RNDr. Petr Kopáček, CSc.

Job Position: Head of Laboratory - Laboratory of Vector Immunology

Contact details

Phone: +420 387772207, +420 387775476
E-mail: kopajz@paru.cas.cz
Room: 336

Curriculum vitae

Born
14th April, 1958, České Budějovice, Czechoslovakia

ORCID Id: 0000-0003-2412-5724

Education
1982
: RNDr. (equivalent to M.Sc.) - graduated in physical chemistry at Faculty of Sciences, Charles University, Prague
1988: Postgradual course of analytical chemistry at Faculty of Sciences, Komenius University, Bratislava, Slovakia
1993: CSc. (equivalent to PhD.) in Biochemistry at Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic

Former and current positions
1982–1983
: Institute of Parasitology, Czechoslovak Academy of Sciences, České Budějovice; assistentship on human protozoan isoenzymes
1983–1985: Institute of Entomology, Czech. Acad. Sci., České Budějovice, research assistant on insect olfaction physiology
1985–1993: Laboratory of Analytical Chemistry, Biol. Center Czech. Acad. Sci., České Budějovice, research assistant on protein analysis, electrophoresis and related techniques
1993–1995: Laboratory of Analytical Chemistry; Institute of Entomology, Czech Acad. Sci., České Budějovice, research worker on proteins involved in immune system of insects
1995–1996: Galena (IVAX), Research Unit, České Budějovice, research specialist on drug binding proteins
1996: Institute of Parasitology, Biology Centre, Acad. Sci. Czech Rep. and Faculty of Sciences, University of South Bohemia, České Budějovice, senior scientist on immune proteins in ticks

Scientific stays, fellowships and courses
October–November 1989
: Stay at Technical University Munich, Germany by Prof. B. Radola, study of isoelectric focusing and two-dimensional electrophoresis
October 1991–March 1992: Stay at University of Uppsala, Sweden, Institute of Physiological Botany by Prof. K. Söderhäll; study on crayfish plasma proteins
May 1994–June 1995: Fellowship from Alexander von Humboldt Foundation, Institute of Zoologie, Free University Berlin by Prof. P. Götz and Prof. M. Schweiger, study on insect prophenoloxidase systém
April 1997–June 1997: Stay at University of Arizona, Tucson, Center of Insect Science by Prof. John H. Law, within the BRAVO! program, work on tick ferritins
June 2000 – Course - Biology of Disease Vectors, Colorado State University, Fort Collins, Co
July 2000 – August 2000: Summer laboratory at Woods Hole, Ma. Co-operation with Prof. Peter B. Armstrong (University of California), on alpha-2-macroglobulin receptor from Limulus
September 2002: HHMI/NRC sponsored course: "Genome-wide Approaches to Understanding Bacterial Pathogenesis”, Institute of Microbiology, Prague, Czech Rep.
October–November 2003: EMBO practical course: "Integrated Molecular Diagnostics for Tick-borne Pathogens Using RLB Hybridization and Microarray Based Biochips", University of Pretoria, South Africa
November 2006: Scientific visit at Departamento de Parasitologia, Universidade de Sao Paulo, Instituto de Ciencias Biomedicas (Prof. Dr. Sirlei Daffre) and Departmento de Biochuimica Medica, ICB, Universidade Federal do Rio de Janeiro (Prof. Dr. Pedro L. Oliveira)

Projects solved
a) principal investigator
1997–1999 – Immune system of a tick: Identification and characterization of proteins involved in clotting and melanization of tick hemolymph (A6022701, Grant Agency, Academy Sciences CR)
2000–2002 – Humoral factors and cellular events involved in pathogen transmission by ticks (206/00/266 Grant Agency CR)
2003–2005 – Immune reactions within the gut of the soft tick Ornithodoros moubata (A6022307 Grant Agency, Academy Sciences CR)
2005–2008 – The role of α2-macroglobulin in pathogen transmission by the tick Ixodes ricinus (IAA600220603, Grant Agency, Academy Sciences CR).
2005–2008 – Peptidases in the gut of the hard tick Ixodes ricinus: characterization and potential for vector-control (206/060/865, Grant Agency CR)
2009–2012 – The digestive system of ticks – the target for rational development of vaccine against tick and tick-borne pathogens (IAA600960910 Grant Agency, Academy Sciences CR)
2010–2012 – Functional genomics of the complement-like molecules in the tick Ixodes ricinus (P506/10/2136 Grant Agency CR)
2013–2017 – The role of hemoglobin in tick metabolism and pathogen transmission (13-11043S, Grant Agency CR) (GACR award 2018 – see below)
2018–2020 Nutritional factors essential for tick development and reproduction (18-01832S, Grant Agency CR)
2020–2022 – The role of Toll pathway in the regulation of tick immune response against tick-transmitted pathogens (20-05736S, Grant Agency CR)

