centre for Cellular and Molecular Biology, Hyderabad, India (visiting reseach scholar)
  • 2008-2009 (1 year):        Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, USA (postdoctoral scientist)
  • 2008 (2 weeks):   Laboratoire de Physiopathologie de la Barriere Hemato-Encephalique, Faculté des Sciences Jean Perrin, Université d’Artois, Lens, France (student internship)
  • 2006 (2 weeks):   Royal (Dick) School of Veterinary Studies, Centre for Tropical Veterinary Medicine, University of Edinburgh, United Kingdom (student internship)

Professional Associations

  • Member of the Expert Committee for Biological Sciences of the Research, Development and Innovation Council, Office of the Government of the Czech Republic, 2014-2017 (in 2015, Chairman of the Committee)
  • National representative at the International Committee for Taxonomy of Viruses (ICTV)
  • Member of the International Scientific Working Group on Tick-borne Encephalitis
  • Member of the Scientific Committee of the Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
  • Member of the Institutional Board, Veterinary Research Institute, Brno, Czech Republic
  • Member of the PhD Advisory Board in the field of Medical Microbiology and Immunology, and Pathology, Faculty of Medicine, Masaryk University in Brno, Czech Republic
  • Member of the PhD Advisory Board in the field of Microbiology, Faculty of Science, Masaryk University in Brno, Czech Republic
  • Member of the Czechoslovak Society for Microbiology
  • Member of the American Society for Microbiology
  • Member and Member of the Membership Review Committee, World Society of Virology
  • Editorial Board Member: World Journal of Virology (since 2010), Medical Virology (Russia) (Медицинская вирусология) (since 2013), Epidemiology and Vaccinal Prevention (Russia, since 2014), Clinical and Vaccine Immunology (since 2016), Scientific Reports (since 2017), Antiviral Chemistry and Chemotherapy, Acta Virologica (since 2017), Antiviral Research (since 2019)
  • Associate Editor for Ticks and Tick-borne Diseases (Elsevier; since 2017)
  • Guest Editor: Emerging Viruses Series, Antiviral Research (Elsevier)
  • Guest Editor: Czech and Slovak Virology, Acta Virologica


  • 2019:  Certificate of Appreciation by the Dean of the College of Veterinary Medicine, National Chung Hsing University in Taichung, Taiwan.
  • 2011: Travel Grant, Société de Pathologie Infectieuse de Langue Française, France
  • 2009: Sinnecker-Kunz Award for young researchers in the field of tick-borne pathogens (Weimar, Germany)
  • 2009: Memory Medal of the city Vyšší Brod (Czech Republic)
  • 2009:  Extraordinary Prize by the Dean for Excellent Scientific Results during Ph.D. Studies. Faculty of Science, University of South Bohemia
  • 2006:  1st Prize, Student Scientific Work 2006, Student Grant Agency of the Faculty of Biological Sciences, University of South Bohemia
  • 2006: Travel Grant for young researches, European Society for Clinical Virology (Birmingham, UK)
  • 2000: Special Award, Czech National Round of the Student Scientific Work 2000, Rožnov pod Radhoštem, Czech Republic
  • 2000: 1st Prize, Student Scientific Work 2000, South Bohemia Region, Czech Republic


  • Růžek, D. et al. 2015. Klíšťová encefalitida (Tick-borne encephalitis), Grada Publ., Prague, 200 pp.
  • Růžek, D. (editor): 2011. Flavivirus Encephalitis. InTech Publ., Rijeka. 478 pp. (ISBN: 978-953-307-669-0)
  • Singh, S.K. & Růžek, D. (editors): 2013. Neuroviral Infections. General Principles and DNA Viruses. Taylor and Francis CRC Publ.,Boca Raton, 390 pp. (ISBN: 9781466567191)
  • Singh, S.K. & Růžek, D. (editors): 2013. Neuroviral Infections. RNA Viruses and Retroviruses. Taylor and Francis CRC Publ., Boca Raton, 519 pp. (ISBN: 9781466567207)
  • Singh, S.K. & Růžek, D. (editors): 2014. Viral hemorrhagic fevers. Taylor and Francis CRC Publ., Boca Raton, 596 pp. (ISBN: 143-988-429-3)

