MSc. Ryan O. M. Rego, Ph.D.

Job Position: Research Scientist - Laboratory of Molecular Ecology of Vectors and Pathogens

Contact details

Phone: +420 387775497, +420 387775467
E-mail: ryanrego@paru.cas.cz
Room: 008, 327

Curriculum vitae

Current Position

Research Scientist within the Laboratory of Molecular Ecology of Vectors and Pathogens, Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic.

Research centered on adapting genetic techniques to understand the evasion strategies used by various species within Borrelia burgdorferi sensu lato, the causative agent of Lyme disease, and identification of new, common antigenic markers that could be used for detection purposes. Also interested in understanding the transmission dynamics of the old world relapsing fever borrelia , Borrelia duttonii using the tick-animal model.

Was involved in the FP7 proposal HEALTH – ANTIDotE consortium (http://www.antidote-fp7.org) for the identification and evaluation of tick vaccine candidates. P.I. of two national Czech grants and co P.I. of one bilateral grant

Postdoctoral Experience

May 2007 – April 2012 – Visiting fellow, Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana

Apr 2006 – April 2007 – Postdoctoral Fellow, Laboratory of Molecular Biology of Vectors and Pathogens, Institute of Parasitology, Biology Centre, ASCR, Ceske Budejovice, Czech Republic

Educational Background

Univ. of South Bohemia, Czech Rep.          Ph.D. Genetics & Mol. Biol.         2000–2005

  • Identified and sequenced, using a bioinformatics approach, phylogenetically conserved innate immune proteins from hard and soft ticks including defensins, integrins and lectins.
  • Localized and sequenced the first hemolymph lectin, possessing a fibrinogen domain, from a tick and involved within the tick innate immune system.
  • Comparative analyses using phylogenetic methods were done to understand the evolution of such proteins.
  • Supervisor – Prof. Vaclav Hypsa, Specialist Advisor – Prof. Libor Grubhoffer

 

Univ. of the West of England, U.K.                   M.S. Biotechnology                 1994–1995

  • Studied mixed trypanosome infections in different species of tsetse flies. Statistically identified the possibility of competition among trypanosomes within the midgut and at the time of maturation within the salivary glands.
  • Supervisors – Dr. Sue Welburn & Dr. Ian Maudlin (Present address: Univ. of Edinburgh)

 

St.Joseph’s College of Arts&Sc.(B.U.), India   B.S. Microbiology                    1990–1993

 

Invited lectures at international conferences

  • XXV International Congress of Entomology (ICE 2016, Orlando, Florida, USA) September 25–30, 2016,
  • 2019 Entomological Society of America Meeting (St. Louis, Missouri, USA).

 

Requested lectures and seminars abroad

  • International Symposium on Tick-Borne Pathogens and Disease ITPD 2017 (Vienna, Austria) 24 to 26 September 2017
  • Université de Neuchâtel, Institut de Biologie, Switzerland – 15th Dec 2016
  • University Hospital Frankfurt, Institute of Medical Microbiology and Infection Control, Germany  – 8th Nov. 2016
  • Veterinary Sciences, University of Arizona, Tuscon, Arizona , USA – 7th March 2016

Supported Grant Projects in the Czech Republic

  • P. I. - GACR (Grant Agency of the Czech Republic): Delineating the role of Borrelia afzelii genes required for dissemination within an infected tick during feeding (2017–2019). Total amount awarded
  • P.I. -TACR (Technology Agency of the Czech Republic): Identification of novel antigens/diagnostic markers for the development of diagnostic tests for early diagnosis of Lyme Disease in Europe (2017-2018)
  • Co-P.I. Ministry of Education mobility grant between Czehc Republic and Austria. (2019-2010)

Applied research

  • Connected with the Grant from TACR –GAMA to identify serological markers that would help detect the early onset of Lyme disease.

Mentoring

2019 – three B.Sc. students have obtained student grants from the University of South Bohemia with Dr. Rego as the supervisor.

2017– one B.Sc. student obtained student grant from the University of South Bohemia with Dr. Rego as the supervisor

2018 – two M.Sc. students obtained and successfully defended student grants obtained from the University of South Bohemia with Dr. Rego as the supervisor

Defended theses: two M.S. projects and ten B.Sc. projects, Faculty of Science, University of South Bohemia

Currently: One Postdoc, One Ph.D. student and 5 B.Sc. students, Faculty of Science, University of South Bohemia.

