prof. Ing. Miroslav Oborník, Ph.D.
Job Position: Head of Laboratory - Laboratory of Evolutionary Protistology
Phone:
+420 387775464
E-mail:
obornik@paru.cas.cz
Room:
236
Research interests
Evolution of protists and related algae.
Education and employment
- 1985-1991: Student Ing. (MSc) , Faculty of Agriculture Ceské Budejovice, Agricultural University - Prague, specialization "Genetic Engineering and Plant Breeding"
- 1991: Fellowship , Faculty of Agriculture Ceské Budejovice, Agricultural University - Prague, Dept. of Plant Production, Division of Plant Protection
- 1991-1995: PhD student , Faculty of Agriculture, University of South Bohemia, Dept. of Plant Production, Division of Plant Protection
- 10/1995 - 12/1996: Civil service
- 1998 - 2000: Assistant Professor , Faculty of Agriculture, University of South Bohemia, Dept. of Plant Production, Division of Plant Protection
- 1998 - : Research scientist , Institute of Parasitology, Academy of Sciences of the Czech Republic, Division of Molecular Parasitology
- 2000 - : Research scientist, head of the laboratory , Institute of Parasitology (IP), Academy of Sciences of the Czech Republic, and University of South Bohemia (USB), Faculty of Biological Sciences - Laboratory of Molecular Taxonomy (joint laboratory of IP and USB)
- 2005-2008 Assistent Professor in Molecular and Cell Biology and Genetics, Faculty of Science, University of South Bohemia, České Budějovice
- 2008- present: Senior Research Scientist, Biology Centre ASCR, Institute of Parasitology
- 2008- present - Associate Professor in Molecular and Cell Biology and Genetics, Faculty of Science, University of South Bohemia, České Budějovice
Foreign programs
- May - August 1993: Wageningen Agricultural University, Dept. of Entomology, The Netherlands(Dr. R. Stouthamer)
- December 1994 - September 1995: Wageningen Agricultural University, Dept. of Entomology, The Netherlands(Dr. R. Stouthamer)
- April - May 1997: University of Horticulture and Food Industry, Budapest, Hungary
- November 2002 - November 2003: University of British Columbia, Department of Botany, Vancouver, Canada(Dr. B.R. Green)
Research priorities
MO is currently working on the evolution of secondary endosymbiosis. Many unicellular photoautotrophic eukaryotes (such as diatoms, heterokonts, cryptophytes, haptophytes, euglenoids) possess complex photosynthetic organelles that evolved during the process of secondary endosymbiogenesis. Such secondary (or complex) plastids are surrounded by three or four membranes. Nuclear-encoded proteins are postraslationally imported into the complex plastid via secretory pathway. We have participated on the annotation of the genome of diatom Thalassiosira pseudonana , particularly on the search of nuclear-encoded plastid targeted proteins, their in silico localization and phylogeny.
Complex plastid is also present in apicomplexan parasites, however, it is not photosynthetic in this case. The size of the apicomplexan plastid genome is extremely small (35 kb) thus offering a limited number of genes for phylogenetic analysis. Moreover, the sequences of apicoplast genes are highly AT rich and rapidly evolving. Although it is evident that the apicoplast and its genome is plastid-derived, our detailed phylogenetic analysis of amino acid and nucleotide sequences of selected apicoplast ribosomal protein genes rpl2 , rpl14 and rps12 show their possible mitochondrial origin. Based on our results, a hypothesis is proposed that apicoplasts might contain both plastid and mitochondrial genes, thus constituting a hybrid assembly.
MO is also involved in phylogenetic projets concerning molecular phylogeny of protozoa, e.g. microsporidia, apicomplexan parasites and kinteoplastids.
Complex plastid is also present in apicomplexan parasites, however, it is not photosynthetic in this case. The size of the apicomplexan plastid genome is extremely small (35 kb) thus offering a limited number of genes for phylogenetic analysis. Moreover, the sequences of apicoplast genes are highly AT rich and rapidly evolving. Although it is evident that the apicoplast and its genome is plastid-derived, our detailed phylogenetic analysis of amino acid and nucleotide sequences of selected apicoplast ribosomal protein genes rpl2 , rpl14 and rps12 show their possible mitochondrial origin. Based on our results, a hypothesis is proposed that apicoplasts might contain both plastid and mitochondrial genes, thus constituting a hybrid assembly.
MO is also involved in phylogenetic projets concerning molecular phylogeny of protozoa, e.g. microsporidia, apicomplexan parasites and kinteoplastids.
