MSc. Ryan O. M. Rego, Ph.D.
Job Position: Research Scientist - Laboratory of Molecular Ecology of Vectors and Pathogens
+420 387775497, +420 387775467
Room: 008, 327
- May 2007 – April 2012: Visiting fellow, Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana
- Apr 2006 – April 2007: Postdoctoral Fellow, Laboratory of Molecular Biology of Vectors and Pathogens, Institute of Parasitology, Biology Centre, ASCR, Ceske Budejovice, Czech Republic
University of South Bohemia, Czech Rep. Ph.D. Genetics & Mol. Biol. 2000-2005
Supervisor – Prof. Vaclav Hypsa, Specialist Advisor – Prof. Libor Grubhoffer
- Identified and sequenced, using a bioinformatics approach, phylogenetically conserved innate immune proteins from hard and soft ticks including defensins, integrins and lectins.
- Localized and sequenced the first hemolymph lectin, possessing a fibrinogen domain, from a tick and involved within the tick innate immune system.
- Comparative analyses using phylogenetic methods were done to understand the evolution of such proteins.
University of the West of England, U.K. M.S. Biotechnology 1994-1995
Supervisors – Dr. Sue Welburn & Dr. Ian Maudlin (Present address: Univ. of Edinburgh)
- Studied mixed trypanosome infections in different species of tsetse flies. Statistically identified the possibility of competition among trypanosomes within the midgut and at the time of maturation within the salivary glands.
St.Joseph’s College of Arts&Sc.(B.U.), India B.S. Microbiology 1990-1993
Supervisor Dr. Betty Daniels, Dept. of Microbiology
- Identified Urinary Tract Infections from urine samples of residents of slum-dwelling areas in Bangalore, India.
- 100 Hours Honors Programme:
1990 – 1991 Entomology (Study into beneficial insects)
1991 – 1992 Communication Skills and Individual Development
1992 – 1993 Genetics
Awarded Special Certificate for project work on Syndromes – Chromosomal Abnormalities and Aberrations
Betsor, A., Rego, R.O.M., Tilly, K. and Rosa, P. Competitive advantage of Borrelia burgdorferi with outer-surface protein BBA03 during tick-mediated infection of the mammalian host. Infection and Immunity 2012; 80(10): 3501-11
Dulebohn, D., Bestor, A., Rego, R.O.M., Stewart, P. and Rosa, P.A. The Borrelia burgdorferi linear plasmid lp38 is dispensable for completion of the mouse-tick infectious cycle. Infection AND Immunity 2011 Sep; 79(9): 3510-7
Rego, R.O.M., Bestor, A., and Rosa, P.A. Defining the plasmid-encoded restriction-modification systems of the Lyme disease spirochete Borrelia burgdorferi. J. Bact. 2011 Mar; 193(5):1161-71
Grubhoffer, L., Rego, R.O.M., Hajdušek, O., Hypša, V., Kovář, V., Rudenko N. and Oliver J.H. Jr. 2008 Tick lectins and fibrinogen-related proteins. (In Ticks: Biology, Disease and Control, Cambridge University Press) 2008: 127-142
Rego, R.O.M., Kovář, V., Kopáček, P., Weise, C., Man, P., Sauman, I., and Grubhoffer, L. The tick plasma lectin, Dorin M, is a fibrinogen-related molecule. Insect Biochem. Mol. Biol. 2006. 36 (4) 291 – 299
Rego, R.O.M., Hajdušek, O., Kovář, V., Kopáček, P., Grubhoffer, L., and Hypša, V. Molecular cloning and comparative analysis of fibrinogen-related proteins from the soft tick Ornithodoros moubata and the hard tick Ixodes ricinus . Insect Biochem. Mol. Biol. 2005. 35 (9) 991 – 1004
Rudenko N., Golovchenko M., Rego R., Marten. E., and Grubhoffer L.: Two defensins from the hard tick Ixodes ricinus: Does the site of expression determine the function? Proceedings of the 5th Int, Conf. Ticks and Tick-borne Pathogens, Neuchâtel, Switzerland 29.8. - 2.9., 2005, No. 229, pp. 195-199.
Hypša,V., Tietz,D., Zrzavy,J., Rego,R.O.M., Galvao,C., Jurberg,J. Phylogeny and Biogeography of triatominae (Hemiptera:Reduvidae): Molecular Evidence of a New World origin of the Asiatic clade. Molecular Phylogenetics and Evolution 2002 Jun;23(3):447-57
Rego, R.O.M., Bestor, A, and Rosa, P.A. Understanding the in-vivo population dynamics of the Lyme disease pathogen, Borrelia burgdorferi: In vivo veritas - Gordon Research Conference – Biology of Spirochetes January 22-27, 2012
Rego, R.O.M., Bestor, A, and Rosa, P.A. Understanding the in-vivo population dynamics of the Lyme disease pathogen, Borrelia burgdorferi: In vivo veritas. - FASEB Summer Research Conference – Microbial Pathogenesis : Mechanisms of Infectious Disease. July 24th-29th, 2011
Rego, R.O.M., Bestor, A, and Rosa, P.A. – Understanding the in-vivo population dynamics of the Lyme disease pathogen, Borrelia burgdorferi: In vivo veritas. Intermountain West Immunology Workshop August 5th-6th, 2010
Rego, R.O.M., Bestor A. and Rosa, P.A. Plasmid-encoded barriers to foreign DNA and genetic manipulation of Borrelia burgdorferi- 110th General Meeting, ASM- May 23rd- 27th, 2010
Rego, R.O.M, Bestor, A. and Rosa, P.A. Enhancing the transformation of Borrelia burgdorferi by in vivo modification of shuttle vector DNA – FASEB Summer Research Conference – Microbial Pathogenesis : Mechanisms of Infectious Disease. July 19th-24th, 2009
Workshops, Courses and Committees
Intermountain West Immunology Workshop August 5th-6th, 2010
Teaching course ‘Scientists teaching science’ conducted by the Office of Intramural Training and Development, NIH – 2008 (8 week course)
Member of the organizing committee for the 3rd Annual NIAID Postdoctoral Retreat in Washington DC, 2009
Committee member of the Research Fellows Organization at the Rocky Mountain Laboratories, NIAID, NIH - 2008 – 2010
Completed Animal Users Course conducted at the Rocky Mountain Laboratories, NIAID, NIH – 2007 (Participant of yearly refresher course)
Attended the Conference on ‘Pattern Recognition Proteins and Receptors’ held in Trest , Czech Republic, 2003.
Involved in the organization of the ‘Biology of Disease Vectors Course’ held by the Institute of Parasitology, České Budejovice, Czech Republic, 2001.
Apr 2006 – April 2007 Post-doctoral Researcher, Institute of Parasitology, České Budějovice, Czech Republic.
Mar 1999 – Jul 2004 Worked as a Research Assistant at the Institute of Parasitology, České Budějovice, Czech Republic.
Aug 1996 – Nov. 1998 Position – Scientist - Bhat Biotech India (P) Ltd., Bangalore, India.