Editorial Duties

Guest Editor for Frontiers in Cellular and Infection Microbiology : Research Topic- Biological Drivers of Vector-Pathogen Interactions (2019)

Journal Referee

The American journal of tropical medicine and hygiene, Gene Therapy, FEMS Microbiology Letters, Journal of Bacteriology, Ticks and Tick-borne Diseases, PLoS Neglected Tropical Diseases, Parasites & Vectors, Zoonotic Diseases abd Public Health, Frontiers in Microbiology, Frontiers in Celluar and Infection Microbiology, Pathogens

Organization Member

  • American Society of Microbiology
  • European Society of Clinical Microbiology & Infectious Diseases (ESCMID)

 

Publications (Last 5 years):

  • Hodžić A., Mateos-Hernández L., Leschnik M., Alberdi P., Rego R. O. M., Contreras M., Villar M., de la Fuente J., Cabezas-Cruz A., Duscher G. G. 2019 Tick Bites Induce Anti-α-Gal Antibodies in Dogs Vaccines 7, 114

  • Rego ROM, Trentelman JJA, Anguita J, Nijhof AM, Sprong H, Klempa B, Hajdusek O, Tomás-Cortázar J, Azagi T, Strnad M, Knorr S, Sima R, Jalovecka M, Fumačová Havlíková S, Ličková M, Sláviková M, Kopacek P, Grubhoffer L, Hovius JW. 2019 Counterattacking the tick bite: towards a rational design of anti-tick vaccines targeting pathogen transmission. Parasit Vectors. 2019 May 14;12(1):229.

  • Rodríguez-Ruano S.M., Škochová V., Rego R. O. M., Schmidt J.O., Roachell W., Hypša V., Nováková E. 2018 Microbiomes of North American Triatominae: The Grounds for Chagas Disease Epidemiology Frontiers in Microbiology 9 : 1167.

  • Strnad M., Oh Y., Vancová M., Hinterdorfer P., Grubhoffer L., Rego R. O. M. 2018 Interaction forces between the extracellular matrix and variants of borrelial decorin binding protein A probed by atomic force microscopy. FEBS Open Bio 8 : 203.

  • Věchtová P., Štěrbová J., Štěrba J., Vancová M., Rego R. O. M., Selinger M., Strnad M., Golovchenko M., Rudenko N., Grubhoffer L. 2018 A bite so sweet: the glycobiology interface of tick-host-pathogen interactions Parasites & Vectors 11 : 594.

  • Belli A, Sarr A, Rais O, Rego R.O.M., Voordouw MJ. 2017 Ticks infected via co-feeding transmission can transmit Lyme borreliosis to vertebrate hosts. Scientific Reports 7(1):5006.

  • Cabezas-Cruz A, Estrada-Peña A, Rego ROM, De la Fuente J. 2017 Tick-Pathogen Ensembles: Do Molecular Interactions Lead Ecological Innovation? Frontiers in Cellular and Infection Microbiology 7:74  

  • de la Fuente J, Antunes S, Bonnet S, Cabezas-Cruz A, Domingos AG, Estrada-Peña A, Johnson N, Kocan KM, Mansfield KL, Nijhof AM, Papa A, Rudenko N, Villar M, Alberdi P, Torina A, Ayllón N, Vancova M, Golovchenko M, Grubhoffer L, Caracappa  S, Fooks AR, Gortazar C, Rego R.O.M. 2017 Tick-Pathogen Interactions and Vector Competence: Identification of Molecular Drivers for Tick-Borne Diseases. Frontiers in Cellular and Infection Microbiology 7:114.      

  • Strnad M, Hönig V, Růžek D, Grubhoffer L, Rego R.O.M. 2017 Europe-Wide Meta-Analysis of Borrelia burgdorferi Sensu Lato Prevalence in Questing Ixodes ricinus Ticks. Applied and Environmental Microbiology 83(15)  

  • Vancová M, Rudenko N, Vaněček J, Golovchenko M, Strnad M, Rego R.O.M., Tichá L, Grubhoffer L, Nebesářová J. 2017 Pleomorphism and Viability of the Lyme Disease Pathogen Borrelia burgdorferi Exposed to Physiological Stress Conditions: A Correlative Cryo-Fluorescence and Cryo-Scanning Electron Microscopy Study. Frontiers in Microbiology 8:596  