Book Chapters

  • Růžek, D., Tkachev, S.E., Yakimenko, V.V., Karan, L.S., Grubhoffer, L. Omsk hemorrhagic fever virus. In: Molecular detection of human viral pathogens, edited by: D. Liu; Taylor and Francis CRC Publ., Boca Raton, USA,231-239.
  • Donoso Mantke, O., Karan, L.S., Růžek, D. Tick-borne encephalitis viruses: a general overview. In: Flavivirus Encephalitis, edited by: Daniel Růžek; InTech, Rijeka,133-156.
  • Růžek, D., Bilski, B., Günther, G. Tick-borne encephalitis. In: Neuroviral Infections, edited by S.K. Singh and D. Růžek; Taylor and Francis CRC Publ., Boca Raton, 211-237.
  • Kilian, P., Danielová, V., Růžek, D. Bunyaviruses. In: Neuroviral Infections, edited by S.K. Singh and D. Růžek; Taylor and Francis CRC Publ., Boca Raton, 67-91.
  • Fajfr, M., Růžek, D. Laboratory diagnosis of viral hemorrhagic fever. In: Viral hemorrhagic fevers, edited by S.K. Singh and D. Růžek, Taylor and Francis CRC Publ., Boca Raton, 183-206.
  • Růžek, D., Yakimenko, V.V., Karan, S.K., Tkachev, S.E. Omsk hemorrhagic fever. In: Viral hemorrhagic fevers, edited by S.K. Singh and D. Růžek, Taylor and Francis CRC Publ., Boca Raton, 553-562.
  • Růžek, D., Holbrook, M.R., Yakimenko, V.V., Karan, S.K., Tkachev, S.E. Omsk hemorrhagic fever virus. In: Manual of security sensitive microbes and toxins, edited by D. Liu, Taylor and Francis CRC Press., Boca Raton, 193-200.
  • Růžek, D., Yoshii, K., Bloom, M. Gould, E.A. Tick-borne encephalitis virus – virology. In: Dobler G, Erber, W., Schmitt, H.J. Tick-borne encephalitis – an update. Global Health Press, Singapore, 2017.
  • Růžek, D., Yoshii, K., Bloom, M. Gould, E.A. Tick-borne encephalitis virus – virology. In: Dobler G, Erber, W., Bröker, M. Schmitt, H.J. (Eds.) The TBE Book. Second Edition. Global Health Press, Singapore, 2019. 19-41. ISBN: 978-981-14-0914-1.
  • Černý, J., Elsterová, J., Růžek, D., Grubhoffer, L. Vertebrate viruses in polar ecosystems. In: di Prisco G, Elster J, Huiskes A, Edwards H (editors): Life in Extreme Environments: Insights in Biological Capability. Cambridge University Press. in press.

5 most important impacted publications:

  • Füzik T, Formanová P, Růžek D, Yoshii K, Niedrig M, Plevka P. Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody. Nature Communications 2018 Jan 30;9(1):436.
  • Růžek, D., Yakimenko, V.V., Karan, L.S., Tkachev, S.E. (2010). Omsk haemorrhagic fever. The Lancet 376(9758):2104-13.
  • Eyer L, Kondo H, Zouharova D, Hirano M, Valdés JJ, Muto M, Kastl T, Kobayashi S, Haviernik J, Igarashi M, Kariwa H, Vaculovicova M, Cerny J, Kizek R, Kröger A, Lienenklaus S, Dejmek M, Nencka R, Palus M, Salat J, De Clercq E, Yoshii K, Ruzek D. Escape of tick-borne flavivirus from 2' -C-methylated nucleoside antivirals is mediated by a single conservative mutation in NS5 that has a dramatic effect on viral fitness. Journal of Virology 2017 Aug 16. pii: JVI.01028-17. doi: 10.1128/JVI.01028-17. (Editors‘ Choice)
  • Eyer L, Nencka R, Huvarová I, Palus M, Joao Alves M, Gould EA, De Clercq E, Růžek D. Nucleoside Inhibitors of Zika Virus. Journal of Infectious Diseases 2016 Sep 1;214(5):707-11. (Editors Choice; Highly cited according to WoS)
  • Eyer L, Nougairède A, Uhlířová M, Driouich JS, Zouharová D, Valdés JJ, Haviernik J, Gould EA, De Clercq E, de Lamballerie X, Ruzek D. An E460D Substitution in the NS5 Protein of Tick-Borne Encephalitis Virus Confers Resistance to the Inhibitor Galidesivir (BCX4430) and Also Attenuates the Virus for Mice. Journal of Virology. 2019 Jul 30;93(16). pii: e00367-19. (Editors Choice)