Hobby
- Playing the guitar (music group - Smutný Karel )
Introduction to Bioinformatics
Faculty of Science, University of South Bohemia
Introduction to Bioinformatics exercises
Faculty of Science, University of South Bohemia
Molecular Taxonomy
Faculty of Agriculture, University of South Bohemia
| Total found: 77 records
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Brunson J.K., McKinnie S.M.K., Chekan J.R., McCrow J.P., Miles Z.D., Bertrand E.M., Bielinski V.A., Luhavaya H., Oborník M., Smith G.J., Hutchins D.A., Allen A.E., Moore B.S. (2018) Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom. Science
361
: 1356–1358. DOI: 10.1126/science.aau0382 Documents to download: Brunson_et al._2018_Science (pdf) |
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Füssy Z., Oborník M. (2018) Complex Endosymbioses I: From Primary to Complex Plastids, Multiple Independent Events. In: E. Maréchal (Ed.), Methods in Molecular Biology. Humana Press, New York
1829
: 17–35. DOI: 10.1007/978-1-4939-8654-5_2 Documents to download: Fussy and Obornik_2018_Chapter (pdf) |
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McQuaid J.B., Kustka A.B., Oborník M.,
Horák A., Mccrow J., Karas B.J., Zheng H., Kindeberg T., Andersson A.J., Barbeau K.A., Allen A. (2018) Carbonate-sensitive phytotransferrin controls high-affinity iron uptake in diatoms Nature
555
: 534-537. DOI: 10.1038/nature25982 |
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Oborník M. (2018) The Birth of Red Complex Plastids: One, Three, or Four Times? Trends in Parasitology
34
: 11. DOI: 10.1016/j.pt.2018.09.001 |
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Sharaf A., Oborník M., Hammad A., El-Afifi S., Marei E. (2018) Characterization and comparative genomic analysis of virulent and temperate Bacillus megaterium bacteriophages PeerJ
6
: e5687. DOI: 10.7717/peerj.5687 |
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Vazač J.,
Füssy Z., Hladová I.,
Killi S., Oborník M. (2018) Ploidy and Number of Chromosomes in the Alveolate Alga Chromera velia. Protist
169
: 53-63. DOI: 10.1016/j.protis.2017.12.001 |
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Záhonová K.,
Füssy Z., Birčák E., Novák Vanclová A.M.G., Klimeš V., Vesteg M., Krajčovič J., Oborník M., Eliáš M. (2018) Peculiar features of the plastids of the colourless alga Euglena longa and photosynthetic euglenophytes unveiled by transcriptome analyses Scientific Reports
8
: 17012. DOI: 10.1038/s41598-018-35389-1 |
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Füssy Z.,
Masařová P.,
Kručinská J.,
Esson H. J., Oborník M. (2017) Budding of the alveolate alga Vitrella brassicaformis resembles sexual and asexual processes in apicomplexan parasites Protist
168
: 80-91. DOI: 10.1016/j.protis.2016.12.001 |
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Füssy Z., Oborník M. (2017) Reductive Evolution of Apicomplexan Parasites from Phototrophic Ancestors. In: Pontarotti P. (Ed.), Evolutionary Biology: Self/Nonself Evolution, Species and Complex Traits Evolution, Methods and Concepts. Springer, Cham
217-236. DOI: 10.1007/978-3-319-61569-1_12 |
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Füssy Z., Oborník M. (2017) Chromerids and their plastids Advances in Botanical Research
84
: 187-218. DOI: 10.1016/bs.abr.2017.07.001 |
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Sobotka R.,
Esson H. J.,
Koník P., Trsková E., Moravcová L.,
Horák A.,
Dufková P., Oborník M. (2017) Extensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans Scientific Reports
7
: 13214. |
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Tomčala A., Kyselová V.,
Schneedorferová I., Opekarová I., Moos M., Urajová P.,
Kručinská J., Oborník M. (2017) Separation and identification of lipids in the photosynthetic cousins of Apicomplexa Chromera velia and Vitrella brassicaformis. Journal of Separation Science
40
: 3402-3413. DOI: 10.1002/jssc.201700171 |
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Votýpka J.,
Modrý D., Oborník M., Šlapeta J.,
Lukeš J. (2017) Apicomplexa. In: J.M. Archibald, A.G.B. Simpson and C.H. Slamovits (Eds.), Handbook of the Protists Springer
1-58. DOI: 10.1007/978-3-319-32669-6_20-1 Documents to download: CHAPTER Votypka et al. 2017 (pdf) |
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Cihlář J.,
Füssy Z.,
Horák A., Oborník M. (2016) Evolution of the Tetrapyrrole Biosynthetic Pathway in Secondary Algae: Conservation, Redundancy and Replacement PLoS ONE
11
: e0166338. DOI: 10.1371/journal.pone.0166338 |
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Oborník M.,
Kručinská J.,
Esson H. J. (2016) Life cycles of chromerids resemble those of colpodellids and apicomplexan parasites Perspectives in Phycology
3
: 21-27. DOI: 10.1127/pip/2016/0038 |
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Záhonová K.,
Füssy Z., Oborník M., Eliáš M.,
Yurchenko V. (2016) RuBisCO in Non-Photosynthetic Alga Euglena longa: Divergent Features, Transcriptomic Analysis and Regulation of Complex Formation PLoS ONE
11
: e0158790. DOI: 10.1371/journal.pone.0158790 Documents to download: Zahonova et al. 2016 PloS ONE (pdf) |
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Borovička J., Oborník M., Stříbrný J., Noordeloos M., Parra Sánchez L., Gryndler M. (2015) Phylogenetic and chemical studies in the potential psychotropic species complex of Psilocybe atrobrunnea with taxonomic and nomenclatoral notes Persoonia
34
: 11-13. DOI: 10.3767/003158515X685283 |
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Doležalová J., Oborník M.,
Hajdušková E.,
Jirků M., Petrželková K., Bolechová P., Cutillas C., Callejón R., Jaroš J., Beránková Z.,
Modrý D. (2015) How many species of whipworms do we share? Whipworms from man and other primates form two phylogenetic lineages Folia Parasitologica
62
: 063. DOI: 10.14411/fp.2015.063 Documents to download: Dolezalova et al FP 2015 (pdf) |
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Flegontov P.,
Janouškovec J., Lai H.,
Jirků M.,
Hajdušková E.,
Tomčala A., Otto T.D., Keeling P., Pain A., Oborník M.,
Lukeš J. (2015) Divergent Mitochondrial Respiratory Chains in Phototrophic Relatives of Apicomplexan Parasites. Molecular Biology and Evolution
32
: 1115-1131. DOI: 10.1093/molbev/msv021 Documents to download: Flegontov et al. 2015 MBE (pdf) |
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Koblížek M., Moulisová V., Muroňová M., Oborník M. (2015) Horizontal transfers of two types of puf operons among phototrophic members of the Roseobacter clade Folia Microbiologica
60
: 37-43. DOI: 10.1007/s12223-014-0337-z |