  • Strnad M., Elsterová J., Schrenková J., Vancova M., Rego R.O.M., Grubhoffer L., and Nebesářová J. 2015 Correlative cryo-fluorescence and cryo-scanning electron microscopy as a straightforward tool to study host-pathogen interactions.  Scientific Reports - 2015 Dec 10;5:1802

  • Tonk, M., Cabezas-Cruz, A., Valdes, J.J., Rego, R.O.M. et al. Ixodes ricinus defensins attack distantly-related pathogens Dev Comp Immunol. 2015 53(2):358–65

  • Tonk, M., Cabezas-Cruz, A., Valdes, J.J., Rego, R.O.M. et al., Defensins from the tick Ixodes scapularis are effective against phtopathogenic fungi and the human bacterial pathogen Listeria grayi Parasit Vectors. 2014 Dec 3;7(1):554

  • Chrudimska, T., Cerovsky, V., Slaninova, J., Rego, R.O.M. and Grubhoffer, L. Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes. Dev Comp Immunol. 2014 46(2):165-70

  • Rego, R.O.M., Bestor, A., Stefka, J and Rosa, P.A. Population Bottlenecks During the Infectious Cycle of the Lyme Disease Spirochete Borrelia burgdorferi. PLoS One. 2014 Jun 30;9(6):e101009

  • Sprong, H., Tentelman, J., Seemann, I., Grubhoffer, L., Rego, R.O.M. et al., ANTIDotE: Anti-tick Vaccines to Prevent Tick-borne Diseases in Europe. Parasites and Vectors 2014 Feb 21;7:77

  • Tonk, M., Cabezas-Cruz, A., Valdes, J.J., Rego, R.O.M. et al., Identification and partial characterisation of new members of the Ixodes ricinus defensin family. 2014 Gene 540(2):146–5

