prof. Ing. Miroslav Oborník, Ph.D.

Job Position: Head of Laboratory - Laboratory of Evolutionary Protistology

Contact details

Phone: +420 387775464
Room: 236


Research interests

Evolution of protists and related algae.

Curriculum vitae


Education and employment

  • 1985-1991: Student Ing. (MSc) , Faculty of Agriculture Ceské Budejovice, Agricultural University - Prague, specialization "Genetic Engineering and Plant Breeding"
  • 1991: Fellowship , Faculty of Agriculture Ceské Budejovice, Agricultural University - Prague, Dept. of Plant Production, Division of Plant Protection
  • 1991-1995: PhD student , Faculty of Agriculture, University of South Bohemia, Dept. of Plant Production, Division of Plant Protection
  • 10/1995 - 12/1996: Civil service
  • 1998 - 2000: Assistant Professor , Faculty of Agriculture, University of South Bohemia, Dept. of Plant Production, Division of Plant Protection
  • 1998 - : Research scientist , Institute of Parasitology, Academy of Sciences of the Czech Republic, Division of Molecular Parasitology
  • 2000 - : Research scientist, head of the laboratory , Institute of Parasitology (IP), Academy of Sciences of the Czech Republic, and University of South Bohemia (USB), Faculty of Biological Sciences - Laboratory of Molecular Taxonomy (joint laboratory of IP and USB)
  • 2005-2008 Assistent Professor in Molecular and Cell Biology and Genetics, Faculty of Science, University of South Bohemia, České Budějovice
  • 2008- present: Senior Research Scientist, Biology Centre ASCR, Institute of Parasitology
  • 2008- present - Associate Professor in Molecular and Cell Biology and Genetics, Faculty of Science, University of South Bohemia, České Budějovice

Foreign programs

  • May - August 1993: Wageningen Agricultural University, Dept. of Entomology, The Netherlands(Dr. R. Stouthamer)
  • December 1994 - September 1995: Wageningen Agricultural University, Dept. of Entomology, The Netherlands(Dr. R. Stouthamer)
  • April - May 1997: University of Horticulture and Food Industry, Budapest, Hungary
  • November 2002 - November 2003: University of British Columbia, Department of Botany, Vancouver, Canada(Dr. B.R. Green)

Research priorities

MO is currently working on the evolution of secondary endosymbiosis. Many unicellular photoautotrophic eukaryotes (such as diatoms, heterokonts, cryptophytes, haptophytes, euglenoids) possess complex photosynthetic organelles that evolved during the process of secondary endosymbiogenesis. Such secondary (or complex) plastids are surrounded by three or four membranes. Nuclear-encoded proteins are postraslationally imported into the complex plastid via secretory pathway. We have participated on the annotation of the genome of diatom Thalassiosira pseudonana , particularly on the search of nuclear-encoded plastid targeted proteins, their in silico localization and phylogeny.
Complex plastid is also present in apicomplexan parasites, however, it is not photosynthetic in this case. The size of the apicomplexan plastid genome is extremely small (35 kb) thus offering a limited number of genes for phylogenetic analysis. Moreover, the sequences of apicoplast genes are highly AT rich and rapidly evolving. Although it is evident that the apicoplast and its genome is plastid-derived, our detailed phylogenetic analysis of amino acid and nucleotide sequences of selected apicoplast ribosomal protein genes rpl2 , rpl14 and rps12 show their possible mitochondrial origin. Based on our results, a hypothesis is proposed that apicoplasts might contain both plastid and mitochondrial genes, thus constituting a hybrid assembly.
MO is also involved in phylogenetic projets concerning molecular phylogeny of protozoa, e.g. microsporidia, apicomplexan parasites and kinteoplastids.


Teaching Introduction to Bioinformatics
Faculty of Science, University of South Bohemia

Introduction to Bioinformatics exercises
Faculty of Science, University of South Bohemia