b) co-principal investigator
2010–2013 – Structural proteomics of tick proteolytic systems (P207/10/2183 Grant Agency CR)
2011–2014 – Development of a vaccine protecting against transmission of tick-borne diseases based on ferritin 2 for veterinary and human application (FR-TI/3-156, Ministry of Industry and Trade CR)
2019–2022 – Midgut microbial ecology, epithelial immunity, and vector competence of Ixodes ricinus and Amblyomma americanum (19-04301S, Grant Agency CR)

Publishing activity (till November  2020)
Papers published in peer-reviewed journals – 75
Chapters in edited book – 4
Patents – 3
Number of citation (without self-citation) ~ 2350
Hirsch index – 33

Teaching activities
Supervisor of 6 and co-supervisor of 2 defended Ph.D. theses and ~ 25 undergraduate diploma works (Bc., MSc.).
September 2019 – a tutor in the 2 weeks’ course “Escola de Altos Estudos” (High Studies School Program granted by Brazilian Federal Agency for Post-graduate Educatio, CAPES) – Univ. Fed. Rio de Janeiro, and Univ Fed. Rio Grande do Sul, Porto Alegre, Brasil.

Other activities
Since 2012
– a member of the Editorial Advisory Board of Developmental & Comparative Immunology (Elsevier)
2017–2021  – member of the GACR panel 502

Awards:
2018
– Award from the President of the Czech Science Foundation (GAČR) in Biological & Agricultural Science for the excellent results achieved in the project  "The role of hemoglobin in tick metabolism and transmission of tick-borne pathogens".