Publications
Total found: 30 records
Genné D., Rossel M., Sarr A., Battilotti F., Rais O., Rego R. O. M., Voordouw M.J. (2021) Competition between strains of Borrelia afzelii in the host tissues and consequences for transmission to ticks The ISME Journal 2021 : 2021.
DOI: 10.1038/s41396-021-00939-5
Strnad M., Oh Y.J., Vancová M., Hain L., Salo J., Grubhoffer L., Nebesářová J., Hytönen J., Hinterdorfer P., Rego R. O. M. (2021) Nanomechanical mechanisms of Lyme disease spirochete motility enhancement in extracellular matrix Communications Biology 4 : 268 .
DOI: 10.1038/s42003-021-01783-1
Gobl J., Sinha D., Šíma R., Perner J., Kopáček P., Valdés J. J., Rego R. O. M., Cabezas-Cruz A. (2020) Histone Methyltransferase DOT1L Is Involved in Larval Molting and Second Stage Nymphal Feeding in Ornithodoros moubata Vaccines 8 : 157.
DOI: 10.3390/vaccines8020157
Kotsarenko K., Věchtová P., Lieskovská J., Fussy Z., Cabral-de-Mello D.C., Rego R. O. M., Alberdi P., Collins M., Bell-Sakyi L., Štěrba J., Grubhoffer L. (2020) Karyotype changes in long-term cultured tick cell lines Scientific Reports 10 : 13443.
DOI: 10.1038/s41598-020-70330-5
Rego R. O. M., Lopez J.E., Cabezas-Cruz A. (2020) Editorial: Biological Drivers of Vector–Pathogen Interactions Frontiers in Cellular and Infection Microbiology 10 : 609495.
DOI: 10.3389/fcimb.2020.609495
Strnad M., Grubhoffer L., Rego R. O. M. (2020) Novel targets and strategies to combat borreliosis Applied Microbiology and Biotechnology 104 : 1915-1925.
DOI: 10.1007/s00253-020-10375-8
Strnad M., Rego R. O. M. (2020) The need to unravel the twisted nature of the Borrelia burgdorferi sensu lato complex across Europe Microbiology 166 : 428–435.
DOI: 10.1099/mic.0.000899
Hodžić A., Mateos-Hernández L., Leschnik M., Alberdi P., Rego R. O. M., Contreras M., Villar M., de la Fuente J., Cabezas-Cruz A., Duscher G. G. (2019) Tick Bites Induce Anti-α-Gal Antibodies in Dogs Vaccines 7 : 114.
DOI: 10.3390/vaccines7030114
Rego R. O. M., Trentelman J.J., Anguita J., Nijhof A., Sprong H., Klempa B., Hajdušek O., Tomás-Corázar J., Azagi T., Strnad M., Knorr S. , Šíma R., Jalovecká M., Havlíková S., Ličková M., Sláviková M., Kopáček P., Grubhoffer L., Hovius J. (2019) Counterattacking the tick bite: towards a rational design of anti-tick vaccines targeting pathogen transmission Parasites & Vectors 12 : 229.
DOI: 10.1186/s13071-019-3468-x
Rodríguez-Ruano S.M., Škochová V., Rego R. O. M., Schmidt J.O., Roachell W., Hypša V., Nováková E. (2018) Microbiomes of North American Triatominae: The Grounds for Chagas Disease Epidemiology Frontiers in Microbiology 9 : 1167.
DOI: 10.3389/fmicb.2018.01167
Strnad M., Oh Y., Vancová M., Hinterdorfer P., Grubhoffer L., Rego R. O. M. (2018) Interaction forces between the extracellular matrix and variants of borrelial decorin binding protein A probed by atomic force microscopy. FEBS Open Bio 8 : 203.
Věchtová P., Štěrbová J., Štěrba J., Vancová M., Rego R. O. M., Selinger M., Strnad M., Golovchenko M., Rudenko N., Grubhoffer L. (2018) A bite so sweet: the glycobiology interface of tick-host-pathogen interactions Parasites & Vectors 11 : 594.
DOI: 10.1186/s13071-018-3062-7
Belli A., Sarr A., Rais O., Rego R. O. M., Voordouw M.J. (2017) Ticks infected via co-feeding transmission can transmit Lyme borreliosis to vertebrate hosts Scientific Reports 7 : 5006.
DOI: 10.1038/s41598-017-05231-1
de la Fuente J., Antunes S., Bonnet S., Domingos A., Estrada-Peňa A., Johnson N., Kocan K., Mansfield K., Nijhof A., Papa A., Rudenko N., Villar M., Alberdi P., Torina A., Ayllón N., Vancová M., Golovchenko M., Grubhoffer L., Caracappa S., Fooks A., Gortazar C., Rego R. O. M. (2017) Tick-pathogen interactions and vector competence: identification of molecular drivers for tick-borne diseases Frontiers in Cellular and Infection Microbiology 7 : 114.
DOI: 10.3389/fcimb.2017.00114
Estrada-Peňa A., Rego R. O. M., de la Fuente J. (2017) Tick-pathogen ensembles: do molecular interactions lead ecological innovation? Frontiers in Cellular and Infection Microbiology 7 : 74.
DOI: 10.3389/fcimb.2017.00074
Hönig Mondeková H., Šíma R., Urbanová V., Kovář V., Rego R. O. M., Grubhoffer L., Kopáček P., Hajdušek O. (2017) Characterization of Ixodes ricinus Fibrinogen-Related Proteins (Ixoderins) Discloses Their Function in the Tick Innate Immunity Frontiers in Cellular and Infection Microbiology 7 : 509.
DOI: 10.3389/fcimb.2017.00509
Strnad M., Hönig V., Růžek D., Grubhoffer L., Rego R. O. M. (2017) Europe-wide meta-analysis of Borrelia burgdorferi sensu lato prevalence in questing Ixodes ricinus ticks Applied and Environmental Microbiology 83 : e00609-17.
DOI: 10.1128/AEM.00609-17
Vancová M., Rudenko N., Vaněček J., Golovchenko M., Strnad M., Rego R. O. M., Tichá L., Grubhoffer L., Nebesářová J. (2017) Pleomorphism and viability of the Lyme disease pathogen Borrelia burgdorferi exposed to physiological stress conditions: a correlative cryo-fluorescence and cryo-scanning electron microscopy study Frontiers in Microbiology 8 : 596.
DOI: 10.3389/fmicb.2017.00596
Strnad M., Elsterová J., Schrenková J., Vancová M., Rego R. O. M., Grubhoffer L., Nebesářová J. (2015) Correlative cryo-fluorescence and cryo-scanning electron microscopy as a straightforward tool to study host-pathogen interactions Scientific Reports 5 : 18029.
DOI: 10.1038/srep18029
Tonk M., Valdés J. J., Grubhoffer L., Rego R. O. M., Estrada-Peňa A., Vilcinskas A., Kotsyfakis M., Rahnamaeian M. (2015) Ixodes ricinus defensins attack distantly-related pathogens Developmental & Comparative Immunology 53 : 358-365.
DOI: 10.1016/j.dci.2015.08.001

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Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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