Molecular Taxonomy
Faculty of Agriculture, University of South Bohemia

Total found: 92 records
Oborník M. (2020) Photoparasitism as an Intermediate State in theEvolution of Apicomplexan Parasites Trends in Parasitology 36 : 727-734.
DOI: 10.1016/
Sato S., Nanjappa D., Dorrell R.G., Vieira F.R.J., Kazamia E., Tirichine L., Veluchamy A., Heilig R., Aury J.M., Jaillon O., Wincker P., Füssy Z., Oborník M., Muñoz-Gómez S.A., Mann D., Bowler C., Zingone A. (2020) Genome-enabled Phylogenetic and Functional Reconstruction of an Araphid Pennate Diatom Plagiostriata Sp. CCMP470, Previously Assigned as a Radial Centric Diatom, and Its Bacterial Commensal Scientific Reports 10 : 9449.
DOI: 10.1038/s41598-020-65941-x
Tomčala A., Michálek J., Schneedorferová I., Füssy Z., Gruber A., Vancová M., Oborník M. (2020) Fatty Acid Biosynthesis in Chromerids Biomolecules 10 : 1102.
DOI: 10.3390/biom10081102
Cihlář J., Füssy Z., Oborník M. (2019) Evolution of tetrapyrrole pathway in eukaryotic phototrophs Advances in Botanical Research 90 : 273–309.
DOI: 10.1016/bs.abr.2018.12.003
Coale T.H., Moosburner M., Horák A., Oborník M., Barbeau K.A., Allen A.E. (2019) Reduction-dependent siderophore assimilation in a model pennate diatom Proceedings of the National Academy of Sciences of United States of America 116 : 23609-23617.
DOI: 10.1073/pnas.1907234116
Füssy Z., Faitová T., Oborník M. (2019) Subcellular Compartments Interplay for Carbon and Nitrogen Allocation in Chromera velia and Vitrella brassicaformis. Genome biology and evolution. 11 : 1765-1779.
DOI: 10.1093/gbe/evz123
Jirsová D., Füssy Z., Richtová J., Gruber A., Oborník M. (2019) Morphology, ultrastructure, and mitochondrial genome of the marine non-photosynthetic bicosoecid Cafileria marina gen. et sp. nov. Microorganisms 7 : 240.
DOI: 10.3390/microorganisms7080240
Oborník M. (2019) In the beginning was the word: How terminology drives our understanding of endosymbiotic organelles Microbial Cell 6 : 134–141.
DOI: 10.15698/mic2019.02.669
Oborník M. (2019) Gurmánův pokrm zapomnění Vesmír 98 : 112–114.
Oborník M. (2019) Endosymbiotic Evolution of Algae, Secondary Heterotrophy and Parasitism Biomolecules 9 : 266.
DOI: 10.3390/biom9070266
Oborník M. (2019) Evoluce parazitických výtrusovců z fotosyntetického předka Živa 6 : 287–289.
Sharaf A., De Michele R., Sharma A., Fakhari S., Oborník M. (2019) Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in Chromera velia Biomolecules 9 : 647.
DOI: 10.3390/biom9110647
Sharaf A., Füssy Z., Tomčala A., Richtová J., Oborník M. (2019) Isolation of plastids and mitochondria from Chromera velia Planta 250 : 1731–1741.
DOI: 10.1007/s00425-019-03259-3
Sharaf A., Gruber A., Jiroutová K., Oborník M. (2019) Characterization of Aminoacyl-tRNA Synthetases in Chromerids Genes 10 : 582.
DOI: 10.3390/genes10080582
Smith S.R., Dupont C.L., McCarthy J.K., Boddrick J.T., Oborník M., Horák A., Füssy Z., Cihlář J., Kleessen S., Zheng H., McCrow J.P., Hixson K.K., Araújo W.L., Nunes-Nesi A., Fernie A., Nikoloski Z., Palsson B.O., Allen A.E. (2019) Evolution and regulation of nitrogen flux through compartmentalized metabolic networks in a marine diatom Nature Communications 10 : 4552.
DOI: 10.1038/s41467-019-12407-y
Brunson J.K., McKinnie S.M.K., Chekan J.R., McCrow J.P., Miles Z.D., Bertrand E.M., Bielinski V.A., Luhavaya H., Oborník M., Smith G.J., Hutchins D.A., Allen A.E., Moore B.S. (2018) Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom. Science 361 : 1356–1358.
DOI: 10.1126/science.aau0382

Documents to download:
Brunson_et al._2018_Science (pdf)
Füssy Z., Oborník M. (2018) Complex Endosymbioses I: From Primary to Complex Plastids, Multiple Independent Events. In: E. Maréchal (Ed.), Methods in Molecular Biology. Humana Press, New York 1829 : 17–35.
DOI: 10.1007/978-1-4939-8654-5_2

Documents to download:
Fussy and Obornik_2018_Chapter (pdf)
McQuaid J.B., Kustka A.B., Oborník M., Horák A., Mccrow J., Karas B.J., Zheng H., Kindeberg T., Andersson A.J., Barbeau K.A., Allen A. (2018) Carbonate-sensitive phytotransferrin controls high-affinity iron uptake in diatoms Nature 555 : 534-537.
DOI: 10.1038/nature25982
Oborník M. (2018) The Birth of Red Complex Plastids: One, Three, or Four Times? Trends in Parasitology 34 : 11.
DOI: 10.1016/
Sharaf A., Oborník M., Hammad A., El-Afifi S., Marei E. (2018) Characterization and comparative genomic analysis of virulent and temperate Bacillus megaterium bacteriophages PeerJ 6 : e5687.
DOI: 10.7717/peerj.5687

More publications


Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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