Mgr. Ondřej Gahura, Ph.D.
Job Position: Postdoc - Laboratory of Functional Biology of Protists
Phone:
+420 387775429
E-mail:
gahura@paru.cas.cz
Room:
PARU II, 2.05
Our research focuses on the structure, function and evolution of large macromolecular complexes in mitochondria of the parasitic protist Trypanosoma brucei.
ATP synthase
In T. brucei, the mitochondrial ATP synthase switches from the ATP producer in the insect life cycle stage to the ATP consumer maintaing the essential potential on the inner mitochondrial membrane in the infectious mammalian bloodstream form. This unique functional adaptation is accompanied by highly divergent structure. We combine structural and functional approaches to get insight into the role of ATP synthase in trypanosomal bioenergetics and mitochondrial morphology during the life cycle.
Mitochondrial ribosomes
Ribosomes, highly conserved machines fundamental for all life forms, were recently shown to undergo unexpected diversification in endosymbiotic organelles with vestigial genomes. The most divergent ribosomes are found in the mitochondria of parasitic protists from the group of Kinetoplastids, including trypanosomes. We complement the wealth of structural information on mitoribosomes and their precursors obtained by cryoEM with functional approaches to get detailed insight into lineage specific features of translation in mitochondria of trypanosomes. We aim to understand how the divergent mitoribosomes co-evolved with the peculiar context of the mitochondrial gene expression in the parasite.
2013 - present Postdoctoral associate, Institute of Parasitology, Biology Centre ASCR, Ceske Budejovice (Dr. A. Zikova)
2013-2017 Visiting scientist, Mitochondrial Biology Unit, MRC, Cambridge, UK (Sir John Walker, 18 months in total)
2012 Research associate, Faculty of Science, University of Geneva, Switzerland (Prof. F. Stutz, 3 months)
2006-2012 Research assistant, Faculty of Science, Charles University in Prague, Czech Republic (Dr. P. Folk)
2012 Ph.D. in Molecular and Cell Biology, Genetics and Virology, Charles University in Prague, Czech Republic (Dr. P. Folk)
2006 M.Sc. in Biological Sciences, Faculty of Science, Charles University in Prague, Czech Republic (Dr. P. Folk)
Epigenetics and Gene Expression Regulation
Faculty of Science, University of South Bohemia
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Lukeš J., Danek P., Alejo-Valle O., Potůčková E., Gahura O., Heckl D., Stárková J., Starý J., Mejstříková E., Alberich-Jorda M., Zuna J., Trka J., Klusmann J.-H., Zaliová M. (2020) Chromosome 21 gain is dispensable for transient myeloproliferative disorder driven by a novel GATA1 mutation Leukemia
34
: 2503-2508. DOI: 10.1038/s41375-020-0769-1 |
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Gahura O.,
Panicucci Zíková A. (2019) Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei Journal of visualized experiments : JoVE
22
: e58334. DOI: 10.3791/58334 |
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Nenarokova A., Záhonová K., Krasilnikova M., Gahura O., McCulloch R.,
Zíková A.,
Yurchenko V.,
Lukeš J. (2019) Causes and effects of loss of classical nonhomologous end joining pathway in parasitic eukaryotes mBio
10
: e01541-19. DOI: 10.1128/mBio.01541-19 Documents to download: Nenarokova_et al._2019 (pdf) |
| Doleželová E., Terán D., Gahura O., Kotrbová Z., Procházková M., Keough D., Špaček P., Hocková D., Guddat L., Zíková A. (2018) Evaluation of the Trypanosoma brucei 6-oxopurine salvage pathway as a potential target for drug discovery PLOS Neglected Tropical Diseases DOI: 10.1371/journal.pntd.0006301 |
| Gahura O., Panicucci B., Váchová Hana, Walker John E., Zíková A. (2018) Inhibition of F1 -ATPase from Trypanosoma brucei by its regulatory protein inhibitor TbIF1 FEBS Journal DOI: 10.1111/febs.14672 |
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Gahura O.,
Šubrtová K., Váchová Hana,
Panicucci B., Fearnley Ian M., Harbour Michael E., Walker John E.,
Zíková A. (2018) The F1 -ATPase from Trypanosoma brucei is elaborated by three copies of an additional p18-subunit FEBS Journal
285
: 614-628. DOI: 10.1111/febs.14364 |
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Montgomery M.G., Gahura O., Leslie A.G.,
Zíková A., Walker J.E. (2018) ATP synthase from Trypanosoma brucei has an elaborated canonical F1-domain and conventional catalytic sites. Proceedings of the National Academy of Sciences of the United States of America
115
: 2102-2107. DOI: 10.1073/pnas.1720940115 |
| Halova Martina, Gahura O., Převorovský Martin, Cit Zdeněk, Novotný Marian, Valentova Anna, Abrhamova Katerina, Půta František, Folk Petr (2017) Nineteen complex-related factor Prp45 is required for the early stages of cotranscriptional spliceosome assembly RNA DOI: 10.1261/rna.061986.117 |
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Panicucci B., Gahura O.,
Zíková A. (2017) Trypanosoma brucei TbIF1 inhibits the essential F1-ATPase in the infectious form of the parasite PloS Neglected Tropical Diseases
11
: e0005552. DOI: 10.1371/journal.pntd.0005552 |
| Musilova Z., Rican O., Ricanova S., Jansta P., Gahura O., Novak J. (2015) Phylogeny and historical biogeography of trans-Andean cichlid fishes (Teleostei: Cichlidae) Vertebrate Zoology |
| Wang X., Wu F., Xie Q., Wang H., Yue Y., Gahura O., Ma S., Liu L., Cao Y., Jiao Y., Puta F., McClung CR, Xu X., Ma Ligeng (2012) SKIP is a component of the spliceosome linking alternative splicing and the circadian clock in Arabidopsis Plant Cell DOI: 10.1105/tpc.112.100081 |
| Gahura O., Hammann Christian, Valentova Anna, Puta Frantisek, Folk Petr (2011) Secondary structure is required for 3' splice site recognition in yeast Nucleic Acids Research DOI: 10.1093/nar/gkr662 |
| Gahura O., Abrhamova Katerina, Skurzny Michal, Valentova Anna, Valentova Anna, Munzarova Vanda, Folk Petr, Puta Frantisek (2009) Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates Journal of Cellular Biochemistry DOI: 10.1002/jcb.21989 |