Teaching Medical Virology
Faculty of Science, University of South Bohemia

Pathogenesis of Viral Infections
Faculty of Science, Masaryk University

Total found: 143 records
Bianchini F., Crivelli V., Abernathy M.E., Guerra C., Palus M., Muri J., Marcotte H., Piralla A., Pedotti M. , de Gasparo R., Simonelli L., Matkovic M., Toscano C., Biggiogero M., Calvaruso V., Svoboda P., Cervantes Rincón T., Fava T., Podešvová L., Shanbhag A.A., Celoria A., Sgrignani J., Štefánik M., Hönig V., Prančlová V., Michalcikova T., Procházka J., Guerrini G., Mehn D., Ciabattini A., Abolhassani H., Jarrossay D., Uguccioni M., Medaglini D., Pan-Hammarström Q., Calzolai L., Fernandez D. , Baldanti F., Franzetti-Pellanda A., Garzoni C., Sedlacek R., Růžek D., Varani L., Cavalli A., Barnes C.O., Robbiani D.F. (2023) Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein Science Immunology 8 : eade0958.
DOI: 10.1126/sciimmunol.ade0958
Brzuska G., Zimna M., Baranska K., Szewczyk B., Straková P., Růžek D. (2023) The influence of adjuvant type on the immunogenicity of RBD/N cocktail antigens as a vaccine candidate against SARS-CoV-2 Virus Microbiology Spectrum 18 : e0256422.
DOI: 10.1128/spectrum.02564-22
Eyer L., Seley-Radtke K., Růžek D. (2023) New directions in the experimental therapy of tick-borne encephalitis Antiviral Research 210 : 105504.
DOI: 10.1016/j.antiviral.2022.105504
Fořtová A., Hönig V., Salát J., Palus M., Pýchová M., Krbková L., Barkhash A.V., Kříha M.F., Chrdle A., Lipoldová M., Růžek D. (2023) Serum matrix metalloproteinase-9 (MMP-9) as a biomarker in paediatric and adult tick-borne encephalitis patients Virus Research 324 : 199020.
DOI: 10.1016/j.virusres.2022.199020
Kakuk B., Dörmő Á., Csabai Z., Kemenesi G., Holoubek J., Růžek D., Dani V.É., Dénes B., Torma G., Jakab F., Tóth G.E., Földes F.V., Zana B., Lanszki Z., Harangozó Á., Fülöp Á., Gulyás G., Mizik M., Kiss A.A., Tombácz D., Boldogkői Z. (2023) In-depth temporal transcriptome profiling of monkeypox and host cells using Nanopore sequencing Scientific Data 10 : 262.
DOI: 10.1038/s41597-023-02149-4
Kevély Á., Prančlová V., Sláviková M., Haviernik J., Hönig V., Nováková E., Palus M., Růžek D., Klempa B., Koči J. (2023) Fitness of mCherry reporter tick-borne encephalitis virus in tick experimental models Viruses-Basel 14 : 2673.
DOI: 10.3390/v14122673
Mulgaonkar N., Wang H., Mallawarachchi S., Růžek D., Martina B., Fernando S. (2023) In silico and in vitro evaluation of imatinib as an inhibitor for SARS-CoV-2 Journal of Biomolecular Structure and Dynamics 41 : 3052-3061.
DOI: 10.1080/07391102.2022.2045221
Oral C.M., Ussia M., Urso M., Salát J., Novobilský A., Štefánik M., Růžek D., Pumera M. (2023) Radiopaque nanorobots as magnetically navigable contrast agents for localized In vivo imaging of the gastrointestinal tract Advanced Healthcare Materials 12 : 2202682.
DOI: 10.1002/adhm.202202682
Penjarla S., Reddy P.Y., Penta S., Sanghvi Y.S., Eyer L., Štefánik M., Růžek D., Banerjee S. (2023) Efficient synthesis of novel 2-deoxy-C-nucleosides containing oxa and thiadiazole derivatives and their biological activity Journal of Molecular Structure 1292 : 136099.
DOI: 10.1016/j.molstruc.2023.136099
Růžek D. (2023) Editorial for the Special Issue: “Tick-Borne Encephalitis”. Microorganisms 11 : 934.
DOI: 10.3390/microorganisms11040934