Publications
Total found: 73 records
Garcia Guizzo M., Neupane S., Kučera M., Perner J., Frantová H., da Silva V.T., de Oliveira P.L., Kopáček P., Zurek L. (2020) Poor unstable midgut microbiome of hard ticks contrasts with abundant and stable monospecific microbiome in ovaries Frontiers in Cellular and Infection Microbiology 10 : 211.
DOI: 10.3389/fcimb.2020.00211
Gobl J., Sinha D., Šíma R., Perner J., Kopáček P., Valdés J. J., Rego R. O. M., Cabezas-Cruz A. (2020) Histone Methyltransferase DOT1L Is Involved in Larval Molting and Second Stage Nymphal Feeding in Ornithodoros moubata Vaccines 8 : 157.
DOI: 10.3390/vaccines8020157
Perner J., Helm D., Haberkant P., Hatalová T., Kropáčková S., Ribeiro J., Kopáček P. (2020) The central role of salivary metalloproteases in host acquired resistance to tick feeding Frontiers in Cellular and Infection Microbiology 10 : 563349.
DOI: 10.3389/fcimb.2020.563349
Trentelman J.J., Šíma R., Krezdorn N., Tomás-Cortázar J., Barriales D., Takumi K., Butler J.M., Sprong H., Klouwens M.J., Urbanová V., Mahmood S., Winter P., Kopáček P., Anguita J., Hajdušek O., Hovius J. (2020) A combined transcriptomic approach to identify candidates for an anti-tick vaccine blocking B. afzelii transmission Scientific Reports 10 : 20061.
DOI: 10.1038/s41598-020-76268-y
Perner J., Gasser R.B., Oliveira P.L., Kopáček P. (2019) Haem Biology in Metazoan Parasites – ‘The Bright Side of Haem’ Trends in Parasitology 35 : 213–225.
DOI: 10.1016/j.pt.2019.01.001
Pospíšilová Pospíšilová, Urbanová V., Hes O., Kopáček P., Hajdušek O., Šíma R. (2019) Tracking of Borrelia afzelii transmission from infected Ixodes ricinus nymphs to mice Infection and Immunity 87 : e00896-18.
DOI: 10.1128/IAI.00896-18
Rego R. O. M., Trentelman J.J., Anguita J., Nijhof A., Sprong H., Klempa B., Hajdušek O., Tomás-Corázar J., Azagi T., Strnad M., Knorr S. , Šíma R., Jalovecká M., Havlíková S., Ličková M., Sláviková M., Kopáček P., Grubhoffer L., Hovius J. (2019) Counterattacking the tick bite: towards a rational design of anti-tick vaccines targeting pathogen transmission Parasites & Vectors 12 : 229.
DOI: 10.1186/s13071-019-3468-x
Hánová I., Brynda J., Houštecká R., Alam N., Sojka D., Kopáček P., Marešová L., Vondrášek J., Horn M., Schueler-Furman O., Mareš M. (2018) Novel structural mechanism of allosteric regulation of aspartic peptidases via an evolutionarily conserved exosite Cell Chemical Biology 25 : 1-12.
DOI: 10.1016/j.chembiol.2018.01.001
Hartmann D., Šíma R., Konvičková J., Perner J., Kopáček P., Sojka D. (2018) Multiple legumain isoenzymes in ticks International Journal for Parasitology 48 : 167-178.
DOI: 10.1016/j.ijpara.2017.08.011
Jalovecká M., Hajdušek O., Sojka D., Kopáček P., Malandrin L. (2018) The Complexity of Piroplasms Life Cycles Frontiers in Cellular and Infection Microbiology 8 : 248.
DOI: 10.3389/fcimb.2018.00248
Knorr S. , Anguita J., Cortazar J.T., Hajdušek O., Kopáček P., Trentelman J.J., Kershaw O., Hovius J., Nijhof A. (2018) Preliminary Evaluation of Tick Protein Extracts and Recombinant Ferritin 2 as Anti-tick Vaccines Targeting Ixodes ricinus in Cattle. Frontiers in Physiology 9 : 1969.
DOI: 10.3389/fphys.2018.01696
Kopáček P., Perner J., Sojka D., Šíma R., Hajdušek O. (2018) Molecular Targets to Impair Blood Meal Processing in Ticks. In: Ch. Q. Meng, A. E. Sluder and P.M Selzer (Eds.), Ectoparasites: Drug Discovery Against Moving Targets Wiley 139–165.
DOI: 10.1002/9783527802883
Perner J., Kotál J., Hatalová T., Urbanová V., Bartošová-Sojková P., Brophy P.M., Kopáček P. (2018) Inducible glutathione S-transferase (IrGST1) from the tick Ixodes ricinus is a haem-binding protein Insect Biochemistry and Molecular Biology DOI: 10.1016/j.ibmb.2018.02.002
Perner J., Kropáčková S., Kopáček P., Ribeiro J. (2018) Sialome diversity of ticks revealed by RNAseq single tick salivary glands PLOS Neglected Tropical Diseases 12 : e0006410.
DOI: 10.1371/journal.pntd.0006410
Urbanová V., Hajdušek O., Šíma R., Franta Z., Grunclová L., Hönig Mondeková H., Bartošová-Sojková P., Jalovecká M., Kopáček P. (2018) IrC2/Bf e A yeast and Borrelia responsive component of the complement system from the hard tick Ixodes ricinus Developmental and Comparative Immunology 79 : 86-94.
DOI: 10.1016/j.dci.2017.10.012
Hönig Mondeková H., Šíma R., Urbanová V., Kovář V., Rego R. O. M., Grubhoffer L., Kopáček P., Hajdušek O. (2017) Characterization of Ixodes ricinus Fibrinogen-Related Proteins (Ixoderins) Discloses Their Function in the Tick Innate Immunity Frontiers in Cellular and Infection Microbiology 7 : 509.
DOI: 10.3389/fcimb.2017.00509
Urbanová V., Hajdušek O., Hönig Mondeková H., Šíma R., Kopáček P. (2017) Tick Thioester-Containing Proteins and Phagocytosis Do Not Affect Transmission of Borrelia afzelii from the Competent Vector Ixodes ricinus Frontiers in Cellular and Infection Microbiology 7 : 73.
DOI: 10.3389/fcimb.2017.00073
Zavašnik-Bergant T., Vidmar R., Sekirnik A., Fonović M., Salát J., Grunclová L., Kopáček P., Turk B. (2017) Salivary tick cystatin OmC2 targets lysosomal cathepsins S and C in human dendritic cells Frontiers in Cellular and Infection Microbiology 7 : 288.
DOI: 10.3389/fcimb.2017.00288
Chmelař J., Kotál J., Karim S., Kopáček P., Francischetti I., Pedra J., Kotsyfakis M. (2016) Sialomes and Mialomes: A Systems-Biology View of Tick Tissues and Tick-Host Interactions Trends in Parasitology 32 : 242-254.
DOI: 10.1016/j.pt.2015.10.002
Hajdušek O., Šíma R., Perner J., Loosová G., Harcubová A., Kopáček P. (2016) Tick iron and heme metabolism - New target for an anti-tick intervention Ticks and tick-borne diseases 7 : 565-572.
DOI: 10.1016/j.ttbdis.2016.01.006

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CONTACT

Biology Centre CAS
Institute of Parasitology
Branišovská 1160/31
370 05 České Budějovice

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