Saegerman C., Humblet M.F., Leandri M., Gonzalez G., Heyman P., Sprong H., L'Hostis M., Moutailler S., Bonnet S.I., Haddad N., Boulanger N., Leib S.L., Hoch T., Thiry E., Bournez L., Kerlik J., Velay A., Jore S., Jourdain E., Gilot-Fromont E., Brugger K., Geller J., Studahl M., Knap N., Avšič-Županc T., Růžek D., Zomer T.P., Bødker R., Berger T.F.H., Martin-Latil S., De Regge N., Raffetin A., Lacour S.A., Klein M., Lernout T., Quillery E., Hubálek Z., Ruiz-Fons F., Estrada-Peňa A., Fravalo P., Kooh P., Etore F., Gossner C.M., Purse B. (2023) First expert elicitation of knowledge on possible drivers of observed increasing human cases of tick-borne encephalitis in Europe Viruses-Basel 15 : 791.
DOI: 10.3390/v15030791
Zimna M., Brzuska G., Salát J., Svoboda P., Baranska K., Szewczyk B., Růžek D., Krol E. (2023) Functional characterization and immunogenicity of a novel vaccine candidate against tick-borne encephalitis virus based on Leishmania-derived virus-like particles Antiviral Research 209 : 105511.
DOI: 10.1016/j.antiviral.2022.105511
Duraisamy G.S., Jo E., Huvarová I., Park K.P., Heger Z., Adam V., Růžek D., Windisch M.P., Miller A. (2022) Selected ginsenosides interfere efficiently with hepatitis B virus mRNA expression levels and suppress viral surface antigen secretion Heliyon 30 : e10465.
DOI: 10.1016/j.heliyon.2022.e10465
Fořtová A., Hönig V., Palus M., Salát J., Pýchová M., Krbková L., Vyhlídalová T., Kříha M.F., Chrdle A., Růžek D. (2022) Serum and cerebrospinal fluid phosphorylated neurofilament heavy subunit as a marker of neuroaxonal damage in tick-borne encephalitis Journal of General Virology 103 : 1743.
DOI: 10.1099/jgv.0.001743
Holoubek J., Bednářová K., Haviernik J., Huvarová I., Dvořáková Z., Černý J., Outlá M., Salát J., Konkol'ová E., Boura E., Růžek D. (2022) Guanine quadruplexes in the RNA genome of the tick-borne encephalitis virus: their role as a new antiviral target and in virus biology Nucleic Acids Research 50 : 4574–4600.
DOI: 10.1093/nar/gkac225
Hönig V., Kamiš J., Maršíková A., Matějková T., Stopka P., Mácová A., Růžek D., Kvičerová J. (2022) Orthohantaviruses in reservoir and atypical hosts in the Czech Republic: spillover infection and indication of virus-specific tissue tropism Microbiology Spectrum 10 : 5.
DOI: 10.1128/spectrum.01306-22
Kim J., Mayorga-Martinez C.C., Vyskočil J., Růžek D., Pumera M. (2022) Plasmonic-magnetic nanorobots for SARS-CoV-2 RNA detection through electronic readout Applied Materials Today 27 : 101402.
DOI: 10.1016/j.apmt.2022.101402
Konkolová E., Krejčová K., Eyer L., Hodek J., Zgarbová M., Fořtová A., Jirásek M., Teplý F., Reyes-Gutierrez P.E., Růžek D., Weber J., Boura E. (2022) A Helquat-like Compound as a Potent Inhibitor of Flaviviral and Coronaviral Polymerases Molecules 27 : 6.
DOI: 10.3390/molecules27061894
Kovacech B., Fialova L., Filipcik P., Skrabana R., Zilkova M., Paulenka-Ivanovova N., Kovac A., Palova D., Paulikova Rolkova G., Tomkova K., Turic Csokova N., Markova K., Skrabanova M., Sinska K., Basheer N., Majerova P., Hanes J., Parrak V., Prcina M., Cehlar O., Cente M., Piestansky J., Fresser M., Novák M., Sláviková M., Borsova K., Cabanova V., Brejová B., Vinař T., Nosek J., Klempa B., Eyer L., Hönig V., Palus M., Růžek D., Vyhlídalová T., Straková P., Mrazkova B., Zudova D., Koubkova G,, Novosadova V., Procházka J., Sedlacek R., Zilka N., Kontsekova E. (2022) Monoclonal antibodies targeting two immunodominant epitopes on the Spike protein neutralize emerging SARS-CoV-2 variants of concern Ebiomedicine 76 : 103818.
DOI: 10.1016/j.ebiom.2022.103818
Mayorga-Martinez C.C., Vyskočil J., Novotný F., Bednar P., Růžek D., Alduhaish O., Pumera M. (2022) Collective behavior of magnetic microrobots through immuno-sandwich assay: On-the-fly COVID-19 sensing Applied Materials Today 26 : 101337.
DOI: 10.1016/j.apmt.2021.101337

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Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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